Standard name
Human Ortholog
Description Component of the Sum1p-Rfm1p-Hst1p complex; Rfm1p tethers the Hst1p histone deacetylase to the DNA-binding protein Sum1p; complex is involved in transcriptional repression of middle sporulation genes and in initiation of DNA replication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 – 0 0 0 0 0 0 0.05 0 0 0.06 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 – 0 0.08 0.05 0 0.07 0 0.07 0 0.05 0 0 0.09 0.16 0.05 0.09 0.07 0 0 0 0 0 0
Endoplasmic Reticulum 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.28 – 0.06 0 0 0.24 0.41 0.29 0.49 0.46 0.63 0.49 0 0 0 0.07 0.05 0.12 0 0 0 0 0 0
Nucleus 0.93 – 1.0 0.94 0.94 0.88 0.82 0.86 0.81 0.81 0.78 0.8 0.95 0.89 0.81 0.84 0.8 0.85 0.83 0.76 0.85 0.83 0.69 0.67
Nuclear Periphery 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 – 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0.05 0 0.06 0.06 0.08 0.08 0.13 0.14
Peroxisomes 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 – 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.07 0 0 0.05 0 0 0.1 0.08
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 – 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 1 1 0 0 0 0 0
Bud 0 – 0 0 2 3 5 5 3 7 6 3 0 0 0 0 0 0 1 0 0 0 1 3
Bud Neck 0 – 0 0 0 1 0 5 1 1 1 1 0 0 0 0 0 0 0 0 0 0 1 1
Bud Site 0 – 0 0 2 6 10 9 13 15 11 20 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 – 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0
Cytoplasm 2 – 0 10 18 14 28 17 19 15 14 11 16 42 88 12 13 17 0 1 0 0 3 5
Endoplasmic Reticulum 1 – 0 0 0 0 0 0 1 0 0 0 1 3 1 6 4 4 0 0 0 0 0 2
Endosome 0 – 0 0 1 0 0 0 0 0 0 1 0 2 5 4 2 3 3 0 0 1 2 7
Golgi 0 – 0 0 0 0 0 0 0 0 0 0 1 0 0 1 0 0 0 0 0 0 0 1
Mitochondria 52 – 1 5 14 80 172 116 139 158 179 169 1 2 1 16 8 31 2 1 0 0 6 8
Nucleus 174 – 18 120 323 291 349 350 228 280 222 274 345 416 436 191 117 217 161 37 40 132 247 309
Nuclear Periphery 1 – 0 1 0 2 6 0 1 3 2 1 0 1 0 0 0 2 0 0 0 0 1 1
Nucleolus 8 – 0 0 0 2 15 7 12 9 13 13 1 1 9 2 7 6 12 2 3 12 44 66
Peroxisomes 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0
SpindlePole 1 – 1 1 5 7 4 9 3 4 0 6 10 9 9 4 3 4 1 1 0 1 7 9
Vac/Vac Membrane 0 – 0 0 1 1 2 0 1 2 0 2 1 2 4 11 10 11 6 2 0 6 35 37
Unique Cell Count 188 18 128 343 332 424 406 283 347 283 343 365 466 535 227 146 256 194 49 48 159 359 461
Labelled Cell Count 239 20 137 366 407 591 519 421 494 448 501 376 478 554 247 164 297 194 49 48 159 359 461


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.4 – 4.7 5.7 5.8 4.5 4.3 4.6 4.6 4.4 3.9 4.2 6.4 6.4 6.2 6.9 7.0 6.9 6.0 6.1 6.5
Std Deviation (1e-4) 0.6 – 0.4 1.6 1.4 1.1 1.6 1.1 5.4 1.4 1.2 1.4 1.1 1.5 1.3 1.5 1.6 1.5 1.1 1.0 1.3
Intensity Change (Log2) – – – 0.28 0.29 -0.07 -0.14 -0.02 -0.04 -0.11 -0.27 -0.17 0.44 0.45 0.39 0.54 0.57 0.55 0.34 0.37 0.47


