| Standard name | |
|---|---|
| Human Ortholog | |
| Description | Zeta subunit of the coatomer complex (COPI); COPI coats Golgi-derived transport vesicles; involved in retrograde transport between Golgi and ER |
Micrographs
Sub-cellular Localization
Yeast GFP Assignment
Protein Abundance
Localization Change
External localization resources
| ensLOC | DeepLoc | |||||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
| Cortical Patches | – | 5 | – | – | – | – | – | – | – | – | – | – | – | – | – | 15 | 4 | 11 | – | 7 | 0 | 0 | 0 | 0 |
| Bud | – | 1 | – | – | – | – | – | – | – | – | – | – | – | – | – | 2 | 1 | 0 | – | 5 | 0 | 0 | 0 | 0 |
| Bud Neck | – | 0 | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 | – | 1 | 0 | 0 | 0 | 0 |
| Bud Site | – | 0 | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 | – | – | – | – | – | – |
| Cell Periphery | – | 2 | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 1 | 0 | – | 0 | 0 | 0 | 0 | 0 |
| Cytoplasm | – | 120 | – | – | – | – | – | – | – | – | – | – | – | – | – | 7 | 5 | 5 | – | 123 | 0 | 0 | 0 | 0 |
| Endoplasmic Reticulum | – | 14 | – | – | – | – | – | – | – | – | – | – | – | – | – | 3 | 1 | 4 | – | 10 | 0 | 0 | 0 | 0 |
| Endosome | – | 0 | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 | – | 6 | 0 | 0 | 0 | 0 |
| Golgi | – | 20 | – | – | – | – | – | – | – | – | – | – | – | – | – | 62 | 27 | 44 | – | 17 | 0 | 0 | 0 | 0 |
| Mitochondria | – | 0 | – | – | – | – | – | – | – | – | – | – | – | – | – | 14 | 4 | 12 | – | 5 | 0 | 0 | 0 | 0 |
| Nucleus | – | 0 | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 | – | 1 | 0 | 0 | 0 | 0 |
| Nuclear Periphery | – | 0 | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 1 | 1 | – | 0 | 0 | 0 | 0 | 0 |
| Nucleolus | – | 0 | – | – | – | – | – | – | – | – | – | – | – | – | – | 1 | 0 | 1 | – | 0 | 0 | 0 | 0 | 0 |
| Peroxisomes | – | 0 | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 |
| SpindlePole | – | 0 | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 | – | 1 | 0 | 0 | 0 | 0 |
| Vac/Vac Membrane | – | 13 | – | – | – | – | – | – | – | – | – | – | – | – | – | 4 | 2 | 8 | – | 34 | 0 | 0 | 0 | 0 |
| Unique Cell Count | 163 | 77 | 37 | 60 | 218 | 1 | 1 | 2 | 1 | |||||||||||||||
| Labelled Cell Count | 175 | 108 | 46 | 86 | 218 | 1 | 1 | 2 | 1 | |||||||||||||||
Yeast GFP Assignment
Protein Abundance
| Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Mean Cell GFP Intensity (1e-4) | – | 15.4 | – | – | – | – | – | – | – | – | – | – | – | – | – | 28.8 | 29.3 | 29.8 | – | – | – |
| Std Deviation (1e-4) | – | 2.1 | – | – | – | – | – | – | – | – | – | – | – | – | – | 8.8 | 10.0 | 10.3 | – | – | – |
| Intensity Change (Log2) | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Localization Change
| Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Bud | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Endosome | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Golgi | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
