Standard name
Human Ortholog
Description Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the mitochondrion; deletion confers sensitivity to 4-(N-(S-glutathionylacetyl)amino) phenylarsenoxide (GSAO); YOR305W is not an essential gene
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.06 0 0.09 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 – – 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 – – – – – –
Cell Periphery 0 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0 0
Cytoplasm 0 0.27 0.36 0.4 0.41 0.26 0.18 0.18 0.08 0.09 0.08 0.07 0.63 0.82 0.84 – – 0.33 0 0.06 0 0.08 0.09 0.05
Endoplasmic Reticulum 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0.1 0 0 0 0 0 0
Endosome 0 0 0.13 0.06 0.12 0.13 0.14 0.12 0 0 0.08 0.06 0.06 0.06 0 – – 0.22 0.16 0 0.05 0 0.05 0.06
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0 0
Mitochondria 0.94 0.88 0.09 0.42 0.12 0.51 0.53 0.52 0.89 0.81 0.87 0.81 0.18 0.06 0.05 – – 0.18 0.64 0.71 0.75 0.75 0.69 0.69
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0.06 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0.08 0.05 0 0 0 0 0 0 0.07 0.07 0 0 – – 0.08 0.09 0 0 0 0 0
SpindlePole 0 0 0.22 0.11 0.16 0.11 0.12 0.17 0.07 0.1 0.08 0.15 0 0 0 – – 0.06 0 0 0 0 0 0
Vac/Vac Membrane 0.06 0 0.11 0 0.13 0.1 0.12 0.13 0.05 0.08 0 0 0.05 0 0.05 – – 0.15 0 0.08 0 0 0.05 0.06
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 1 0 13 0 6 0 0 1 0 1 0 1 3 5 1 – – 4 0 1 8 0 5 15
Bud 0 0 5 2 2 5 5 13 17 13 11 20 5 3 2 – – 4 0 0 1 2 1 4
Bud Neck 0 0 5 0 6 1 5 4 4 4 0 3 3 0 5 – – 0 0 0 1 1 2 11
Bud Site 0 0 0 1 0 2 1 3 2 7 1 3 0 0 0 – – 1 – – – – – –
Cell Periphery 0 1 8 4 3 2 0 2 1 3 2 2 3 3 4 – – 1 0 0 0 0 2 3
Cytoplasm 0 11 51 62 74 82 63 72 37 42 27 25 115 307 316 – – 35 0 4 3 15 32 25
Endoplasmic Reticulum 0 2 2 0 4 1 2 0 1 1 2 1 2 6 4 – – 10 0 0 3 0 4 3
Endosome 0 0 18 9 22 41 51 46 7 19 25 21 11 21 15 – – 23 8 2 13 7 17 33
Golgi 0 0 0 0 2 0 0 0 0 0 0 0 1 3 0 – – 1 0 1 8 2 1 3
Mitochondria 17 36 12 65 22 161 191 206 427 380 289 286 33 23 17 – – 19 35 52 189 147 247 369
Nucleus 0 0 0 2 2 10 13 14 17 16 5 6 0 3 0 – – 6 0 0 0 0 0 2
Nuclear Periphery 0 0 0 0 0 0 0 0 3 1 0 3 0 0 0 – – 0 0 0 0 0 0 1
Nucleolus 0 0 0 1 0 0 0 2 1 3 1 2 0 0 0 – – 0 0 1 1 0 2 2
Peroxisomes 0 0 4 13 9 7 8 15 5 16 9 24 13 4 9 – – 8 4 0 8 2 4 7
SpindlePole 0 1 31 17 29 34 42 69 35 48 27 52 7 5 7 – – 6 0 1 6 4 9 14
Vac/Vac Membrane 1 0 16 7 24 32 42 52 25 39 13 12 9 5 18 – – 16 1 5 5 7 19 30
Unique Cell Count 18 41 141 156 179 318 358 399 482 467 331 354 183 375 375 105 55 74 252 198 358 538
Labelled Cell Count 19 51 165 183 205 378 423 499 582 593 412 461 205 388 398 134 55 74 252 198 358 538


