Standard name
Human Ortholog
Description Sorting nexin for late-Golgi enzymes; required to maintain late-Golgi resident enzymes in their proper location by recycling molecules from the prevacuolar compartment; contains a PX domain and sequence similarity to human Snx3p
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 – 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0.13 – 0 0 – 0.05 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 – 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 – 0 0 – 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 – 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0.1 0.1 – 0.18 0.15 – 0.17 0.12 0.17 0.14 0.18 0.07 0.08 0.06 0.16 0.27 0.18 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 – 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0.12 0.52 0.41 – 0.62 0.3 – 0.39 0.18 0.22 0.17 0.26 0.79 0.81 0.81 0.53 0.51 0.52 0.77 0.79 0.71 0.77 0.79 0.83
Golgi 0 0 0 – 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.38 0 0.44 – 0.16 0.48 – 0.45 0.66 0.6 0.64 0.56 0 0 0 0 0.07 0 0 0 0.05 0 0 0
Nucleus 0 0 0 – 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 – 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.19 0.06 0.08 – 0.05 0.07 – 0 0.07 0.08 0.05 0.07 0.11 0.1 0.09 0.11 0.06 0.07 0 0 0 0 0 0
Peroxisomes 0 0 0 – 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0.12 0.05 0 – 0.07 0 – 0.06 0.08 0.09 0 0.08 0.13 0.13 0.15 0.14 0.12 0.16 0.05 0 0 0.05 0.05 0
Vac/Vac Membrane 0.5 0.65 0.31 – 0.25 0.22 – 0.12 0.1 0.08 0.13 0.06 0.43 0.28 0.29 0.54 0.47 0.46 0.1 0.13 0.1 0.08 0.06 0.07
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 – 0 0 – 1 0 2 0 0 4 1 0 3 2 2 0 0 1 0 1 3
Bud 0 0 5 – 0 2 – 9 7 7 8 8 1 0 3 0 0 0 0 1 0 2 2 0
Bud Neck 0 0 0 – 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 – 0 0 – 0 0 0 0 1 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 1 0 – 0 0 – 0 0 0 1 1 0 0 0 1 0 0 0 0 0 0 0 0
Cytoplasm 0 23 4 – 10 14 – 30 15 27 27 36 21 26 22 24 24 21 0 1 1 1 1 2
Endoplasmic Reticulum 0 2 0 – 0 2 – 0 1 4 5 7 5 5 3 3 2 2 0 0 0 4 1 1
Endosome 2 115 16 – 34 28 – 69 22 35 33 51 236 279 276 78 46 62 27 184 52 182 202 281
Golgi 0 0 0 – 0 0 – 0 0 0 1 0 0 1 0 3 1 2 0 2 0 2 3 4
Mitochondria 6 5 17 – 9 45 – 79 81 94 126 110 1 5 1 6 6 1 0 3 3 4 6 5
Nucleus 0 3 0 – 0 0 – 0 1 0 0 0 0 0 1 1 0 3 0 1 0 0 0 1
Nuclear Periphery 0 2 0 – 0 1 – 0 2 0 1 1 2 8 2 1 0 0 0 1 0 0 1 0
Nucleolus 3 13 3 – 3 7 – 5 9 12 10 13 34 36 31 16 5 8 0 1 1 0 0 1
Peroxisomes 0 0 0 – 2 0 – 3 2 1 1 4 0 1 2 1 1 1 0 0 0 0 0 1
SpindlePole 2 11 0 – 4 1 – 10 10 14 8 15 38 45 52 20 11 19 1 3 3 12 13 9
Vac/Vac Membrane 8 142 12 – 14 21 – 21 12 13 25 12 128 97 98 79 42 55 3 29 7 18 15 24
Unique Cell Count 16 220 39 55 94 177 123 157 198 196 298 343 339 147 90 119 36 233 74 236 256 340
Labelled Cell Count 21 317 57 76 121 227 162 209 246 259 470 504 491 236 140 176 36 233 74 236 256 340


