ORF | |
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Human Ortholog | |
Description | Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 3 | 1 | 2 | 0 | 1 |
Bud | 0 | 2 | 0 | 0 | 0 | 1 | 0 | 1 | 1 | 3 | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 0 | 0 | 3 | 4 |
Bud Neck | 0 | 1 | 2 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 2 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 1 | 0 | 2 | 0 | 0 | 3 | 0 | 2 | 0 | 1 | 0 | 0 | 1 | 2 | 0 | 1 | 9 | 4 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 267 | 451 | 100 | 118 | 78 | 119 | 141 | 189 | 70 | 68 | 48 | 39 | 290 | 394 | 624 | 231 | 274 | 267 | 256 | 445 | 109 | 230 | 312 | 317 |
Endoplasmic Reticulum | 2 | 3 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 2 | 2 | 12 | 14 | 13 | 0 | 0 | 1 | 2 | 1 | 4 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 2 | 1 | 1 | 0 | 0 | 2 | 4 | 0 | 0 | 1 | 4 |
Golgi | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 2 | 1 | 1 | 0 | 1 | 3 |
Mitochondria | 0 | 13 | 0 | 0 | 4 | 13 | 21 | 32 | 38 | 44 | 38 | 31 | 0 | 0 | 0 | 4 | 5 | 3 | 2 | 11 | 3 | 1 | 1 | 1 |
Nucleus | 1 | 2 | 2 | 2 | 5 | 3 | 4 | 1 | 0 | 2 | 1 | 2 | 0 | 0 | 1 | 1 | 3 | 1 | 1 | 1 | 0 | 0 | 1 | 1 |
Nuclear Periphery | 1 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 2 | 1 | 1 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 2 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 2 | 0 | 1 | 1 | 1 |
Vac/Vac Membrane | 2 | 3 | 0 | 0 | 0 | 0 | 6 | 18 | 4 | 2 | 3 | 4 | 3 | 3 | 3 | 3 | 8 | 6 | 5 | 5 | 0 | 1 | 0 | 3 |
Unique Cell Count | 271 | 468 | 103 | 118 | 84 | 129 | 161 | 223 | 94 | 98 | 74 | 63 | 291 | 396 | 629 | 236 | 281 | 270 | 281 | 487 | 122 | 252 | 332 | 359 |
Labelled Cell Count | 274 | 477 | 107 | 120 | 88 | 139 | 178 | 244 | 115 | 120 | 94 | 79 | 294 | 403 | 631 | 255 | 315 | 295 | 281 | 487 | 122 | 252 | 332 | 359 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 6.6 | 4.7 | 6.5 | 5.7 | 4.7 | 5.3 | 5.1 | 5.5 | 4.5 | 4.6 | 4.8 | 5.9 | 6.4 | 6.7 | 6.0 | 5.6 | 6.8 | 6.8 | 6.9 | 7.3 | 7.9 |
Std Deviation (1e-4) | 1.0 | 0.8 | 1.8 | 0.7 | 0.9 | 1.1 | 0.9 | 1.1 | 1.2 | 1.2 | 1.9 | 5.3 | 1.1 | 1.1 | 1.0 | 1.3 | 1.7 | 1.5 | 1.8 | 1.4 | 1.8 |
