Standard name
Human Ortholog
Description Protein involved in splicing Group I aI5-beta intron from COX1 mRNA; mitochondrial matrix protein
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0.11 0.07 0 0 0 – 0 0 0 0 0.08 0.12 0 0.11 0.1 0.06 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0.07 0 0 0 0 0 – 0 0 0 0 0.07 0 0.06 0.13 0.12 0.06 0 0 0 0 0 0
Cytoplasm 0 0 0 0.1 0.07 0.06 0 – 0 0 0.05 0 0.2 0.18 0.2 0.3 0.33 0.33 0 0 0 0 0 0
Endoplasmic Reticulum 0 0.07 0 0 0 0 0 – 0 0 0 0 0 0.07 0 0 0 0.07 0 0 0 0 0 0
Endosome 0.07 0 0.18 0.05 0.19 0.09 0.11 – 0.07 0 0 0 0.15 0.16 0.2 0.14 0.12 0.15 0.09 0.08 0.08 0.13 0.06 0.08
Golgi 0 0.21 0.13 0.13 0.08 0 0 – 0 0 0 0 0.15 0.21 0.12 0.09 0.1 0.07 0 0.1 0.05 0.06 0.06 0.05
Mitochondria 0.88 0.67 0.48 0.71 0.53 0.73 0.73 – 0.86 0.84 0.91 0.87 0.41 0.37 0.28 0.09 0.07 0.1 0.77 0.68 0.69 0.63 0.67 0.59
Nucleus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.05 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0.05 0 0 – 0 0 0 0 0.08 0.07 0.15 0 0 0 0 0 0.08 0 0 0
SpindlePole 0 0 0 0.06 0.08 0.05 0.08 – 0.06 0 0 0.05 0 0 0 0.05 0.05 0.05 0 0 0 0 0 0
Vac/Vac Membrane 0.11 0.18 0.23 0.07 0.14 0.09 0.11 – 0.12 0.1 0.07 0.08 0.16 0.08 0.09 0.31 0.25 0.34 0 0.05 0 0.08 0.08 0.11
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 8 8 8 6 1 3 – 1 4 0 1 9 20 2 35 23 14 2 4 4 0 5 7
Bud 5 2 0 4 4 7 8 – 6 2 5 11 0 5 4 3 6 5 0 2 1 0 1 1
Bud Neck 0 0 0 1 1 0 1 – 0 0 0 0 0 0 0 0 1 0 0 1 0 0 2 4
Bud Site 1 0 0 0 0 3 2 – 3 2 3 4 0 0 0 0 0 1 – – – – – –
Cell Periphery 3 18 3 2 4 3 5 – 5 2 2 5 8 7 11 41 30 15 0 0 0 0 0 0
Cytoplasm 5 8 3 11 14 14 15 – 7 7 15 7 23 30 38 93 79 79 0 1 0 1 3 7
Endoplasmic Reticulum 1 18 0 1 6 5 7 – 1 3 3 5 3 12 6 11 8 16 1 4 0 0 2 1
Endosome 11 6 13 5 39 20 44 – 20 3 2 9 17 27 38 44 30 37 14 26 11 11 15 19
Golgi 4 57 9 14 16 3 8 – 3 2 0 5 17 35 23 29 24 16 6 35 6 5 14 12
Mitochondria 142 177 34 77 106 162 282 – 231 182 248 265 48 63 54 28 16 25 129 232 97 55 163 142
Nucleus 0 3 0 1 4 7 7 – 5 5 6 5 2 0 1 6 4 7 0 0 0 1 0 1
Nuclear Periphery 2 2 0 1 1 4 12 – 5 7 3 12 0 1 1 1 0 0 0 1 1 0 2 1
Nucleolus 8 0 0 0 0 6 7 – 5 2 5 7 0 0 0 1 1 0 1 0 0 0 1 1
Peroxisomes 4 8 2 3 10 5 11 – 3 4 1 2 9 12 28 3 2 1 6 9 11 2 6 5
SpindlePole 5 3 2 7 16 10 30 – 15 3 5 15 5 3 7 15 11 11 1 0 0 0 2 2
Vac/Vac Membrane 17 49 16 8 28 21 43 – 33 22 19 23 19 14 18 96 61 83 2 15 2 7 20 25
Unique Cell Count 161 266 71 109 201 222 384 270 216 274 304 116 169 193 308 242 241 168 340 142 88 245 240
Labelled Cell Count 208 359 90 143 255 271 485 343 250 317 376 160 229 231 406 296 310 168 340 142 88 245 240


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.4 7.2 6.9 6.5 6.8 6.0 6.2 – 5.5 5.5 5.0 5.2 7.2 7.1 6.8 9.5 9.4 9.2 6.0 6.5 7.0
Std Deviation (1e-4) 0.8 1.1 1.9 1.4 1.4 1.5 1.6 – 1.4 1.2 1.6 1.0 1.4 1.4 1.4 1.8 1.9 1.6 1.0 1.0 1.3
Intensity Change (Log2) – – – -0.1 -0.03 -0.22 -0.16 – -0.34 -0.33 -0.48 -0.42 0.04 0.03 -0.04 0.45 0.44 0.41 -0.2 -0.09 0.01


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches -0.9 0 0 0 – 0 0 0 0 -0.8 0.1 0 0 -0.4 -1.6
Bud 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 – 0 0 0 0 0 0 0 2.2 2.0 0
Cytoplasm 1.4 0 0 0 – 0 0 0 0 3.0 2.8 3.1 4.5 4.8 4.8
Endoplasmic Reticulum 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Endosome -3.0 0.2 -2.2 -1.6 – -2.8 0 0 0 -0.7 -0.4 0.3 -0.9 -1.3 -0.6
Golgi 0 -1.2 0 0 – 0 0 0 0 0.4 1.5 -0.2 -0.8 -0.7 -1.6
Mitochondria 3.1 0.7 3.9 4.3 – 6.8 6.2 8.3 7.4 -0.9 -1.5 -3.0 -8.0 -8.3 -7.1
Nucleus 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 – 0 0 0 0 0 0 2.7 0 0 0
SpindlePole 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Vacuole -2.9 -1.7 -2.9 -2.6 – -2.2 -2.7 -3.9 -3.7 -1.0 -3.0 -2.8 1.4 0.5 1.9