Standard name
Human Ortholog
Description Peripheral mitochondrial inner membrane protein; may facilitate use of unformylated tRNA-Met in mitochondrial translation initiation; stabilizes the bicistronic AAP1-ATP6 mRNA
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0.1 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
Bud 0.05 0 0 0.05 0 0 0 0 0 0 0 0 – – – 0.06 0.05 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 – – – – – –
Cell Periphery 0 0.05 0 0 0 0 0 0 0 0 0 0 – – – 0.05 0.06 0 0 0 0 0 0 0
Cytoplasm 0 0.05 0.11 0.12 0.14 0.07 0.09 0.08 0.06 0.06 0.05 0.05 – – – 0.14 0.13 0.15 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0.07 0.07 0.06 0 0 0 0 0.05 0 – – – 0.06 0 0.05 0 0 0 0 0 0
Endosome 0.1 0.18 0.11 0.21 0.19 0.13 0.08 0.07 0 0 0 0 – – – 0.23 0.26 0.31 0.14 0.16 0.1 0 0.15 0
Golgi 0 0.05 0 0 0.05 0 0 0 0 0 0 0 – – – 0 0 0 0 0.07 0 0.08 0.07 0.05
Mitochondria 0.83 0.61 0.28 0.55 0.43 0.68 0.7 0.68 0.84 0.85 0.92 0.91 – – – 0.17 0.07 0.12 0.62 0.59 0.69 0.69 0.55 0.56
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
Peroxisomes 0.1 0.08 0.08 0.05 0.05 0.05 0 0 0 0 0 0 – – – 0.11 0.14 0.18 0.09 0.05 0.05 0 0 0
SpindlePole 0.08 0.05 0.2 0.07 0.05 0.07 0.06 0.16 0.06 0.06 0 0.07 – – – 0.32 0.33 0.34 0 0 0 0 0 0
Vac/Vac Membrane 0.08 0.19 0.18 0 0.14 0.07 0.06 0 0.07 0.07 0 0 – – – 0.12 0.13 0.15 0 0 0 0 0 0.12
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 1 0 6 1 2 0 2 3 0 0 2 0 – – – 4 5 1 1 6 4 0 0 1
Bud 13 3 1 2 1 0 4 4 3 4 7 8 – – – 11 10 7 1 3 0 0 0 0
Bud Neck 0 0 2 0 0 0 1 1 1 1 0 1 – – – 2 0 2 0 2 1 0 0 0
Bud Site 3 0 0 0 0 0 0 1 2 1 1 0 – – – 3 5 2 – – – – – –
Cell Periphery 1 12 2 0 3 0 4 0 1 0 0 2 – – – 9 11 3 0 0 0 0 0 0
Cytoplasm 10 11 7 5 12 7 15 12 8 9 12 12 – – – 26 24 26 1 0 0 0 0 0
Endoplasmic Reticulum 4 5 2 3 6 6 4 5 3 4 13 4 – – – 11 6 9 4 0 0 0 0 0
Endosome 27 40 7 9 17 12 13 11 2 1 1 1 – – – 43 48 55 39 41 14 0 1 1
Golgi 0 11 1 0 4 0 0 1 0 0 0 0 – – – 5 6 5 10 18 2 1 0 1
Mitochondria 228 133 17 23 38 64 111 108 113 126 228 206 – – – 33 14 22 179 150 100 10 7 21
Nucleus 0 1 0 0 0 2 5 5 3 4 2 4 – – – 2 5 6 1 1 0 0 0 0
Nuclear Periphery 1 0 0 0 0 1 1 3 0 3 0 1 – – – 0 0 0 2 0 2 0 0 1
Nucleolus 4 1 0 0 0 2 1 0 1 0 0 1 – – – 0 0 2 1 0 1 0 0 0
Peroxisomes 27 17 5 2 4 5 1 4 1 4 1 2 – – – 20 26 32 26 13 6 0 0 1
SpindlePole 21 12 12 3 4 7 10 26 8 9 5 17 – – – 61 62 60 5 7 2 0 0 0
Vac/Vac Membrane 23 41 11 0 12 7 9 6 9 10 11 9 – – – 23 25 26 6 7 3 0 0 4
Unique Cell Count 276 219 61 42 88 94 158 159 134 149 247 227 190 187 178 289 257 145 15 13 38
Labelled Cell Count 363 287 73 48 103 113 181 190 155 176 283 268 253 247 258 289 257 145 15 13 38