Vacuole Periphery

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.8 8.0 5.6 – 6.6 5.5 – 5.6 4.9 5.0 5.4 5.5 7.2 7.6 7.6 11.8 11.0 11.7 7.2 7.7 7.9
Std Deviation (1e-4) 0.7 1.1 1.3 – 2.0 1.4 – 1.4 1.1 1.2 1.5 1.5 1.3 1.2 1.1 2.5 2.5 2.4 1.4 1.6 1.9
Intensity Change (Log2) – – – – 0.23 -0.04 – -0.01 -0.18 -0.17 -0.07 -0.03 0.37 0.44 0.43 1.07 0.97 1.06 0.35 0.46 0.49


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches – 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud – 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Neck – 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Site – 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery – 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm – 1.1 0.7 – 1.0 0 1.1 0.6 1.2 0 0 0 0.9 2.1 1.1
Endoplasmic Reticulum – 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Endosome – 2.0 -1.3 – -0.2 -3.0 -2.4 -3.4 -1.9 5.2 5.7 5.7 1.3 1.1 1.2
Golgi – 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Mitochondria – -2.9 0.5 – 0.1 2.5 1.8 2.3 1.4 0 0 0 0 -5.0 0
Nucleus – 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery – 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Nucleolus – 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes – 0 0 – 0 0 0 0 0 0 0 0 0 0 0
SpindlePole – 0 0 – 0 0 0 0 0 0 0 2.6 0 0 0
Vacuole – -0.6 -1.0 – -3.0 -3.2 0 -2.9 0 1.5 -0.3 -0.2 2.6 1.7 1.7

