Standard name
Human Ortholog
Description DNA 5' AMP hydrolase involved in DNA repair; member of the histidine triad (HIT) superfamily of nucleotide-binding proteins; homolog of Aprataxin, a Hint related protein that is mutated in individuals with ataxia with oculomotor apraxia; relative distribution to nuclear foci decreases upon DNA replication stress
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.8 0.87 – 0.9 0.9 0.88 0.77 – 0.56 0.53 0.36 0.44 0.94 0.92 0.93 0.91 0.91 0.92 0.68 0.75 0.69 0.82 0.76 0.73
Endoplasmic Reticulum 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0.07 0 0.05 0 0 0 0 0 0
Endosome 0 0 – 0 0 0 0.05 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 – 0 0 0.24 0.11 – 0.59 0.41 0.56 0.5 0 0 0 0.05 0 0 0.05 0 0.06 0 0.07 0
Nucleus 0 0 – 0 0 0 0.07 – 0.09 0.1 0.15 0.21 0 0 0 0 0 0 0.08 0.07 0 0 0 0
Nuclear Periphery 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.19 0.12 – 0.07 0.1 0 0.08 – 0.05 0.05 0.05 0.06 0.08 0.08 0.07 0.07 0.09 0.07 0.1 0.09 0.08 0 0.05 0.05
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 2 0 1 0 2 1
Bud 0 0 – 0 0 2 1 – 1 1 3 2 0 0 0 0 0 0 1 0 1 0 4 3
Bud Neck 1 0 – 0 2 0 0 – 0 0 0 0 0 0 0 0 2 1 1 0 0 2 2 2
Bud Site 0 0 – 0 0 0 0 – 1 1 2 3 0 0 0 0 0 0 – – – – – –
Cell Periphery 3 1 – 0 1 0 1 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 180 198 – 54 111 77 147 – 58 77 51 67 180 218 226 184 147 172 169 178 38 120 170 156
Endoplasmic Reticulum 2 2 – 0 0 0 0 – 0 0 0 0 0 1 1 14 4 9 1 1 1 2 0 2
Endosome 6 0 – 0 1 0 9 – 0 2 0 1 0 2 2 3 1 1 5 2 0 0 1 5
Golgi 1 0 – 0 0 0 0 – 0 0 0 0 0 1 1 3 0 3 2 2 0 0 2 1
Mitochondria 3 7 – 0 3 21 21 – 61 59 80 76 1 0 1 10 5 6 12 5 3 6 16 9
Nucleus 7 4 – 1 1 0 14 – 9 15 22 31 3 1 1 1 2 1 20 17 0 1 1 5
Nuclear Periphery 1 0 – 0 0 1 0 – 1 0 0 1 0 0 0 0 0 0 0 0 0 1 0 1
Nucleolus 0 0 – 2 0 0 0 – 0 1 1 0 0 1 0 0 0 0 0 0 0 0 1 1
Peroxisomes 0 0 – 0 0 0 1 – 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 – 0 1 0 3 – 0 0 2 1 1 1 1 0 1 0 2 3 0 1 3 3
Vac/Vac Membrane 44 27 – 4 12 2 15 – 5 7 7 9 16 20 17 14 15 13 25 21 4 5 10 10
Unique Cell Count 226 227 60 124 88 191 104 145 142 151 192 237 243 202 161 187 249 239 55 147 225 214
Labelled Cell Count 248 239 61 132 103 212 136 163 168 191 201 245 251 229 177 206 249 239 55 147 225 214


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.4 4.2 – 5.2 5.5 4.0 4.5 – 3.8 4.3 3.8 4.2 5.0 5.1 5.3 5.2 5.7 5.2 4.3 4.6 5.2
Std Deviation (1e-4) 1.1 0.8 – 2.1 2.0 2.0 1.5 – 1.4 1.8 1.0 1.7 1.5 1.8 1.8 1.2 1.4 1.3 1.6 2.0 3.0
Intensity Change (Log2) – – – – – – – – – – – – – – – – – – – – –


