Standard name
Human Ortholog
Description High-affinity cyclic AMP phosphodiesterase; component of the cAMP-dependent protein kinase signaling system, protects the cell from extracellular cAMP, contains readthrough motif surrounding termination codon
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.09 0 0.08 0.09 0.1
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.19 0.08 0.15 0.2 0.12 0.09 0.08 0.08 0.07 0 0.07 0 0.31 0.31 0.43 0.34 0.34 0.29 0.08 0 0.07 0.15 0.1 0.13
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0.05 0 0 0 0 0 0
Endosome 0 0 0.1 0.07 0.06 0 0 0 0 0 0 0 0.09 0.12 0.08 0.09 0.09 0.06 0.05 0 0.07 0 0.05 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.05 0 0 0 0.06 0.21 0.3 0.18 0.33 0.4 0.45 0.47 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0.25 0.19 0.22 0.36 0.43 0.49 0.45 0.58 0.57 0.5 0.62 0.54 0.1 0.05 0.05 0.25 0.28 0.24 0.58 0.57 0.45 0.35 0.41 0.24
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.05 0 0 0 0 0.05 0.08 0.11 0.12 0.18 0.15 0.18 0 0 0 0 0 0 0.09 0.07 0.1 0.14 0.1 0.08
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0.07 0.06 0.1 0.08
Vac/Vac Membrane 0.51 0.72 0.55 0.42 0.41 0.27 0.25 0.13 0.1 0.1 0 0.05 0.6 0.64 0.53 0.46 0.45 0.5 0 0.12 0.1 0.08 0.07 0.18
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 1 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 4 1 6 4 4 3 2 0 0 1 1 0 0 5 10 10 6 15 18
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 2 1 2 5
Bud Site 0 0 0 0 0 0 1 3 6 6 1 6 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0
Cytoplasm 22 7 26 29 34 31 37 46 20 16 15 10 31 61 80 123 65 49 12 4 18 11 17 23
Endoplasmic Reticulum 0 0 3 0 1 0 1 1 0 0 0 0 1 1 0 18 8 8 0 0 4 0 0 5
Endosome 3 4 17 10 16 9 2 5 2 0 0 0 9 24 14 34 17 10 8 5 17 3 9 7
Golgi 0 1 0 0 1 0 0 0 0 0 0 0 0 0 0 4 1 2 1 1 1 0 1 0
Mitochondria 6 0 5 2 17 70 130 99 99 194 103 142 0 0 0 12 2 3 3 0 3 0 1 1
Nucleus 29 18 38 53 118 163 197 319 171 243 142 166 10 10 9 91 53 40 91 70 124 26 72 42
Nuclear Periphery 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 3 0 1 4
Nucleolus 6 2 3 1 7 17 35 60 35 87 35 56 2 2 1 0 0 0 13 8 27 11 17 14
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 1 0 0 1 0 0 0 0 0 0 0 8 3 19 4 18 14
Vac/Vac Membrane 59 67 95 61 113 91 110 69 31 50 7 16 59 125 98 166 86 84 6 14 28 5 12 32
Unique Cell Count 115 93 172 146 276 336 440 547 298 485 229 305 99 196 184 360 192 168 157 123 274 76 177 179
Labelled Cell Count 125 100 188 156 307 385 515 609 368 600 307 398 112 223 203 449 232 197 157 123 274 76 177 179


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 8.1 10.1 8.8 8.9 8.1 7.5 6.2 6.9 7.2 6.7 6.7 6.7 9.4 9.3 8.9 7.8 8.8 9.6 7.8 9.5 10.4
Std Deviation (1e-4) 1.4 1.5 1.4 1.5 1.5 1.5 1.2 1.5 1.6 1.5 1.3 1.5 1.5 1.5 1.4 1.3 1.5 1.7 1.4 1.7 2.7
Intensity Change (Log2) – – – 0.01 -0.11 -0.23 -0.5 -0.36 -0.29 -0.4 -0.39 -0.4 0.09 0.08 0.01 -0.18 -0.0 0.13 -0.17 0.11 0.25


