Standard name | |
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Human Ortholog | |
Description | Regulatory subunit of the vacuolar transporter chaperone (VTC) complex; involved in membrane trafficking, vacuolar polyphosphate accumulation, microautophagy and non-autophagic vacuolar fusion; VTC3 has a paralog, VTC2, that arose from the whole genome duplication |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 1 | 7 | 1 | 3 | 2 |
Bud | 0 | 0 | 0 | 1 | 2 | 5 | 10 | 8 | – | 11 | 11 | 19 | 0 | 0 | 0 | 0 | 0 | 0 | 10 | 8 | 23 | 7 | 5 | 9 |
Bud Neck | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 2 | – | 0 | 0 | 1 | 0 | 0 | 3 | 1 | 0 | 0 | 2 | 1 | 6 | 5 | 0 | 2 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 1 | 0 | 0 | 1 | 0 | 1 | 0 | 1 | – | 1 | 1 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 |
Cytoplasm | 213 | 148 | 374 | 259 | 278 | 488 | 515 | 728 | – | 599 | 411 | 486 | 73 | 201 | 268 | 199 | 129 | 93 | 116 | 91 | 163 | 99 | 94 | 143 |
Endoplasmic Reticulum | 1 | 1 | 3 | 0 | 2 | 0 | 2 | 0 | – | 0 | 0 | 0 | 0 | 0 | 1 | 12 | 22 | 8 | 1 | 1 | 13 | 1 | 2 | 2 |
Endosome | 7 | 1 | 16 | 1 | 3 | 4 | 1 | 6 | – | 2 | 0 | 1 | 0 | 1 | 0 | 8 | 3 | 4 | 3 | 5 | 14 | 3 | 3 | 5 |
Golgi | 0 | 0 | 2 | 0 | 1 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 1 | 4 | 1 | 3 | 0 | 1 | 0 |
Mitochondria | 1 | 10 | 7 | 11 | 14 | 77 | 113 | 161 | – | 198 | 182 | 224 | 1 | 0 | 0 | 25 | 9 | 3 | 6 | 2 | 32 | 13 | 18 | 8 |
Nucleus | 12 | 3 | 23 | 12 | 20 | 21 | 18 | 28 | – | 6 | 4 | 6 | 1 | 3 | 0 | 72 | 53 | 31 | 1 | 0 | 0 | 0 | 0 | 1 |
Nuclear Periphery | 2 | 0 | 3 | 0 | 3 | 1 | 5 | 9 | – | 7 | 3 | 8 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 1 |
Nucleolus | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 2 | – | 1 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 2 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 1 | 0 | 0 | 1 | 3 | 4 | – | 3 | 1 | 5 | 0 | 0 | 0 | 1 | 0 | 1 | 5 | 1 | 10 | 2 | 7 | 6 |
Vac/Vac Membrane | 62 | 28 | 136 | 75 | 124 | 123 | 127 | 145 | – | 65 | 42 | 58 | 11 | 12 | 6 | 68 | 46 | 39 | 135 | 71 | 267 | 80 | 101 | 99 |
Unique Cell Count | 283 | 182 | 526 | 324 | 397 | 610 | 654 | 932 | 719 | 523 | 625 | 82 | 215 | 274 | 336 | 217 | 155 | 293 | 187 | 560 | 222 | 245 | 292 | |
Labelled Cell Count | 299 | 191 | 566 | 360 | 448 | 721 | 797 | 1094 | 894 | 656 | 809 | 86 | 218 | 281 | 390 | 262 | 180 | 293 | 187 | 560 | 222 | 245 | 292 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 5.6 | 4.7 | 5.0 | 4.7 | 4.6 | 3.5 | 3.3 | 3.5 | – | 3.0 | 2.8 | 2.9 | 5.6 | 5.2 | 4.8 | 5.5 | 5.8 | 6.9 | 5.7 | 5.9 | 5.7 |
