ORF
Human Ortholog
Description Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; expressed during copper starvation; YOR296W is not an essential gene
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0.05 0.05 0.07
Bud 0 0 0 0 0 0 0 0 0.05 – 0 0.05 0 0 0 0 0 0 0 0 0 0 0.05 0
Bud Neck 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.96 0.98 0.97 0.98 0.98 0.92 0.91 0.86 0.8 – 0.69 0.7 0.99 0.99 0.99 0.97 0.95 0.92 0.8 0.88 0.7 0.78 0.77 0.7
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0.06 0.06 0.08 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0.05 0.2 0.22 0.26 0.4 – 0.53 0.52 0 0 0 0 0 0 0 0 0.12 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 2 1 0 1 0 0 0 – 0 0 0 0 0 0 0 0 7 1 11 14 20 32
Bud 0 1 6 4 1 17 8 10 16 – 11 17 3 2 7 0 0 1 2 2 5 4 20 19
Bud Neck 1 1 7 3 5 6 8 8 2 – 1 4 0 0 0 1 1 0 5 5 8 12 11 18
Bud Site 0 0 0 1 0 0 1 1 3 – 1 4 0 0 0 0 0 0 – – – – – –
Cell Periphery 8 3 12 15 14 10 7 10 3 – 7 6 2 6 15 5 5 4 4 4 11 8 8 14
Cytoplasm 244 302 430 329 451 394 505 459 273 – 188 244 377 459 475 225 189 201 215 264 312 247 342 339
Endoplasmic Reticulum 2 0 1 0 0 0 2 0 0 – 0 2 2 1 1 15 11 17 6 2 9 3 3 7
Endosome 1 0 1 0 3 0 0 0 0 – 0 0 0 2 0 4 1 1 3 2 5 2 4 5
Golgi 2 0 0 0 0 0 0 0 0 – 0 0 0 1 0 1 1 1 0 0 1 3 1 5
Mitochondria 1 6 15 14 21 84 121 139 136 – 144 181 0 1 8 8 4 9 10 9 51 11 12 17
Nucleus 0 0 0 2 1 1 1 4 2 – 2 2 0 0 1 4 2 4 0 1 0 0 0 0
Nuclear Periphery 0 1 1 0 0 0 1 0 0 – 2 0 1 0 0 1 0 0 0 0 0 0 1 1
Nucleolus 0 0 0 1 0 0 1 2 0 – 0 0 0 0 0 0 0 1 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 – 0 1 0 0 0 0 0 0 0 0 1 0 1 0
SpindlePole 1 0 1 2 2 2 10 7 7 – 3 7 0 2 0 1 1 0 3 1 4 0 2 2
Vac/Vac Membrane 2 1 1 0 1 0 1 0 0 – 0 2 0 0 0 0 2 5 1 3 8 2 7 10
Unique Cell Count 253 307 443 337 460 427 557 532 343 272 350 379 465 478 233 198 218 269 302 443 319 444 484
Labelled Cell Count 262 315 477 372 499 515 666 640 442 359 470 385 474 507 265 217 244 269 302 443 319 444 484


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.9 4.5 3.8 3.7 3.9 3.5 3.2 3.2 2.8 – 2.9 2.7 4.5 4.6 4.1 5.8 6.3 6.1 4.6 4.8 5.5
Std Deviation (1e-4) 0.7 1.0 1.0 1.2 1.5 2.5 1.4 1.2 1.5 – 1.1 1.2 1.2 1.2 1.0 1.9 2.3 1.9 1.2 1.4 1.7
Intensity Change (Log2) – – – -0.02 0.04 -0.11 -0.21 -0.22 -0.41 – -0.37 -0.48 0.26 0.29 0.12 0.63 0.75 0.7 0.3 0.34 0.56