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.5 5.8 6.0 6.0 6.0 5.2 5.2 5.3 4.4 4.6 4.0 4.1 – – – 6.3 6.5 6.6 – – –
Std Deviation (1e-4) 0.7 1.1 0.9 1.8 1.4 1.6 1.4 1.4 1.7 1.3 0.8 1.0 – – – 1.4 1.1 1.6 – – –
Intensity Change (Log2) – – – 0.0 0.01 -0.22 -0.2 -0.17 -0.45 -0.39 -0.58 -0.54 – – – 0.08 0.12 0.13 – – –


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud 0 0 0 0 0 0 0 – – – – – 0 0 0
Bud Neck 0 0 0 0 0 0 0 – – – – – 0 0 0
Bud Site 0 0 0 0 0 0 0 – – – – – 0 0 0
Cell Periphery 0 0 0 0 0 0 0 – – – – – 0 0 0
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum 0 0 0 0 0 0 0 – – – – – 0 0 0
Endosome 1.4 1.3 0.2 -0.7 0 0 0 – – – – – 1.9 2.3 3.0
Golgi 0 0 0 0 0 0 0 – – – – – 0 0 0
Mitochondria 2.8 1.9 4.9 5.7 5.4 0 0 – – – – – -1.8 -4.2 -2.8
Nuclear Periphery 0 0 0 0 0 0 0 – – – – – 0 0 0
Nuc – – – – – – – – – – – – – – –
Nucleolus 0 0 0 0 0 0 0 – – – – – 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – – – – – 0.5 1.2 1.8
SpindlePole -1.8 -2.9 -2.3 -2.9 -0.6 0 0 – – – – – 1.9 2.0 2.1
Vac – – – – – – – – – – – – – – –
Cortical Patches 0 0 0 0 0 0 0 – – – – – 0 0 0
Cytoplasm 0 0.4 -0.9 -0.4 -0.9 0 0 – – – – – 0.4 0.3 0.6
Nucleus 0 0 0 0 0 0 0 – – – – – 0 0 0
Vacuole 0 -0.7 -2.0 -2.8 0 -2.4 -2.5 – – – – – -1.2 -0.9 -0.6