External localization resources
Images
Protein Concentration and Protein Localization Data
| R1 | R2 | R3 | ||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
| Concentration | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Bud | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Bud Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Cytoplasmic Foci | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Eisosomes | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Endosome | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Golgi | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Lipid Particles | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| None | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Punctate Nuclear | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Vacuole Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Sequencing Data
| R1 | R2 | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
| Gene Expression | 168.4144 | 131.5168 | 137.3981 | 147.8272 | 159.3752 | 129.6266 | 144.3495 | 155.2914 | 140.2384 | 147.5825 |
| Translational Efficiency | 1.4935 | 1.8302 | 1.3932 | 1.3873 | 1.3056 | 1.9806 | 1.6118 | 1.4503 | 1.4685 | 1.5101 |
Hit Data
| Dataset | Hit |
|---|---|
| Protein Concentration | – |
| Protein Localization | – |
| Gene Expression | ✘ |
| Translational Efficiency | ✘ |
Micrographs
Cell Count
| R1 | R2 | R1 & R2 | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
| 24 | 147 | 1525 | 876 | 136 | 149 | 1519 | 164 | 160 | 296 | 3044 | 1040 |
Protein Abundance
| R1 | R2 | R1 & R2 | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
| Mean | 1809.88 | 2015.43 | 1969.61 | 2344.47 | 1357.04 | 1948.85 | 2342.95 | 2328.03 | 1424.97 | 1981.92 | 2155.91 | 2341.88 |
| Standard Deviation | 474.63 | 503.77 | 480.37 | 720.90 | 584.49 | 552.95 | 703.64 | 613.46 | 591.88 | 530.14 | 630.49 | 705.07 |
| Intensity Change Log 2 | — | 0.155194 | 0.122016 | 0.373368 | — | 0.522160 | 0.787863 | 0.778646 | — | 0.323978 | 0.445464 | 0.561114 |
Localization Score (DeepLoc)
| R1 | R2 | R1 & R2 | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
| Actin | 0.012700 | 0.003601 | 0.002078 | 0.012974 | 0.002651 | 0.009085 | 0.003358 | 0.004532 | 0.004158 | 0.006362 | 0.002717 | 0.011643 |
| Bud Neck | 0.000791 | 0.000474 | 0.001607 | 0.001492 | 0.002699 | 0.001365 | 0.000967 | 0.001027 | 0.002413 | 0.000922 | 0.001288 | 0.001419 |
| Bud Site | 0.015522 | 0.010997 | 0.006867 | 0.026148 | 0.049566 | 0.022362 | 0.014543 | 0.010533 | 0.044460 | 0.016718 | 0.010698 | 0.023685 |
| Cell Periphery | 0.003856 | 0.000788 | 0.000388 | 0.006685 | 0.001904 | 0.005865 | 0.000892 | 0.003403 | 0.002197 | 0.003344 | 0.000640 | 0.006167 |
| Cytoplasm | 0.020575 | 0.001258 | 0.004926 | 0.006596 | 0.049603 | 0.005592 | 0.014090 | 0.002956 | 0.045249 | 0.003440 | 0.009499 | 0.006022 |
