Standard name
Human Ortholog
Description Subunit of TFIIH complex; facilities dissociation of the Ssl2p helices from TFIIH; expression levels regulated by Arg5,6p; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.81 0.72 0.64 0.57 0.37 0.29 0.34 0.28 0.24 0.21 0.21 0.62 0.59 0.56 0.2 0.22 0.2 0.66 0.51 0.54 0.55 0.58 0.53
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.16 0.13 0 0.05 0.24 0.39 0.31 0.52 0.39 0.7 0.46 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0.07 0.08 0.23 0.3 0.42 0.49 0.46 0.41 0.53 0.38 0.52 0.26 0.32 0.35 0.36 0.39 0.43 0.12 0.19 0.12 0.19 0.18 0.18
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.06
Vac/Vac Membrane 0.07 0.18 0.11 0.1 0.11 0.05 0 0 0 0.05 0.05 0.14 0.14 0.15 0.5 0.43 0.43 0.09 0.18 0.1 0.12 0.1 0.1
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 1 0 0 0 1 5
Bud 1 0 1 0 0 7 6 4 7 12 7 0 0 0 0 0 0 1 1 2 1 10 17
Bud Neck 1 0 1 5 2 5 9 1 4 2 2 0 2 2 0 0 0 1 0 0 0 4 7
Bud Site 0 0 0 0 5 5 5 8 2 6 5 0 0 0 0 0 0
Cell Periphery 1 0 0 0 1 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 184 28 116 159 100 131 163 108 107 63 80 129 145 160 64 37 48 155 26 32 51 188 228
Endoplasmic Reticulum 2 0 0 0 0 0 0 1 0 0 0 0 1 0 10 12 6 0 0 0 0 0 1
Endosome 0 0 1 2 2 1 1 0 0 0 0 6 0 1 11 3 4 3 0 1 0 4 3
Golgi 0 0 2 0 0 0 0 0 0 0 0 1 0 0 2 3 3 0 0 0 0 0 0
Mitochondria 36 5 8 13 64 176 148 199 169 212 170 5 1 0 14 3 3 10 2 2 2 5 8
Nucleus 16 3 41 83 115 221 221 155 231 115 193 55 80 98 116 65 103 29 9 7 18 57 76
Nuclear Periphery 0 0 0 0 0 0 0 1 1 0 0 0 0 0 0 0 0 0 0 1 0 0 0
Nucleolus 0 0 0 0 0 0 2 2 1 2 1 0 0 0 2 0 0 0 0 0 0 0 2
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 3 0 1 3 5 2 2 2 2 1 0 0 1 0 0 6 0 1 2 15 24
Vac/Vac Membrane 16 7 20 28 31 22 18 11 16 14 18 29 34 42 163 73 101 20 9 5 10 31 42
Unique Cell Count 228 39 180 281 272 454 479 382 438 302 373 208 247 284 325 168 237 235 52 60 94 327 433
Labelled Cell Count 257 43 193 290 321 571 578 493 540 428 479 226 263 303 383 196 268 235 52 60 94 327 433


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.3 4.8 4.8 5.3 4.2 4.0 4.5 3.9 4.2 3.8 4.2 5.3 5.3 5.4 9.1 9.2 9.5 4.4 5.2 5.5
Std Deviation (1e-4) 0.7 1.5 1.2 1.9 1.1 1.3 1.4 1.0 1.2 1.5 1.4 1.8 1.4 1.3 2.7 2.7 2.6 0.9 1.3 1.3
Intensity Change (Log2) -0.0 0.12 -0.21 -0.27 -0.1 -0.31 -0.2 -0.35 -0.19 0.13 0.14 0.15 0.92 0.93 0.98 -0.12 0.1 0.18


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -0.9 -1.8 -4.2 -5.5 -4.7 -5.5 -6.3 -6.8 -6.8 -1.2 -1.6 -1.8 -7.1 -6.0 -6.7
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 2.1 2.9 4.2 4.9 4.7 4.0 5.4 3.8 5.2 2.5 3.2 3.4 3.5 3.7 4.3
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 -0.7 -0.7 -0.5 3.8 2.9 2.9