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration – – – – – – – – – – – – – – – – – –
Actin 0.0254 0.0003 0.0069 0.005 0.0091 0.0014 0.089 0.0001 0.0307 0.0001 0.0001 0.0094 0.0005 0.0003 0.0005 0.0005 0.0004 0.0006
Bud 0.0023 0.0001 0.0061 0.0005 0.0002 0.0002 0.0008 0 0.0005 0 0 0.0019 0.0001 0.0005 0.0003 0.0005 0.0005 0.0001
Bud Neck 0.002 0.0003 0.0019 0.0031 0.0005 0.0022 0.0011 0.0001 0.0008 0.0002 0 0.0033 0.0003 0.0004 0.0004 0.0008 0.0014 0.002
Bud Periphery 0.001 0 0.0022 0.0005 0.0004 0.0004 0.0009 0 0.0004 0 0 0.002 0.0001 0.0003 0.0003 0.0005 0.0003 0.0001
Bud Site 0.01 0.0007 0.0051 0.0008 0.0007 0.0003 0.0175 0.0003 0.0025 0.0001 0 0.0011 0.0004 0.001 0.0008 0.0007 0.0018 0.0002
Cell Periphery 0.0004 0.0001 0.0002 0.0001 0 0.0001 0.0005 0.0001 0.0002 0 0 0.0001 0.0001 0.0001 0.0001 0.0001 0.0001 0
Cytoplasm 0.1495 0.2097 0.1953 0.1975 0.1973 0.255 0.2748 0.3785 0.413 0.281 0.2232 0.3902 0.2751 0.3819 0.2645 0.2602 0.3309 0.3269
Cytoplasmic Foci 0.0207 0.0117 0.0171 0.0385 0.0279 0.017 0.0596 0.003 0.0308 0.0037 0.0032 0.0147 0.0094 0.0053 0.0049 0.0137 0.0206 0.0054
Eisosomes 0.0003 0.0001 0.0001 0.0001 0.0002 0.0001 0.0005 0.0001 0.0002 0 0 0.0001 0.0001 0.0001 0.0002 0.0001 0 0.0001
Endoplasmic Reticulum 0.0022 0.0041 0.0047 0.0035 0.0016 0.0039 0.0112 0.0029 0.0069 0.0025 0.0027 0.0073 0.0056 0.0058 0.0039 0.0036 0.0029 0.0039
Endosome 0.0108 0.006 0.0158 0.0187 0.0071 0.0112 0.0682 0.0017 0.0147 0.0034 0.0012 0.0205 0.0034 0.0039 0.0043 0.012 0.0104 0.0034
Golgi 0.0039 0.0003 0.0053 0.018 0.0063 0.0016 0.0136 0 0.008 0 0 0.0133 0.0002 0.0002 0.0001 0.0002 0.0016 0.0001
Lipid Particles 0.0085 0.0023 0.0051 0.0122 0.0148 0.0064 0.0274 0.0002 0.033 0.0001 0.0001 0.0046 0.0007 0.0003 0.0006 0.0003 0.0003 0.0002
Mitochondria 0.009 0.0007 0.0106 0.0138 0.0166 0.0049 0.007 0.0002 0.0082 0.0004 0.0001 0.0237 0.0005 0.0006 0.0008 0.0008 0.0051 0.0006
None 0.5061 0.5007 0.436 0.4304 0.4713 0.3619 0.2898 0.5362 0.1606 0.4404 0.7657 0.3639 0.4966 0.3083 0.4658 0.3744 0.4498 0.3752
Nuclear Periphery 0.0066 0.0156 0.0215 0.0126 0.0079 0.0158 0.0243 0.0058 0.0274 0.0057 0.0012 0.0054 0.0084 0.0107 0.0095 0.0081 0.0045 0.0058
Nucleolus 0.0049 0.0025 0.003 0.0026 0.002 0.0029 0.0015 0.0004 0.0034 0.0014 0 0.0007 0.0012 0.0011 0.0015 0.0015 0.0016 0.0017
Nucleus 0.2141 0.2198 0.2171 0.1887 0.1504 0.2722 0.0238 0.0645 0.214 0.2549 0.0012 0.1158 0.1842 0.2678 0.2265 0.3001 0.1517 0.2537
Peroxisomes 0.0043 0.0004 0.0226 0.0096 0.0512 0.0012 0.0202 0 0.0257 0 0 0.009 0.0003 0.0001 0.0002 0.0003 0.0029 0.0001
Punctate Nuclear 0.0136 0.022 0.0185 0.0408 0.0334 0.0378 0.014 0.0028 0.0124 0.0046 0.0009 0.0102 0.0112 0.0094 0.0123 0.0195 0.0107 0.0183
Vacuole 0.0034 0.0023 0.0038 0.0022 0.0007 0.0025 0.0433 0.0028 0.0051 0.0012 0.0003 0.0022 0.0016 0.0018 0.0021 0.0018 0.002 0.0013
Vacuole Periphery 0.0009 0.0004 0.0009 0.0008 0.0003 0.001 0.0107 0.0003 0.0017 0.0002 0.0001 0.0007 0.0003 0.0003 0.0003 0.0003 0.0004 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 6.7487 3.0164 3.3575 5.9873 3.2812 1.6862 3.0499 6.1755 4.5667 6.9067
Translational Efficiency – – – – – – – – – –