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus -1.1 -1.1 -1.6 -2.0 -1.7 -2.1 -2.1 -2.2 -2.1 -1.0 -1.5 -2.0 -1.8 -2.1 -1.8
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -0.1667 0.4558 0.0276 0.0208 -0.282 0.7618 0.2314 0.7143 0.5911 0.372 0.3539 0.7132 -0.5488 -0.2348 -0.5836 -0.5023 -0.8838 -0.6782
Actin 0.0002 0 0.0041 0 0.0021 0 0.0012 0 0.0023 0.0003 0.0006 0.0014 0.0051 0 0.0156 0 0.003 0
Bud 0.0001 0 0 0 0.0001 0 0.0005 0 0.0004 0.0001 0.0001 0.0001 0.0003 0 0.0002 0 0.0003 0.0001
Bud Neck 0.0013 0.0002 0.0001 0.0002 0.0007 0.0007 0.0014 0.0002 0.0004 0.0002 0.001 0.0011 0.0016 0.0001 0.0009 0.0001 0.0008 0.0009
Bud Periphery 0.0003 0 0 0 0.0001 0 0.0014 0 0.0004 0.0001 0.0002 0.0001 0.0009 0 0.0003 0 0.0003 0
Bud Site 0.0014 0.0001 0 0 0.0002 0 0.0027 0.0001 0.0032 0.0001 0.0003 0.0002 0.0013 0.0001 0.0011 0 0.0056 0.0001
Cell Periphery 0.0002 0.0002 0 0 0 0 0.0004 0 0 0 0 0 0.0005 0 0.0001 0 0.0001 0
Cytoplasm 0.0023 0.006 0.0065 0.0005 0.0011 0.0029 0.0015 0.0016 0.0014 0.0002 0.0007 0.001 0.0012 0.0005 0.0006 0.0001 0.0004 0.002
Cytoplasmic Foci 0.0032 0.0004 0.0009 0.0001 0.0028 0.0003 0.0072 0.0001 0.01 0.003 0.0075 0.0026 0.0043 0 0.0014 0 0.0109 0.0035
Eisosomes 0.0001 0 0 0 0 0 0.0001 0 0 0 0 0 0 0 0.0005 0 0 0
Endoplasmic Reticulum 0.0001 0.0215 0.0034 0 0.0007 0.0069 0.0007 0.0001 0.0001 0 0.0006 0.002 0.001 0 0.0001 0 0.0001 0.0001
Endosome 0.0004 0.002 0.001 0.0001 0.0049 0.0019 0.0104 0.0003 0.0088 0.0043 0.0054 0.0058 0.0067 0 0.0015 0 0.0063 0.0001
Golgi 0.0002 0 0.0004 0 0.0036 0.0008 0.0021 0 0.0043 0.0033 0.0025 0.0016 0.0035 0 0.0014 0 0.0053 0
Lipid Particles 0.0008 0.0001 0.0024 0 0.016 0 0.0055 0.0001 0.0028 0.0027 0.0391 0.0089 0.0052 0 0.0052 0 0.0134 0
Mitochondria 0.0017 0.0001 0.0002 0.0001 0.01 0.0002 0.0047 0.0001 0.0044 0.0308 0.0045 0.017 0.0025 0.0001 0.0273 0.0001 0.0022 0.0016
None 0.0159 0.0001 0.0004 0.0004 0.0008 0.0001 0.0028 0.0016 0.0005 0.0001 0.0002 0.0002 0.0003 0.0001 0.0066 0 0.0003 0.0018
Nuclear Periphery 0.0033 0.004 0.0027 0.0049 0.017 0.0015 0.0065 0.0046 0.0015 0.0016 0.0134 0.0089 0.007 0.0026 0.0029 0.0004 0.0028 0.006
Nucleolus 0.021 0.0165 0.0191 0.0195 0.0429 0.0373 0.0126 0.0152 0.0137 0.0188 0.0418 0.0282 0.0173 0.0169 0.0162 0.0153 0.054 0.0339
Nucleus 0.9312 0.9352 0.9503 0.8314 0.8443 0.9284 0.8771 0.9702 0.9371 0.8872 0.8579 0.9107 0.9289 0.9748 0.9059 0.9837 0.8623 0.9475
Peroxisomes 0.0005 0 0.0009 0 0.0065 0 0.0037 0 0.0031 0.0048 0.0194 0.002 0.0041 0 0.0021 0 0.0061 0
Punctate Nuclear 0.015 0.0129 0.0074 0.1426 0.0455 0.0181 0.0499 0.0055 0.0048 0.0419 0.0035 0.0072 0.003 0.0045 0.0095 0.0002 0.0248 0.0021
Vacuole 0.0006 0.0006 0.0001 0 0.0003 0.0003 0.0067 0.0003 0.0004 0.0003 0.0007 0.0005 0.0039 0.0001 0.0004 0 0.0006 0.0001
Vacuole Periphery 0.0002 0.0001 0.0001 0 0.0003 0.0004 0.0011 0.0001 0.0002 0.0003 0.0004 0.0006 0.0013 0 0.0003 0 0.0002 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 5.9132 5.286 8.6294 7.0125 9.9668 5.9099 6.1464 9.379 8.0969 6.4133
Translational Efficiency 2.5668 1.7324 1.168 1.557 1.0886 2.8199 1.3809 1.0986 1.3059 1.7881