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.9943 1.1312 1.1445 0.9153 0.6758 1.0763 0.8714 0.5615 0.6968 0.7083 0.7142 0.635 0.2533 0.7121 0.5433 0.5316 0.7813 0.3237
Actin 0.0258 0.0128 0.015 0.0019 0.0091 0.0051 0.0675 0.0195 0.0271 0.0083 0.0134 0.012 0.0526 0.0312 0.0789 0.0153 0.0587 0.0612
Bud 0.0029 0.0086 0.0038 0.0006 0.0004 0.0012 0.0017 0.003 0.0015 0.0007 0.002 0.0006 0.0008 0.0015 0.0027 0.0004 0.0005 0.0004
Bud Neck 0.003 0.0024 0.0045 0.0003 0.0002 0.0033 0.0018 0.0009 0.0032 0.0007 0.001 0.0017 0.0083 0.0008 0.0043 0.0006 0.0008 0.0434
Bud Periphery 0.0025 0.0037 0.0057 0.0012 0.001 0.0016 0.0027 0.0069 0.0039 0.0015 0.0069 0.0023 0.0015 0.002 0.0042 0.0016 0.0009 0.0006
Bud Site 0.0084 0.0336 0.0162 0.0016 0.0007 0.0011 0.0129 0.0148 0.0053 0.0015 0.0062 0.0009 0.0241 0.0189 0.011 0.0007 0.0015 0.006
Cell Periphery 0.0007 0.0008 0.0044 0.0003 0.0002 0.0004 0.001 0.0008 0.0032 0.0003 0.0017 0.0005 0.0008 0.0011 0.0023 0.0002 0.0001 0.0003
Cytoplasm 0.0066 0.0138 0.0127 0.0026 0.0008 0.0105 0.017 0.008 0.0141 0.0053 0.0073 0.0044 0.0051 0.0499 0.0226 0.0003 0.0275 0.0074
Cytoplasmic Foci 0.073 0.0642 0.0408 0.0717 0.0209 0.0504 0.0656 0.0833 0.0386 0.0338 0.0365 0.0573 0.0638 0.0897 0.0875 0.0484 0.0531 0.1181
Eisosomes 0.0007 0.0004 0.0022 0.0002 0.0005 0.0003 0.001 0.0007 0.0019 0.0003 0.0007 0.0003 0.0011 0.001 0.0039 0.0003 0.0004 0.0003
Endoplasmic Reticulum 0.0036 0.0008 0.0012 0.0011 0.0033 0.0034 0.004 0.0014 0.0033 0.0003 0.0011 0.0006 0.0016 0.0016 0.0041 0.0001 0.0019 0.0017
Endosome 0.0938 0.0837 0.059 0.0687 0.0981 0.0384 0.0872 0.091 0.0715 0.0369 0.1226 0.0555 0.1492 0.1408 0.0917 0.0124 0.1006 0.0963
Golgi 0.0543 0.069 0.0445 0.0276 0.0437 0.0379 0.062 0.0745 0.0517 0.0517 0.0717 0.053 0.0996 0.1412 0.1064 0.0214 0.0822 0.1136
Lipid Particles 0.0634 0.0262 0.0301 0.0235 0.0248 0.0241 0.0485 0.0176 0.0217 0.026 0.0357 0.0335 0.0823 0.0244 0.0115 0.023 0.1152 0.0717
Mitochondria 0.5438 0.589 0.6422 0.7547 0.7226 0.7107 0.521 0.5643 0.6511 0.7128 0.6417 0.6434 0.4094 0.3665 0.4425 0.6911 0.4309 0.3857
None 0.0084 0.0038 0.0206 0.0013 0.0007 0.0136 0.037 0.0223 0.0248 0.0331 0.0064 0.029 0.0095 0.0597 0.0449 0.0003 0.0233 0.0014
Nuclear Periphery 0.003 0.0003 0.0054 0.0005 0.002 0.0018 0.0019 0.0003 0.0028 0.0002 0.0005 0.0003 0.0006 0.0005 0.0011 0.0001 0.0155 0.0024
Nucleolus 0.0039 0.0005 0.0055 0.0002 0.0003 0.0011 0.0014 0.002 0.0022 0.0004 0.0008 0.0004 0.0007 0.0004 0.0003 0.0001 0.0006 0.0012
Nucleus 0.0032 0.0016 0.0056 0.0003 0.0005 0.0018 0.0018 0.0008 0.001 0.0003 0.0007 0.0003 0.0004 0.0005 0.0006 0.0001 0.0169 0.0011
Peroxisomes 0.0844 0.0756 0.07 0.0321 0.0603 0.0859 0.0484 0.0791 0.0568 0.0805 0.0324 0.0965 0.077 0.0583 0.0681 0.1821 0.0594 0.0796
Punctate Nuclear 0.0034 0.0004 0.0011 0.0003 0.0004 0.0022 0.0065 0.0019 0.0017 0.0003 0.0003 0.0008 0.002 0.0004 0.0006 0.0009 0.0074 0.0033
Vacuole 0.008 0.006 0.0057 0.0056 0.0052 0.0024 0.0057 0.0047 0.0085 0.0028 0.0063 0.0036 0.0054 0.0067 0.0046 0.0004 0.0013 0.0021
Vacuole Periphery 0.0033 0.0028 0.0039 0.0037 0.0042 0.0026 0.0032 0.0023 0.004 0.0024 0.0042 0.0032 0.004 0.0029 0.0062 0.0005 0.0013 0.0023

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 4.8475 3.7917 1.2058 5.2805 3.9281 6.6616 3.423 5.1751 5.6921 5.9919
Translational Efficiency – – – – – – – – – –

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✔
Gene Expression ✘
Translational Efficiency –
Standard name
Human Ortholog
Description Peripheral mitochondrial inner membrane protein; may facilitate use of unformylated tRNA-Met in mitochondrial translation initiation; stabilizes the bicistronic AAP1-ATP6 mRNA
Localization
Cell Percentages mitochondrion (91%)
Cell Cycle Regulation No
Subcompartmental Group mito-1

Aep3

Aep3


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Aep3-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available