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 1.1 -0.8 -2.0 -2.5 -2.6 -3.0 -5.4 -2.8 -4.7 3.2 3.6 5.8 4.6 4.1 3.1
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 1.8 1.3 1.6
Endosome -1.0 -1.6 -3.5 -6.0 -6.0 -4.9 0 -4.9 -5.6 -0.2 0.7 -0.8 -0.2 -0.3 -1.3
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 1.5 5.4 7.1 4.9 7.6 9.1 9.4 9.9 0 0 0 0.3 0 0
Nucleus 2.8 4.5 5.8 5.2 8.3 7.4 6.4 8.0 6.9 -2.5 -4.8 -4.8 0.8 1.2 0.4
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 1.8 2.9 3.7 3.8 5.3 4.6 5.3 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole -2.4 -2.9 -6.2 -7.1 -11.6 -10.6 -12.2 -11.9 -12.4 0.7 1.7 -0.4 -2.0 -2.0 -1.0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 4.1624 4.9674 4.3795 4.1509 3.9708 4.4443 4.5438 6.0361 4.9641 4.8059 4.5551 5.311 5.8205 5.7545 5.7599 5.6989 6.0203 5.9605
Actin 0.0201 0.0002 0.0038 0 0.0002 0.0001 0.0321 0.0005 0.0217 0.0052 0.0067 0.0092 0.0023 0.0001 0.0001 0.0003 0.0001 0.0001
Bud 0.0006 0.0001 0.0002 0 0.0004 0.0003 0.0009 0.0001 0.001 0.0004 0.0006 0.0002 0.0002 0.0001 0 0.0001 0.0001 0.0001
Bud Neck 0.0028 0.0009 0.0152 0.0002 0.0018 0.0045 0.0042 0.0007 0.0044 0.0014 0.0042 0.0028 0.0002 0.0005 0.0003 0.0002 0.0008 0.0019
Bud Periphery 0.0007 0.0001 0.0002 0 0.0005 0.0003 0.0016 0 0.0015 0.0007 0.0008 0.0002 0.0002 0 0 0 0 0.0001
Bud Site 0.0023 0.0021 0.0008 0.0001 0.0007 0.0078 0.0122 0.0012 0.0109 0.0003 0.0026 0.0004 0.0007 0.0005 0.0001 0.0002 0.0001 0.0001
Cell Periphery 0.0002 0.0001 0.0001 0 0.0001 0.0002 0.0008 0 0.0004 0.0001 0.0005 0 0 0 0 0 0 0
Cytoplasm 0.0073 0.1359 0.0151 0.0012 0.0125 0.0056 0.0401 0.1565 0.0219 0.016 0.0775 0.0249 0.0297 0.0816 0.0179 0.0381 0.0049 0.024
Cytoplasmic Foci 0.0042 0.0007 0.0024 0 0 0.0009 0.0693 0.001 0.0215 0.0003 0.0175 0.0044 0.0031 0.0002 0.0002 0.0001 0.0001 0.0001
Eisosomes 0.0003 0 0.0001 0 0 0 0.0003 0 0.0001 0 0 0.0001 0.0002 0 0 0 0 0
Endoplasmic Reticulum 0.0029 0.0027 0.0011 0 0.0004 0.0002 0.0104 0.0032 0.0015 0.0031 0.0028 0.0004 0.0012 0.0007 0.0003 0.0004 0.0001 0.0005
Endosome 0.0107 0.0015 0.0069 0 0.0002 0.0004 0.0751 0.0046 0.0198 0.0072 0.0127 0.002 0.0024 0.0004 0.0003 0.0004 0.0002 0.0007
Golgi 0.0039 0 0.0044 0 0 0.0001 0.0359 0 0.0049 0.0015 0.0197 0.0013 0.0009 0 0 0 0 0
Lipid Particles 0.0285 0.0001 0.0016 0 0 0.001 0.0464 0.0001 0.0133 0.0004 0.0082 0.0019 0.0057 0 0 0 0 0
Mitochondria 0.006 0.0002 0.0034 0.0002 0.0007 0.0006 0.0179 0.0002 0.0074 0.0166 0.0031 0.0018 0.0015 0.0002 0.0001 0.0002 0.0001 0.0001
None 0.0008 0.0014 0.0008 0 0.0001 0.0002 0.009 0.0033 0.0012 0.0003 0.0017 0.0002 0.0102 0.0008 0.0114 0.0018 0.0001 0.0002
Nuclear Periphery 0.0131 0.0207 0.0086 0.0035 0.007 0.0037 0.0169 0.0139 0.0056 0.0174 0.0117 0.0044 0.009 0.0088 0.006 0.0043 0.004 0.0051
Nucleolus 0.0059 0.0034 0.0021 0.0016 0.0065 0.0065 0.0031 0.0011 0.0035 0.0012 0.0103 0.002 0.0062 0.0014 0.0019 0.0018 0.0022 0.0027
Nucleus 0.8758 0.8208 0.9298 0.9927 0.9601 0.9626 0.5571 0.8043 0.8275 0.924 0.7695 0.9364 0.909 0.9021 0.9522 0.9487 0.9819 0.9615
Peroxisomes 0.006 0 0.0005 0 0 0.0014 0.0377 0 0.0178 0.0001 0.0269 0.0034 0.0081 0 0 0 0 0
Punctate Nuclear 0.0049 0.0058 0.0008 0.0004 0.0079 0.0024 0.019 0.0058 0.0104 0.0011 0.0116 0.0034 0.0086 0.0018 0.0085 0.003 0.0053 0.0022
Vacuole 0.0026 0.003 0.0013 0.0001 0.0007 0.0008 0.008 0.0031 0.0028 0.001 0.0102 0.0006 0.0004 0.0008 0.0003 0.0001 0.0001 0.0005
Vacuole Periphery 0.0007 0.0002 0.0008 0 0.0002 0.0001 0.0019 0.0003 0.0008 0.0019 0.0011 0.0001 0.0002 0.0001 0.0001 0.0001 0 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 28.9635 26.2035 17.1294 30.8492 25.3366 11.858 21.9209 23.6297 22.5577 32.3553
Translational Efficiency 0.8803 0.7121 1.1446 0.5471 0.7682 1.2376 0.9105 0.6238 0.8064 0.6809

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description High-affinity cyclic AMP phosphodiesterase; component of the cAMP-dependent protein kinase signaling system, protects the cell from extracellular cAMP, contains readthrough motif surrounding termination codon
Localization
Cell Percentages vacuolar membrane (78%)
Cell Cycle Regulation No
Subcompartmental Group vac memb-2

Pde2

Pde2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Pde2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available