Standard name
Human Ortholog
Description Protein involved in proteolytic activation of Rim101p; part of response to alkaline pH; PalA/AIP1/Alix family member; interaction with the ESCRT-III subunit Snf7p suggests a relationship between pH response and multivesicular body formation
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.97 0.98 0.94 0.95 0.93 0.88 0.91 0.82 0.73 0.71 0.64 0.71 0.98 0.97 0.98 0.84 0.9 0.85 0.84 0.92 0.71 0.92 0.91 0.89
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.05 0.07 0.08 0.22 0.18 0.38 0.22 0 0 0 0 0 0 0 0 0.06 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0.05 0.09 0.12 0.12 0.05 0.08 0 0 0 0.15 0.05 0.15 0 0 0.05 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 1 0 0 0 1 2 0 4 0 3 2 1 1 2 1 3 7 4 15 5 2 4 10 9
Bud 2 1 0 0 2 1 2 4 1 7 4 6 2 0 0 0 2 0 1 1 0 0 2 2
Bud Neck 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 3 4 0 0 2 2
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 3 7 0 0 2 1 2 1 1 0 1 1 0 2 0 4 4 4 0 0 0 0 0 0
Cytoplasm 320 371 34 71 193 213 294 369 142 160 129 197 308 524 739 397 251 289 300 359 63 206 453 566
Endoplasmic Reticulum 0 4 0 1 1 8 3 9 4 1 3 5 4 5 0 19 10 10 4 0 1 0 0 2
Endosome 5 1 2 0 5 9 1 3 2 4 1 4 1 6 5 21 8 12 11 4 4 4 11 17
Golgi 0 1 0 0 0 0 1 2 0 0 0 0 0 0 0 1 0 0 0 1 0 0 0 0
Mitochondria 3 1 0 1 2 11 22 38 43 41 77 62 0 1 0 3 2 2 3 2 5 1 4 4
Nucleus 0 0 0 0 1 6 4 8 4 8 6 13 0 2 0 7 5 7 0 0 0 0 0 1
Nuclear Periphery 0 0 0 0 0 0 0 1 1 2 0 0 1 0 0 1 0 0 0 0 1 0 0 0
Nucleolus 0 0 0 0 0 1 0 0 0 0 1 0 0 0 0 1 1 1 0 0 0 0 0 0
Peroxisomes 1 0 0 0 0 2 0 0 0 0 0 0 1 0 0 0 0 0 0 1 0 0 0 0
SpindlePole 1 0 1 2 4 1 1 3 0 3 3 0 1 2 2 3 1 7 6 0 2 1 8 15
Vac/Vac Membrane 2 4 0 1 5 10 16 42 23 26 11 21 3 4 10 73 15 51 8 3 4 2 2 6
Unique Cell Count 331 379 36 75 207 242 323 449 194 225 202 277 315 538 752 474 278 342 359 390 90 224 499 636
Labelled Cell Count 338 390 37 76 216 265 346 484 222 255 238 310 322 548 757 533 306 387 359 390 90 224 499 636


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.5 6.7 7.1 6.5 7.3 6.4 6.2 6.5 6.0 5.8 5.3 5.3 7.0 7.0 7.0 8.8 8.4 7.9 6.4 6.7 7.1
Std Deviation (1e-4) 0.5 0.8 0.9 1.5 1.4 1.4 1.4 1.3 5.5 1.5 5.9 1.3 0.9 0.9 0.9 1.8 1.4 1.7 0.8 0.9 1.1
Intensity Change (Log2) – – – -0.14 0.04 -0.16 -0.2 -0.13 -0.26 -0.29 -0.42 -0.42 -0.02 -0.03 -0.02 0.3 0.23 0.15 -0.16 -0.08 0.0