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.2035 2.0429 1.6324 1.5812 0.9779 1.9673 0.0733 1.088 0.9091 0.7921 0.6391 1.0321 2.6385 3.1228 2.9437 3.3012 3.0539 2.92
Actin 0.0037 0 0.0191 0 0.0908 0 0.0034 0 0.0197 0.0004 0.0001 0.0001 0.014 0.0001 0 0 0 0
Bud 0.0002 0.0001 0.0003 0.0001 0.0066 0 0.0002 0.0001 0.0017 0.0013 0.0001 0.0001 0.0002 0.0002 0.0001 0 0 0.0001
Bud Neck 0.0009 0.0031 0.0016 0.0001 0.0021 0.0003 0.0005 0.0006 0.0013 0.0017 0.0004 0.0019 0.0008 0.0013 0.0009 0.0002 0.0007 0.0015
Bud Periphery 0.0002 0.0001 0.0003 0 0.0087 0 0.0002 0.0001 0.0012 0.0017 0 0 0.0002 0.0002 0.0001 0 0 0.0001
Bud Site 0.0014 0.0007 0.0031 0.0001 0.0093 0.0002 0.0018 0.0011 0.017 0.0037 0.0002 0.0006 0.0009 0.0036 0.0007 0.0001 0.0004 0.001
Cell Periphery 0.0002 0.0001 0.0002 0 0.0004 0 0.0002 0.0002 0.0002 0.0006 0 0.0001 0.0001 0.0002 0.0001 0 0 0.0001
Cytoplasm 0.037 0.0498 0.026 0.0048 0.0037 0.0086 0.0206 0.0373 0.0128 0.0054 0.0018 0.021 0.0201 0.0478 0.0202 0.0081 0.003 0.0264
Cytoplasmic Foci 0.1302 0.1735 0.129 0.1249 0.1252 0.1454 0.1347 0.1748 0.1772 0.111 0.1831 0.179 0.1509 0.1197 0.1435 0.1484 0.1908 0.1582
Eisosomes 0.0002 0 0.0001 0 0.0004 0 0.0001 0 0.0001 0 0 0 0.0003 0 0 0 0 0
Endoplasmic Reticulum 0.003 0.0022 0.0022 0.0003 0.0033 0.001 0.0012 0.0013 0.0017 0.0007 0.0003 0.0006 0.0049 0.0028 0.0041 0.0005 0.0002 0.0016
Endosome 0.5959 0.5586 0.6526 0.8026 0.4402 0.7173 0.5991 0.6056 0.6064 0.6219 0.7143 0.6188 0.6141 0.6088 0.6391 0.7441 0.674 0.6114
Golgi 0.0204 0.0229 0.0361 0.0185 0.0771 0.0298 0.033 0.0193 0.0382 0.0352 0.0274 0.031 0.021 0.0178 0.0315 0.0259 0.0315 0.0328
Lipid Particles 0.0799 0.0872 0.0321 0.0081 0.0858 0.0527 0.0974 0.0804 0.0285 0.0587 0.0268 0.05 0.0709 0.067 0.0577 0.0466 0.077 0.0536
Mitochondria 0.0093 0.001 0.0035 0.0007 0.0394 0.0005 0.0105 0.0012 0.0226 0.0163 0.0008 0.0259 0.0025 0.0072 0.0011 0.0007 0.0003 0.0066
None 0.0233 0.0235 0.0169 0.0004 0.0017 0.0012 0.0146 0.0166 0.0043 0.0003 0.0001 0.0007 0.0316 0.0108 0.0288 0.0016 0.0001 0.0021
Nuclear Periphery 0.0037 0.0025 0.0021 0.0004 0.0149 0.001 0.0011 0.0011 0.0012 0.001 0.0004 0.0005 0.0075 0.0057 0.0049 0.0005 0.0003 0.0019
Nucleolus 0.005 0.0006 0.0111 0.0002 0.0008 0.0035 0.0066 0.0029 0.0007 0.0173 0.0001 0.0016 0.0014 0.0093 0.0064 0.0003 0.0005 0.0042
Nucleus 0.0027 0.001 0.0044 0.0002 0.0009 0.0004 0.0014 0.0013 0.0006 0.0035 0.0001 0.0011 0.0025 0.0062 0.0012 0.0001 0.0002 0.0101
Peroxisomes 0.0187 0.0063 0.0059 0.0024 0.0444 0.0032 0.0159 0.0059 0.0219 0.016 0.0166 0.0108 0.0067 0.0076 0.0108 0.0084 0.0053 0.01
Punctate Nuclear 0.0055 0.0035 0.0021 0.0003 0.0026 0.0024 0.0012 0.0014 0.0014 0.0011 0.0005 0.0201 0.0034 0.0029 0.0025 0.0005 0.0013 0.0081
Vacuole 0.0528 0.0558 0.0436 0.029 0.0227 0.0273 0.048 0.0442 0.0343 0.086 0.0203 0.0315 0.0404 0.0734 0.0377 0.0103 0.0122 0.06
Vacuole Periphery 0.0058 0.0076 0.0077 0.007 0.0189 0.005 0.0082 0.0047 0.0071 0.0161 0.0066 0.0045 0.0058 0.0075 0.0087 0.0034 0.0023 0.0099

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 25.9492 14.1199 26.9423 39.3281 35.107 18.0416 32.1224 44.0492 39.5198 43.3071
Translational Efficiency 1.6695 2.5385 1.2792 1.1277 1.2768 3.3505 1.692 1.4923 1.6668 1.4007

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
2074 1687 220 1491 1506 1348 231 1508 3580 3035 451 2999

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 714.64 856.86 1085.71 1009.65 618.23 860.76 1043.42 1001.10 674.08 858.59 1064.05 1005.35
Standard Deviation 104.88 117.02 138.69 136.50 99.01 110.54 110.61 133.32 112.97 114.21 126.87 134.98
Intensity Change Log 2 — 0.261843 0.603350 0.498567 — 0.477467 0.755104 0.695371 — 0.365875 0.675728 0.593198