Hit Data

Dataset Hit
Protein Concentration –
Protein Localization ✘
Gene Expression ✘
Translational Efficiency –

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
310 1328 138 1558 1507 1853 1433 2069 1817 3181 1571 3627

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 606.36 740.78 906.19 804.17 666.70 742.22 817.87 879.09 656.41 741.62 825.63 846.91
Standard Deviation 71.13 99.29 97.22 110.38 75.38 92.72 98.75 102.46 78.05 95.52 101.74 112.24
Intensity Change Log 2 — 0.288871 0.579639 0.407326 — 0.154809 0.294834 0.398973 — 0.220218 0.437510 0.402958

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000136 0.001090 0.000592 0.000547 0.000072 0.000373 0.000181 0.000311 0.000083 0.000672 0.000217 0.000412
Bud Neck 0.030885 0.037017 0.002015 0.008553 0.011114 0.041077 0.003394 0.008808 0.014487 0.039382 0.003273 0.008699
Bud Site 0.006458 0.018527 0.001261 0.027901 0.001771 0.022996 0.004615 0.017602 0.002570 0.021130 0.004320 0.022026
Cell Periphery 0.000507 0.000191 0.000153 0.000194 0.000141 0.000240 0.000125 0.000131 0.000203 0.000219 0.000128 0.000158
Cytoplasm 0.329175* 0.385704* 0.082634 0.354567* 0.415293* 0.440904* 0.130175* 0.270029* 0.400601* 0.417859* 0.125999* 0.306343*
Cytoplasmic Foci 0.304516* 0.222476* 0.002901 0.006899 0.169617 0.124577 0.002004 0.004847 0.192632* 0.165447 0.002083 0.005729
Eisosomes 0.000464 0.000121 0.000074 0.000033 0.000110 0.000107 0.000019 0.000025 0.000171 0.000113 0.000024 0.000029
Endoplasmic Reticulum 0.001203 0.001311 0.009604 0.002728 0.000516 0.000674 0.003113 0.003232 0.000633 0.000940 0.003683 0.003015
Endosome 0.009718 0.020853 0.001840 0.002560 0.007557 0.011051 0.000678 0.002510 0.007926 0.015143 0.000780 0.002532
Golgi 0.006392 0.006383 0.000065 0.001912 0.000954 0.002613 0.000033 0.002985 0.001882 0.004187 0.000036 0.002524
Lipid Particles 0.019258 0.006346 0.002083 0.000843 0.008104 0.005684 0.000498 0.000584 0.010007 0.005961 0.000637 0.000695
Mitochondria 0.038067 0.013017 0.000476 0.001795 0.004198 0.010078 0.000776 0.002354 0.009976 0.011305 0.000749 0.002114
Mitotic Spindle 0.000211 0.003598 0.000178 0.024110 0.001347 0.008310 0.002253 0.011038 0.001153 0.006343 0.002070 0.016654
None 0.004865 0.005717 0.002236 0.011015 0.006554 0.008209 0.006399 0.005547 0.006266 0.007168 0.006034 0.007896
Nuclear Periphery 0.000461 0.000532 0.002025 0.001853 0.000696 0.000513 0.001532 0.002183 0.000656 0.000521 0.001575 0.002041
Nuclear Periphery Foci 0.000178 0.000538 0.001071 0.000774 0.000492 0.000313 0.000261 0.000416 0.000438 0.000407 0.000332 0.000570
Nucleolus 0.001478 0.002372 0.000934 0.000968 0.001622 0.002932 0.000536 0.000442 0.001597 0.002698 0.000571 0.000668
Nucleus 0.204876* 0.185899* 0.848088* 0.497704* 0.338801* 0.258499* 0.830463* 0.624943* 0.315952* 0.228190* 0.832011* 0.570286*
Peroxisomes 0.012247 0.016917 0.000259 0.000818 0.004011 0.007995 0.000076 0.000262 0.005416 0.011720 0.000092 0.000501
Vacuole 0.025351 0.068557 0.041339 0.053067 0.026399 0.051220 0.012706 0.040416 0.026220 0.058458 0.015221 0.045850
Vacuole Periphery 0.003556 0.002833 0.000171 0.001158 0.000631 0.001637 0.000163 0.001335 0.001130 0.002136 0.000164 0.001259