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 -0.3 -1.1 -0.7 -1.9 -2.8 -3.0 -3.6 -3.0 1.2 1.0 1.6 -1.7 -0.8 -1.6
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 3.1 2.8 4.5 3.2 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 2.5 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.5161 1.4266 1.1645 1.231 0.9047 1.1412 0.623 1.703 1.5022 0.7054 0.9446 1.5491 0.13 0.6507 0.5445 0.2838 0.1834 0.4897
Actin 0.0477 0.0117 0.0119 0.0018 0.0005 0.0016 0.0019 0.0003 0.0066 0.0032 0.0014 0.0008 0.0394 0.0009 0.012 0.0263 0.0001 0.0126
Bud 0.0014 0.0005 0.0008 0.0007 0 0.0001 0.0033 0.005 0.0003 0.0001 0.0072 0.0001 0.0005 0.0002 0.0008 0.0017 0 0.0004
Bud Neck 0.0077 0.0003 0.0037 0.0007 0.0004 0.0127 0.0118 0.0001 0.0004 0.0002 0.0003 0.0008 0.0008 0.0001 0.0004 0.001 0.0003 0.003
Bud Periphery 0.0021 0.0005 0.0007 0.0009 0 0.0001 0.0024 0.0009 0.0003 0 0.005 0 0.0006 0.0002 0.0009 0.0014 0 0.0007
Bud Site 0.0366 0.0042 0.0122 0.0011 0.0001 0.0001 0.0112 0.0058 0.0007 0.0001 0.0001 0.0002 0.0025 0.001 0.0066 0.004 0.0001 0.0003
Cell Periphery 0.0016 0.0003 0.0003 0.0001 0 0.0001 0.0006 0.0002 0.0001 0 0.0001 0.0002 0.0002 0.0001 0.0004 0.0001 0 0.0001
Cytoplasm 0.329 0.6754 0.6114 0.606 0.6019 0.7034 0.517 0.617 0.4925 0.5815 0.3873 0.7431 0.5406 0.7787 0.6311 0.6665 0.8716 0.726
Cytoplasmic Foci 0.0489 0.0196 0.0692 0.0477 0.1079 0.0933 0.0599 0.0155 0.0319 0.2013 0.0152 0.0433 0.0638 0.0279 0.05 0.0346 0.0125 0.0643
Eisosomes 0.0004 0.0001 0.0001 0 0 0 0.0002 0 0.0001 0 0.0002 0.0002 0.0005 0 0.0002 0.0001 0 0.0001
Endoplasmic Reticulum 0.0102 0.0049 0.0047 0.0032 0.0024 0.0073 0.0032 0.0024 0.002 0.0013 0.0043 0.0016 0.0079 0.003 0.0029 0.0028 0.002 0.003
Endosome 0.0642 0.0079 0.0326 0.0762 0.0087 0.0271 0.0074 0.0015 0.0093 0.0123 0.0035 0.0028 0.017 0.0041 0.0225 0.024 0.0048 0.0335
Golgi 0.0131 0.0029 0.0082 0.0139 0.0023 0.0024 0.0022 0.0004 0.0125 0.0012 0.0004 0.0004 0.0074 0.0006 0.0069 0.0177 0.0002 0.0041
Lipid Particles 0.0174 0.0005 0.0023 0.0071 0.0185 0.0034 0.0059 0.0001 0.0034 0.0006 0.0015 0.0018 0.0044 0.0003 0.0046 0.0217 0 0.0012
Mitochondria 0.0204 0.0077 0.002 0.0165 0.0018 0.0013 0.0028 0.0002 0.0027 0.0003 0.0017 0.0014 0.0068 0.0002 0.0084 0.0694 0.0001 0.0014
None 0.2773 0.2563 0.2238 0.2036 0.2257 0.1256 0.3335 0.347 0.4178 0.1801 0.5613 0.1836 0.2855 0.1752 0.2306 0.1048 0.1038 0.1373
Nuclear Periphery 0.0182 0.0016 0.0029 0.0019 0.0004 0.0111 0.0061 0.0005 0.0005 0.0006 0.0019 0.0005 0.002 0.0005 0.0009 0.0015 0.0006 0.0008
Nucleolus 0.0042 0.0001 0.0005 0.0003 0.0001 0.0001 0.0018 0.0001 0.0002 0 0.0003 0.0104 0.0001 0 0.0001 0.0002 0 0.0001
Nucleus 0.0234 0.0016 0.0033 0.0012 0.0008 0.0033 0.0088 0.0015 0.0012 0.001 0.003 0.0027 0.0016 0.0009 0.0014 0.0028 0.0018 0.0032
Peroxisomes 0.0322 0.0009 0.0033 0.0069 0.0266 0.0019 0.0137 0.0007 0.0022 0.0088 0.001 0.0016 0.0124 0.0009 0.0164 0.01 0.0001 0.0037
Punctate Nuclear 0.0241 0.0007 0.0033 0.0013 0.001 0.0021 0.0018 0.0004 0.0012 0.0067 0.0031 0.0036 0.0031 0.0045 0.0011 0.0009 0.0006 0.0019
Vacuole 0.0153 0.0019 0.002 0.006 0.0005 0.0019 0.0037 0.0005 0.0135 0.0003 0.0011 0.0006 0.0024 0.0006 0.0014 0.0054 0.0012 0.002
Vacuole Periphery 0.0044 0.0004 0.0007 0.0029 0.0001 0.0011 0.0009 0.0001 0.0008 0.0001 0.0003 0.0003 0.0006 0.0001 0.0004 0.003 0.0001 0.0004

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 11.9452 20.8599 16.5846 17.4702 14.0468 15.5479 20.5893 21.6919 20.1691 19.5542
Translational Efficiency 0.6299 0.2998 0.3986 0.4029 0.361 0.5134 0.4179 0.3978 0.3956 0.4482

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘
Standard name
Human Ortholog
Description Protein involved in proteolytic activation of Rim101p; part of response to alkaline pH; PalA/AIP1/Alix family member; interaction with the ESCRT-III subunit Snf7p suggests a relationship between pH response and multivesicular body formation
Localization
Cell Percentages cytoplasm (41%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Rim20

Rim20


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Rim20-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available