Standard name
Human Ortholog
Description Polyphosphate phosphatase; hydrolyzes diphosphorylated inositol polyphosphates and diadenosine polyphosphates; high specificity for diadenosine hexa- and pentaphosphates; contains endopolyphosphatase activity with a high affinity for polyphosphates, an activity also observed for its human DIPP homologs; possesses mRNA decapping activity; nudix hydrolase family member; protein abundance increases in response to DNA replication stress
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.98 0.97 0.99 0.98 0.9 0.9 0.69 – 0.72 0.68 0.7 0.66 0.99 0.98 0.99 0.93 0.97 0.95 0.94 0.95 0.93 0.96 0.97 0.95
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.13 0 – 0.15 0.08 0.13 0.16 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 – 0.05 0.06 0.05 0.09 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0.13 0.06 0.3 – 0.15 0.24 0.17 0.12 0 0 0 0.11 0 0.07 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 2 0 0 0 0 0 0 0 0 2 1 0 0 1 0
Bud 0 1 0 0 0 2 0 – 2 1 1 0 0 0 0 0 0 0 1 1 0 1 3 4
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 1 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 1 0 0 2 2 4 – 3 11 4 2 2 4 4 7 4 2 0 0 0 0 1 0
Cytoplasm 492 532 134 102 171 178 168 – 191 200 153 131 631 1127 1069 185 101 57 470 523 132 445 827 825
Endoplasmic Reticulum 3 8 1 0 1 0 0 – 5 3 1 8 2 5 5 3 1 2 1 1 0 2 4 8
Endosome 0 0 0 0 0 0 0 – 0 1 1 0 0 1 0 0 0 0 3 2 0 1 1 5
Golgi 4 2 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 7 2 0 5 1 1
Mitochondria 4 2 0 0 1 25 6 – 39 23 28 31 0 0 1 3 1 0 1 3 3 1 2 2
Nucleus 1 7 0 1 2 2 6 – 12 18 11 17 4 3 1 6 3 0 1 3 1 0 2 2
Nuclear Periphery 0 0 0 0 0 0 0 – 0 1 1 0 0 0 0 0 0 0 0 1 0 0 0 1
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 2
Vac/Vac Membrane 9 5 1 2 24 11 73 – 40 71 36 23 3 18 31 21 3 4 1 5 0 3 2 4
Unique Cell Count 503 549 136 104 191 198 243 266 296 218 197 635 1148 1085 200 104 60 498 551 142 466 851 865
Labelled Cell Count 513 558 136 105 201 220 257 292 331 236 213 642 1159 1111 225 113 65 498 551 142 466 851 865


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 10.9 9.9 10.2 9.3 10.7 7.1 9.4 – 8.2 8.7 8.1 8.3 12.7 13.5 13.6 10.7 11.9 11.7 10.5 11.3 12.1
Std Deviation (1e-4) 2.0 1.8 1.3 1.8 1.5 1.8 1.6 – 1.7 1.6 1.6 1.5 2.1 2.3 2.2 2.2 1.9 2.5 2.1 2.0 2.2
Intensity Change (Log2) – – – -0.14 0.07 -0.53 -0.12 – -0.31 -0.24 -0.34 -0.31 0.31 0.39 0.41 0.07 0.22 0.19 0.04 0.14 0.24


