Standard name | |
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Human Ortholog | |
Description | Protein involved in biosynthesis of the coenzyme lipoic acid; has similarity to E. coli lipoic acid synthase |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 1 | 5 | 3 | 1 | 1 | 0 | 0 | 0 | 2 | 2 | 0 | 0 | 3 | 2 | 9 | 6 | 12 | 7 | 3 | 4 | 3 | 6 | 10 | 11 |
Bud | 0 | 1 | 6 | 6 | 6 | 6 | 7 | 6 | 8 | 10 | 7 | 7 | 1 | 0 | 3 | 9 | 8 | 4 | 1 | 0 | 0 | 2 | 4 | 2 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 |
Bud Site | 1 | 1 | 0 | 0 | 2 | 2 | 6 | 3 | 3 | 3 | 1 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 1 | 8 | 5 | 4 | 3 | 3 | 6 | 4 | 3 | 3 | 1 | 1 | 13 | 16 | 23 | 9 | 13 | 7 | 0 | 0 | 1 | 0 | 1 | 1 |
Cytoplasm | 4 | 1 | 3 | 6 | 4 | 1 | 0 | 6 | 4 | 2 | 4 | 1 | 10 | 10 | 6 | 5 | 10 | 7 | 0 | 0 | 1 | 0 | 0 | 0 |
Endoplasmic Reticulum | 2 | 3 | 2 | 0 | 0 | 0 | 2 | 1 | 3 | 0 | 2 | 0 | 0 | 5 | 4 | 2 | 6 | 1 | 0 | 0 | 1 | 0 | 1 | 1 |
Endosome | 1 | 1 | 2 | 0 | 4 | 0 | 1 | 1 | 2 | 0 | 0 | 1 | 11 | 17 | 13 | 9 | 6 | 6 | 4 | 7 | 3 | 9 | 13 | 12 |
Golgi | 1 | 5 | 7 | 2 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 135 | 252 | 228 | 22 | 27 | 9 | 6 | 22 | 7 | 32 | 45 | 37 |
Mitochondria | 233 | 231 | 221 | 132 | 169 | 249 | 281 | 257 | 320 | 305 | 271 | 285 | 398 | 353 | 388 | 7 | 9 | 8 | 204 | 205 | 192 | 240 | 294 | 300 |
Nucleus | 0 | 0 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 2 | 2 | 1 | 3 | 0 | 4 | 2 | 0 | 4 | 0 | 0 | 0 | 0 | 4 | 0 |
Nuclear Periphery | 16 | 14 | 8 | 6 | 3 | 30 | 29 | 34 | 43 | 28 | 28 | 32 | 22 | 20 | 23 | 6 | 14 | 14 | 0 | 1 | 0 | 1 | 4 | 11 |
Nucleolus | 16 | 7 | 10 | 2 | 13 | 32 | 26 | 29 | 36 | 28 | 19 | 36 | 8 | 8 | 7 | 9 | 10 | 10 | 2 | 1 | 0 | 2 | 3 | 2 |
Peroxisomes | 4 | 3 | 21 | 3 | 12 | 6 | 7 | 6 | 0 | 10 | 1 | 1 | 8 | 11 | 16 | 12 | 18 | 17 | 11 | 11 | 17 | 14 | 10 | 26 |
SpindlePole | 1 | 0 | 1 | 0 | 1 | 1 | 4 | 3 | 6 | 4 | 1 | 1 | 3 | 5 | 8 | 3 | 3 | 1 | 0 | 0 | 0 | 2 | 2 | 2 |
Vac/Vac Membrane | 8 | 16 | 4 | 3 | 9 | 12 | 7 | 6 | 4 | 9 | 9 | 16 | 50 | 99 | 60 | 18 | 23 | 15 | 2 | 3 | 1 | 9 | 20 | 10 |
Unique Cell Count | 242 | 245 | 234 | 138 | 188 | 266 | 302 | 285 | 343 | 332 | 289 | 304 | 467 | 544 | 543 | 101 | 132 | 93 | 237 | 261 | 232 | 327 | 421 | 424 |
Labelled Cell Count | 289 | 296 | 293 | 165 | 229 | 343 | 377 | 358 | 435 | 406 | 346 | 384 | 665 | 798 | 792 | 119 | 159 | 110 | 237 | 261 | 232 | 327 | 421 | 424 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 10.1 | 11.2 | 9.9 | 9.3 | 9.2 | 8.0 | 8.1 | 8.2 | 7.5 | 7.9 | 7.4 | 7.5 | 12.0 | 11.5 | 10.7 | 12.7 | 12.3 | 12.6 | 12.2 | 12.7 | 13.6 |
Std Deviation (1e-4) | 2.3 | 2.8 | 1.8 | 1.7 | 2.0 | 2.0 | 2.1 | 2.3 | 2.1 | 1.9 | 2.1 | 1.9 | 2.0 | 1.9 | 1.7 | 3.4 | 2.9 | 3.5 | 2.9 | 2.7 | 2.7 |
