Standard name
Human Ortholog
Description Protein of unknown function; required for proliferation or remodeling of the ER that is caused by overexpression of Hmg2p; may interact with ribosomes, based on co-purification experiments; HER1 has a paralog, GIP3, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0.05 0.05 0.05 0 0 0 0 0 0
Cytoplasm 0.98 0.97 0.98 0.98 0.95 0.89 0.86 0.79 0.75 – 0.66 0.64 1.0 0.97 0.98 0.88 0.93 0.92 0.91 0.86 0.82 0.9 0.88 0.84
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0.07 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0.08 0 0.13 0.12 0.37 0.36 0.41 0.51 – 0.6 0.6 0 0 0.06 0 0 0 0 0 0.09 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 1 0 – 0 0 0 1 0 0 0 0 1 0 2 1 0 4
Bud 0 0 3 3 3 5 1 11 13 – 13 14 2 1 7 0 0 0 1 0 1 2 2 6
Bud Neck 0 0 3 0 1 0 0 0 0 – 0 0 0 0 0 0 0 0 1 0 1 0 0 5
Bud Site 0 0 1 0 0 0 0 0 0 – 1 2 0 0 0 0 0 0 – – – – – –
Cell Periphery 10 1 4 3 1 2 8 2 12 – 12 15 2 0 3 11 9 9 1 0 1 0 0 1
Cytoplasm 321 100 321 318 319 271 349 311 294 – 214 278 234 294 329 179 184 151 318 103 291 144 211 243
Endoplasmic Reticulum 2 0 1 0 0 0 0 0 1 – 3 1 0 2 1 6 13 6 1 0 0 3 1 3
Endosome 2 0 0 1 4 0 1 0 0 – 0 0 0 0 0 6 1 0 3 1 2 1 0 3
Golgi 0 0 1 0 0 0 0 0 0 – 0 0 0 0 0 5 1 2 1 0 2 0 0 3
Mitochondria 1 8 11 42 41 112 145 162 197 – 195 261 4 6 20 8 3 2 10 4 30 1 8 6
Nucleus 0 0 0 1 1 1 1 1 2 – 0 0 0 2 0 7 2 3 1 1 0 0 1 0
Nuclear Periphery 1 0 0 0 0 0 0 1 0 – 2 1 0 1 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 – 1 0 0 1 0 5 1 0 0 1 0 0 0 0
Peroxisomes 0 0 0 0 0 0 1 0 0 – 0 0 0 0 0 1 1 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 1 1 0 2 – 1 1 0 0 0 1 1 0 1 0 0 1 1 1
Vac/Vac Membrane 2 0 1 1 0 2 3 7 5 – 4 10 1 0 0 8 6 5 0 2 11 1 3 4
Unique Cell Count 327 103 327 326 335 303 405 395 390 323 433 235 304 335 204 197 165 351 120 354 161 240 290
Labelled Cell Count 339 109 346 369 370 394 510 496 526 446 583 243 308 360 237 222 178 351 120 354 161 240 290


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.1 4.3 3.6 3.5 4.1 4.0 3.9 4.2 3.8 – 3.8 3.7 4.5 4.9 4.5 7.3 7.8 7.0 4.8 4.5 5.4
Std Deviation (1e-4) 0.6 1.0 0.8 1.0 1.3 1.4 1.1 1.3 1.2 – 1.0 1.0 1.4 1.3 1.3 2.8 3.6 2.2 1.8 1.6 1.8
Intensity Change (Log2) – – – -0.05 0.19 0.13 0.1 0.22 0.06 – 0.05 0.04 0.3 0.42 0.3 1.0 1.1 0.94 0.4 0.31 0.56