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.8 3.3 5.5 5.1 5.9 4.7 4.6 5.0 3.8 4.5 4.3 4.2 4.8 4.9 4.3 – 4.9 5.2 5.4 5.5 5.6
Std Deviation (1e-4) 0.3 0.5 1.4 1.7 1.9 1.4 1.3 6.0 0.9 6.2 1.5 1.3 0.7 1.0 1.0 – 1.6 1.5 0.9 1.0 1.2
Intensity Change (Log2) – – – -0.1 0.1 -0.24 -0.26 -0.13 -0.52 -0.3 -0.36 -0.38 -0.2 -0.17 -0.35 – -0.18 -0.08 -0.03 0.01 0.03


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – 0
Bud Neck – – – – – – – – – – – – – – 0
Bud Site – – – – – – – – – – – – – – 0
Cell Periphery – – – – – – – – – – – – – – 0
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – 0
Endosome – – – – – – – – – – – – – – 0
Golgi – – – – – – – – – – – – – – 0
Mitochondria – – – – – – – – – – – – – – 0
Nuclear Periphery – – – – – – – – – – – – – – 0
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – 0
Peroxisomes – – – – – – – – – – – – – – 0
SpindlePole – – – – – – – – – – – – – – 0
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – 0
Cytoplasm – – – – – – – – – – – – – – 0
Nucleus – – – – – – – – – – – – – – 0
Vacuole – – – – – – – – – – – – – – 0.9