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000090 0.000076 0.000691 0.000598 0.000113 0.000071 0.000585 0.000727 0.000099 0.000074 0.000637 0.000662
Bud Neck 0.008813 0.004180 0.017459 0.006186 0.016655 0.003781 0.009632 0.004684 0.012112 0.004003 0.013450 0.005430
Bud Site 0.006558 0.004422 0.018470 0.025266 0.009431 0.004599 0.015820 0.025122 0.007767 0.004501 0.017113 0.025194
Cell Periphery 0.000062 0.000029 0.000090 0.000081 0.000160 0.000058 0.000052 0.000046 0.000103 0.000042 0.000071 0.000063
Cytoplasm 0.023408 0.006437 0.003727 0.021397 0.053720 0.002810 0.013262 0.032771 0.036159 0.004826 0.008611 0.027116
Cytoplasmic Foci 0.174032 0.112947 0.154824 0.140492 0.151229 0.100833 0.094073 0.166430 0.164439 0.107566 0.123708 0.153534
Eisosomes 0.000046 0.000013 0.000041 0.000016 0.000072 0.000017 0.000035 0.000014 0.000057 0.000015 0.000038 0.000015
Endoplasmic Reticulum 0.000485 0.000236 0.001452 0.000668 0.001482 0.000309 0.001847 0.000299 0.000904 0.000268 0.001654 0.000482
Endosome 0.607897* 0.688525* 0.358508* 0.369470* 0.536004* 0.712531* 0.433462* 0.406151* 0.577654* 0.699187* 0.396899* 0.387914*
Golgi 0.061278 0.038303 0.019678 0.041557 0.034628 0.044664 0.009705 0.065405 0.050067 0.041128 0.014570 0.053548
Lipid Particles 0.018494 0.008200 0.019971 0.002759 0.030195 0.011506 0.005935 0.002380 0.023416 0.009668 0.012782 0.002568
Mitochondria 0.013928 0.006011 0.002271 0.006357 0.015924 0.017165 0.001005 0.008082 0.014768 0.010965 0.001623 0.007224
Mitotic Spindle 0.008269 0.023218 0.125657* 0.102202* 0.002426 0.010794 0.047918 0.073027 0.005811 0.017699 0.085839 0.087532
None 0.001254 0.000334 0.000055 0.000115 0.007108 0.000086 0.000174 0.000053 0.003716 0.000224 0.000116 0.000084
Nuclear Periphery 0.000317 0.000350 0.004100 0.001238 0.000974 0.000249 0.002071 0.000460 0.000593 0.000305 0.003061 0.000847
Nuclear Periphery Foci 0.002334 0.004691 0.063083 0.006210 0.005158 0.003664 0.015695 0.003221 0.003522 0.004235 0.038811 0.004707
Nucleolus 0.002479 0.001572 0.006661 0.001614 0.010411 0.001155 0.007537 0.000785 0.005816 0.001387 0.007109 0.001197
Nucleus 0.004020 0.000513 0.007894 0.001707 0.019154 0.000552 0.009457 0.000723 0.010386 0.000531 0.008695 0.001212
Peroxisomes 0.010284 0.009335 0.044619 0.011009 0.014318 0.010443 0.020095 0.012626 0.011981 0.009827 0.032058 0.011822
Vacuole 0.046676 0.069046 0.130951 0.235431* 0.077035 0.056216 0.293073* 0.173299 0.059447 0.063348 0.213989* 0.204189*
Vacuole Periphery 0.009277 0.021562 0.019798 0.025631 0.013806 0.018496 0.018567 0.023695 0.011182 0.020200 0.019168 0.024657