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cytoplasm -0.3 -3.2 -3.1 -6.8 – -6.4 -7.1 -6.6 -7.1 1.0 -0.3 0 -2.5 -0.8 -1.4
Endoplasmic Reticulum 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 4.3 0 – 4.7 3.3 4.4 4.9 0 0 0 0 0 0
Nucleus 0 0 0 0 – 0 2.9 0 3.5 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Vacuole 0 4.0 0 6.9 – 4.5 6.0 4.7 3.8 0 0 0 3.6 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 3.241 4.1792 3.6914 2.9272 3.5959 3.6332 6.4654 7.3792 7.2154 6.3263 6.4989 6.9261 7.4928 8.8583 8.4029 7.9062 8.223 8.324
Actin 0.0138 0.0004 0.0068 0.0047 0.01 0.0075 0.0004 0.0004 0.0003 0.0013 0.0004 0.0003 0.0204 0.0017 0.0017 0.0172 0.0076 0.0022
Bud 0.0007 0.0002 0.0004 0.001 0.0018 0.0001 0.0009 0.0003 0.0025 0.0002 0.0002 0.0009 0.0004 0.0002 0.0002 0.0002 0.0002 0.0001
Bud Neck 0.0037 0.0003 0.0008 0.0009 0.0044 0.0014 0.0005 0.0004 0.0004 0.0011 0.0037 0.0016 0.0024 0.0003 0.0005 0.0005 0.0006 0.001
Bud Periphery 0.0009 0 0.0004 0.0007 0.0025 0.0001 0.0002 0.0001 0.0004 0.0001 0.0001 0.0002 0.0003 0.0001 0.0001 0.0001 0.0002 0
Bud Site 0.0039 0.0025 0.0006 0.0006 0.0067 0.0002 0.0017 0.0014 0.0005 0.0002 0.0002 0.0001 0.0006 0.0013 0.001 0.0003 0.001 0.0001
Cell Periphery 0.0002 0 0.0001 0.0001 0.0003 0 0.0001 0 0.0001 0 0 0 0.0001 0.0001 0 0.0001 0 0
Cytoplasm 0.8145 0.9434 0.9061 0.8874 0.6556 0.9181 0.9168 0.9388 0.9386 0.9009 0.9299 0.9431 0.8647 0.9369 0.9398 0.9282 0.9369 0.9659
Cytoplasmic Foci 0.0248 0.0068 0.0095 0.0121 0.0128 0.0091 0.0047 0.0055 0.0032 0.0066 0.0131 0.0049 0.0237 0.0067 0.0042 0.0113 0.0075 0.0047
Eisosomes 0.0001 0 0.0001 0 0.0002 0 0 0 0 0 0 0 0.0003 0 0 0.0001 0 0
Endoplasmic Reticulum 0.0073 0.0029 0.0054 0.002 0.0166 0.0031 0.0024 0.0017 0.0019 0.0017 0.002 0.0021 0.0057 0.0016 0.002 0.0051 0.0015 0.0019
Endosome 0.0394 0.0024 0.0146 0.0025 0.0507 0.0061 0.0032 0.0025 0.0017 0.0025 0.0076 0.0021 0.0156 0.0012 0.002 0.0065 0.0081 0.0016
Golgi 0.0064 0.0002 0.0019 0.0007 0.0035 0.0013 0.0001 0.0001 0.0001 0.0001 0.0002 0.0001 0.0024 0.0003 0.0003 0.0022 0.0032 0.0001
Lipid Particles 0.0037 0 0.0039 0.0033 0.005 0.0016 0 0 0 0 0.0001 0 0.0011 0.0002 0.0008 0.0033 0.0037 0
Mitochondria 0.0037 0.0001 0.0087 0.0006 0.0029 0.0004 0.0003 0.0008 0.0002 0.0003 0.0003 0.0002 0.0013 0.0003 0.0002 0.0002 0.0007 0.0001
None 0.0285 0.0244 0.0158 0.0117 0.0561 0.0139 0.0329 0.016 0.0232 0.0224 0.0161 0.0112 0.0348 0.0361 0.0294 0.0128 0.0047 0.0071
Nuclear Periphery 0.0168 0.0016 0.0045 0.0019 0.0519 0.0031 0.0025 0.0022 0.0016 0.0024 0.0017 0.0021 0.0035 0.001 0.0017 0.0019 0.0019 0.0013
Nucleolus 0.0015 0.0001 0.0003 0.0001 0.0092 0.0001 0.0001 0.0002 0.0001 0.0001 0.0001 0.0001 0.0005 0.0001 0.0001 0 0.0002 0
Nucleus 0.012 0.0111 0.0141 0.0173 0.0577 0.0221 0.0297 0.0244 0.0225 0.0551 0.0203 0.0279 0.0089 0.0042 0.0067 0.0078 0.0061 0.0123
Peroxisomes 0.0027 0.0002 0.0009 0.0485 0.0016 0.0043 0.0002 0.0002 0.0001 0.0002 0.0002 0.0001 0.0046 0.0005 0.0009 0.0006 0.009 0.0001
Punctate Nuclear 0.0098 0.0021 0.0022 0.0021 0.0381 0.0059 0.0023 0.0036 0.0022 0.0036 0.0021 0.0023 0.0068 0.0069 0.0079 0.001 0.0064 0.001
Vacuole 0.0044 0.0009 0.0022 0.0017 0.0092 0.0011 0.0008 0.0009 0.0006 0.001 0.0015 0.0006 0.0015 0.0003 0.0004 0.0006 0.0003 0.0003
Vacuole Periphery 0.0014 0.0001 0.0008 0.0002 0.003 0.0002 0.0001 0.0001 0.0001 0.0001 0.0003 0.0001 0.0006 0.0001 0.0001 0.0001 0.0001 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 31.1366 29.5739 28.3995 46.0689 49.2009 40.8442 40.8956 46.8933 62.2793 52.041
Translational Efficiency 3.0168 2.5004 2.9035 2.4839 1.7938 2.5858 1.8682 2.296 1.647 1.7539

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘
Standard name
Human Ortholog
Description Polyphosphate phosphatase; hydrolyzes diphosphorylated inositol polyphosphates and diadenosine polyphosphates; high specificity for diadenosine hexa- and pentaphosphates; contains endopolyphosphatase activity with a high affinity for polyphosphates, an activity also observed for its human DIPP homologs; possesses mRNA decapping activity; nudix hydrolase family member; protein abundance increases in response to DNA replication stress
Localization
Cell Percentages cytoplasm (100%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Ddp1

Ddp1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ddp1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available