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -0.7897 -0.4321 -0.611 -0.9096 -0.8715 -0.6106 0.8133 1.3148 1.2808 0.7236 0.7726 1.2968 -1.3323 -0.793 -0.9241 -0.7286 -1.1821 -1.1601
Actin 0.0248 0.0364 0.0314 0.0045 0.0094 0.014 0.0465 0.0216 0.0118 0.0139 0.0278 0.0186 0.0277 0.018 0.0361 0.0344 0.0262 0.0594
Bud 0.0011 0.0022 0.0029 0.0019 0.0013 0.0014 0.0016 0.0017 0.0019 0.0036 0.0016 0.0016 0.001 0.0027 0.0038 0.0034 0.0081 0.0037
Bud Neck 0.0016 0.0011 0.0023 0.0065 0.001 0.005 0.0064 0.002 0.0059 0.0108 0.0017 0.0138 0.0025 0.0019 0.0061 0.0158 0.0011 0.0139
Bud Periphery 0.0034 0.0046 0.0074 0.0084 0.0032 0.0046 0.004 0.0052 0.0064 0.014 0.0045 0.0035 0.0017 0.0046 0.0064 0.0028 0.0255 0.006
Bud Site 0.0054 0.0297 0.0044 0.004 0.0013 0.0019 0.0226 0.0234 0.0186 0.0046 0.0018 0.004 0.0046 0.0337 0.0231 0.0206 0.0035 0.0131
Cell Periphery 0.0021 0.0016 0.0081 0.0022 0.0008 0.0017 0.0069 0.0065 0.0081 0.0019 0.0011 0.0028 0.0014 0.0026 0.0021 0.0013 0.0048 0.0016
Cytoplasm 0.0399 0.0692 0.0626 0.0766 0.0505 0.0494 0.0756 0.1292 0.0923 0.0338 0.0484 0.1188 0.0434 0.1265 0.1293 0.1305 0.1552 0.0818
Cytoplasmic Foci 0.1705 0.1494 0.1403 0.1325 0.1671 0.1439 0.1143 0.1839 0.1125 0.1491 0.0921 0.1203 0.1662 0.1572 0.2074 0.1063 0.0839 0.112
Eisosomes 0.0012 0.0023 0.0035 0.0006 0.0007 0.0013 0.0068 0.0018 0.004 0.0007 0.0008 0.0017 0.0007 0.0009 0.0012 0.0006 0.0014 0.0011
Endoplasmic Reticulum 0.0076 0.0049 0.0067 0.005 0.004 0.0043 0.0195 0.0134 0.0103 0.0016 0.0036 0.0122 0.0165 0.0151 0.007 0.0071 0.0106 0.0029
Endosome 0.1716 0.1579 0.0953 0.1834 0.2639 0.1286 0.1605 0.0629 0.0921 0.0986 0.0704 0.1124 0.2145 0.1413 0.1665 0.0854 0.1681 0.0769
Golgi 0.0736 0.0764 0.062 0.0692 0.0614 0.0569 0.0428 0.0377 0.0397 0.0298 0.0484 0.0565 0.0906 0.0639 0.0602 0.0684 0.07 0.0662
Lipid Particles 0.0479 0.0398 0.0216 0.0577 0.0152 0.0386 0.0628 0.045 0.0229 0.0304 0.0236 0.0272 0.1007 0.0216 0.0373 0.0153 0.0057 0.0548
Mitochondria 0.2614 0.2379 0.3197 0.3056 0.2449 0.3442 0.1993 0.1202 0.2563 0.3482 0.2588 0.2365 0.1473 0.1826 0.163 0.16 0.3614 0.3676
None 0.1106 0.1307 0.1602 0.0367 0.0968 0.1175 0.1648 0.2728 0.2254 0.1398 0.3069 0.1936 0.1015 0.17 0.089 0.2562 0.0243 0.0826
Nuclear Periphery 0.001 0.0016 0.001 0.0012 0.0009 0.0009 0.0028 0.0023 0.002 0.0004 0.0007 0.0017 0.0146 0.0015 0.0029 0.0168 0.001 0.0006
Nucleolus 0.0016 0.0007 0.0009 0.0007 0.0004 0.0005 0.0022 0.0011 0.0046 0.0013 0.0006 0.0006 0.001 0.0005 0.0011 0.001 0.0003 0.0003
Nucleus 0.0013 0.0008 0.0009 0.0012 0.0006 0.0007 0.003 0.0014 0.003 0.0011 0.0008 0.001 0.0038 0.001 0.0023 0.0051 0.0008 0.0006
Peroxisomes 0.0567 0.0413 0.0602 0.0899 0.0597 0.076 0.0286 0.0554 0.0533 0.0883 0.0972 0.056 0.0412 0.0391 0.0371 0.051 0.0348 0.0399
Punctate Nuclear 0.0044 0.0014 0.0018 0.0016 0.0012 0.0009 0.0016 0.0033 0.0074 0.0014 0.0006 0.0012 0.0053 0.001 0.0045 0.0119 0.0004 0.0021
Vacuole 0.0088 0.0072 0.0045 0.0063 0.0115 0.0048 0.0229 0.0076 0.0181 0.0197 0.0059 0.0116 0.0097 0.0099 0.0098 0.0037 0.008 0.0094
Vacuole Periphery 0.0034 0.0029 0.0024 0.0043 0.0041 0.0028 0.0044 0.0016 0.0034 0.0069 0.0027 0.0045 0.0042 0.0042 0.0035 0.0023 0.0049 0.0036

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 19.6766 27.0611 19.0765 20.6693 23.5492 15.1275 24.9673 24.9306 18.0744 24.8652
Translational Efficiency 0.3757 0.3233 0.2979 0.3353 0.2976 0.507 0.4305 0.389 0.3875 0.3111

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1287 782 2372 166 1843 2060 87 127 3130 2842 2459 293

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 610.65 701.46 778.79 865.27 640.94 780.34 938.43 878.83 628.49 758.64 784.44 871.15
Standard Deviation 67.54 99.28 87.15 122.56 70.75 85.57 103.49 102.78 71.03 96.23 92.60 114.61
Intensity Change Log 2 — 0.200015 0.350889 0.502805 — 0.283914 0.550060 0.455395 — 0.243589 0.456313 0.478721

