Standard name
Human Ortholog
Description Diacylglycerol acyltransferase; catalyzes the terminal step of triacylglycerol (TAG) formation, acylates diacylglycerol using acyl-CoA as an acyl donor; Lro1p and Dga1p can O-acylate ceramides; localized to lipid particles
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.07 0.07 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0.05 0 0 0 0.07 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0.08 0.05 0 0.06 0 0 0 0 0 0 0 0 0 0.08 0 0.09 0.13 0.08 0 0 0 0 0 0
Cytoplasm 0.59 0.57 0.58 0.68 0.75 0.65 0.52 0.52 0.42 0.4 0.39 0.39 0.77 0.73 0.69 0.55 0.66 0.58 0.14 0.33 0.07 0.5 0.54 0.52
Endoplasmic Reticulum 0.23 0.4 0.32 0.1 0.07 0.08 0 0 0.05 0.11 0 0.05 0.21 0.26 0.33 0 0 0 0.61 0.51 0.71 0.22 0.18 0.22
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0 0.05 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.15 0.05 0 0.21 0.13 0.34 0.51 0.38 0.53 0.54 0.61 0.59 0 0 0 0.05 0.05 0.06 0.11 0 0.11 0.11 0.11 0.07
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0.07 0.06 0.12 0.16 0.14 0.12 0.15 0.16 0 0 0 0.23 0.17 0.22 0.05 0.05 0 0 0 0.06
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 2 1 5 5 1 1 1 0 1 2 1 1 2 2 6 13 11 11 5 2 11 3 13 8
Bud 2 0 4 9 8 7 37 16 19 22 34 22 0 0 0 1 1 2 3 0 0 0 5 9
Bud Neck 0 0 1 0 0 1 0 0 0 0 0 0 0 2 0 0 0 0 2 5 4 4 7 10
Bud Site 0 0 0 0 0 0 1 3 0 3 6 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 11 5 8 17 11 13 12 9 10 10 4 7 4 31 14 22 21 14 2 0 6 4 15 16
Cytoplasm 85 58 140 208 329 259 366 405 237 240 187 205 179 288 272 131 111 95 31 43 24 94 263 280
Endoplasmic Reticulum 33 40 78 32 32 32 23 34 29 69 15 26 49 103 129 5 4 5 141 67 235 41 90 116
Endosome 1 0 5 7 18 4 23 22 5 5 6 6 2 2 1 19 2 9 1 0 3 2 5 7
Golgi 0 0 1 1 0 0 0 0 0 0 0 0 0 0 0 2 3 0 0 0 3 1 0 0
Mitochondria 21 5 10 63 56 134 360 296 298 324 292 312 0 1 0 12 8 10 25 5 37 21 55 36
Nucleus 1 0 0 0 0 2 1 9 7 7 6 7 0 1 1 5 1 4 0 0 0 0 1 5
Nuclear Periphery 2 1 0 0 0 1 5 7 1 6 4 11 0 0 0 0 1 0 2 0 0 0 0 1
Nucleolus 1 0 0 1 1 0 0 1 3 2 1 3 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 2 1 0 0 2 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 1 0 0 2 2 3 1 0 0 0 1 0 4 2 0 0 1 0 0 1 2 2
Vac/Vac Membrane 5 0 7 12 29 23 87 127 78 73 70 87 4 10 1 55 29 37 10 6 4 8 20 35
Unique Cell Count 144 101 241 305 439 396 710 776 559 604 479 531 231 396 392 239 167 165 234 134 333 188 490 540
Labelled Cell Count 164 110 260 357 486 479 918 934 689 763 627 687 241 440 428 267 192 187 234 134 333 188 490 540


Cytoplasm, Endoplasmic Reticulum

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.8 5.5 5.3 5.2 5.2 4.5 4.4 4.6 4.3 4.3 4.2 4.4 6.4 6.6 6.5 8.2 8.4 7.3 6.4 6.4 6.8
Std Deviation (1e-4) 0.6 0.9 1.0 1.7 1.6 1.2 1.3 1.3 1.0 0.7 1.0 0.8 0.7 0.8 0.8 1.4 1.5 1.4 1.3 1.1 1.6
Intensity Change (Log2) – – – -0.03 -0.02 -0.22 -0.28 -0.2 -0.31 -0.29 -0.35 -0.27 0.28 0.32 0.29 0.62 0.65 0.47 0.27 0.27 0.36


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 1.9 2.3 2.3
Bud 1.0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 1.3 -0.6 0 0 0 0 0 0 0 -1.1 2.3 0.2 2.7 3.6 2.3
Cytoplasm 2.4 4.5 1.9 -1.8 -1.6 -4.1 -4.8 -4.8 -5.0 4.5 3.8 2.9 -0.7 1.7 -0.1
Endoplasmic Reticulum -6.3 -8.5 0 0 0 0 0 0 0 -2.7 -1.7 0.1 -8.8 -7.4 -7.2
Endosome 0.2 1.4 0 0 0 0 0 0 0 0 0 0 3.0 0 1.8
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 5.6 3.6 0 0 0 0 0 0 0 0 0 0 0.5 0.3 0.9
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0.7 2.1 1.7 4.2 5.4 4.7 4.1 4.8 5.3 -0.8 -0.3 0 6.6 5.1 6.2

