Standard name
Human Ortholog
Description Alpha-arrestin involved in ubiquitin-dependent endocytosis; regulates endocytosis of plasma membrane proteins by recruiting the ubiquitin ligase Rsp5p to its targets; involved in the basal internalization and turnover of alpha-factor receptor Ste2p; recruits ubiquitin ligase Rsp5p to Ste2p via its 2 PPXY motifs; inhibited by Npr1p-mediated phosphorylation, which affects translocation between the cytosol and the plasma membrane
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.15 0.11 0.12 0 0 0 0 0 0 0 0 0 0.07 0.07 0 0.06 0.14 0.07 0.12 0.14 0.09 0.05 0 0
Bud 0 0.05 0.06 0 0 0.07 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.09 0.07 0.06 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0.07 0.05 0 0 0 0 0 0 0 0 0 0.09 0.17 0.14 0.08 0.12 0.15 0 0 0 0 0 0
Cytoplasm 0.73 0.72 0.78 0.97 0.92 0.88 0.9 0.9 0.82 0.8 0.57 0.8 0.82 0.89 0.91 0.77 0.72 0.82 0.51 0.47 0.49 0.55 0.64 0.7
Endoplasmic Reticulum 0.05 0.15 0 0 0 0 0 0 0 0 0 0 0.08 0 0 0.05 0 0 0.13 0.07 0.06 0.16 0.12 0.11
Endosome 0.09 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0.09 0.08 0.05 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.21 0.1 0.06 0.23 0.28 0.52 0.24 0 0 0 0 0 0 0 0 0.13 0 0 0
Nucleus 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.05 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 19 40 9 0 0 0 3 0 1 0 0 0 11 21 12 9 15 8 18 61 9 7 10 9
Bud 1 18 5 1 3 3 7 1 2 5 0 2 6 9 4 0 2 0 1 10 0 4 3 2
Bud Neck 0 1 1 0 1 0 0 0 0 0 0 0 0 0 0 0 1 0 8 42 7 9 8 8
Bud Site 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 5 24 4 0 0 0 2 3 4 2 0 1 14 53 44 12 13 16 1 6 1 2 4 4
Cytoplasm 93 256 61 32 119 37 128 111 117 93 12 47 127 272 285 113 79 90 78 213 53 86 162 196
Endoplasmic Reticulum 7 55 1 0 1 0 3 2 0 1 0 0 12 6 11 7 4 4 20 32 6 25 31 29
Endosome 12 4 5 1 5 0 1 1 1 0 0 2 2 9 9 9 3 2 13 36 5 3 5 8
Golgi 1 1 0 0 0 0 0 0 0 0 0 0 0 0 0 1 3 0 4 5 1 0 1 1
Mitochondria 1 4 0 0 1 9 14 7 32 33 11 14 1 1 1 3 0 0 1 13 14 4 7 4
Nucleus 0 2 1 0 2 0 3 8 1 4 0 1 0 0 1 0 1 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 1 0
Nucleolus 0 0 0 0 1 0 0 0 0 1 0 0 0 0 0 0 1 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0
SpindlePole 2 1 2 0 1 0 1 1 0 0 0 0 0 2 0 4 6 6 2 4 1 0 0 0
Vac/Vac Membrane 0 1 1 0 1 0 0 2 2 0 0 1 0 1 0 6 2 1 3 17 3 6 13 8
Unique Cell Count 128 357 78 33 129 42 142 124 142 116 21 59 154 306 314 147 109 110 155 451 110 158 254 280
Labelled Cell Count 141 407 90 34 135 49 162 136 161 139 23 68 173 376 368 164 130 127 155 451 110 158 254 280


Cytoplasm, Late Golgi

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 7.2 7.8 7.0 5.9 7.2 5.7 5.8 6.1 5.2 5.1 4.7 5.1 7.3 7.5 7.5 10.4 10.9 9.8 7.0 7.1 7.3
Std Deviation (1e-4) 0.8 0.9 1.1 0.8 1.5 1.6 1.3 1.6 1.2 1.4 0.7 0.9 0.9 1.0 1.1 1.4 1.5 1.3 1.0 1.1 1.0
Intensity Change (Log2) – – – -0.26 0.03 -0.31 -0.29 -0.22 -0.43 -0.46 -0.59 -0.46 0.04 0.08 0.08 0.56 0.63 0.48 -0.02 0.01 0.05


