Standard name
Human Ortholog
Description Subunit a of vacuolar-ATPase V0 domain; one of two isoforms (Vph1p and Stv1p); Vph1p is located in V-ATPase complexes of the vacuole while Stv1p is located in V-ATPase complexes of the Golgi and endosomes; relative distribution to the vacuolar membrane decreases upon DNA replication stress; human homolog ATP6V0A4 implicated in renal tubular acidosis, can complement yeast null mutant
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0.06 – 0.09 0.08 0.11 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.06 0 0 0 0 0.08 0.1 0.07 – 0.12 0.14 0.12 0 0 0.05 0.1 0.19 0.13 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0.06 0.13 0.05 0.06 0.08 – 0.05 0 0 0 0 0 0 0.07 0 0 0 0 0 0 0
Mitochondria 0.07 0.1 0.1 0.14 0.07 0.11 0.05 0 – 0.06 0.08 0.08 0 0 0 0 0 0 0 0 0.05 0.05 0 0
Nucleus 0.07 0.08 0 0 0 0.05 0 0 – 0 0 0 0.07 0.07 0.08 0.1 0.08 0.08 0.06 0.06 0 0.06 0.05 0
Nuclear Periphery 0 0 0 0 0 0.14 0.18 0.15 – 0.1 0.08 0.15 0 0 0 0.05 0 0.05 0 0.05 0.17 0.07 0.07 0.06
Nucleolus 0.11 0.12 0.1 0.16 0.08 0.09 0 0.05 – 0 0 0 0.1 0.1 0.1 0.08 0.05 0.09 0 0 0 0.07 0.06 0.07
Peroxisomes 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.83 0.82 0.91 0.85 0.82 0.6 0.64 0.59 – 0.59 0.62 0.55 0.86 0.85 0.83 0.72 0.71 0.78 0.81 0.79 0.69 0.69 0.73 0.71
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 4 2 16 – 14 6 10 0 0 0 0 0 0 0 0 0 0 0 1
Bud 0 1 0 0 0 0 0 0 – 1 2 0 0 0 0 0 0 0 0 1 6 1 4 4
Bud Neck 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 1 1 1 3 5 21 22 38 – 39 32 52 0 0 0 1 1 1 0 0 0 0 0 0
Cytoplasm 19 15 9 16 18 43 54 41 – 52 53 56 9 9 13 16 26 14 4 5 2 0 1 1
Endoplasmic Reticulum 1 0 1 0 2 9 12 24 – 18 10 16 1 1 0 8 2 3 1 2 6 0 2 1
Endosome 0 1 0 0 1 0 0 0 – 0 0 0 0 0 0 0 1 0 0 3 3 3 1 1
Golgi 7 1 20 22 55 28 33 45 – 21 11 18 3 1 3 5 10 3 2 1 2 4 6 12
Mitochondria 22 40 58 49 31 58 30 21 – 28 30 38 9 9 6 5 2 2 4 4 30 14 15 14
Nucleus 22 32 9 2 11 25 17 14 – 12 13 13 19 19 20 16 11 8 19 28 6 16 21 15
Nuclear Periphery 2 0 8 10 7 72 99 91 – 45 32 70 2 3 2 8 5 5 12 21 107 19 30 21
Nucleolus 36 50 57 57 34 49 24 31 – 11 10 13 28 28 24 12 7 10 14 16 20 19 24 25
Peroxisomes 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 1 – 0 0 0 0 0 0 0 0 0 0 1 0 1 0 0
Vac/Vac Membrane 268 338 510 304 351 314 358 356 – 263 232 259 243 238 199 110 95 83 274 378 434 192 316 257
Unique Cell Count 322 410 562 359 426 524 563 599 443 377 468 284 279 240 153 134 106 341 476 630 277 431 363
Labelled Cell Count 378 479 673 463 515 623 651 678 504 431 545 314 308 267 181 160 129 341 476 630 277 431 363


Vacuole Periphery

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 103.0 84.5 75.1 61.5 59.6 52.1 48.2 45.7 – 40.1 37.6 40.3 94.0 94.0 92.5 41.3 43.1 55.7 99.6 110.2 112.5
Std Deviation (1e-4) 28.6 31.4 24.1 22.4 21.5 19.7 18.7 16.7 – 15.1 13.8 14.1 28.0 28.0 30.4 20.7 23.7 28.7 36.5 36.7 41.4
Intensity Change (Log2) – – – -0.29 -0.33 -0.53 -0.64 -0.71 – -0.9 -1.0 -0.9 0.33 0.33 0.3 -0.86 -0.8 -0.43 0.41 0.55 0.58


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 3.9 – 4.2 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 4.5 4.4 5.8 – 6.9 6.8 7.9 0 0 0 0 0 0
Cytoplasm 2.6 2.5 5.1 5.8 4.4 – 6.7 7.5 6.8 1.5 1.5 3.0 5.3 8.5 0
Endoplasmic Reticulum 0 0 0 3.1 4.5 – 4.5 0 4.1 0 0 0 0 0 0
Endosome 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Golgi 1.8 5.5 1.4 1.8 2.9 – 0.9 -0.5 0.2 -2.1 -2.8 -1.8 -0.2 2.0 0
Mitochondria 1.5 -1.7 0.4 -3.1 -4.6 – -2.3 -1.2 -1.2 -3.6 -3.6 -3.7 -2.7 -3.3 -2.8
Nucleus 0 1.1 3.0 1.6 0.9 – 1.2 1.8 1.3 3.9 4.0 4.7 5.3 0 0
Nuclear Periphery 1.5 0.3 7.8 9.2 8.4 – 6.2 5.3 8.2 0 0 0 0 0 0
Nucleolus 2.6 -1.2 -0.4 -3.8 -3.2 – -4.8 -4.4 -4.7 -0.1 0 -0.1 -0.9 -1.8 -0.2
Peroxisomes 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Vacuole -2.8 -3.9 -11.9 -10.8 -12.2 – -11.7 -10.8 -13.0 -2.3 -2.4 -3.2 -6.1 -6.1 -3.7

