Standard name
Human Ortholog
Description One of two isoforms of the gamma subunit of eEF1B; stimulates the release of GDP from eEF1A (Tef1p/Tef2p) post association with the ribosomal complex with eEF1Balpha subunit; nuclear protein required for transcription of MXR1; binds the MXR1 promoter in the presence of other nuclear factors; binds calcium and phospholipids
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0.06 0 0.06 0.08 0.06 0.07 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0.07 0.09 0.08 0.08 0.08 0.11 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 1.0 0.99 0.93 0.94 0.87 0.91 0.71 0.67 0.68 0.6 0.66 0.62 0.98 0.97 0.97 0.95 0.98 0.97 0.97 0.97 0.89 0.96 0.95 0.94
Endoplasmic Reticulum 0 0 0 0 0.07 0.06 0.17 0.2 0.23 0.18 0.12 0.16 0 0 0 0 0 0 0 0 0.05 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0.07 0.05 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0.08 0.07 0.08 0.2 0.15 0.16 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 1 7 16 11 11 19 7 12 0 1 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 1 0 0 1 0 1 2 2 0 0 0 0 1 0 0 0 0 0 3 1
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 2 7 7 5 3 19 29 16 21 10 19 10 17 19 12 8 7 0 0 1 0 0 0
Cytoplasm 235 164 204 166 156 172 198 215 135 151 82 110 284 409 477 398 319 329 227 151 220 215 336 342
Endoplasmic Reticulum 0 1 9 3 13 12 46 63 45 46 15 28 5 16 18 9 8 15 1 1 11 2 4 12
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 0 0 0 0 0 0 0
Golgi 0 0 2 1 3 1 4 23 10 5 6 7 1 0 2 8 3 5 0 0 1 0 0 1
Mitochondria 2 0 0 0 4 7 23 22 15 51 19 28 0 0 0 0 2 0 0 0 1 0 1 0
Nucleus 0 0 0 0 0 1 1 1 1 0 0 1 1 0 0 0 0 0 0 0 0 1 0 1
Nuclear Periphery 0 0 0 0 0 0 1 1 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 1 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 7 3 6 4 1 3 5 1 3 2 0 2 1 7 1 9 0 0 3 2 4 2
Unique Cell Count 236 165 220 176 179 190 277 320 199 253 124 176 290 423 493 417 326 338 233 156 247 226 354 365
Labelled Cell Count 238 167 229 180 189 207 309 369 238 295 144 210 301 445 517 436 343 365 233 156 247 226 354 365


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 26.7 42.8 33.4 32.3 28.9 26.4 26.0 26.7 25.2 24.5 24.7 22.6 36.8 37.0 34.3 51.6 46.3 52.7 37.4 38.9 39.8
Std Deviation (1e-4) 6.0 8.3 7.1 6.5 6.3 6.6 6.1 6.4 6.1 6.8 13.5 6.4 8.3 7.1 6.8 15.4 13.6 15.4 8.1 9.9 10.3
Intensity Change (Log2) – – – -0.05 -0.21 -0.34 -0.36 -0.33 -0.41 -0.45 -0.44 -0.56 0.14 0.15 0.04 0.63 0.47 0.66 0.16 0.22 0.25


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 3.6 0 3.5 4.1 0 3.9 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0.4 -0.2 0 1.8 2.7 2.2 2.4 2.0 3.0 0.2 0.5 0.4 -0.2 -0.5 -0.8
Cytoplasm 0.6 -1.9 -0.8 -6.0 -7.0 -6.5 -8.3 -6.3 -7.4 2.9 2.3 2.4 1.4 2.9 2.6
Endoplasmic Reticulum -1.4 1.4 1.0 4.4 5.2 5.7 4.8 2.8 4.0 -1.6 -0.2 -0.3 -1.4 -1.1 0.2
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 3.4 2.5 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 4.4 4.0 4.1 7.1 6.0 6.1 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0.1 -0.7 0 0 -0.4 0 0 0 0 0 0 0 0 -0.4