Intensity Change (Log2) | – | – | – | -0.2 | -0.46 | -0.3 | -0.34 | -0.25 | -0.52 | -0.49 | -0.45 | -0.15 | -0.03 | 0.04 | -0.11 | -0.21 | 0.06 | 0.06 | 0.08 | 0.17 | 0.29 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 3.5957 | 4.1509 | 3.9208 | 3.3381 | 3.7436 | 4.3518 | 5.9561 | 6.5677 | 6.7101 | 5.8942 | 5.7244 | 6.3316 | 3.5492 | 3.4494 | 3.4236 | 3.0337 | 2.6562 | 3.3532 |
Actin | 0.0339 | 0.0008 | 0.0222 | 0.0068 | 0.0053 | 0.0142 | 0.0676 | 0.0007 | 0.0265 | 0.0075 | 0.0137 | 0.002 | 0.0289 | 0.0055 | 0.0232 | 0.0282 | 0.0811 | 0.0113 |
Bud | 0.0002 | 0.0094 | 0.0004 | 0.0012 | 0.0003 | 0.0058 | 0.0052 | 0.0003 | 0.0034 | 0.0149 | 0.0049 | 0.0002 | 0.0032 | 0.0002 | 0.0024 | 0.0002 | 0.0018 | 0.0007 |
Bud Neck | 0.0003 | 0.0001 | 0.0007 | 0.0026 | 0.001 | 0.0036 | 0.0013 | 0.0001 | 0.001 | 0.0006 | 0.0014 | 0.0006 | 0.0064 | 0.0002 | 0.0005 | 0.0073 | 0.004 | 0.0009 |
Bud Periphery | 0.0003 | 0.0029 | 0.0004 | 0.0016 | 0.0004 | 0.0027 | 0.0145 | 0.0004 | 0.0055 | 0.0412 | 0.0067 | 0.0002 | 0.007 | 0.0001 | 0.0036 | 0.0002 | 0.0023 | 0.0014 |
Bud Site | 0.002 | 0.0044 | 0.0023 | 0.0362 | 0.0007 | 0.0009 | 0.0051 | 0.0029 | 0.0024 | 0.0038 | 0.0151 | 0.0001 | 0.0175 | 0.0018 | 0.002 | 0.0013 | 0.0041 | 0.0002 |
Cell Periphery | 0.0002 | 0.0004 | 0.0003 | 0.0005 | 0.0002 | 0.0001 | 0.0004 | 0.0003 | 0.0016 | 0.0004 | 0.0006 | 0.0001 | 0.0006 | 0.0002 | 0.0002 | 0.0001 | 0.0002 | 0.0001 |
Cytoplasm | 0.4535 | 0.7594 | 0.694 | 0.7195 | 0.4646 | 0.6114 | 0.4176 | 0.7948 | 0.6651 | 0.6776 | 0.7003 | 0.729 | 0.2786 | 0.6737 | 0.5901 | 0.4691 | 0.5163 | 0.6863 |
Cytoplasmic Foci | 0.0266 | 0.0031 | 0.0096 | 0.0087 | 0.0231 | 0.0297 | 0.0259 | 0.0026 | 0.0134 | 0.0097 | 0.0142 | 0.011 | 0.0361 | 0.005 | 0.0174 | 0.0282 | 0.041 | 0.0057 |
Eisosomes | 0.0008 | 0 | 0.0003 | 0.0002 | 0.0001 | 0.0001 | 0.0005 | 0.0002 | 0.0022 | 0.0001 | 0.0002 | 0.0001 | 0.0004 | 0.0001 | 0.0003 | 0.0002 | 0.0003 | 0 |
Endoplasmic Reticulum | 0.0025 | 0.0015 | 0.0031 | 0.0023 | 0.0012 | 0.0036 | 0.0045 | 0.0019 | 0.0072 | 0.0024 | 0.0188 | 0.0031 | 0.0139 | 0.0017 | 0.0029 | 0.002 | 0.0024 | 0.0012 |
Endosome | 0.0139 | 0.001 | 0.012 | 0.0028 | 0.0904 | 0.0195 | 0.0487 | 0.0015 | 0.0108 | 0.0165 | 0.031 | 0.0034 | 0.0445 | 0.0018 | 0.0131 | 0.0038 | 0.013 | 0.0013 |
Golgi | 0.0081 | 0.0001 | 0.0081 | 0.0011 | 0.0756 | 0.0105 | 0.0283 | 0.0006 | 0.0087 | 0.002 | 0.0199 | 0.0008 | 0.0191 | 0.0034 | 0.0051 | 0.0106 | 0.0199 | 0.0006 |