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 -1.1 -1.4 0 -1.4 0.4 -1.0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 1.1 2.7 2.1 0.8 1.6 2.1
Cytoplasm 2.5 2.9 1.3 2.4 2.2 0.8 0.3 -1.9 0.2 0.8 2.5 3.1 -0.2 -0.9 0.6
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 4.5 5.2 0 4.5 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 5.2864 6.3748 5.7861 5.694 5.1097 5.8992 3.9443 5.0285 4.7145 4.3621 4.2743 4.6512 3.58 3.9617 3.8067 3.6433 3.4511 3.7962
Actin 0.0632 0.0469 0.0375 0.0091 0.0438 0.0397 0.0833 0.0503 0.0916 0.0596 0.0948 0.0807 0.0469 0.0239 0.0739 0.0135 0.0798 0.0792
Bud 0.0014 0.0017 0.0022 0.0003 0.001 0.0017 0.0015 0.0059 0.0028 0.0022 0.0017 0.0018 0.0006 0.0011 0.001 0.0006 0.0011 0.0007
Bud Neck 0.0137 0.0093 0.0162 0.0049 0.0719 0.0707 0.0068 0.0058 0.0152 0.021 0.0055 0.0752 0.0034 0.0038 0.0162 0.0586 0.0074 0.0524
Bud Periphery 0.0029 0.002 0.0053 0.0004 0.0034 0.0038 0.002 0.0027 0.0066 0.004 0.0022 0.0026 0.0012 0.0008 0.0013 0.0007 0.0013 0.0019
Bud Site 0.0271 0.1427 0.0366 0.0011 0.0046 0.0037 0.0158 0.097 0.0274 0.0194 0.0026 0.0032 0.0242 0.0985 0.0519 0.0025 0.0101 0.0012
Cell Periphery 0.0254 0.0206 0.0152 0.0022 0.0399 0.0109 0.0225 0.0131 0.0176 0.0091 0.0082 0.0103 0.0555 0.0164 0.0113 0.0057 0.0098 0.0133
Cytoplasm 0.3798 0.4407 0.4895 0.691 0.5909 0.521 0.4394 0.5329 0.4758 0.4589 0.7326 0.4919 0.4644 0.5089 0.579 0.5725 0.483 0.6067
Cytoplasmic Foci 0.1503 0.1312 0.1311 0.1993 0.1028 0.1629 0.1417 0.155 0.158 0.1284 0.0738 0.1464 0.1002 0.2004 0.0903 0.131 0.0616 0.0964
Eisosomes 0.0015 0.0004 0.0007 0 0.0006 0.0003 0.0011 0.0004 0.0004 0.0002 0.0002 0.0003 0.0004 0.0001 0.0004 0 0.0001 0.0003
Endoplasmic Reticulum 0.0168 0.0082 0.0118 0.0042 0.0197 0.0138 0.0152 0.0076 0.0108 0.0143 0.0067 0.01 0.0288 0.0066 0.0178 0.0065 0.0719 0.028
Endosome 0.1061 0.0665 0.1055 0.0241 0.0543 0.0544 0.0862 0.0485 0.0675 0.1179 0.0279 0.056 0.0547 0.0431 0.0465 0.1267 0.0451 0.0454
Golgi 0.0553 0.0393 0.0445 0.0103 0.025 0.0437 0.0549 0.0383 0.0468 0.0593 0.0279 0.0588 0.0264 0.0216 0.0273 0.0553 0.0135 0.0304
Lipid Particles 0.0645 0.0415 0.0378 0.0045 0.0094 0.0243 0.0744 0.0271 0.047 0.0272 0.0082 0.0344 0.0949 0.038 0.0212 0.0136 0.02 0.022
Mitochondria 0.0193 0.0029 0.0045 0.0003 0.0018 0.0088 0.0093 0.0007 0.0026 0.0398 0.0014 0.0099 0.0013 0.0004 0.001 0.0007 0.0018 0.0012
None 0.0397 0.0196 0.0302 0.0024 0.0071 0.0087 0.0141 0.0025 0.0067 0.0046 0.0012 0.0022 0.0631 0.0045 0.0306 0.001 0.0033 0.0026
Nuclear Periphery 0.0026 0.0006 0.0019 0.0003 0.0081 0.0014 0.0064 0.0003 0.0005 0.0037 0.0002 0.0004 0.0056 0.0004 0.0145 0.0004 0.1422 0.0094
Nucleolus 0.0009 0.0004 0.0004 0 0.0003 0.0001 0.0007 0.0002 0.0002 0.0002 0 0.0001 0.0002 0.0005 0.0002 0.0001 0.0007 0.0001
Nucleus 0.0011 0.0008 0.0012 0.0004 0.0017 0.0032 0.0023 0.0005 0.0006 0.0008 0.0003 0.0004 0.0013 0.0009 0.0046 0.0003 0.0278 0.0006
Peroxisomes 0.0169 0.0119 0.0151 0.0422 0.0022 0.0202 0.008 0.0064 0.0152 0.0151 0.0026 0.0108 0.0058 0.0171 0.0033 0.0024 0.0022 0.0044
Punctate Nuclear 0.0019 0.0009 0.0022 0.0016 0.0018 0.0008 0.0071 0.0007 0.0009 0.0008 0.0003 0.0007 0.0015 0.0012 0.0017 0.0006 0.0072 0.0007
Vacuole 0.0083 0.0105 0.0096 0.0013 0.0089 0.0054 0.0064 0.0039 0.0051 0.0101 0.0015 0.0033 0.019 0.0114 0.0055 0.0066 0.0056 0.0028
Vacuole Periphery 0.0015 0.0014 0.001 0.0001 0.0009 0.0006 0.001 0.0002 0.0006 0.0036 0.0002 0.0006 0.0008 0.0003 0.0006 0.0006 0.0046 0.0005

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 20.658 17.2626 14.8949 22.4528 19.7968 15.2605 21.4118 23.4238 21.9515 22.5147
Translational Efficiency 0.5624 0.7401 0.8193 0.5365 0.5658 0.9066 0.6246 0.6476 0.5836 0.5854

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Alpha-arrestin involved in ubiquitin-dependent endocytosis; regulates endocytosis of plasma membrane proteins by recruiting the ubiquitin ligase Rsp5p to its targets; involved in the basal internalization and turnover of alpha-factor receptor Ste2p; recruits ubiquitin ligase Rsp5p to Ste2p via its 2 PPXY motifs; inhibited by Npr1p-mediated phosphorylation, which affects translocation between the cytosol and the plasma membrane
Localization
Cell Percentages cytoplasm (67%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-10

Ldb19

Ldb19


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ldb19-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available