Standard name | |
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Human Ortholog | |
Description | Protein involved in ER-to-Golgi transport |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 6 | 3 | 10 | 5 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 1 | – | – | – | 4 | 5 | 11 | 4 | 15 | 11 |
Bud | 0 | 3 | 2 | 1 | 1 | 3 | 4 | 21 | 14 | 16 | 20 | 16 | 1 | 2 | 0 | – | – | – | 0 | 1 | 2 | 2 | 2 | 3 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | – | – | – | 1 | 2 | 1 | 1 | 7 | 5 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 2 | 1 | 1 | 4 | 3 | 0 | 0 | 0 | – | – | – | – | – | – | – | – | – |
Cell Periphery | 5 | 4 | 11 | 16 | 10 | 13 | 17 | 5 | 9 | 5 | 14 | 11 | 14 | 16 | 24 | – | – | – | 1 | 1 | 4 | 3 | 25 | 17 |
Cytoplasm | 37 | 68 | 68 | 68 | 141 | 188 | 314 | 224 | 229 | 265 | 233 | 288 | 107 | 222 | 209 | – | – | – | 17 | 25 | 6 | 14 | 63 | 58 |
Endoplasmic Reticulum | 94 | 110 | 236 | 70 | 70 | 74 | 15 | 18 | 4 | 5 | 3 | 2 | 87 | 73 | 121 | – | – | – | 124 | 162 | 314 | 249 | 516 | 396 |
Endosome | 0 | 0 | 4 | 7 | 8 | 8 | 9 | 4 | 7 | 3 | 4 | 5 | 0 | 0 | 1 | – | – | – | 2 | 2 | 1 | 0 | 3 | 6 |
Golgi | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | 1 | 2 | 1 | 2 | 3 | 3 |
Mitochondria | 3 | 7 | 23 | 40 | 47 | 179 | 303 | 252 | 245 | 425 | 369 | 387 | 0 | 0 | 0 | – | – | – | 4 | 4 | 6 | 17 | 42 | 26 |
Nucleus | 0 | 1 | 0 | 0 | 0 | 1 | 6 | 2 | 4 | 4 | 5 | 4 | 0 | 2 | 0 | – | – | – | 0 | 1 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 1 | 2 | 0 | 0 | 2 | 11 | 6 | 4 | 4 | 4 | 7 | 0 | 0 | 0 | – | – | – | 0 | 0 | 2 | 0 | 0 | 3 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 3 | 3 | 1 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 1 | 1 | 3 | 0 | 1 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 2 | 0 | 1 | 4 | 3 | 0 | 1 | 0 | – | – | – | 1 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 3 | 4 | 23 | 19 | 39 | 50 | 61 | 42 | 38 | 41 | 19 | 36 | 5 | 5 | 15 | – | – | – | 1 | 10 | 3 | 8 | 23 | 29 |
Unique Cell Count | 135 | 179 | 336 | 180 | 282 | 432 | 619 | 467 | 433 | 609 | 537 | 614 | 197 | 299 | 337 | 163 | 221 | 359 | 309 | 708 | 567 | |||
Labelled Cell Count | 148 | 201 | 380 | 228 | 320 | 520 | 742 | 580 | 555 | 773 | 682 | 765 | 215 | 324 | 371 | 163 | 221 | 359 | 309 | 708 | 567 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 7.0 | 6.6 | 6.8 | 6.6 | 5.5 | 4.4 | 4.0 | 4.0 | 3.8 | 3.4 | 3.5 | 3.6 | 7.1 | 7.0 | 7.8 | – | – | – | 8.1 | 8.0 | 8.2 |
Std Deviation (1e-4) | 1.0 | 0.8 | 1.0 | 1.7 | 1.0 | 1.0 | 1.0 | 0.9 | 0.9 | 0.9 | 0.7 | 0.6 | 0.7 | 0.8 | 0.8 | – | – | – | 1.3 | 1.2 | 1.2 |