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.5583 3.5322 3.5665 2.5799 2.957 3.341 -0.5332 0.7011 0.138 -0.2034 -0.133 0.2943 0.6898 0.8763 0.5607 0.701 0.5462 0.4262
Actin 0.0005 0.0025 0.0046 0.0043 0.0181 0.0025 0.0139 0.0046 0.0093 0.0067 0.0044 0.0018 0.0212 0.0013 0.0143 0.0153 0.0325 0.0035
Bud 0.0003 0.0012 0.0004 0.0028 0.0003 0.0004 0.0002 0.0002 0.0001 0.0031 0.0006 0.0001 0.0034 0.0182 0.0028 0.0025 0.0063 0.0013
Bud Neck 0.0011 0.0012 0.0009 0.0018 0.0018 0.0043 0.0006 0.001 0.0005 0.0006 0.0016 0.0033 0.0047 0.0004 0.0015 0.0018 0.0018 0.0032
Bud Periphery 0.0005 0.0016 0.0005 0.0051 0.0006 0.0008 0.0003 0.0003 0.0001 0.0015 0.0005 0.0003 0.0017 0.0026 0.0012 0.0078 0.002 0.0021
Bud Site 0.004 0.0114 0.0057 0.0021 0.0005 0.0002 0.0016 0.0072 0.0011 0.0175 0.0009 0.0002 0.008 0.0091 0.003 0.0041 0.0023 0.0006
Cell Periphery 0.0026 0.0025 0.0028 0.0022 0.0009 0.002 0.0014 0.0027 0.001 0.0006 0.0006 0.0004 0.0012 0.0017 0.0013 0.0013 0.0005 0.0007
Cytoplasm 0.2911 0.402 0.4091 0.5186 0.4031 0.4777 0.3641 0.4385 0.4171 0.4047 0.5005 0.5514 0.3088 0.4637 0.4048 0.4897 0.3829 0.4815
Cytoplasmic Foci 0.0174 0.0353 0.0304 0.0269 0.0508 0.0273 0.0393 0.0388 0.031 0.0532 0.0272 0.0317 0.0495 0.0222 0.063 0.0302 0.0512 0.0274
Eisosomes 0.0001 0.0003 0.0003 0.0002 0.0003 0.0001 0.0003 0.0003 0.0001 0.0001 0.0001 0 0.0002 0.0001 0.0002 0.0003 0.0002 0.0001
Endoplasmic Reticulum 0.26 0.1228 0.2769 0.1668 0.1648 0.2405 0.2852 0.3009 0.3059 0.2567 0.0846 0.2426 0.2886 0.278 0.2336 0.2399 0.2031 0.2805
Endosome 0.0351 0.0891 0.0391 0.0151 0.037 0.0327 0.066 0.0428 0.0727 0.0594 0.1732 0.0565 0.0747 0.0428 0.0792 0.0386 0.0809 0.0309
Golgi 0.0018 0.0125 0.004 0.0022 0.0072 0.0042 0.0151 0.0032 0.0138 0.0409 0.0178 0.0096 0.0084 0.0022 0.0092 0.0059 0.0266 0.0033
Lipid Particles 0.0623 0.0121 0.0326 0.0052 0.05 0.0334 0.0525 0.0482 0.0207 0.0355 0.0415 0.0106 0.0504 0.0251 0.0297 0.0226 0.0338 0.0302
Mitochondria 0.0005 0.0013 0.0014 0.0016 0.0017 0.0023 0.0043 0.0004 0.0025 0.0175 0.0151 0.0279 0.0038 0.002 0.0046 0.0173 0.0086 0.0021
None 0.2857 0.2809 0.1654 0.1803 0.241 0.148 0.1154 0.0771 0.0954 0.0414 0.0907 0.0275 0.1152 0.0966 0.1098 0.0665 0.122 0.0963
Nuclear Periphery 0.0083 0.0058 0.0087 0.0134 0.0053 0.0098 0.0164 0.0129 0.0148 0.0113 0.0113 0.0182 0.0182 0.009 0.0179 0.0115 0.0117 0.0141
Nucleolus 0.0084 0.0007 0.0003 0.0038 0.0004 0.0002 0.0003 0.0007 0.0001 0.0001 0.001 0.0001 0.0048 0.0006 0.0012 0.0169 0.0005 0.0005
Nucleus 0.0044 0.0033 0.0017 0.0096 0.0034 0.004 0.0021 0.0033 0.0033 0.003 0.0033 0.0045 0.0055 0.0065 0.0053 0.0098 0.0061 0.0072
Peroxisomes 0.0004 0.0007 0.0027 0.0015 0.0041 0.0005 0.0083 0.0007 0.0007 0.0361 0.0014 0.0028 0.0056 0.0002 0.0013 0.0027 0.0088 0.0021
Punctate Nuclear 0.0027 0.0049 0.0034 0.0307 0.0029 0.002 0.0021 0.0043 0.0021 0.0024 0.0027 0.0009 0.0096 0.0028 0.0055 0.0038 0.0069 0.005
Vacuole 0.0125 0.0073 0.0084 0.0052 0.0053 0.0063 0.0089 0.0112 0.0067 0.0062 0.0148 0.0054 0.0137 0.0137 0.0092 0.0091 0.009 0.0065
Vacuole Periphery 0.0005 0.0007 0.0007 0.0009 0.0007 0.0008 0.0017 0.0007 0.001 0.0013 0.0062 0.0042 0.0029 0.0013 0.0014 0.0025 0.0024 0.001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 16.6783 15.6941 9.8989 16.6796 15.3646 20.1784 16.0667 18.2071 16.7717 21.1876
Translational Efficiency 1.1363 0.9483 1.1121 0.9157 1.0166 1.0409 0.9758 0.8627 1.1123 0.7612

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Diacylglycerol acyltransferase; catalyzes the terminal step of triacylglycerol (TAG) formation, acylates diacylglycerol using acyl-CoA as an acyl donor; Lro1p and Dga1p can O-acylate ceramides; localized to lipid particles
Localization
Cell Percentages ER (66%)
Cell Cycle Regulation No
Subcompartmental Group ER-3

Dga1

Dga1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Dga1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available