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 26.1302 35.0293 33.6869 30.2773 27.8968 31.8594 21.8937 40.5744 37.2624 25.7483 32.1507 34.6831 21.8688 33.2484 25.5657 26.7562 18.263 29.7566
Actin 0.0029 0.0002 0.0005 0.0006 0.0747 0.0085 0.0389 0.0009 0.0054 0.0058 0.0643 0.0017 0.014 0.0011 0.0053 0.0014 0.0509 0.001
Bud 0.0011 0.0003 0.0008 0.0009 0.0012 0.0011 0.0016 0.0003 0.001 0.0012 0.0018 0.0013 0.0031 0.0159 0.0192 0.0202 0.0006 0.0019
Bud Neck 0.0012 0.0004 0.0005 0.0007 0.0027 0.0016 0.0025 0.0003 0.0004 0.0122 0.0011 0.0014 0.007 0.0006 0.0025 0.0006 0.0003 0.0012
Bud Periphery 0.0019 0.0001 0.0005 0.0008 0.0017 0.0025 0.0019 0.0001 0.0007 0.0016 0.0018 0.0008 0.0025 0.009 0.0127 0.0394 0.0011 0.0069
Bud Site 0.0012 0.0004 0.0003 0.0003 0.0036 0.0003 0.0156 0.0004 0.0044 0.015 0.0009 0.0003 0.0132 0.0152 0.0223 0.003 0.0006 0
Cell Periphery 0.0036 0.0005 0.0008 0.0011 0.0019 0.0016 0.0039 0.0009 0.0031 0.0055 0.0007 0.0004 0.0026 0.0011 0.0014 0.0043 0.0001 0.0007
Cytoplasm 0.7269 0.955 0.9205 0.7922 0.7546 0.8423 0.6106 0.9667 0.9267 0.595 0.6319 0.8819 0.582 0.7629 0.5932 0.5792 0.4353 0.7931
Cytoplasmic Foci 0.0119 0.0031 0.004 0.006 0.0163 0.0083 0.0613 0.0016 0.004 0.0282 0.0064 0.0083 0.0441 0.0416 0.054 0.1429 0.0474 0.0456
Eisosomes 0.0002 0 0 0 0.0004 0.0001 0.0007 0 0 0.0003 0.0008 0 0.0001 0 0.0001 0.0001 0.0004 0
Endoplasmic Reticulum 0.0827 0.0173 0.0193 0.057 0.0075 0.0598 0.0508 0.0075 0.022 0.0318 0.0082 0.0421 0.0569 0.0083 0.028 0.0063 0.0064 0.0218
Endosome 0.0287 0.0073 0.0133 0.0223 0.0441 0.0296 0.0404 0.0027 0.0061 0.0935 0.0156 0.0257 0.0481 0.0087 0.018 0.0105 0.0932 0.0141
Golgi 0.0053 0.001 0.0013 0.0008 0.016 0.0068 0.013 0.0006 0.0023 0.0088 0.0059 0.0031 0.0086 0.0006 0.0042 0.0005 0.0223 0.0036
Lipid Particles 0.006 0.0002 0.0002 0.0011 0.0049 0.0009 0.029 0.0002 0.0005 0.0176 0.0023 0.0002 0.0118 0.0035 0.014 0.0017 0.0214 0.0007
Mitochondria 0.0266 0.0005 0.0013 0.0453 0.0116 0.0088 0.0063 0.0003 0.0019 0.0469 0.1124 0.0037 0.0049 0.0005 0.0056 0.002 0.0324 0.0032
None 0.0038 0.0002 0.0003 0.0022 0.0012 0.0006 0.013 0.0003 0.0003 0.0033 0.0007 0.0005 0.1128 0.0988 0.1554 0.0275 0.2464 0.0863
Nuclear Periphery 0.0138 0.0011 0.0045 0.0061 0.0031 0.0047 0.0264 0.0017 0.002 0.015 0.0046 0.0027 0.0115 0.0015 0.0029 0.0076 0.0113 0.0043
Nucleolus 0.0016 0 0.0001 0.0001 0.0008 0.0001 0.0038 0 0 0.0019 0.0002 0.0001 0.0009 0.0059 0.0015 0.0015 0.0002 0.0001
Nucleus 0.0094 0.0007 0.0015 0.0027 0.0028 0.0016 0.0102 0.0009 0.0012 0.0045 0.0051 0.0023 0.0067 0.0021 0.0052 0.0049 0.0013 0.0015
Peroxisomes 0.0023 0 0 0.0005 0.0017 0.0031 0.0141 0 0.0004 0.0018 0.0015 0.0001 0.0031 0.003 0.0212 0.0043 0.0144 0.0006
Punctate Nuclear 0.0052 0 0 0.0002 0.0034 0.0001 0.0081 0.0001 0.0001 0.0032 0.0002 0.0002 0.0022 0.0016 0.0033 0.0019 0.0015 0.0004
Vacuole 0.0494 0.0102 0.0254 0.0503 0.036 0.0127 0.037 0.0138 0.0152 0.0884 0.1226 0.0177 0.0525 0.016 0.0252 0.1194 0.0063 0.0101
Vacuole Periphery 0.0143 0.0015 0.005 0.0086 0.0098 0.0049 0.0108 0.0007 0.0023 0.0183 0.0109 0.0054 0.0112 0.0021 0.0049 0.0209 0.0064 0.0029

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 328.4573 338.2764 329.0183 282.0631 315.239 338.4375 295.2814 279.6603 324.0568 269.0577
Translational Efficiency 1.4526 1.4185 1.356 1.77 1.5672 1.3608 1.3764 1.5823 1.3539 1.357

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description One of two isoforms of the gamma subunit of eEF1B; stimulates the release of GDP from eEF1A (Tef1p/Tef2p) post association with the ribosomal complex with eEF1Balpha subunit; nuclear protein required for transcription of MXR1; binds the MXR1 promoter in the presence of other nuclear factors; binds calcium and phospholipids
Localization
Cell Percentages cytoplasm (100%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-2

Cam1

Cam1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Cam1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available