Intensity Change (Log2) | – | – | – | -0.1 | -0.11 | -0.31 | -0.3 | -0.28 | -0.4 | -0.34 | -0.42 | -0.41 | 0.27 | 0.22 | 0.1 | 0.35 | 0.31 | 0.35 | 0.3 | 0.36 | 0.46 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3.6 | 0 |
Bud | 0 | 0.4 | -0.2 | -0.2 | -0.3 | -0.2 | 0.3 | -0.1 | -0.2 | 0 | 0 | 0 | 0 | 1.7 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0.5 | 0.6 | 1.4 | 0 | 3.3 | 0 |
Cytoplasm | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1.9 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 8.1 | 11.8 | 10.9 | 5.6 | 5.5 | 0 |
Mitochondria | 0.5 | -1.8 | -0.4 | -0.7 | -1.8 | -0.6 | -1.2 | -0.3 | -0.3 | -3.6 | -8.6 | -7.1 | -15.8 | -16.7 | -15.3 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0.5 | 0 | 3.3 | 2.8 | 3.5 | 3.8 | 2.4 | 2.8 | 3.1 | 0.8 | 0.2 | 0.5 | 0 | 2.8 | 3.8 |
Nucleolus | 0 | 1.2 | 3.1 | 2.0 | 2.5 | 2.7 | 1.9 | 1.1 | 3.1 | -2.0 | -2.4 | -2.6 | 1.7 | 1.3 | 2.2 |
Peroxisomes | -2.6 | -1.0 | -3.3 | -3.4 | -3.5 | -5.7 | -3.1 | -4.9 | -5.0 | -4.6 | -4.5 | -3.6 | 0.8 | 1.4 | 2.4 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 1.8 | 1.8 | 0 | 0 | 0 | 0.8 | 1.0 | 2.2 | 4.2 | 6.2 | 4.3 | 5.5 | 5.5 | 5.0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 10.8535 | 10.9978 | 11.027 | 10.2913 | 9.3642 | 10.8189 | 8.129 | 9.8892 | 8.9739 | 7.7233 | 8.0372 | 9.1786 | 8.9965 | 9.6244 | 8.9294 | 6.9897 | 7.1277 | 8.7457 |
Actin | 0.0055 | 0.0004 | 0.0003 | 0.0001 | 0.0002 | 0.0005 | 0.0405 | 0.0025 | 0.0028 | 0.0137 | 0.0087 | 0.0015 | 0.0145 | 0.0044 | 0.0081 | 0.0041 | 0.0688 | 0.0142 |
Bud | 0.0001 | 0.0001 | 0 | 0 | 0 | 0 | 0.0005 | 0.0014 | 0.0001 | 0.0002 | 0.0007 | 0 | 0.0011 | 0.0002 | 0 | 0.0001 | 0.0002 | 0 |
Bud Neck | 0.0004 | 0 | 0 | 0 | 0 | 0 | 0.0066 | 0.0001 | 0.0003 | 0.0001 | 0.0115 | 0 | 0.0006 | 0 | 0.0001 | 0.0001 | 0.0002 | 0.0001 |
Bud Periphery | 0.0002 | 0.0002 | 0.0001 | 0 | 0 | 0 | 0.0009 | 0.0022 | 0.0001 | 0.0003 | 0.0008 | 0.0001 | 0.0025 | 0.0004 | 0 | 0.0001 | 0.0003 | 0.0001 |
Bud Site | 0.0013 | 0.0002 | 0 | 0 | 0 | 0 | 0.0086 | 0.0022 | 0.0035 | 0.0006 | 0.0206 | 0 | 0.0026 | 0.0003 | 0.0003 | 0.0006 | 0.0008 | 0 |
Cell Periphery | 0.0001 | 0.0001 | 0 | 0 | 0 | 0 | 0.0006 | 0.0003 | 0.0001 | 0.0001 | 0.0003 | 0 | 0.0003 | 0.0001 | 0 | 0 | 0.0001 | 0 |
Cytoplasm | 0 | 0 | 0 | 0 | 0 | 0 | 0.0024 | 0.0001 | 0.0002 | 0.0058 | 0.0004 | 0 | 0.0018 | 0 | 0.0003 | 0.0002 | 0 | 0 |
Cytoplasmic Foci | 0.0013 | 0.0019 | 0.0001 | 0 | 0.0003 | 0.0005 | 0.0085 | 0.0017 | 0.0106 | 0.0067 | 0.0114 | 0.0007 | 0.0129 | 0.0005 | 0.0047 | 0.0126 | 0.001 | 0.0003 |