Intensity Change (Log2) | – | – | – | -0.04 | -0.31 | -0.63 | -0.76 | -0.76 | -0.83 | -1.0 | -0.97 | -0.9 | 0.07 | 0.05 | 0.2 | – | – | – | 0.26 | 0.23 | 0.27 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | -0.1 | -2.5 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | -2.1 | -2.7 | – | – | – |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
Cell Periphery | 2.7 | 0.2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2.0 | 1.3 | 2.2 | – | – | – |
Cytoplasm | 4.3 | 7.8 | 6.8 | 9.2 | 8.1 | 9.2 | 7.2 | 7.0 | 8.1 | 8.1 | 13.6 | 11.0 | – | – | – |
Endoplasmic Reticulum | -6.9 | -11.2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | -5.9 | -11.5 | -8.9 | – | – | – |
Endosome | 0 | 1.5 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
Mitochondria | 5.1 | 3.8 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | -3.8 | -4.6 | -4.9 | – | – | – |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
Vacuole | 1.5 | 2.9 | 2.2 | 1.6 | 1.1 | 1.0 | -0.1 | -2.2 | -0.6 | -2.2 | -3.2 | -1.3 | – | – | – |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 3.7901 | 3.7186 | 3.7429 | 3.4851 | 3.5127 | 3.7778 | 4.6904 | 5.2605 | 5.0143 | 4.5782 | 4.9779 | 5.028 | 4.5753 | 5.1377 | 4.8253 | 5.1253 | 5.662 | 4.6113 |
Actin | 0.007 | 0.0014 | 0.0019 | 0.0052 | 0.0006 | 0.0006 | 0.0222 | 0.0002 | 0.0092 | 0.0078 | 0.0083 | 0.0118 | 0.076 | 0.0042 | 0.0207 | 0.0085 | 0.006 | 0.0064 |
Bud | 0.0001 | 0.0001 | 0.0002 | 0.0002 | 0 | 0.0045 | 0.0003 | 0 | 0.0002 | 0.0002 | 0.0001 | 0.0001 | 0.0013 | 0.0001 | 0.0004 | 0.0001 | 0 | 0 |
Bud Neck | 0.0004 | 0.0003 | 0.0004 | 0.0014 | 0.0003 | 0.007 | 0.0054 | 0.0001 | 0.0008 | 0.0009 | 0.0008 | 0.0025 | 0.0014 | 0.0008 | 0.0012 | 0.0078 | 0.0023 | 0.0081 |
Bud Periphery | 0.0001 | 0.0002 | 0.0004 | 0.0001 | 0.0001 | 0.0013 | 0.0005 | 0 | 0.0005 | 0.0003 | 0.0001 | 0.0002 | 0.0034 | 0.0002 | 0.0006 | 0.0004 | 0.0001 | 0.0001 |
Bud Site | 0.0007 | 0.0035 | 0.0037 | 0.0004 | 0.0001 | 0.0002 | 0.0031 | 0.0004 | 0.0076 | 0.0006 | 0.0002 | 0.0003 | 0.0021 | 0.0066 | 0.0027 | 0.0004 | 0.0001 | 0.0001 |
Cell Periphery | 0.0016 | 0.0034 | 0.0016 | 0.0004 | 0.0008 | 0.0007 | 0.0028 | 0.0021 | 0.0036 | 0.0014 | 0.0007 | 0.0013 | 0.0046 | 0.0123 | 0.0029 | 0.0054 | 0.0017 | 0.0015 |
Cytoplasm | 0.294 | 0.3432 | 0.293 | 0.2967 | 0.3166 | 0.345 | 0.1247 | 0.157 | 0.1643 | 0.1683 | 0.2657 | 0.2676 | 0.2026 | 0.2734 | 0.1569 | 0.2435 | 0.2803 | 0.2568 |
Cytoplasmic Foci | 0.0067 | 0.0089 | 0.0168 | 0.0063 | 0.0035 | 0.0066 | 0.0051 | 0.0047 | 0.0096 | 0.0096 | 0.0065 | 0.0133 | 0.0185 | 0.023 | 0.0078 | 0.0057 | 0.0038 | 0.0051 |
Eisosomes | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0001 | 0.0003 | 0 | 0.0003 | 0.0007 | 0.0001 | 0.0001 | 0.0009 | 0.0007 | 0.0003 | 0.0014 | 0.0001 | 0 |