Std Deviation (1e-4) | 2.3 | 2.0 | 2.2 | 2.4 | 2.2 | 1.3 | 1.5 | 1.2 | – | 0.9 | 0.8 | 0.9 | 1.6 | 1.3 | 1.1 | 2.0 | 2.2 | 3.1 | 2.2 | 1.8 | 1.9 |
Intensity Change (Log2) | – | – | – | -0.08 | -0.12 | -0.52 | -0.59 | -0.51 | – | -0.76 | -0.82 | -0.78 | 0.15 | 0.06 | -0.06 | 0.12 | 0.2 | 0.45 | 0.19 | 0.23 | 0.19 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | -1.9 | -1.3 | -0.2 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 1.0669 | 0.7819 | 0.9727 | 0.0751 | 0.62 | 0.6562 | 2.2235 | 2.5282 | 2.4284 | 3.009 | 1.8281 | 2.3062 | 2.1664 | 2.5809 | 2.0497 | 2.3408 | 2.2953 | 2.2834 |
Actin | 0.0502 | 0.0045 | 0.0304 | 0.0001 | 0.0235 | 0.0069 | 0.0506 | 0.0001 | 0.0091 | 0.0001 | 0.0003 | 0.0001 | 0.0465 | 0.0001 | 0.0031 | 0.001 | 0.0001 | 0.0006 |
Bud | 0.0035 | 0.0027 | 0.0009 | 0.0003 | 0.0006 | 0.0032 | 0.0023 | 0.0023 | 0.0024 | 0.0002 | 0.0002 | 0.0005 | 0.0007 | 0.0001 | 0.0007 | 0.0006 | 0 | 0.0004 |
Bud Neck | 0.0015 | 0.0017 | 0.0021 | 0.0002 | 0.001 | 0.017 | 0.0038 | 0.0001 | 0.0006 | 0.0011 | 0.0004 | 0.0039 | 0.011 | 0.0001 | 0.0004 | 0.0206 | 0 | 0.0005 |
Bud Periphery | 0.0091 | 0.0018 | 0.0016 | 0.0004 | 0.0008 | 0.0044 | 0.0041 | 0.0018 | 0.0025 | 0.0004 | 0.0002 | 0.0007 | 0.001 | 0.0001 | 0.0026 | 0.0011 | 0 | 0.0006 |
Bud Site | 0.0035 | 0.0154 | 0.0069 | 0.0004 | 0.0061 | 0.0013 | 0.0031 | 0.0116 | 0.0065 | 0.0008 | 0.0002 | 0.0003 | 0.0121 | 0.0003 | 0.0012 | 0.0183 | 0 | 0.0001 |
Cell Periphery | 0.0041 | 0.0019 | 0.0003 | 0.0001 | 0.0002 | 0.0022 | 0.0006 | 0.0003 | 0.0004 | 0.011 | 0.0001 | 0.0008 | 0.0005 | 0.0003 | 0.0016 | 0.0007 | 0.0001 | 0.0011 |
Cytoplasm | 0.0728 | 0.1767 | 0.1096 | 0.2257 | 0.0815 | 0.2221 | 0.249 | 0.246 | 0.0956 | 0.1876 | 0.3012 | 0.2094 | 0.0777 | 0.1762 | 0.1569 | 0.1665 | 0.1548 | 0.2176 |
Cytoplasmic Foci | 0.0301 | 0.0137 | 0.0302 | 0.0476 | 0.0391 | 0.0304 | 0.0358 | 0.0069 | 0.0489 | 0.0473 | 0.0586 | 0.0339 | 0.0178 | 0.0098 | 0.0169 | 0.0303 | 0.0035 | 0.0168 |
Eisosomes | 0.0003 | 0.0001 | 0.0003 | 0.0001 | 0.0004 | 0.0001 | 0.0003 | 0.0003 | 0.0002 | 0.0001 | 0 | 0 | 0.0007 | 0.0002 | 0.0001 | 0.0003 | 0.0001 | 0.0001 |
Endoplasmic Reticulum | 0.009 | 0.0027 | 0.0048 | 0.0048 | 0.0014 | 0.0045 | 0.0499 | 0.0068 | 0.0037 | 0.0073 | 0.003 | 0.0034 | 0.0066 | 0.0041 | 0.0024 | 0.0025 | 0.0016 | 0.0014 |