Lipid Particles | 0.022 | 0 | 0.0036 | 0.0028 | 0.014 | 0.0105 | 0.0176 | 0.0002 | 0.0012 | 0.0243 | 0.0053 | 0.0006 | 0.0203 | 0.0002 | 0.0022 | 0.0135 | 0.0182 | 0.0002 |
Mitochondria | 0.0093 | 0.0002 | 0.0141 | 0.0028 | 0.0437 | 0.0065 | 0.0176 | 0.0253 | 0.0051 | 0.0039 | 0.0387 | 0.0044 | 0.0309 | 0.0011 | 0.0036 | 0.0018 | 0.0058 | 0.0002 |
None | 0.3823 | 0.2127 | 0.2199 | 0.1973 | 0.2583 | 0.2532 | 0.3139 | 0.1342 | 0.223 | 0.1754 | 0.1049 | 0.2343 | 0.4094 | 0.3013 | 0.3076 | 0.3574 | 0.2186 | 0.286 |
Nuclear Periphery | 0.003 | 0.0005 | 0.0008 | 0.001 | 0.0004 | 0.0054 | 0.0034 | 0.0005 | 0.01 | 0.0013 | 0.0053 | 0.001 | 0.0289 | 0.0005 | 0.0039 | 0.0003 | 0.0007 | 0.0003 |
Nucleolus | 0.0016 | 0.0001 | 0.0001 | 0.0005 | 0.0002 | 0.0002 | 0.0017 | 0.0215 | 0.0004 | 0.002 | 0.0013 | 0.0001 | 0.0034 | 0.0001 | 0.0003 | 0.0001 | 0.0002 | 0 |
Nucleus | 0.003 | 0.0022 | 0.0014 | 0.0044 | 0.0008 | 0.0032 | 0.0022 | 0.0101 | 0.005 | 0.0057 | 0.0031 | 0.0018 | 0.0129 | 0.0015 | 0.0058 | 0.0005 | 0.0008 | 0.0017 |
Peroxisomes | 0.0184 | 0.0001 | 0.0042 | 0.0051 | 0.0062 | 0.014 | 0.0081 | 0.0002 | 0.0024 | 0.0042 | 0.0042 | 0.0057 | 0.0145 | 0.0006 | 0.0104 | 0.0729 | 0.0661 | 0.0007 |
Punctate Nuclear | 0.0158 | 0.0005 | 0.0005 | 0.0014 | 0.0003 | 0.0025 | 0.0106 | 0.0009 | 0.0027 | 0.0015 | 0.0009 | 0.0008 | 0.0133 | 0.0004 | 0.0015 | 0.0018 | 0.0023 | 0.0006 |
Vacuole | 0.0016 | 0.0006 | 0.0015 | 0.0012 | 0.0081 | 0.0016 | 0.0035 | 0.0007 | 0.0016 | 0.0044 | 0.0066 | 0.0005 | 0.0059 | 0.0006 | 0.0026 | 0.0003 | 0.0006 | 0.0003 |
Vacuole Periphery | 0.0005 | 0.0001 | 0.0004 | 0.0002 | 0.0052 | 0.0008 | 0.0017 | 0.0002 | 0.0009 | 0.0006 | 0.0027 | 0.0003 | 0.0046 | 0.0001 | 0.0015 | 0.0001 | 0.0002 | 0.0001 |
R1 | R2 | |||||||||
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G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 16.3333 | 8.6524 | 10.4333 | 9.2935 | 14.1179 | 15.7196 | 9.842 | 11.0712 | 8.6686 | 14.9872 |
Translational Efficiency | 1.4349 | 1.5576 | 0.5024 | 0.5496 | 0.9442 | 1.255 | 0.9349 | 0.6534 | 0.7605 | 1.0358 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
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37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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