| Cytoplasmic Foci | 0.031060 | 0.028619 | 0.041465 | 0.031218 | 0.315468* | 0.049914 | 0.033490 | 0.025648 | 0.272807* | 0.039338 | 0.037486 | 0.030339 |
| Eisosomes | 0.000175 | 0.000104 | 0.000033 | 0.000247 | 0.000061 | 0.000086 | 0.000055 | 0.000136 | 0.000078 | 0.000095 | 0.000044 | 0.000229 |
| Endoplasmic Reticulum | 0.030459 | 0.005324 | 0.001515 | 0.006821 | 0.004647 | 0.009963 | 0.001832 | 0.006024 | 0.008519 | 0.007659 | 0.001673 | 0.006695 |
| Endosome | 0.042420 | 0.024422 | 0.085150 | 0.049726 | 0.105251 | 0.044187 | 0.050604 | 0.053919 | 0.095826 | 0.034371 | 0.067911 | 0.050387 |
| Golgi | 0.754252* | 0.883856* | 0.611190* | 0.738542* | 0.378229* | 0.770636* | 0.734233* | 0.808389* | 0.434633* | 0.826863* | 0.672590* | 0.749556* |
| Lipid Particles | 0.005027 | 0.009493 | 0.036471 | 0.020060 | 0.010569 | 0.008592 | 0.010677 | 0.022712 | 0.009738 | 0.009040 | 0.023599 | 0.020478 |
| Mitochondria | 0.023202 | 0.003996 | 0.012464 | 0.018940 | 0.001014 | 0.010745 | 0.012008 | 0.002638 | 0.004342 | 0.007393 | 0.012236 | 0.016370 |
| Mitotic Spindle | 0.004610 | 0.000958 | 0.008277 | 0.003178 | 0.016249 | 0.006658 | 0.011069 | 0.002193 | 0.014503 | 0.003827 | 0.009670 | 0.003023 |
| None | 0.000566 | 0.000107 | 0.001742 | 0.000703 | 0.000171 | 0.000143 | 0.000263 | 0.000137 | 0.000230 | 0.000125 | 0.001004 | 0.000614 |
| Nuclear Periphery | 0.000079 | 0.000044 | 0.002521 | 0.000155 | 0.000339 | 0.000330 | 0.000352 | 0.000161 | 0.000300 | 0.000188 | 0.001439 | 0.000156 |
| Nuclear Periphery Foci | 0.000652 | 0.000166 | 0.008210 | 0.001287 | 0.005486 | 0.005044 | 0.002007 | 0.000900 | 0.004761 | 0.002621 | 0.005115 | 0.001226 |
| Nucleolus | 0.000236 | 0.000004 | 0.000256 | 0.000046 | 0.000832 | 0.000104 | 0.000060 | 0.000039 | 0.000743 | 0.000054 | 0.000158 | 0.000045 |
| Nucleus | 0.000506 | 0.000016 | 0.000211 | 0.000097 | 0.000617 | 0.010619 | 0.000111 | 0.000046 | 0.000600 | 0.005353 | 0.000162 | 0.000089 |
| Peroxisomes | 0.000325 | 0.000158 | 0.000681 | 0.000420 | 0.001826 | 0.000470 | 0.000344 | 0.000301 | 0.001601 | 0.000315 | 0.000513 | 0.000401 |
| Vacuole | 0.018096 | 0.006183 | 0.090825 | 0.038007 | 0.022119 | 0.018365 | 0.052321 | 0.030087 | 0.021515 | 0.012315 | 0.071611 | 0.036758 |
| Vacuole Periphery | 0.034888 | 0.019432 | 0.083120 | 0.036658 | 0.030699 | 0.019874 | 0.056723 | 0.024219 | 0.031327 | 0.019654 | 0.069947 | 0.034697 |
Localization Changes (T-score)
| R1 | R2 | R1 & R2 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
| Actin | 0.87 | 1.02 | -0.05 | -3.61 | -5.18 | -1.95 | -0.70 | -1.05 | 1.36 | -0.65 | -0.86 | 0.72 | -2.98 | -2.18 | -4.92 |
| Bud Neck | 0.87 | -2.01 | -1.84 | -3.92 | 0.19 | 1.52 | 2.02 | 2.00 | 0.77 | 0.18 | 2.12 | 1.56 | 1.36 | -1.96 | -0.54 |
| Bud Site | 0.50 | 1.17 | -1.35 | -2.40 | -7.12 | 2.34 | 3.77 | 4.20 | 1.52 | 1.59 | 3.07 | 4.33 | 2.52 | -1.41 | -5.23 |
| Cell Periphery | 1.71 | 1.96 | -1.27 | -4.60 | -5.05 | -0.81 | 1.68 | -0.99 | 0.49 | -1.75 | -0.45 | 2.77 | -3.28 | -1.07 | -5.12 |