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.2476 3.0271 3.266 3.0507 2.6136 3.1387 2.5143 2.3623 2.1814 2.0211 2.5785 2.4335 2.4031 3.1483 2.7979 2.632 2.2408 2.8804
Actin 0.0002 0.0004 0.0033 0.0002 0.0003 0.0004 0.0574 0.0045 0.0082 0.0122 0.0133 0.0011 0.0004 0.0004 0.0024 0.0102 0.0057 0.0021
Bud 0 0.0001 0.0007 0 0 0 0.0008 0.0044 0.0031 0.0031 0.009 0.0007 0 0.0005 0.0056 0.0049 0.0108 0.0057
Bud Neck 0.0002 0.0002 0.0003 0.002 0.0001 0.0124 0.0119 0.0002 0.0025 0.0054 0.0034 0.0201 0.0001 0.0004 0.0006 0.0297 0.0008 0.0009
Bud Periphery 0.0001 0.0002 0.002 0.0001 0 0.0001 0.0015 0.0094 0.0055 0.0058 0.027 0.0013 0.0001 0.0008 0.0095 0.0063 0.0143 0.0039
Bud Site 0.0004 0.0022 0.0033 0.0005 0 0.0002 0.0085 0.0116 0.0139 0.0372 0.0166 0.0003 0.0006 0.0278 0.002 0.0555 0.001 0.0045
Cell Periphery 0.0014 0.0018 0.001 0.0006 0.0002 0.001 0.0009 0.0012 0.0006 0.0016 0.0012 0.0004 0.0015 0.0023 0.0031 0.0011 0.0009 0.0005
Cytoplasm 0.3102 0.475 0.3966 0.454 0.3405 0.6384 0.1137 0.1979 0.1805 0.1048 0.1836 0.3192 0.3876 0.2306 0.276 0.3034 0.2517 0.3472
Cytoplasmic Foci 0.0041 0.009 0.0159 0.0021 0.0017 0.003 0.0233 0.0029 0.0206 0.0073 0.0256 0.0042 0.0032 0.0047 0.0079 0.0192 0.013 0.0027
Eisosomes 0.0003 0.0002 0.0002 0.0001 0.0002 0.0001 0.0005 0.0001 0.0002 0.0004 0.0002 0.0001 0.0003 0.0003 0.0006 0.0002 0.0006 0.0001
Endoplasmic Reticulum 0.0037 0.0042 0.0035 0.0017 0.0035 0.0022 0.0051 0.0017 0.0007 0.0012 0.0017 0.0015 0.0019 0.0011 0.0023 0.0044 0.0033 0.002
Endosome 0.0012 0.0074 0.0079 0.0004 0.0003 0.0005 0.0403 0.0006 0.0072 0.0075 0.0501 0.0058 0.0004 0.0002 0.003 0.007 0.0035 0.0006
Golgi 0.0001 0.0005 0.0009 0.0001 0.0001 0.0001 0.0079 0.0005 0.0055 0.0046 0.0192 0.0032 0.0001 0.0001 0.0029 0.0021 0.0007 0.0001
Lipid Particles 0.0009 0.0006 0.0008 0.0001 0.0003 0.0001 0.0313 0.0003 0.0041 0.0051 0.0036 0.0012 0.0003 0.0003 0.0112 0.0012 0.002 0.0003
Mitochondria 0.0001 0.0002 0.0022 0.0001 0.0001 0.0001 0.0046 0.0008 0.0027 0.0355 0.0569 0.0034 0.0001 0.0001 0.0043 0.0011 0.0018 0.0002
None 0.6743 0.4936 0.5547 0.5365 0.6515 0.3403 0.622 0.762 0.7282 0.7514 0.5114 0.6291 0.6021 0.7278 0.6558 0.5438 0.6827 0.6276
Nuclear Periphery 0.0004 0.0003 0.0005 0.0002 0.0002 0.0001 0.0358 0.0002 0.0002 0.0009 0.001 0.0044 0.0002 0.0001 0.0013 0.0008 0.0011 0.0006
Nucleolus 0.0001 0.0002 0.0002 0.0001 0.0001 0.0001 0.0013 0.0001 0.0004 0.0012 0.0016 0.0001 0.0001 0.0004 0.0004 0.0005 0.0007 0
Nucleus 0.0005 0.0005 0.0006 0.0005 0.0003 0.0004 0.0111 0.0004 0.0005 0.0015 0.0023 0.0006 0.0003 0.0005 0.0014 0.0008 0.0022 0.0003
Peroxisomes 0 0.0001 0.001 0 0 0 0.0068 0.0004 0.0114 0.0051 0.0561 0.0004 0 0 0.0071 0.0029 0.0006 0.0001
Punctate Nuclear 0.001 0.0003 0.0004 0.0002 0.0002 0.0001 0.0087 0.0002 0.0028 0.0007 0.0013 0.0013 0.0002 0.0006 0.0012 0.0018 0.0007 0.0003
Vacuole 0.0007 0.0029 0.003 0.0006 0.0003 0.0004 0.0032 0.0007 0.001 0.0061 0.0117 0.0009 0.0007 0.0008 0.0011 0.0029 0.0015 0.0004
Vacuole Periphery 0 0.0002 0.0009 0 0 0 0.0036 0.0001 0.0002 0.0013 0.0033 0.0009 0 0 0.0003 0.0003 0.0003 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 4.7044 3.5683 5.4848 7.8255 6.2843 8.8337 7.86 7.3052 8.8069 10.3076
Translational Efficiency 0.6654 0.6286 0.5179 0.355 0.4263 0.2939 0.3485 0.3554 0.3638 0.3052

