Standard name
Human Ortholog
Description Swi2/Snf2-related translocase, SUMO-Targeted Ubiquitin Ligase (STUbL); required for maintenance of NHEJ inhibition at telomeres; functions at telomeres to translocate and ubiquitinylate poly-sumoylated Rap1p for proteosomal degradation; plays role in antagonizing silencing during mating-type switching; only known STUbL with a translocase activity; contains RING finger domain; relocalizes from nucleus to cytoplasm upon DNA replication stress
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.2 0.2 0.49 0.23 0.37 0.51 0.49 0.56 0.38 – 0.34 0.3 0.39 0.49 0.6 0.55 0.51 0.54 0.07 0.1 0.16 0.12 0.19 0.2
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0.05 0 0.06 0 0 0 0 0 0
Endosome 0 0 0 0 0.1 0 0 0 0 – 0 0 0 0 0 0.14 0.15 0.16 0 0 0 0 0 0.06
Golgi 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0.3 0.23 0.6 0.34 0.54 0.54 0.51 0.69 – 0.72 0.77 0.3 0.14 0.41 0.06 0 0 0.06 0 0.11 0 0.06 0.07
Nucleus 0.51 0.3 0.22 0.27 0.14 0.1 0.08 0.06 0.09 – 0.09 0.11 0.26 0.15 0 0.1 0 0 0.47 0.46 0.25 0.45 0.21 0.1
Nuclear Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0.05 0 0 0 0 – 0 0 0 0 0 0 0 0 0.05 0 0 0 0.09 0.21
Vac/Vac Membrane 0.28 0.43 0.08 0.07 0.16 0.06 0.07 0 0.06 – 0 0 0.22 0.3 0.23 0.3 0.42 0.34 0.21 0.29 0.33 0.19 0.31 0.26
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 1 0 1 0 0 1
Bud 0 0 0 1 1 6 5 9 9 – 6 11 0 0 2 0 0 1 0 2 5 1 1 4
Bud Neck 0 0 0 0 2 0 1 3 1 – 0 1 0 0 0 0 0 1 1 1 1 1 1 0
Bud Site 0 0 1 1 0 2 3 2 4 – 6 11 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 1 1 0 1 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 20 44 58 33 72 111 147 205 97 – 104 100 29 60 70 105 99 110 11 28 30 9 31 38
Endoplasmic Reticulum 0 3 1 1 3 4 0 0 1 – 0 2 0 2 0 9 7 13 0 0 0 0 1 0
Endosome 2 3 5 1 19 0 1 7 1 – 0 2 0 0 1 26 29 32 6 2 0 2 6 11
Golgi 3 1 0 0 0 0 0 0 0 – 0 0 0 0 0 2 0 3 3 1 0 0 0 0
Mitochondria 3 67 27 88 65 117 164 188 176 – 218 253 22 17 48 12 5 8 9 11 22 2 9 12
Nucleus 50 66 26 39 28 21 25 23 22 – 26 35 19 18 1 19 5 7 82 128 47 37 34 19
Nuclear Periphery 1 0 0 0 1 0 1 1 0 – 1 1 0 0 0 1 0 0 3 0 0 0 0 0
Nucleolus 1 0 0 2 2 2 1 2 0 – 3 1 0 0 0 0 1 0 3 1 0 1 3 4
Peroxisomes 0 0 1 0 0 0 0 0 0 – 0 0 0 0 0 0 1 0 1 0 0 0 0 0
SpindlePole 1 0 3 5 10 1 4 6 4 – 3 3 0 0 0 2 2 2 8 12 6 2 14 40
Vac/Vac Membrane 27 96 9 10 31 12 22 14 16 – 10 10 16 37 27 57 83 70 35 80 63 16 49 50
Unique Cell Count 98 221 119 146 194 217 302 369 254 302 329 74 122 117 192 196 204 174 279 193 83 161 192
Labelled Cell Count 108 280 131 181 235 277 374 461 331 377 430 86 134 149 233 232 247 174 279 193 83 161 192