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
825 366 1759 761 2292 1543 165 159 3117 1909 1924 920

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 722.18 839.91 813.87 883.31 712.09 757.63 952.84 976.37 714.76 773.41 825.79 899.39
Standard Deviation 105.02 111.35 116.73 122.70 93.63 103.75 122.71 127.02 96.88 110.12 123.55 128.38
Intensity Change Log 2 — 0.217876 0.172440 0.290561 — 0.089434 0.420174 0.455368 — 0.155535 0.300749 0.374738

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000313 0.000723 0.000835 0.001753 0.000120 0.000342 0.001809 0.002136 0.000171 0.000415 0.000918 0.001819
Bud Neck 0.002165 0.008581 0.033829 0.019942 0.003613 0.006554 0.006630 0.017536 0.003229 0.006943 0.031497 0.019526
Bud Site 0.000433 0.001063 0.003383 0.008277 0.000402 0.000974 0.001788 0.009685 0.000410 0.000991 0.003246 0.008520
Cell Periphery 0.000089 0.000056 0.000080 0.000633 0.000147 0.000093 0.000119 0.000223 0.000132 0.000086 0.000084 0.000562
Cytoplasm 0.010499 0.002986 0.002450 0.013083 0.012504 0.007693 0.007693 0.062142 0.011973 0.006790 0.002899 0.021562
Cytoplasmic Foci 0.001365 0.000963 0.000170 0.002897 0.002008 0.002255 0.000160 0.014416 0.001838 0.002007 0.000169 0.004888
Eisosomes 0.000024 0.000036 0.000032 0.000047 0.000019 0.000026 0.000061 0.000098 0.000020 0.000028 0.000034 0.000056
Endoplasmic Reticulum 0.001995 0.004138 0.004038 0.011305 0.001432 0.002555 0.009677 0.018473 0.001581 0.002858 0.004522 0.012544
Endosome 0.000653 0.000564 0.000662 0.003509 0.000279 0.001478 0.000409 0.039897 0.000378 0.001303 0.000640 0.009798
Golgi 0.000520 0.001316 0.000564 0.003474 0.000290 0.001296 0.000180 0.028750 0.000351 0.001300 0.000532 0.007842
Lipid Particles 0.000365 0.000463 0.000100 0.000502 0.000298 0.001436 0.000490 0.002294 0.000315 0.001250 0.000133 0.000812
Mitochondria 0.000758 0.004220 0.002532 0.004813 0.000710 0.005759 0.001165 0.011053 0.000723 0.005464 0.002415 0.005891
Mitotic Spindle 0.000771 0.005123 0.006628 0.020067 0.000108 0.009392 0.002624 0.115020* 0.000283 0.008574 0.006284 0.036477
None 0.015351 0.004461 0.002499 0.007446 0.012447 0.007855 0.008355 0.009308 0.013216 0.007204 0.003002 0.007768
Nuclear Periphery 0.000260 0.000319 0.001393 0.001941 0.000456 0.001450 0.000571 0.005338 0.000404 0.001233 0.001323 0.002528
Nuclear Periphery Foci 0.000869 0.000496 0.001044 0.000805 0.000176 0.000665 0.000547 0.008406 0.000359 0.000632 0.001001 0.002119
Nucleolus 0.039715 0.042890 0.036166 0.013899 0.031555 0.045104 0.021138 0.008844 0.033715 0.044679 0.034877 0.013025
Nucleus 0.922850* 0.918909* 0.899628* 0.871553* 0.932549* 0.901020* 0.932282* 0.540770* 0.929982* 0.904450* 0.902428* 0.814385*
Peroxisomes 0.000493 0.001543 0.000264 0.000534 0.000384 0.001837 0.000730 0.001389 0.000413 0.001781 0.000304 0.000682
Vacuole 0.000362 0.000808 0.002509 0.010566 0.000332 0.001507 0.003395 0.085047 0.000340 0.001373 0.002585 0.023438
Vacuole Periphery 0.000149 0.000340 0.001194 0.002952 0.000172 0.000709 0.000178 0.019176 0.000166 0.000639 0.001106 0.005756