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.194 3.0428 2.931 3.1929 2.9637 3.1521 2.3384 2.3428 1.9444 2.2065 1.4247 1.9339 2.9201 2.5609 2.8815 2.9028 2.4279 2.6768
Actin 0.0003 0.0002 0.0002 0.0008 0.0001 0.0003 0.0323 0.0016 0.0166 0.0032 0.0016 0.0095 0.0023 0.0086 0.0244 0.0281 0.0002 0.0051
Bud 0 0.0001 0.0002 0.0003 0 0.0001 0.001 0.0034 0.0064 0.0002 0.0002 0.0027 0.0009 0.0005 0.0017 0.0031 0 0.0019
Bud Neck 0.0001 0.0001 0.001 0.0005 0.0002 0.0018 0.0052 0.0008 0.0014 0.0001 0.0002 0.0069 0.0003 0.0001 0.0004 0.0006 0.0001 0.0008
Bud Periphery 0.0001 0.0003 0.0005 0.0005 0 0.0004 0.0023 0.0007 0.0145 0.0001 0.0002 0.0064 0.0022 0.0007 0.0036 0.0035 0 0.0037
Bud Site 0.0001 0.0009 0.0041 0.0006 0 0.0001 0.0188 0.0171 0.0261 0.001 0.0003 0.0014 0.003 0.0037 0.0035 0.0054 0 0.0002
Cell Periphery 0.0007 0.0028 0.0038 0.0007 0.0002 0.0011 0.0012 0.0005 0.001 0.0001 0.0001 0.0004 0.0006 0.0003 0.0004 0.0004 0.0001 0.0002
Cytoplasm 0.5132 0.7929 0.7627 0.7717 0.7933 0.8471 0.2366 0.438 0.3448 0.4097 0.4715 0.5643 0.2319 0.4249 0.2928 0.3678 0.1941 0.3884
Cytoplasmic Foci 0.0037 0.0025 0.0028 0.0046 0.0031 0.0039 0.044 0.0161 0.0116 0.0259 0.0054 0.01 0.0174 0.004 0.0155 0.0213 0.0026 0.0117
Eisosomes 0.0001 0.0001 0.0001 0.0001 0 0 0.0003 0.0001 0.0002 0 0.0001 0.0002 0.0002 0.0003 0.0002 0.0001 0.0001 0.0001
Endoplasmic Reticulum 0.0038 0.003 0.0034 0.0047 0.0036 0.0021 0.0068 0.001 0.0098 0.0011 0.0017 0.0017 0.0065 0.0008 0.0019 0.0065 0.0008 0.0016
Endosome 0.0012 0.0008 0.0011 0.0024 0.0028 0.0026 0.041 0.0011 0.0069 0.0079 0.0134 0.0105 0.042 0.0015 0.0098 0.0219 0.0003 0.01
Golgi 0.0002 0.0001 0.0001 0.0003 0.0001 0.0001 0.0119 0.0003 0.0025 0.0037 0.0006 0.0024 0.017 0.0018 0.0149 0.0177 0.0001 0.0035
Lipid Particles 0.0003 0.0002 0.0002 0.0004 0 0.0001 0.0427 0.0002 0.0006 0.0049 0.0012 0.0016 0.0053 0.0002 0.0145 0.0027 0.0001 0.0009
Mitochondria 0.0006 0.0001 0.0001 0.0002 0.0001 0.0001 0.0174 0.0026 0.012 0.0004 0.0009 0.0078 0.0054 0.0147 0.004 0.0053 0.0001 0.0094
None 0.4728 0.1933 0.2171 0.2081 0.1947 0.1391 0.4449 0.5131 0.5304 0.5327 0.4378 0.3656 0.6246 0.5358 0.6005 0.5003 0.8006 0.5511
Nuclear Periphery 0.0003 0.0003 0.0003 0.0006 0.0003 0.0001 0.0255 0.0002 0.0007 0.0003 0.0344 0.0009 0.0098 0.0002 0.0005 0.0014 0.0001 0.0028
Nucleolus 0 0.0001 0.0001 0.0001 0 0 0.0011 0.0007 0.0003 0 0.0009 0.0003 0.0007 0.0001 0.0002 0.0001 0 0.0004
Nucleus 0.0004 0.0005 0.0006 0.0008 0.0004 0.0003 0.0109 0.0008 0.0008 0.0003 0.0086 0.0009 0.0142 0.0004 0.0006 0.0008 0.0001 0.0019
Peroxisomes 0 0 0 0.0001 0 0 0.0228 0.0005 0.0014 0.0069 0.0006 0.0023 0.0015 0.0009 0.009 0.0098 0 0.0006
Punctate Nuclear 0.0001 0.0001 0.0001 0.0003 0.0001 0.0001 0.0283 0.0005 0.002 0.0007 0.0181 0.0014 0.0019 0.0001 0.0009 0.0013 0.0001 0.0042
Vacuole 0.0018 0.0016 0.0016 0.0021 0.0009 0.0006 0.0033 0.0008 0.0086 0.0006 0.0012 0.0021 0.0098 0.0005 0.0007 0.0013 0.0002 0.0011
Vacuole Periphery 0.0001 0.0001 0.0001 0.0002 0.0001 0.0001 0.0016 0.0001 0.0016 0.0001 0.0011 0.0006 0.0024 0.0001 0.0002 0.0005 0 0.0004