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.001819 0.001967 0.007332 0.009274 0.000709 0.001347 0.012928 0.042972 0.001166 0.001517 0.007530 0.023881
Bud Neck 0.019092 0.026389 0.014628 0.023822 0.015462 0.037817 0.016784 0.021682 0.016954 0.034672 0.014704 0.022894
Bud Site 0.015110 0.037723 0.068997 0.064996 0.010494 0.014347 0.061824 0.101081* 0.012392 0.020779 0.068743 0.080637
Cell Periphery 0.000732 0.000452 0.000270 0.000209 0.000239 0.000290 0.000477 0.000317 0.000442 0.000334 0.000278 0.000256
Cytoplasm 0.030639 0.035509 0.028773 0.080104 0.032657 0.053413 0.063745 0.078240 0.031827 0.048487 0.030010 0.079296
Cytoplasmic Foci 0.131167 0.171865 0.091035 0.178192 0.148310 0.214320* 0.194181* 0.152960 0.141261 0.202638* 0.094685 0.167255
Eisosomes 0.000475 0.000204 0.000124 0.000175 0.000191 0.000248 0.000259 0.000309 0.000308 0.000236 0.000129 0.000233
Endoplasmic Reticulum 0.000925 0.000703 0.001410 0.003005 0.001388 0.000776 0.010846 0.003901 0.001198 0.000756 0.001744 0.003394
Endosome 0.035961 0.114904 0.114904 0.101069 0.046836 0.094789 0.078322 0.049844 0.042364 0.100324 0.113610 0.078865
Golgi 0.113994* 0.141859* 0.089141 0.046296 0.111207* 0.085223 0.060064 0.023224 0.112353* 0.100807 0.088113 0.036295
Lipid Particles 0.014096 0.009698 0.002129 0.008069 0.013872 0.018827 0.018791 0.005804 0.013964 0.016315 0.002719 0.007087
Mitochondria 0.549229* 0.306108* 0.354384* 0.046956 0.530780* 0.331557* 0.016909 0.032574 0.538366* 0.324555* 0.342444* 0.040722
Mitotic Spindle 0.002598 0.014505 0.064855 0.094813 0.004206 0.008976 0.038699 0.116029* 0.003545 0.010498 0.063929 0.104009*
None 0.000794 0.001230 0.000237 0.000311 0.001030 0.001310 0.000686 0.000278 0.000933 0.001288 0.000253 0.000297
Nuclear Periphery 0.000099 0.000131 0.000223 0.000657 0.000247 0.000500 0.000822 0.000376 0.000186 0.000399 0.000244 0.000535
Nuclear Periphery Foci 0.000114 0.000302 0.000342 0.001429 0.000244 0.000224 0.002887 0.001720 0.000191 0.000245 0.000432 0.001555
Nucleolus 0.000736 0.003181 0.000648 0.001306 0.001225 0.002407 0.004047 0.001091 0.001024 0.002620 0.000768 0.001213
Nucleus 0.003095 0.010059 0.003469 0.020463 0.010535 0.016332 0.052315 0.026460 0.007476 0.014606 0.005197 0.023062
Peroxisomes 0.061243 0.076270 0.071935 0.130338* 0.039650 0.063825 0.251465* 0.210944* 0.048528 0.067249 0.078287 0.165277*
Vacuole 0.007655 0.031169 0.068008 0.174281 0.016056 0.040479 0.107556 0.128118 0.012601 0.037918 0.069407 0.154271
Vacuole Periphery 0.010427 0.015771 0.017155 0.014235 0.014662 0.012993 0.006394 0.002075 0.012921 0.013758 0.016774 0.008964