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.7228 1.0471 1.1231 1.0092 1.1195 0.8001 -0.028 0.4581 0.3479 -0.2993 -0.1961 0.432 -0.9311 -0.7648 -0.541 -1.0514 -1.6387 -0.5053
Actin 0.0074 0.0003 0.0121 0.0006 0.0069 0.0011 0.021 0.0003 0.0157 0.0169 0.0004 0.0164 0.0528 0.0002 0.0249 0.0001 0.0003 0.0015
Bud 0.0011 0.0005 0.0009 0.0002 0.0006 0.0003 0.0005 0.0006 0.0002 0.0004 0 0.0002 0.0005 0.0001 0.0005 0.0001 0 0.0001
Bud Neck 0.0008 0.0008 0.0022 0.0002 0.002 0.0043 0.0046 0.0004 0.0014 0.0004 0.0003 0.0024 0.0009 0.0002 0.0007 0.0002 0.0006 0.0025
Bud Periphery 0.0024 0.0002 0.0013 0.0001 0.0005 0.0006 0.0007 0.0004 0.0003 0.0007 0 0.0003 0.0005 0 0.0006 0 0 0.0001
Bud Site 0.0023 0.0114 0.0144 0.0004 0.0015 0.0004 0.0032 0.001 0.0021 0.0014 0.0001 0.0003 0.0069 0.0008 0.008 0.0001 0.0001 0.0001
Cell Periphery 0.0002 0.0001 0.0002 0 0.0001 0.0001 0.0002 0.0001 0.0002 0.0001 0 0.0001 0.0002 0.0001 0.0006 0 0 0
Cytoplasm 0.1027 0.1487 0.0966 0.0458 0.0995 0.0923 0.102 0.1214 0.0951 0.0958 0.1205 0.0789 0.2283 0.1287 0.2517 0.1058 0.3368 0.2002
Cytoplasmic Foci 0.0222 0.0036 0.0139 0.0102 0.0238 0.0051 0.0176 0.002 0.0122 0.0026 0.0041 0.0044 0.0125 0.0034 0.0201 0.0014 0.0058 0.0021
Eisosomes 0.0004 0 0.0002 0.0001 0.0001 0 0.0005 0 0.0003 0.0003 0 0.0002 0.0005 0 0.0003 0.0002 0 0
Endoplasmic Reticulum 0.0036 0.0055 0.0026 0.0032 0.0012 0.0013 0.0038 0.0019 0.0022 0.0018 0.0014 0.0018 0.014 0.0027 0.0067 0.0008 0.0101 0.0016
Endosome 0.0167 0.0018 0.0075 0.077 0.0236 0.0058 0.0117 0.0014 0.0077 0.0035 0.0014 0.0053 0.0143 0.0025 0.0171 0.0006 0.0076 0.0076
Golgi 0.004 0.0001 0.0031 0.0072 0.019 0.0047 0.004 0.0001 0.0035 0.0022 0.0001 0.0028 0.0042 0.0001 0.0077 0 0.0001 0.0178
Lipid Particles 0.0109 0.0003 0.003 0.0012 0.012 0.0044 0.0054 0.0001 0.0108 0.0043 0.0001 0.0043 0.0082 0.0005 0.016 0.0001 0.0002 0.0005
Mitochondria 0.0165 0.0007 0.0208 0.01 0.0072 0.0103 0.0035 0.0007 0.0043 0.0098 0.0004 0.0014 0.0033 0.0002 0.0135 0.0004 0.0005 0.0134
None 0.2292 0.1705 0.2681 0.3174 0.1888 0.2154 0.3057 0.2382 0.2718 0.2518 0.3455 0.1856 0.2279 0.3069 0.3227 0.4865 0.5046 0.3194
Nuclear Periphery 0.0194 0.0133 0.0178 0.0071 0.0114 0.0072 0.0163 0.0085 0.0099 0.0146 0.0119 0.01 0.0196 0.0136 0.0135 0.0029 0.0246 0.0047
Nucleolus 0.0049 0.0031 0.0028 0.0018 0.0125 0.008 0.0045 0.0045 0.0028 0.0024 0.0022 0.0046 0.0019 0.0012 0.002 0.0008 0.0007 0.0018
Nucleus 0.5113 0.6187 0.4851 0.4821 0.504 0.6021 0.4564 0.5965 0.5208 0.5734 0.497 0.6404 0.3721 0.5289 0.2567 0.3975 0.0938 0.4209
Peroxisomes 0.0085 0.0001 0.0067 0.0007 0.0266 0.0016 0.0076 0.0001 0.0133 0.0027 0.0002 0.0013 0.0085 0 0.0156 0 0.0001 0.0002
Punctate Nuclear 0.0315 0.018 0.0373 0.0294 0.0572 0.0333 0.0262 0.0197 0.0237 0.0133 0.014 0.0378 0.0181 0.0056 0.0126 0.0015 0.0102 0.0044
Vacuole 0.0028 0.0019 0.0024 0.0029 0.001 0.0011 0.0034 0.0018 0.0015 0.0011 0.0004 0.0011 0.004 0.004 0.0073 0.0007 0.0033 0.0006
Vacuole Periphery 0.0012 0.0004 0.0012 0.0024 0.0006 0.0005 0.0011 0.0004 0.0004 0.0005 0.0001 0.0004 0.0008 0.0003 0.0012 0.0001 0.0006 0.0003