Eisosomes | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0009 | 0.0002 | 0.0001 | 0.0003 | 0.0003 | 0.0001 | 0.0002 | 0.0002 | 0.0001 | 0.0002 | 0.0005 | 0.0002 |
Endoplasmic Reticulum | 0 | 0 | 0.0001 | 0 | 0 | 0 | 0.008 | 0.0001 | 0.0001 | 0.0023 | 0.0003 | 0 | 0.0014 | 0 | 0.0001 | 0.0001 | 0.0001 | 0.0004 |
Endosome | 0.0032 | 0.0034 | 0.0022 | 0.0002 | 0.0014 | 0.0014 | 0.0206 | 0.013 | 0.0052 | 0.0252 | 0.0072 | 0.0024 | 0.0098 | 0.0016 | 0.0021 | 0.0039 | 0.0011 | 0.0012 |
Golgi | 0.01 | 0.0055 | 0.0016 | 0.0003 | 0.0212 | 0.0095 | 0.014 | 0.0241 | 0.0074 | 0.0065 | 0.0072 | 0.005 | 0.0051 | 0.0112 | 0.0019 | 0.004 | 0.0044 | 0.0033 |
Lipid Particles | 0.0053 | 0.0017 | 0.0009 | 0.0044 | 0.0003 | 0.0024 | 0.0193 | 0.0088 | 0.0178 | 0.036 | 0.0068 | 0.0083 | 0.0116 | 0.0064 | 0.004 | 0.0174 | 0.0067 | 0.0055 |
Mitochondria | 0.9601 | 0.9798 | 0.985 | 0.9931 | 0.9746 | 0.9835 | 0.7677 | 0.8945 | 0.9409 | 0.8877 | 0.9052 | 0.9678 | 0.9005 | 0.9675 | 0.9652 | 0.8217 | 0.9117 | 0.967 |
None | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0.0012 | 0 | 0 | 0.0008 | 0.0004 | 0 | 0.0013 | 0 | 0.0004 | 0.0005 | 0.0001 | 0.0001 |
Nuclear Periphery | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0.0317 | 0.001 | 0 | 0.0029 | 0.0004 | 0.0001 | 0.003 | 0 | 0.0002 | 0.0008 | 0.0001 | 0.0006 |
Nucleolus | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0.0037 | 0.0004 | 0.0001 | 0.0004 | 0.0019 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0.0008 | 0.0001 | 0.0001 |
Nucleus | 0.0001 | 0 | 0.0057 | 0 | 0 | 0 | 0.0076 | 0.0002 | 0 | 0.0008 | 0.0011 | 0 | 0.0007 | 0 | 0.0002 | 0.0005 | 0 | 0 |
Peroxisomes | 0.0099 | 0.0039 | 0.0011 | 0.0008 | 0.0011 | 0.0011 | 0.0248 | 0.0218 | 0.008 | 0.005 | 0.0071 | 0.012 | 0.0201 | 0.0046 | 0.0046 | 0.0524 | 0.0027 | 0.0043 |
Punctate Nuclear | 0 | 0 | 0 | 0 | 0 | 0 | 0.005 | 0 | 0.0003 | 0.0012 | 0.0063 | 0 | 0.0047 | 0 | 0.007 | 0.0792 | 0.0001 | 0.0001 |
Vacuole | 0.0005 | 0.0005 | 0.0007 | 0.0002 | 0.0001 | 0.0001 | 0.0067 | 0.0096 | 0.0006 | 0.002 | 0.0009 | 0.0003 | 0.0024 | 0.0002 | 0.0001 | 0.0001 | 0.0002 | 0.0002 |
Vacuole Periphery | 0.0014 | 0.002 | 0.0019 | 0.0007 | 0.0006 | 0.0006 | 0.0199 | 0.0157 | 0.0016 | 0.0015 | 0.0008 | 0.0013 | 0.0028 | 0.0022 | 0.0004 | 0.0006 | 0.0007 | 0.0022 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 15.9528 | 19.8068 | 18.8128 | 19.9457 | 21.8326 | 14.1724 | 15.0199 | 16.2199 | 22.86 | 18.4233 |
Translational Efficiency | 1.136 | 0.9449 | 1.0239 | 1.2047 | 1.0595 | 1.1853 | 1.1694 | 1.0184 | 0.7382 | 1.0486 |
Dataset | Hit |
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Protein Concentration | ✔ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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