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -2.09 -6.77 -3.54 1.37 2.92 -2.59 -8.83 -9.12 0.34 -2.75 -2.93 -10.61 -7.25 1.39 -1.90
Bud Neck -1.07 5.31 4.50 10.95 -3.96 -11.84 5.45 0.83 13.29 -7.18 -11.70 7.46 3.83 17.23 -7.45
Bud Site -5.77 3.06 -3.50 0.98 -6.14 -10.28 -2.50 -6.89 4.72 -3.95 -13.44 -1.65 -8.45 4.30 -5.74
Cell Periphery 4.09 3.99 4.73 3.14 2.54 -4.10 1.21 4.60 5.90 0.69 -0.41 3.59 5.54 6.64 0.29
Cytoplasm -3.49 13.53 2.88 9.57 -14.60 -2.86 36.56 22.85 26.43 -15.66 -2.18 38.32 20.51 25.83 -22.94
Cytoplasmic Foci 5.81 23.52 23.28 36.51 -4.44 7.46 35.38 34.86 31.01 -3.96 4.86 41.02 40.36 46.41 -6.80
Eisosomes 5.84 6.05 7.13 12.73 5.20 0.80 19.83 17.60 14.91 -6.10 5.10 12.60 11.91 19.54 -4.58
Endoplasmic Reticulum -0.47 -9.54 -10.57 -15.21 7.03 -2.24 -18.05 -19.98 -18.74 -0.07 -3.92 -19.23 -26.56 -24.21 2.81
Endosome -5.76 4.87 5.42 13.46 1.57 -3.18 9.11 7.44 11.20 -3.79 -7.22 10.51 8.84 17.40 -5.04
Golgi 0.10 5.08 4.16 5.38 -1.30 -4.61 7.68 -0.39 2.18 -2.04 -4.71 7.76 1.89 5.46 -2.47
Lipid Particles 5.19 6.77 7.50 10.28 4.06 3.10 12.78 12.24 9.92 -4.55 5.43 14.32 13.98 14.08 -3.58
Mitochondria 3.63 5.52 5.40 7.58 -4.45 -4.49 3.90 2.64 7.81 -4.25 -0.80 6.57 5.97 10.89 -4.50
Mitotic Spindle -4.71 -0.69 -7.51 -6.02 -7.45 -4.60 -0.61 -7.10 -2.60 -6.36 -5.35 -0.75 -9.96 -5.99 -8.89
None -0.36 2.51 -1.75 -1.57 -6.94 -1.18 0.90 0.86 2.39 -0.26 -0.75 1.08 -0.19 0.81 -1.78
Nuclear Periphery -1.22 -11.22 -13.25 -12.93 -4.41 2.78 -14.16 -21.68 -24.48 -12.55 2.30 -16.79 -22.70 -24.74 -12.47
Nuclear Periphery Foci -5.27 -5.81 -5.05 -3.82 -0.85 0.86 0.71 -1.61 -4.12 -4.26 0.28 0.05 -3.41 -5.45 -5.30
Nucleolus -2.75 1.99 1.64 5.61 -0.56 -3.34 6.35 3.93 5.50 -2.24 -4.17 6.79 4.26 7.39 -3.19
Nucleus 1.34 -27.01 -20.40 -32.82 14.76 8.94 -55.93 -31.37 -43.31 23.76 11.54 -62.86 -33.27 -53.24 34.02
Peroxisomes -2.43 7.89 7.69 12.75 -1.02 -4.86 10.39 9.90 10.71 -3.05 -7.88 12.89 12.03 16.53 -2.77
Vacuole -10.27 -4.83 -16.19 -8.91 -3.99 -9.28 2.12 -15.48 -7.28 -16.24 -15.15 -0.35 -23.48 -11.48 -21.66
Vacuole Periphery 0.70 3.11 2.74 6.06 -1.34 -3.84 6.23 0.10 2.98 -1.92 -3.63 4.97 2.14 6.06 -2.44
Standard name
Human Ortholog
Description DNA 5' AMP hydrolase involved in DNA repair; member of the histidine triad (HIT) superfamily of nucleotide-binding proteins; homolog of Aprataxin, a Hint related protein that is mutated in individuals with ataxia with oculomotor apraxia; relative distribution to nuclear foci decreases upon DNA replication stress
Localization
Cell Percentages —
Cell Cycle Regulation No
Subcompartmental Group N/A

Hnt3

Hnt3


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Hnt3-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available