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 5.8805 10.5137 2.837 7.5405 9.7038 10.686 7.9728 8.8054 8.3416 10.8003
Translational Efficiency 2.0591 1.0875 3.1001 0.9843 1.1462 1.4669 1.9376 1.4161 1.816 1.7011

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1312 370 2042 930 1268 2032 909 921 2580 2402 2951 1851

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 672.91 765.86 833.41 824.08 655.82 725.25 939.62 889.51 664.51 731.51 866.13 856.64
Standard Deviation 83.37 94.98 126.22 127.97 87.91 104.45 134.34 136.50 86.06 104.09 137.80 136.27
Intensity Change Log 2 0.186667 0.308613 0.292371 0.145178 0.518778 0.439711 0.166339 0.416169 0.366974

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000274 0.000533 0.000306 0.000963 0.000093 0.000211 0.000697 0.000741 0.000185 0.000261 0.000427 0.000853
Bud Neck 0.009309 0.016459 0.015247 0.011424 0.006072 0.023011 0.004926 0.013492 0.007718 0.022002 0.012068 0.012453
Bud Site 0.002713 0.009047 0.008912 0.011612 0.002857 0.011164 0.004738 0.017153 0.002784 0.010838 0.007626 0.014369
Cell Periphery 0.000161 0.000231 0.000159 0.000204 0.000168 0.000164 0.000128 0.000185 0.000164 0.000174 0.000149 0.000194
Cytoplasm 0.377562* 0.269200* 0.109902* 0.155162* 0.395533* 0.359569* 0.067842 0.144322* 0.386394* 0.345649* 0.096946 0.149769*
Cytoplasmic Foci 0.178101 0.149235 0.001313 0.008129 0.104984 0.092738 0.000927 0.004752 0.142166 0.101440 0.001194 0.006449
Eisosomes 0.000122 0.000117 0.000019 0.000047 0.000069 0.000068 0.000031 0.000036 0.000096 0.000076 0.000023 0.000041
Endoplasmic Reticulum 0.000922 0.003131 0.003952 0.007876 0.001278 0.000899 0.008131 0.005581 0.001097 0.001243 0.005239 0.006734
Endosome 0.006740 0.009525 0.000600 0.003002 0.004071 0.007965 0.000743 0.003223 0.005428 0.008205 0.000644 0.003112
Golgi 0.001949 0.001513 0.000045 0.004563 0.000474 0.002660 0.000066 0.001071 0.001224 0.002483 0.000052 0.002825
Lipid Particles 0.010047 0.010607 0.000361 0.001369 0.005526 0.004153 0.000606 0.000660 0.007825 0.005147 0.000437 0.001017
Mitochondria 0.007118 0.007324 0.001789 0.004483 0.003932 0.007461 0.001046 0.002767 0.005552 0.007440 0.001560 0.003630
Mitotic Spindle 0.001513 0.003416 0.004940 0.019109 0.001044 0.010686 0.004028 0.030424 0.001283 0.009566 0.004659 0.024739
None 0.005850 0.004091 0.004725 0.008124 0.009325 0.005627 0.004681 0.008089 0.007558 0.005390 0.004711 0.008107
Nuclear Periphery 0.000907 0.001013 0.001781 0.003280 0.000947 0.000919 0.001498 0.001963 0.000927 0.000934 0.001694 0.002625
Nuclear Periphery Foci 0.000542 0.001420 0.000320 0.002476 0.000708 0.000434 0.000498 0.000475 0.000624 0.000586 0.000374 0.001480
Nucleolus 0.002061 0.003157 0.000472 0.002346 0.002121 0.003503 0.000610 0.001966 0.002091 0.003450 0.000514 0.002157
Nucleus 0.362080* 0.457466* 0.832377* 0.713622* 0.438422* 0.435016* 0.881946* 0.726411* 0.399600* 0.438474* 0.847646* 0.719985*
Peroxisomes 0.008939 0.012794 0.000088 0.001301 0.002145 0.006551 0.000727 0.000759 0.005600 0.007513 0.000285 0.001031
Vacuole 0.022451 0.038423 0.012504 0.039593 0.019507 0.025728 0.016024 0.035530 0.021004 0.027683 0.013588 0.037571
Vacuole Periphery 0.000639 0.001298 0.000189 0.001315 0.000724 0.001473 0.000107 0.000400 0.000681 0.001446 0.000164 0.000860