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 0.47 -3.56 -3.19 -3.24 0.61 1.96 -1.85 -2.41 -2.59 0.59 1.38 -2.96 -3.77 -3.94 0.78
Bud Neck 4.26 -3.36 1.82 -3.33 4.10 8.15 2.94 7.78 -1.76 3.92 8.84 -1.41 7.17 -3.58 5.43
Bud Site 2.63 -4.92 -9.26 -10.52 -1.71 4.02 -2.98 -8.66 -11.47 -1.83 4.69 -5.54 -12.91 -15.59 -2.51
Cell Periphery 4.28 -3.11 -0.64 -2.17 0.70 4.23 4.75 5.73 0.83 1.19 5.46 2.57 2.85 -1.56 1.07
Cytoplasm 7.77 8.45 -1.73 -9.68 -10.32 15.20 6.13 3.84 -13.43 -3.50 16.49 7.97 1.35 -16.19 -7.12
Cytoplasmic Foci 12.85 2.55 8.01 -4.49 1.36 10.34 8.22 -1.83 -12.13 -9.44 16.58 7.20 4.60 -11.66 -4.58
Eisosomes 3.31 0.16 2.96 -2.02 7.10 11.85 4.85 12.88 2.06 5.95 6.86 2.08 6.83 -0.15 9.10
Endoplasmic Reticulum 2.33 -3.68 -1.34 -4.31 3.14 4.25 -1.81 4.23 -0.29 5.63 4.82 -4.10 2.94 -3.97 6.25
Endosome -9.08 14.87 28.92* 37.30* -0.35 -16.99 6.66 13.99 33.65 0.53 -18.00 15.22 30.71 49.87* 0.19
Golgi 8.26 14.84 7.57 0.21 -6.78 -3.60 11.80 -7.82 -4.48 -15.86 4.38 19.32 0.09 -3.72 -16.41
Lipid Particles 5.53 -0.25 10.59 4.43 4.87 7.41 10.03 12.85 6.35 4.02 9.07 4.93 16.59 7.60 5.78
Mitochondria 6.49 8.78 5.41 0.07 -2.80 -0.55 11.93 4.69 3.95 -5.25 3.02 14.54 6.96 3.26 -5.71
Mitotic Spindle -6.04 -7.83 -17.56 -14.00 1.23 -5.69 -5.05 -15.95 -13.60 -2.14 -7.74 -9.09 -23.45 -19.06 -0.27
None 2.52 4.10 3.90 0.83 -2.10 5.67 5.59 5.70 1.15 1.64 6.21 6.57 6.64 0.95 0.77
Nuclear Periphery -0.47 -4.31 -6.26 -6.44 3.05 4.72 -2.76 2.78 -4.48 3.76 3.60 -5.23 -3.34 -7.52 4.48
Nuclear Periphery Foci -2.65 -6.95 -5.09 -1.76 6.40 1.24 -3.00 1.47 0.10 3.37 -1.10 -7.44 -2.46 -1.06 7.08
Nucleolus 1.16 -2.11 1.18 -0.29 2.33 6.40 1.33 7.19 0.79 4.18 5.92 -1.41 6.93 0.41 4.02
Nucleus 4.55 -1.50 2.03 -5.31 1.89 9.43 2.23 9.17 -1.50 4.00 10.37 -0.34 9.01 -4.97 3.72
Peroxisomes 0.95 -5.60 -0.22 -1.07 5.54 2.51 -1.73 1.23 -1.29 2.46 2.44 -5.82 0.58 -1.77 5.97
Vacuole -6.03 -7.64 -29.94 -25.33 -8.18 4.70 -13.19 -17.01 -20.50 6.85 -1.32 -14.55 -34.00 -32.12 0.69
Vacuole Periphery -8.11 -3.00 -10.47 -1.15 -1.40 -3.10 -1.31 -5.79 -2.31 -2.07 -8.36 -3.36 -11.76 -2.28 -2.41
Standard name
Human Ortholog
Description Sorting nexin for late-Golgi enzymes; required to maintain late-Golgi resident enzymes in their proper location by recycling molecules from the prevacuolar compartment; contains a PX domain and sequence similarity to human Snx3p
Localization
Cell Percentages endosome (52%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Snx3

Snx3


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Snx3-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available