| Cytoplasm | 1.17 | 0.95 | 0.86 | -4.08 | -1.05 | 3.65 | 2.90 | 3.96 | 0.73 | 4.83 | 4.04 | 3.41 | 3.82 | -1.38 | 2.88 |
| Cytoplasmic Foci | 0.16 | -0.79 | -0.02 | -0.40 | 3.84 | 12.14 | 13.89 | 14.03 | 2.71 | 1.73 | 11.80 | 12.31 | 12.68 | 1.74 | 3.36 |
| Eisosomes | 0.65 | 1.31 | -0.63 | -3.43 | -5.80 | -1.08 | 0.34 | -2.49 | -1.37 | -2.64 | -0.75 | 1.78 | -4.21 | -3.88 | -5.84 |
| Endoplasmic Reticulum | 0.98 | 1.13 | 0.92 | -0.75 | -5.19 | -1.04 | 3.39 | -1.04 | 0.75 | -3.63 | 0.17 | 1.75 | 0.45 | 0.34 | -5.68 |
| Endosome | 1.58 | -3.71 | -0.64 | -6.28 | 9.20 | 4.97 | 4.98 | 4.24 | -1.21 | -0.56 | 6.13 | 2.86 | 4.70 | -4.01 | 6.27 |
| Golgi | -2.06 | 2.24 | 0.26 | 7.99 | -10.10 | -10.63 | -12.37 | -13.19 | -1.30 | -4.08 | -12.66 | -8.53 | -11.06 | 4.54 | -7.62 |
| Lipid Particles | -0.88 | -8.37 | -4.45 | -2.22 | 4.05 | 0.37 | -0.04 | -1.75 | -2.14 | -2.02 | 0.16 | -3.73 | -2.73 | -3.35 | 0.94 |
| Mitochondria | 1.21 | 0.67 | 0.26 | -4.54 | -1.95 | -2.40 | -7.41 | -1.35 | 1.92 | 5.04 | -0.94 | -3.05 | -3.47 | -2.72 | -1.49 |
| Mitotic Spindle | 1.22 | -1.15 | 0.33 | -2.86 | 2.85 | 0.99 | 0.54 | 1.52 | 0.82 | 3.52 | 1.36 | 0.63 | 1.47 | 0.17 | 5.01 |
| None | 1.73 | -1.37 | -0.33 | -3.09 | 1.27 | 0.69 | -0.73 | 0.80 | 0.14 | 2.76 | 1.65 | -1.83 | -1.92 | -2.99 | 0.92 |
| Nuclear Periphery | 1.40 | -7.55 | -2.53 | -4.84 | 7.29 | 0.22 | 0.13 | 1.30 | 0.94 | 3.24 | 0.91 | -5.19 | 1.22 | 0.24 | 7.72 |
| Nuclear Periphery Foci | 2.27 | -7.00 | -2.35 | -5.37 | 6.31 | 0.51 | 1.35 | 1.62 | 1.41 | 2.22 | 1.04 | 0.40 | 1.49 | 0.88 | 6.32 |
| Nucleolus | 1.05 | -0.00 | 0.88 | -4.60 | 2.49 | 0.98 | 1.03 | 1.07 | 0.84 | 0.76 | 1.09 | 0.93 | 1.11 | 0.30 | 2.60 |
| Nucleus | 1.07 | 0.67 | 0.90 | -5.31 | 2.08 | -1.32 | 1.53 | 1.81 | 1.42 | 2.99 | -1.23 | 1.59 | 1.86 | 1.40 | 2.91 |
| Peroxisomes | 0.96 | -1.62 | -0.52 | -2.71 | 1.68 | 2.60 | 2.89 | 2.93 | 1.04 | 0.29 | 2.94 | 2.46 | 2.76 | -0.96 | 1.12 |
| Vacuole | 1.91 | -10.19 | -2.91 | -9.54 | 11.51 | 0.75 | -6.91 | -1.16 | -1.73 | 3.50 | 2.43 | -12.81 | -3.69 | -7.58 | 10.06 |
| Vacuole Periphery | 1.20 | -4.12 | -0.15 | -2.54 | 12.02 | 1.29 | -3.27 | 0.72 | -0.80 | 6.29 | 1.54 | -5.62 | -0.52 | -3.62 | 12.09 |
| Standard name | |
|---|---|
| Human Ortholog | |
| Description | Zeta subunit of the coatomer complex (COPI); COPI coats Golgi-derived transport vesicles; involved in retrograde transport between Golgi and ER |
| Localization | |
| Cell Percentages | cytoplasm (38%) |
| Cell Cycle Regulation | No |
| Subcompartmental Group | cytoplasm-5 |
Ret3
Ret3
Nearest neighbours ( genes)
correlation threshold
0.80
Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)
Endocytosis
| Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
|---|---|---|---|---|
| 37℃ | ||||
| RT |
Cell Cycle Omics
CYCLoPs (Ret3-GFP)
| Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
|---|
| Gene | Images |
|---|
| Gene | Images |
|---|
Images are not yet available
Images are not yet available