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -0.31 -6.63 -3.28 -3.21 -0.56 -1.84 -5.53 -3.65 -3.58 -2.37 -1.16 -8.47 -4.37 -4.29 -2.89
Bud Neck -2.23 2.02 -0.90 0.97 -2.30 -8.75 -0.45 -1.57 4.05 -0.92 -8.59 1.44 -1.92 4.40 -2.81
Bud Site -7.15 -17.30 -5.65 -2.76 0.97 -2.78 -5.44 -6.39 -6.08 -1.98 -6.15 -19.99 -8.58 -7.39 -0.76
Cell Periphery 2.57 4.52 5.13 5.00 2.34 -0.70 -2.26 -1.20 -0.28 1.43 1.63 3.52 3.69 1.48 0.88
Cytoplasm -1.38 0.28 -4.43 -3.89 -4.53 -6.15 -2.55 -4.12 -2.58 -1.30 -6.42 0.36 -6.02 -4.13 -6.10
Cytoplasmic Foci -5.59 8.40 -2.84 0.39 -6.23 -11.18 -2.70 0.39 5.16 2.38 -13.33 12.58 -1.65 4.80 -6.79
Eisosomes 4.82 6.40 5.15 1.15 -3.12 -2.76 -2.59 -2.15 -1.02 -0.54 2.66 7.55 2.20 0.09 -4.17
Endoplasmic Reticulum 1.43 -2.29 -3.23 -3.55 -2.46 2.93 -3.46 -4.44 -5.35 2.23 3.07 -2.45 -5.11 -6.01 -3.78
Endosome -13.96 -20.76 -5.07 2.59 2.77 -13.52 -2.47 0.37 6.52 2.32 -19.64 -20.48 -3.88 4.67 6.31
Golgi -3.34 4.72 8.62 10.41 6.34 5.20 4.81 14.88 11.97 3.18 2.88 6.18 14.67 12.87 10.64
Lipid Particles 1.96 7.81 2.00 0.63 -1.99 -3.21 -0.89 5.01 7.84 1.86 -1.85 11.65 3.61 4.76 -2.62
Mitochondria 16.89 17.07 36.91* 17.76 25.81* 18.82* 55.80* 42.18* 27.16 -1.01 25.34* 22.04 54.75* 32.09 32.17*
Mitotic Spindle -5.70 -20.50* -5.15 -4.42 -1.43 -4.26 -4.03 -5.29 -5.03 -3.30 -6.96 -20.77 -7.35 -6.78 -2.53
None -0.88 3.29 2.67 1.97 -0.57 -1.05 0.67 3.52 3.55 1.04 -1.50 6.03 4.65 4.26 -0.91
Nuclear Periphery -1.20 -3.31 -3.80 -3.60 -3.04 -1.62 -2.34 -2.12 -0.44 0.89 -1.90 -1.64 -4.13 -1.93 -3.51
Nuclear Periphery Foci -3.82 -3.83 -3.41 -2.99 -2.92 0.46 -4.63 -2.37 -2.39 0.35 -1.93 -4.01 -3.70 -3.60 -3.22
Nucleolus -2.76 0.37 -1.34 2.26 -1.60 -2.69 -1.33 -0.01 2.30 1.32 -4.14 0.79 -0.63 3.45 -1.27
Nucleus -3.93 -0.86 -4.88 -3.37 -4.75 -3.15 -3.56 -2.87 -2.16 1.63 -5.40 1.60 -5.11 -3.53 -5.48
Peroxisomes -2.36 -2.23 -3.75 -2.65 -3.06 -6.89 -6.66 -6.11 -5.02 1.63 -5.94 -7.73 -7.30 -5.82 -4.96
Vacuole -8.76 -22.78 -10.65 -9.42 -7.53 -10.83 -5.15 -7.85 -6.72 -2.43 -14.49 -21.55 -13.11 -11.37 -9.03
Vacuole Periphery -3.24 -4.86 -0.34 1.01 1.39 1.44 4.25 11.98 13.24 2.37 -0.84 -3.23 2.46 2.83 3.98
Standard name
Human Ortholog
Description Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the mitochondrion; deletion confers sensitivity to 4-(N-(S-glutathionylacetyl)amino) phenylarsenoxide (GSAO); YOR305W is not an essential gene
Localization
Cell Percentages mitochondrion (89%)
Cell Cycle Regulation No
Subcompartmental Group mito-1

Rrg7

Rrg7


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Rrg7-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available