Endoplasmic Reticulum | 0.6195 | 0.5268 | 0.5807 | 0.5483 | 0.6366 | 0.5481 | 0.7482 | 0.7886 | 0.7083 | 0.6655 | 0.6073 | 0.6403 | 0.6149 | 0.5613 | 0.7207 | 0.6595 | 0.6654 | 0.6972 |
Endosome | 0.0118 | 0.0269 | 0.0293 | 0.0159 | 0.0104 | 0.0112 | 0.0147 | 0.0121 | 0.0097 | 0.0418 | 0.0376 | 0.0133 | 0.0087 | 0.0124 | 0.0094 | 0.0076 | 0.0014 | 0.004 |
Golgi | 0.0018 | 0.0025 | 0.0024 | 0.0026 | 0.0006 | 0.0007 | 0.0043 | 0.0006 | 0.0043 | 0.0174 | 0.0046 | 0.0063 | 0.0074 | 0.007 | 0.0046 | 0.003 | 0.0026 | 0.0011 |
Lipid Particles | 0.0037 | 0.0093 | 0.0062 | 0.0012 | 0.0012 | 0.0014 | 0.0127 | 0.0111 | 0.0095 | 0.0161 | 0.0074 | 0.011 | 0.0121 | 0.0422 | 0.0324 | 0.0072 | 0.0013 | 0.0081 |
Mitochondria | 0.0006 | 0.0005 | 0.0009 | 0.0011 | 0.0004 | 0.0011 | 0.0021 | 0.0002 | 0.0093 | 0.005 | 0.0017 | 0.0011 | 0.002 | 0.0013 | 0.0108 | 0.0043 | 0.0001 | 0.0002 |
None | 0.029 | 0.0365 | 0.0371 | 0.1055 | 0.0184 | 0.0562 | 0.0377 | 0.0058 | 0.0399 | 0.0465 | 0.0336 | 0.0193 | 0.0247 | 0.0178 | 0.0196 | 0.0333 | 0.0313 | 0.0037 |
Nuclear Periphery | 0.01 | 0.0222 | 0.0138 | 0.0079 | 0.0054 | 0.0069 | 0.0086 | 0.0057 | 0.0041 | 0.0066 | 0.011 | 0.0034 | 0.0071 | 0.0066 | 0.0043 | 0.0054 | 0.0017 | 0.0033 |
Nucleolus | 0.0001 | 0.0001 | 0.0002 | 0.0001 | 0 | 0.0001 | 0.0001 | 0 | 0.0001 | 0.0006 | 0.0001 | 0.0001 | 0.0003 | 0.0001 | 0 | 0.0001 | 0 | 0 |
Nucleus | 0.0024 | 0.0048 | 0.0032 | 0.0018 | 0.0017 | 0.0036 | 0.0013 | 0.0013 | 0.0009 | 0.0014 | 0.0018 | 0.0011 | 0.0013 | 0.0009 | 0.0005 | 0.0013 | 0.0003 | 0.0014 |
Peroxisomes | 0.0049 | 0.0001 | 0.0005 | 0.0004 | 0 | 0.0001 | 0.0007 | 0 | 0.0103 | 0.0018 | 0.0013 | 0.0031 | 0.0048 | 0.0246 | 0.0009 | 0.0002 | 0.0001 | 0.0001 |
Punctate Nuclear | 0.0009 | 0.001 | 0.0014 | 0.0005 | 0.0004 | 0.0009 | 0.0008 | 0.0003 | 0.0007 | 0.0028 | 0.0046 | 0.0005 | 0.0027 | 0.0004 | 0.0005 | 0.0003 | 0.0001 | 0.0002 |
Vacuole | 0.0041 | 0.0075 | 0.0056 | 0.0035 | 0.0026 | 0.003 | 0.0039 | 0.0095 | 0.0061 | 0.0036 | 0.0055 | 0.003 | 0.0024 | 0.0042 | 0.0023 | 0.0039 | 0.0012 | 0.0024 |
Vacuole Periphery | 0.0004 | 0.0008 | 0.0006 | 0.0005 | 0.0002 | 0.0004 | 0.0006 | 0.0003 | 0.0009 | 0.0011 | 0.0012 | 0.0003 | 0.0008 | 0.0002 | 0.0003 | 0.0005 | 0.0001 | 0.0001 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 23.899 | 22.4506 | 15.5845 | 26.8207 | 28.3602 | 20.2422 | 28.3747 | 27.4292 | 18.4455 | 31.5709 |
Translational Efficiency | 0.667 | 0.8353 | 0.8456 | 0.3795 | 0.4458 | 0.7326 | 0.7078 | 0.5493 | 0.71 | 0.5445 |
Dataset | Hit |
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Protein Concentration | ✔ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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