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 25.9555 14.7653 17.413 28.115 18.1647 21.8141 25.5263 27.3497 29.1162 28.0219
Translational Efficiency 0.4514 0.6005 0.474 0.3821 0.5223 0.8319 0.422 0.512 0.482 0.4404

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
2108 98 162 1357 2147 1505 1016 116 4255 1603 1178 1473

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 652.62 764.09 1161.34 851.49 675.59 750.26 888.78 901.06 664.21 751.11 926.26 855.39
Standard Deviation 71.54 90.48 129.52 108.97 95.60 97.22 101.48 113.64 85.31 96.88 141.42 110.16
Intensity Change Log 2 — 0.227499 0.831475 0.383746 — 0.151243 0.395678 0.415475 — 0.189215 0.626225 0.399972

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000064 0.000462 0.000747 0.000710 0.000164 0.000316 0.000124 0.000165 0.000115 0.000325 0.000210 0.000667
Bud Neck 0.006513 0.016036 0.001607 0.005023 0.019414 0.036339 0.001447 0.002526 0.013022 0.035098 0.001469 0.004827
Bud Site 0.003914 0.018798 0.014242 0.033153 0.004937 0.024710 0.004285 0.006912 0.004430 0.024348 0.005654 0.031087
Cell Periphery 0.000145 0.000122 0.000331 0.000147 0.000111 0.000163 0.000141 0.000106 0.000128 0.000160 0.000167 0.000143
Cytoplasm 0.662118* 0.352813* 0.493669* 0.529380* 0.513040* 0.469045* 0.580906* 0.446517* 0.586896* 0.461939* 0.568909* 0.522854*
Cytoplasmic Foci 0.123714 0.358882* 0.056374 0.034808 0.230475* 0.221849* 0.023705 0.039758 0.177584 0.230227* 0.028198 0.035198
Eisosomes 0.000119 0.000171 0.000083 0.000054 0.000203 0.000153 0.000051 0.000070 0.000161 0.000154 0.000055 0.000056
Endoplasmic Reticulum 0.000249 0.000890 0.003269 0.001169 0.001173 0.000542 0.001546 0.002496 0.000715 0.000563 0.001783 0.001273
Endosome 0.003035 0.039569 0.015408 0.012154 0.012693 0.033921 0.003158 0.022242 0.007908 0.034266 0.004843 0.012949
Golgi 0.000715 0.005394 0.000307 0.005365 0.003609 0.006508 0.000071 0.008094 0.002176 0.006440 0.000103 0.005580
Lipid Particles 0.002307 0.009436 0.003542 0.001058 0.008564 0.005728 0.001315 0.001450 0.005464 0.005955 0.001622 0.001089
Mitochondria 0.000616 0.010839 0.000086 0.007680 0.003436 0.010633 0.000203 0.000418 0.002039 0.010646 0.000187 0.007109
Mitotic Spindle 0.000351 0.001130 0.003769 0.041377 0.002150 0.010156 0.002554 0.004562 0.001259 0.009604 0.002721 0.038478
None 0.007861 0.001258 0.000833 0.001509 0.002330 0.002020 0.004066 0.001419 0.005070 0.001973 0.003621 0.001502
Nuclear Periphery 0.000121 0.000376 0.000827 0.000896 0.000394 0.000249 0.000968 0.001052 0.000258 0.000257 0.000949 0.000908
Nuclear Periphery Foci 0.000210 0.001016 0.014702 0.001112 0.000299 0.000239 0.001572 0.001975 0.000255 0.000286 0.003377 0.001180
Nucleolus 0.000734 0.000917 0.000227 0.000229 0.001085 0.001369 0.000235 0.000125 0.000911 0.001341 0.000234 0.000221
Nucleus 0.151927* 0.044140 0.057843 0.095455 0.122920 0.058124 0.255867* 0.154026* 0.137291* 0.057269 0.228635* 0.100067
Peroxisomes 0.001137 0.033162 0.000382 0.002642 0.005922 0.010162 0.000655 0.000293 0.003551 0.011568 0.000617 0.002457
Vacuole 0.033907 0.102190 0.331353* 0.223147* 0.066049 0.105182 0.117011 0.302606* 0.050125 0.104999 0.146487 0.229405*
Vacuole Periphery 0.000244 0.002401 0.000396 0.002933 0.001033 0.002594 0.000121 0.003187 0.000642 0.002582 0.000159 0.002953