Endosome | 0.0455 | 0.0125 | 0.048 | 0.1027 | 0.0157 | 0.0351 | 0.1134 | 0.0184 | 0.0944 | 0.1096 | 0.0308 | 0.0475 | 0.0157 | 0.0092 | 0.0272 | 0.0357 | 0.0015 | 0.0119 |
Golgi | 0.0053 | 0.0008 | 0.0083 | 0.0013 | 0.0103 | 0.0053 | 0.0129 | 0.0005 | 0.0157 | 0.0015 | 0.0005 | 0.001 | 0.0111 | 0.0002 | 0.0079 | 0.001 | 0.0001 | 0.0002 |
Lipid Particles | 0.006 | 0.0011 | 0.009 | 0.0046 | 0.0208 | 0.0136 | 0.0044 | 0.0012 | 0.0085 | 0.0045 | 0.0027 | 0.004 | 0.0062 | 0.002 | 0.0024 | 0.0023 | 0.0002 | 0.0006 |
Mitochondria | 0.0028 | 0.0007 | 0.0057 | 0.0018 | 0.0045 | 0.0034 | 0.0054 | 0.0005 | 0.0055 | 0.001 | 0.0004 | 0.0007 | 0.0208 | 0.0004 | 0.0032 | 0.0032 | 0.0001 | 0.0007 |
None | 0.6383 | 0.7136 | 0.6563 | 0.5727 | 0.712 | 0.5817 | 0.3191 | 0.6833 | 0.6788 | 0.4501 | 0.5537 | 0.6291 | 0.7264 | 0.7817 | 0.7498 | 0.6733 | 0.8361 | 0.7231 |
Nuclear Periphery | 0.0247 | 0.0014 | 0.013 | 0.004 | 0.0031 | 0.0033 | 0.046 | 0.0019 | 0.0015 | 0.0036 | 0.0024 | 0.001 | 0.0027 | 0.0009 | 0.0016 | 0.0026 | 0.0002 | 0.0005 |
Nucleolus | 0.0021 | 0.0009 | 0.0031 | 0.0008 | 0.0011 | 0.0022 | 0.0008 | 0.0009 | 0.0008 | 0.0009 | 0.0003 | 0.0005 | 0.0015 | 0.0002 | 0.0005 | 0.0009 | 0 | 0.0002 |
Nucleus | 0.019 | 0.0038 | 0.0216 | 0.0034 | 0.0017 | 0.0065 | 0.0067 | 0.0039 | 0.0011 | 0.0045 | 0.0016 | 0.0015 | 0.0017 | 0.0012 | 0.0013 | 0.0021 | 0.0002 | 0.0012 |
Peroxisomes | 0.0056 | 0.0012 | 0.0082 | 0.0006 | 0.0205 | 0.009 | 0.0012 | 0.0001 | 0.0076 | 0.0005 | 0.0004 | 0.0005 | 0.032 | 0.0001 | 0.0012 | 0.0008 | 0.0001 | 0.0003 |
Punctate Nuclear | 0.0113 | 0.0024 | 0.0152 | 0.0041 | 0.0085 | 0.0039 | 0.0035 | 0.0015 | 0.002 | 0.0012 | 0.0072 | 0.0013 | 0.004 | 0.0003 | 0.0027 | 0.0013 | 0.0001 | 0.0005 |
Vacuole | 0.0491 | 0.039 | 0.0194 | 0.0209 | 0.0461 | 0.0405 | 0.0801 | 0.0108 | 0.012 | 0.1633 | 0.0335 | 0.0572 | 0.0027 | 0.0122 | 0.0149 | 0.029 | 0.0009 | 0.0208 |
Vacuole Periphery | 0.006 | 0.0015 | 0.005 | 0.0034 | 0.0011 | 0.0034 | 0.0067 | 0.0009 | 0.0022 | 0.0037 | 0.0023 | 0.0024 | 0.0006 | 0.0003 | 0.0016 | 0.0058 | 0.0001 | 0.001 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 215.7974 | 239.5148 | 293.5906 | 331.3265 | 244.71 | 197.8697 | 315.4415 | 322.6068 | 326.6392 | 370.7806 |
Translational Efficiency | 0.9941 | 1.2297 | 1.1233 | 0.9827 | 0.9889 | 1.1906 | 1.0286 | 1.0353 | 1.044 | 1.0152 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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