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.1 3.8 4.9 3.9 5.1 3.8 3.3 3.7 3.4 – 3.1 2.7 3.9 4.4 3.7 5.6 6.1 6.5 4.7 4.1 4.9
Std Deviation (1e-4) 1.0 0.8 2.5 2.0 3.6 2.1 1.7 2.3 2.3 – 1.8 1.4 0.8 1.2 1.1 1.7 2.1 2.5 2.0 1.5 1.6
Intensity Change (Log2) – – – -0.33 0.07 -0.36 -0.55 -0.4 -0.54 – -0.64 -0.85 -0.31 -0.16 -0.42 0.21 0.32 0.42 -0.05 -0.24 0.02


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – 0 0 0
Bud Neck – – – – – – – – – – – – 0 0 0
Bud Site – – – – – – – – – – – – 0 0 0
Cell Periphery – – – – – – – – – – – – 0 0 0
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – 0 0 0
Endosome – – – – – – – – – – – – 0 0 0
Golgi – – – – – – – – – – – – 0 0 0
Mitochondria – – – – – – – – – – – – 0 0 0
Nuclear Periphery – – – – – – – – – – – – 0 0 0
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – 0 0 0
Peroxisomes – – – – – – – – – – – – 0 0 0
SpindlePole – – – – – – – – – – – – 0 0 0
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – 0 0 0
Cytoplasm – – – – – – – – – – – – 0 0 0
Nucleus – – – – – – – – – – – – 0 0 0
Vacuole – – – – – – – – – – – – 4.6 6.6 5.4