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -1.98 -0.72 -3.45 -2.00 -4.20 -4.55 -11.84 -8.35 -6.82 1.61 -1.22 -5.41 -6.84 -7.55 -3.58
Bud Neck -2.57 -4.10 -1.82 1.44 2.02 -9.81 -1.64 -6.00 4.05 -4.34 -9.38 -5.13 -5.31 5.06 0.06
Bud Site -3.86 -4.39 -3.36 0.29 -0.34 -5.63 -1.62 -3.97 0.51 -2.43 -6.86 -4.52 -5.19 0.92 -1.56
Cell Periphery -2.74 0.51 -0.24 2.57 -0.70 0.38 1.13 0.02 -0.38 -1.17 -0.75 1.07 -0.15 0.62 -1.21
Cytoplasm 7.35 33.74 25.32 9.48 -3.77 3.58 38.96 28.56 28.55 -8.62 5.22 49.56 38.07 31.14 -6.84
Cytoplasmic Foci 2.57 31.02 30.01 14.24 -4.68 2.28 23.60 22.99 26.66 -5.51 8.30 38.22 37.12 29.64 -6.21
Eisosomes 0.19 14.02 8.54 6.99 -12.19 0.22 5.34 3.13 3.00 -2.76 4.00 16.16 8.96 5.60 -12.91
Endoplasmic Reticulum -5.35 -17.89 -16.38 -8.80 -8.16 2.07 -16.37 -14.52 -16.15 3.58 -1.56 -22.81 -21.89 -21.47 -5.07
Endosome -2.14 11.36 7.61 5.25 -4.88 -4.52 6.43 3.40 7.18 -2.21 -3.84 13.06 7.80 8.92 -5.01
Golgi 1.04 5.86 0.12 -0.27 -1.56 -4.31 5.34 0.32 4.18 -1.66 -2.70 6.88 0.19 1.88 -1.83
Lipid Particles -0.19 12.19 10.82 4.75 -4.78 1.96 7.53 7.33 9.43 -1.32 4.16 14.38 13.02 9.50 -5.67
Mitochondria -0.01 3.91 2.91 1.70 -0.95 -2.62 2.44 1.50 5.69 -2.48 -1.64 4.38 3.24 5.38 -1.53
Mitotic Spindle -0.95 -3.09 -5.56 -4.60 -4.28 -5.49 -2.78 -6.47 -3.65 -4.89 -5.51 -4.26 -8.52 -4.77 -6.54
None 1.76 1.64 -0.43 -2.55 -2.78 2.63 3.81 1.75 -1.47 -3.77 2.55 3.99 1.14 -2.04 -4.50
Nuclear Periphery -0.71 -9.79 -6.84 -6.33 -3.82 0.03 -8.66 -12.88 -13.32 -7.06 -0.35 -13.33 -11.77 -11.66 -6.53
Nuclear Periphery Foci -3.78 0.59 -2.98 -1.00 -3.11 1.61 -1.33 -1.45 -3.40 -0.17 0.12 0.12 -3.31 -3.36 -3.32
Nucleolus -1.85 8.04 0.55 1.81 -2.23 -2.23 3.14 0.77 3.17 -2.75 -2.91 6.71 0.92 3.29 -3.48
Nucleus -4.78 -51.35 -27.94 -12.88 11.23 0.30 -40.70 -21.70 -23.67 13.36 -4.35 -63.70 -35.02 -29.12 16.69
Peroxisomes -1.66 8.88 7.84 5.90 -1.71 -7.32 3.45 6.60 11.38 0.99 -2.44 9.95 9.20 12.08 -1.07
Vacuole -4.51 2.98 -10.78 -5.10 -11.77 -3.35 -4.01 -11.70 -10.51 -8.67 -4.66 -0.60 -15.91 -13.36 -15.32
Vacuole Periphery -2.02 7.18 0.11 1.65 -1.64 -2.05 3.60 2.66 3.66 -2.86 -2.60 5.86 1.53 3.24 -2.05
Standard name
Human Ortholog
Description Subunit of TFIIH complex; facilities dissociation of the Ssl2p helices from TFIIH; expression levels regulated by Arg5,6p; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus
Localization
Cell Percentages cytoplasm (21%), nucleus (12%), mixed (42%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Tfb6

Tfb6


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Tfb6-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available