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 81.3519 94.0299 93.4711 87.444 89.1845 85.2631 79.1848 93.9989 85.609 74.7839 88.1437 81.4704 91.8554 96.8194 94.7066 90.4827 87.2936 92.0248
Actin 0.0001 0 0 0 0 0 0.0052 0 0.0003 0.0212 0 0 0 0.0001 0 0 0 0
Bud 0 0 0.0001 0 0.0002 0 0.0002 0 0.0001 0.0003 0.0001 0.0001 0 0.0002 0 0 0.0002 0.0001
Bud Neck 0 0 0.0001 0 0 0 0.0019 0 0 0.0001 0 0 0 0.0003 0 0 0 0
Bud Periphery 0.0001 0 0.0002 0.0001 0.0013 0.0001 0.0003 0.0001 0.0001 0.0013 0.0003 0.0002 0 0.0004 0.0001 0.0002 0.0006 0.0002
Bud Site 0 0 0.0001 0 0 0 0.0012 0 0 0.0002 0.0001 0.0001 0 0.0002 0 0 0.0001 0
Cell Periphery 0.007 0.0048 0.008 0.0059 0.0117 0.0035 0.0097 0.0044 0.0066 0.0025 0.01 0.0048 0.0042 0.0054 0.0051 0.0102 0.0109 0.0035
Cytoplasm 0 0.0004 0.0001 0 0 0 0.0003 0 0.0002 0 0 0.0001 0 0.0014 0 0 0 0
Cytoplasmic Foci 0.0002 0 0 0 0 0 0.0069 0 0.0036 0.0005 0.0001 0.0004 0 0.0002 0 0 0 0
Eisosomes 0.0001 0 0 0 0 0 0.0001 0 0 0.0006 0 0 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0.0001 0 0.0001 0.0001 0 0 0 0 0 0 0 0
Endosome 0.0005 0.0003 0.0004 0.0003 0.0002 0.0003 0.0012 0.0003 0.0006 0.0011 0.0002 0.0007 0.0003 0.0004 0.0004 0.0003 0.0002 0.0002
Golgi 0 0 0 0 0 0 0.0008 0 0.0001 0.0034 0.0001 0.0001 0 0 0.0001 0 0 0
Lipid Particles 0.0014 0.0004 0.0017 0.0011 0.0013 0.0013 0.0064 0.0009 0.0025 0.005 0.0073 0.003 0.0015 0.0005 0.0025 0.0015 0.0015 0.0029
Mitochondria 0.0108 0.0015 0.0092 0.0043 0.0011 0.0017 0.017 0.0077 0.017 0.0418 0.0035 0.008 0.0142 0.003 0.0271 0.0012 0.0015 0.0163
None 0 0 0 0 0 0 0.0012 0 0.0003 0.0001 0 0 0 0.0002 0 0 0 0
Nuclear Periphery 0.0016 0.0075 0.0042 0.0028 0.0016 0.0149 0.0012 0.0022 0.0064 0.0024 0.0101 0.0063 0.0055 0.0107 0.0063 0.0006 0.0046 0.0081
Nucleolus 0.0264 0.0121 0.0264 0.0057 0.0234 0.007 0.0498 0.0089 0.0161 0.001 0.0448 0.0286 0.0101 0.0126 0.009 0.0104 0.019 0.0175
Nucleus 0.0044 0.0045 0.0062 0.0014 0.0024 0.0037 0.0056 0.0025 0.0046 0.0011 0.0044 0.0046 0.0022 0.0055 0.0021 0.002 0.0034 0.003
Peroxisomes 0.0001 0 0.0001 0.0001 0.0001 0 0.0006 0 0.0004 0.0029 0.0001 0.0002 0.0001 0.0001 0.0001 0 0.0001 0.0001
Punctate Nuclear 0 0 0 0 0 0 0.0028 0 0.0013 0.0001 0 0 0 0 0 0 0 0
Vacuole 0.1916 0.2046 0.1809 0.2018 0.1596 0.1146 0.1514 0.2078 0.1698 0.173 0.1347 0.1301 0.1398 0.1849 0.1418 0.212 0.1302 0.0913
Vacuole Periphery 0.7555 0.7638 0.7622 0.7765 0.797 0.8529 0.7362 0.765 0.7699 0.7413 0.7842 0.8127 0.822 0.7741 0.8053 0.7617 0.8275 0.8567

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 206.205 216.1974 228.6024 243.3094 216.0377 185.3288 242.7098 233.9801 232.2946 236.603
Translational Efficiency 0.6989 0.8235 0.7683 0.7322 0.6783 0.6954 0.7312 0.5829 0.6618 0.602

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘
Standard name
Human Ortholog
Description Subunit a of vacuolar-ATPase V0 domain; one of two isoforms (Vph1p and Stv1p); Vph1p is located in V-ATPase complexes of the vacuole while Stv1p is located in V-ATPase complexes of the Golgi and endosomes; relative distribution to the vacuolar membrane decreases upon DNA replication stress; human homolog ATP6V0A4 implicated in renal tubular acidosis, can complement yeast null mutant
Localization
Cell Percentages vacuolar membrane (57%)
Cell Cycle Regulation No
Subcompartmental Group vac memb-2

Vph1

Vph1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Vph1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available