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -3.14 -3.66 -4.17 0.22 1.22 -2.90 -0.96 -2.64 0.94 -2.09 -4.35 -4.88 -3.71 -0.89 -1.93
Bud Neck -3.42 7.30 3.23 4.38 -6.84 -7.30 16.93 13.99 15.54 -2.11 -10.78 17.77 13.04 15.72 -6.60
Bud Site -2.89 -2.47 -7.02 -0.08 -1.89 -9.30 1.10 -1.18 6.28 -1.60 -9.88 -0.71 -7.03 2.48 -6.33
Cell Periphery 1.42 -2.82 3.10 1.72 4.09 -3.17 -2.95 0.53 3.03 2.26 -1.90 -2.15 3.18 3.85 5.28
Cytoplasm 11.60 12.31 25.19 -2.90 -1.63 4.69 3.81 7.67 5.91 5.94 15.46 13.79 19.25 3.81 3.53
Cytoplasmic Foci -9.86 15.05 30.87 14.31 4.80 1.33 45.93 35.33 30.34 -2.21 -8.77 50.67 50.66 38.75 -0.52
Eisosomes -2.17 3.46 8.83 5.26 4.93 3.54 10.85 8.81 7.27 -2.17 0.87 12.28 14.08 12.93 3.64
Endoplasmic Reticulum -1.34 -7.88 -12.47 -0.87 5.37 2.50 -4.82 -3.35 -5.46 -0.39 1.00 -10.62 -4.89 -6.94 7.92
Endosome -4.45 -7.30 -2.82 4.15 4.92 -10.73 12.84 1.66 6.70 -1.00 -14.37 7.49 2.96 14.83 -1.31
Golgi -2.84 5.01 -1.91 2.02 -2.75 -3.95 11.30 1.27 2.90 -1.01 -6.57 12.59 0.69 5.62 -3.20
Lipid Particles -1.54 -1.92 4.65 1.82 5.44 3.22 11.11 10.27 5.76 -1.41 -0.56 10.41 12.39 7.15 5.91
Mitochondria -2.22 13.43 -2.34 1.70 -2.92 -4.82 10.70 10.54 7.16 0.21 -6.12 11.86 -0.62 4.90 -2.78
Mitotic Spindle -1.92 -1.17 -7.67 -7.33 -3.85 -4.14 -1.54 -1.79 -0.94 -1.50 -4.88 -2.40 -7.50 -3.78 -5.56
None 7.24 9.78 7.80 -1.26 -7.21 0.55 -2.98 -0.46 -0.73 0.96 5.35 3.99 7.07 -0.17 3.41
Nuclear Periphery -1.51 -11.52 -19.02 -4.64 -3.48 2.19 -14.24 -7.10 -8.58 0.44 -0.07 -21.90 -16.50 -15.97 0.18
Nuclear Periphery Foci -1.24 -12.48 -8.35 -0.04 11.79 1.63 -11.49 -6.35 -6.59 -0.85 -0.55 -14.25 -9.13 -8.06 10.19
Nucleolus -0.63 3.00 2.86 2.50 -0.93 -0.90 6.05 6.54 4.97 2.51 -1.58 6.04 6.04 4.88 -0.11
Nucleus 7.65 15.40 6.95 -4.90 -8.95 17.93 -19.79 -0.90 -4.81 8.11 26.01 -15.97 4.11 -14.05 16.88
Peroxisomes -4.49 4.46 0.37 4.51 -1.70 -4.25 9.25 10.09 11.76 0.53 -8.36 9.07 5.48 10.82 -1.45
Vacuole -5.64 -18.70 -39.43 -16.26 3.43 -9.49 -16.86 -13.57 -12.15 -8.98 -15.00 -23.75 -39.71 -30.17 -12.95
Vacuole Periphery -2.83 -1.77 -2.74 1.57 -2.46 -5.87 9.98 0.59 2.95 -0.97 -7.97 10.34 -1.38 4.19 -3.22
Standard name
Human Ortholog
Description Protein of unknown function; required for proliferation or remodeling of the ER that is caused by overexpression of Hmg2p; may interact with ribosomes, based on co-purification experiments; HER1 has a paralog, GIP3, that arose from the whole genome duplication
Localization
Cell Percentages cytoplasm (45%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Her1

Her1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Her1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available