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
575 1600 1857 1216 1903 1677 1487 338 2478 3277 3344 1554

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 638.52 796.21 928.49 809.20 665.93 741.88 853.03 846.02 659.57 768.41 894.93 817.21
Standard Deviation 61.58 96.32 108.33 105.44 106.43 100.41 100.11 100.79 98.55 102.11 111.26 105.55
Intensity Change Log 2 — 0.318417 0.540155 0.341765 — 0.155815 0.357226 0.345321 — 0.237698 0.449667 0.343581

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000242 0.001158 0.000261 0.001526 0.000252 0.000556 0.000249 0.001227 0.000250 0.000850 0.000256 0.001461
Bud Neck 0.042577 0.047574 0.005404 0.015165 0.031805 0.072065 0.005243 0.005742 0.034305 0.060107 0.005333 0.013116
Bud Site 0.008252 0.031985 0.019116 0.040533 0.008728 0.038199 0.007897 0.032093 0.008618 0.035165 0.014127 0.038697
Cell Periphery 0.000725 0.000233 0.000283 0.000271 0.000457 0.000297 0.000272 0.000252 0.000519 0.000266 0.000278 0.000267
Cytoplasm 0.540827* 0.453010* 0.492426* 0.459228* 0.512634* 0.399507* 0.519179* 0.448496* 0.519176* 0.425630* 0.504322* 0.456894*
Cytoplasmic Foci 0.184769* 0.258283* 0.010439 0.052867 0.193315* 0.218843* 0.033047 0.038875 0.191332* 0.238100* 0.020492 0.049823
Eisosomes 0.001469 0.000470 0.000029 0.000120 0.000720 0.000239 0.000084 0.000112 0.000894 0.000352 0.000054 0.000118
Endoplasmic Reticulum 0.001366 0.000484 0.002711 0.001909 0.001628 0.000579 0.001594 0.001983 0.001567 0.000533 0.002214 0.001925
Endosome 0.010619 0.024197 0.000983 0.019132 0.011511 0.046357 0.003166 0.004998 0.011304 0.035537 0.001954 0.016058
Golgi 0.004182 0.008187 0.000058 0.011000 0.004564 0.009399 0.000268 0.000214 0.004475 0.008807 0.000151 0.008654
Lipid Particles 0.012009 0.004752 0.000683 0.004270 0.012523 0.007295 0.001676 0.002040 0.012404 0.006053 0.001125 0.003785
Mitochondria 0.004788 0.005621 0.000401 0.004425 0.004299 0.011645 0.000373 0.001251 0.004412 0.008704 0.000389 0.003734
Mitotic Spindle 0.000869 0.003093 0.004571 0.027414 0.000904 0.010109 0.003043 0.011817 0.000895 0.006683 0.003892 0.024021
None 0.002333 0.000788 0.003648 0.001804 0.010718 0.001716 0.003124 0.006704 0.008773 0.001263 0.003415 0.002870
Nuclear Periphery 0.000269 0.000119 0.001080 0.000832 0.000304 0.000206 0.000975 0.001348 0.000296 0.000164 0.001033 0.000945
Nuclear Periphery Foci 0.000247 0.000303 0.001215 0.001032 0.000494 0.000201 0.001170 0.002157 0.000437 0.000251 0.001195 0.001277
Nucleolus 0.000672 0.000520 0.000132 0.000507 0.001060 0.002018 0.000319 0.000350 0.000970 0.001286 0.000215 0.000473
Nucleus 0.115849 0.051958 0.400354* 0.137847* 0.149514* 0.049428 0.298269* 0.186497* 0.141703* 0.050663 0.354959* 0.148429*
Peroxisomes 0.005024 0.015206 0.000156 0.007299 0.004912 0.014028 0.001118 0.002243 0.004938 0.014603 0.000584 0.006199
Vacuole 0.062313 0.090960 0.055969 0.207931* 0.048887 0.114678 0.118782 0.251252* 0.052003 0.103097 0.083901 0.217354*
Vacuole Periphery 0.000600 0.001099 0.000080 0.004889 0.000770 0.002634 0.000151 0.000350 0.000731 0.001885 0.000112 0.003902