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -4.76 -12.56 -8.85 -6.07 -5.26 -6.57 -10.74 -11.42 -10.69 -3.26 -7.61 -20.27 -12.88 -11.25 -6.93
Bud Neck -5.05 -23.60 -14.32 -8.83 4.20 -6.17 -5.26 -9.44 -8.26 -5.37 -8.16 -23.37 -16.09 -13.21 3.50
Bud Site -3.20 -5.39 -5.02 -4.59 -2.66 -3.24 -4.29 -10.36 -9.88 -7.09 -3.91 -5.95 -6.92 -6.43 -3.97
Cell Periphery 1.75 0.69 -1.52 -1.75 -1.61 2.72 0.10 -4.72 -8.03 -4.42 2.91 3.42 -1.44 -1.79 -1.82
Cytoplasm 3.28 3.84 -0.52 -4.85 -6.05 2.64 2.20 -2.98 -3.76 -3.77 3.46 7.95 -1.55 -4.27 -7.36
Cytoplasmic Foci 0.69 2.91 0.37 -0.20 -1.85 -0.55 4.08 -0.87 -0.64 -2.15 -0.51 4.89 0.60 0.99 -2.69
Eisosomes -2.77 -5.03 -9.39 -3.11 -6.96 -5.20 -7.53 -11.30 -10.51 -4.53 -5.57 -12.72 -15.21 -11.73 -9.37
Endoplasmic Reticulum -5.78 -9.64 -15.38 -11.18 -11.93 -6.20 -10.34 -10.72 -10.16 -5.06 -7.90 -15.58 -18.98 -16.89 -13.61
Endosome 0.10 0.09 -2.10 -2.58 -2.65 -3.43 -3.85 -2.99 -2.59 -2.85 -3.07 -1.36 -4.22 -2.71 -3.86
Golgi -1.38 0.04 -1.04 -0.03 -1.12 -1.91 0.14 -1.38 -1.04 -1.39 -2.18 -0.44 -1.87 -0.70 -1.70
Lipid Particles -0.73 4.76 -1.81 0.04 -7.58 -3.46 -3.11 -7.65 -2.80 -5.77 -3.51 3.90 -6.92 1.34 -9.63
Mitochondria -1.40 -6.22 -4.20 0.14 -1.34 -4.39 -4.64 -3.38 1.17 -2.59 -4.54 -6.74 -5.00 1.28 -1.74
Mitotic Spindle -1.43 -4.89 -5.98 -4.22 -4.01 -5.21 -1.77 -6.27 -5.77 -5.94 -5.33 -5.65 -8.52 -6.59 -6.70
None 4.28 5.64 3.99 -0.91 -3.70 3.00 3.37 2.27 -0.16 -0.97 4.52 9.31 5.37 0.97 -4.28
Nuclear Periphery -0.89 -3.53 -12.79 -12.41 -4.11 -3.10 -3.08 -8.25 -6.36 -7.63 -3.22 -3.19 -13.89 -7.41 -5.91
Nuclear Periphery Foci 0.48 -0.04 -0.82 -1.45 -0.83 -4.14 -3.32 -3.51 -3.32 -3.21 -2.04 -1.76 -4.42 -3.82 -2.96
Nucleolus -0.62 -1.36 9.11 7.37 12.73 -5.30 3.98 6.00 9.36 1.91 -4.83 -3.88 11.94 14.09 12.94
Nucleus 0.44 6.03 6.88 5.47 2.75 5.94 2.21 13.44 11.99 12.12 5.53 9.98 13.51 9.69 7.99
Peroxisomes -1.76 2.24 1.07 1.93 -1.89 -2.67 -2.69 -3.46 1.54 -0.81 -3.05 0.57 -0.79 2.92 -3.14
Vacuole -1.46 -3.91 -7.55 -7.14 -5.59 -3.79 -3.17 -7.60 -7.50 -6.78 -4.01 -4.89 -9.63 -9.22 -8.34
Vacuole Periphery -1.75 -1.80 -1.51 -1.24 -0.37 -2.57 -1.57 -2.01 -1.64 -1.97 -2.78 -1.79 -2.15 -1.42 -0.90
Standard name
Human Ortholog
Description Component of the Sum1p-Rfm1p-Hst1p complex; Rfm1p tethers the Hst1p histone deacetylase to the DNA-binding protein Sum1p; complex is involved in transcriptional repression of middle sporulation genes and in initiation of DNA replication
Localization
Cell Percentages nucleus (99%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-4

Rfm1

Rfm1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Rfm1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available