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration – – – – – – – – – – – – – – – – – –
Actin 0.0276 0.0007 0.0198 0.0018 0.0647 0.0036 0.0473 0.0006 0.0055 0.0014 0.007 0.0023 0.0001 0.0097 0.0022 0.0005 0.0006 0.0008
Bud 0.0002 0.0002 0.0003 0.0003 0.0006 0.0002 0.0007 0.0003 0.0004 0.0003 0.0004 0.0043 0 0.0001 0.0003 0.0008 0.0001 0.0002
Bud Neck 0.0106 0.0019 0.0014 0.0012 0.0025 0.0167 0.0032 0.0005 0.0011 0.0023 0.0008 0.0098 0.0001 0.0001 0.0005 0.0057 0.0016 0.0035
Bud Periphery 0.0003 0.0002 0.0004 0.001 0.0012 0.0003 0.0013 0.0002 0.0009 0.0006 0.0007 0.0013 0 0.0002 0.0006 0.0027 0.0001 0.0001
Bud Site 0.0013 0.0069 0.0018 0.0012 0.0023 0.0025 0.0055 0.0014 0.0018 0.0015 0.0007 0.0007 0.0003 0.0002 0.0006 0.0008 0.0003 0.0003
Cell Periphery 0.0002 0.0003 0.0002 0.0003 0.0002 0.0001 0.0002 0 0.0001 0.0001 0.0001 0.0001 0 0 0.0001 0.0003 0 0.0002
Cytoplasm 0.0352 0.0783 0.0378 0.0651 0.0389 0.0562 0.0337 0.0637 0.0206 0.072 0.0412 0.0368 0.0218 0.0443 0.0877 0.0413 0.0484 0.0934
Cytoplasmic Foci 0.0114 0.0036 0.0092 0.0022 0.0211 0.0045 0.0254 0.0014 0.0053 0.024 0.0055 0.0066 0.0001 0.0033 0.0014 0.0008 0.0022 0.0148
Eisosomes 0.0004 0.0001 0.0004 0.0001 0.0006 0 0.0004 0 0.0001 0.0001 0.0002 0 0 0.001 0.0001 0 0 0.0002
Endoplasmic Reticulum 0.0046 0.007 0.0047 0.0102 0.0038 0.0038 0.0066 0.0028 0.0018 0.0032 0.0026 0.0018 0.0008 0.0006 0.0025 0.0039 0.0022 0.0021
Endosome 0.013 0.0078 0.012 0.0098 0.0241 0.008 0.0407 0.0023 0.0077 0.0063 0.032 0.004 0.0006 0.0005 0.0039 0.0063 0.0057 0.0024
Golgi 0.0041 0.0002 0.0038 0.0004 0.0079 0.0021 0.0124 0.0001 0.0041 0.0007 0.0322 0.0023 0 0.0005 0.0006 0.0001 0.0001 0.0001
Lipid Particles 0.0076 0.0017 0.008 0.0015 0.0542 0.0062 0.0204 0.0002 0.0047 0.0018 0.0078 0.0053 0 0.0009 0.001 0.0003 0.0003 0.0008
Mitochondria 0.0144 0.0009 0.0045 0.0099 0.007 0.0017 0.0152 0.0011 0.0039 0.0039 0.0713 0.008 0.0005 0.0014 0.0021 0.0022 0.001 0.0012
None 0.0433 0.116 0.0615 0.1026 0.0532 0.0379 0.0904 0.1038 0.0823 0.1355 0.1301 0.0626 0.0028 0.4236 0.2974 0.3395 0.0313 0.2548
Nuclear Periphery 0.0303 0.0437 0.0297 0.0218 0.0312 0.022 0.0477 0.0429 0.0406 0.085 0.0219 0.0119 0.0294 0.0054 0.0466 0.1012 0.0381 0.0101
Nucleolus 0.0096 0.0114 0.0094 0.0132 0.0084 0.0196 0.0067 0.0075 0.0116 0.0242 0.0051 0.0143 0.0024 0.0011 0.0077 0.0062 0.0138 0.0122
Nucleus 0.7283 0.6859 0.7544 0.736 0.6079 0.7842 0.5842 0.7295 0.75 0.5349 0.6228 0.7721 0.9331 0.5003 0.5223 0.4556 0.7799 0.5762
Peroxisomes 0.0061 0.0001 0.0081 0.0004 0.0335 0.0044 0.0183 0.0002 0.0046 0.0146 0.0047 0.0009 0 0.0037 0.001 0.0001 0.0002 0.0002
Punctate Nuclear 0.0479 0.0244 0.0302 0.0147 0.0295 0.022 0.0301 0.0396 0.0505 0.0752 0.0084 0.0533 0.0071 0.0028 0.0189 0.0096 0.0725 0.0245
Vacuole 0.0027 0.0074 0.0019 0.005 0.006 0.003 0.0068 0.0011 0.0015 0.0076 0.0022 0.0013 0.0006 0.0003 0.0012 0.0179 0.0012 0.0018
Vacuole Periphery 0.0008 0.0011 0.0008 0.0014 0.0014 0.0008 0.0029 0.0005 0.0009 0.0046 0.0023 0.0003 0.0003 0.0001 0.001 0.0042 0.0005 0.0003

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 15.8927 15.4248 12.3495 17.6947 18.9865 19.0606 19.8541 20.2907 17.8937 22.5356
Translational Efficiency 0.6747 0.5712 0.6031 0.452 0.4581 0.4914 0.4184 0.4164 0.461 0.4566

Hit Data

Dataset Hit
Protein Concentration –
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Swi2/Snf2-related translocase, SUMO-Targeted Ubiquitin Ligase (STUbL); required for maintenance of NHEJ inhibition at telomeres; functions at telomeres to translocate and ubiquitinylate poly-sumoylated Rap1p for proteosomal degradation; plays role in antagonizing silencing during mating-type switching; only known STUbL with a translocase activity; contains RING finger domain; relocalizes from nucleus to cytoplasm upon DNA replication stress
Localization
Cell Percentages nucleus (63%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-3

Uls1

Uls1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Uls1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available