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -4.47 -2.32 -2.83 -0.12 -2.54 -4.07 -1.35 -1.93 -1.11 -1.67 -5.59 -2.38 -3.26 -1.03 -2.91
Bud Neck -0.92 7.87 6.77 13.35 -3.50 -10.95 13.40 14.07 20.90 0.86 -9.46 15.65 12.63 22.70 -3.39
Bud Site -8.18 -2.91 -5.27 2.10 -2.77 -11.46 0.78 -1.97 4.63 -2.22 -14.68 -2.67 -6.40 4.45 -4.43
Cell Periphery 3.56 3.15 3.74 1.37 1.31 1.90 3.24 4.23 2.03 3.82 3.87 4.06 5.32 2.03 2.12
Cytoplasm 7.70 10.06 13.01 6.89 4.88 12.71 7.61 12.00 4.62 7.27 13.70 12.46 17.39 6.67 7.91
Cytoplasmic Foci -8.52 26.03 21.31 38.45 -12.52 -3.85 35.67 33.78 33.66 0.67 -9.00 46.71 39.83 49.98 -8.21
Eisosomes 6.38 9.55 9.02 7.25 -14.72 6.25 8.16 7.82 8.85 -2.80 7.51 12.11 11.31 9.45 -11.25
Endoplasmic Reticulum 1.60 -4.50 -1.68 -8.81 5.88 5.11 -3.18 -4.30 -9.81 -2.29 5.14 -7.35 -3.50 -11.26 3.77
Endosome -8.69 10.70 0.55 7.90 -6.64 -14.55 9.98 8.82 18.67 -0.65 -16.38 14.10 2.45 16.22 -6.22
Golgi -3.82 5.63 -0.29 2.36 -3.63 -4.94 10.14 10.52 10.67 0.72 -6.38 11.85 0.76 4.40 -3.58
Lipid Particles 4.69 7.35 5.32 1.97 -3.24 5.14 11.97 11.40 9.16 -1.53 7.70 14.42 10.07 4.85 -2.97
Mitochondria -0.34 2.51 1.48 3.38 -3.46 -5.43 6.29 5.53 9.10 -0.74 -4.30 6.37 3.51 8.11 -3.57
Mitotic Spindle -2.09 -3.18 -5.94 -5.01 -4.06 -5.61 -2.49 -2.52 0.57 -1.45 -5.94 -4.16 -6.53 -3.56 -4.55
None 1.96 -1.26 0.63 -4.38 6.56 5.69 5.13 3.78 -2.21 -1.25 6.14 4.73 5.25 -3.42 2.73
Nuclear Periphery 1.93 -13.96 -12.19 -20.53 -0.40 2.12 -19.87 -9.38 -10.09 -2.25 3.67 -26.28 -18.18 -21.90 -1.82
Nuclear Periphery Foci -1.09 -9.20 -5.18 -4.94 1.34 3.41 -6.44 -4.54 -5.67 -1.91 2.86 -9.45 -5.48 -6.82 0.26
Nucleolus 0.74 1.54 0.99 1.49 -4.78 -1.98 6.39 6.92 3.91 1.67 -0.94 5.84 4.74 3.84 -3.28
Nucleus 12.38 -40.33 -7.42 -20.18 31.49 25.51 -23.49 -4.09 -12.72 9.24 28.70 -44.53 -5.96 -24.23 30.36
Peroxisomes -6.93 4.61 0.51 8.10 -4.78 -8.70 6.59 2.69 8.74 -0.53 -11.80 9.35 1.38 10.87 -4.30
Vacuole -6.38 -5.77 -28.87 -26.16 -25.82 -16.39 -21.58 -20.92 -16.54 -12.90 -18.37 -21.41 -37.71 -29.96 -26.23
Vacuole Periphery -4.06 5.52 -2.40 -1.64 -3.25 -9.61 8.17 6.10 13.11 -0.44 -9.79 9.92 -1.95 0.32 -3.23
ORF
Human Ortholog
Description Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; expressed during copper starvation; YOR296W is not an essential gene
Localization
Cell Percentages cytoplasm (28%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

YOR296W

YOR296W


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Yor296w-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available