Standard name
Human Ortholog
Description Component of the ESCRT-I complex; complex is involved in ubiquitin-dependent sorting of proteins into the endosome; conserved C-terminal domain interacts with ESCRT-III subunit Vps20p; other members include Stp22p, Srn2p, Vps28p, and Mvb12p
DOWNLOAD

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches – – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud – – 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck – – 0 0 0 0 0 0 0 0 0 0 0 0.06 0.05 0 0 0 0 0 0 0 0 0
Bud Site – – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery – – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm – – 0.84 0.8 0.91 0.85 0.86 0.87 0.71 0.69 0.33 0.47 0.84 0.88 0.88 0.64 0.48 0.43 0.26 0.46 0.38 0.57 0.46 0.34
Endoplasmic Reticulum – – 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0 0 0 0 0 0 0 0
Endosome – – 0 0 0 0 0 0 0 0 0 0 0 0 0 0.24 0.42 0.43 0.33 0.2 0.2 0.12 0.18 0.28
Golgi – – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria – – 0 0.11 0 0.08 0.08 0.13 0.37 0.34 0.7 0.57 0.05 0 0 0 0.06 0.05 0.1 0.08 0.21 0.11 0.1 0.15
Nucleus – – 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery – – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus – – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.1 0 0 0 0 0
Peroxisomes – – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole – – 0.06 0.09 0 0 0.06 0 0 0 0 0 0.06 0.07 0.1 0.16 0.16 0.12 0 0 0.05 0.05 0.08 0.07
Vac/Vac Membrane – – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0.13 0.12 0.19 0 0.07 0.07 0.06
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches – – 0 3 1 2 0 0 0 1 0 0 2 0 0 0 4 4 0 0 1 1 2 4
Bud – – 0 3 1 1 1 1 4 5 2 7 2 0 3 1 3 2 0 0 1 0 2 3
Bud Neck – – 1 0 3 1 3 1 4 1 2 2 1 14 17 0 0 1 0 0 0 0 2 2
Bud Site – – 0 1 0 0 0 0 1 0 2 0 1 0 0 0 1 1 – – – – – –
Cell Periphery – – 0 0 0 0 3 0 0 0 0 2 1 2 4 0 0 0 0 0 0 0 0 0
Cytoplasm – – 42 64 63 92 131 148 87 89 21 64 69 200 280 16 69 68 3 20 31 57 108 97
Endoplasmic Reticulum – – 0 1 0 1 1 0 1 0 1 0 1 2 0 2 6 7 0 0 2 1 1 3
Endosome – – 1 2 0 2 0 0 0 0 0 3 0 1 3 6 61 68 4 8 16 12 42 81
Golgi – – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 1
Mitochondria – – 2 9 2 9 12 22 45 44 44 78 4 2 1 1 8 8 1 3 17 10 22 43
Nucleus – – 0 1 0 2 1 1 2 0 3 4 0 0 0 0 2 0 0 0 0 0 3 0
Nuclear Periphery – – 0 0 0 0 0 0 0 1 0 1 0 0 0 0 0 0 0 0 0 0 0 1
Nucleolus – – 1 0 0 0 0 0 1 1 0 0 0 0 0 0 0 0 1 0 0 0 0 0
Peroxisomes – – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 5 0 0 0 0 0 0
SpindlePole – – 3 7 1 2 9 5 2 4 1 4 5 17 31 4 23 19 0 0 4 5 17 19
Vac/Vac Membrane – – 1 0 2 1 2 5 0 1 2 3 1 0 0 1 12 20 1 8 1 7 17 16
Unique Cell Count 50 80 69 108 153 171 123 129 63 136 82 228 318 25 144 158 12 44 83 100 237 286
Labelled Cell Count 51 91 73 113 163 183 147 147 78 168 87 238 339 31 190 203 12 44 83 100 237 286


Punctate Nuclear

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) – – 4.8 4.5 5.0 4.3 4.3 4.6 3.8 3.9 3.7 4.1 4.2 4.7 4.6 5.6 6.2 7.0 4.8 5.7 5.6
Std Deviation (1e-4) – – 1.0 1.1 0.9 0.9 1.2 2.7 1.2 1.3 0.8 1.7 0.4 0.9 0.9 2.5 1.6 2.0 0.6 1.5 1.5
Intensity Change (Log2) – – – -0.07 0.07 -0.15 -0.16 -0.04 -0.34 -0.28 -0.36 -0.2 -0.19 -0.03 -0.06 0.24 0.38 0.56 0.02 0.26 0.24


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -0.6 1.2 0.2 0.3 0.5 -1.8 -2.0 -5.4 -4.5 0 0.7 0.8 -2.0 -4.4 -5.1
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 5.3 5.4
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 1.8 1.2
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2.2

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -0.9199 -0.6375 -0.7342 -1.1376 -0.5639 -0.879 2.5811 1.082 0.8868 0.4413 1.5758 0.7481 -1.1535 -0.8191 -1.2512 -1.1282 -1.8993 -1.11
Actin 0.0237 0.0042 0.0018 0.0032 0.062 0.002 0.2272 0.0002 0.0001 0.0004 0.0008 0.0007 0.0381 0.0001 0.001 0 0 0.0151
Bud 0.0003 0.0003 0.0008 0.0001 0.0003 0.0001 0.0038 0.0001 0.0001 0.0008 0.0001 0.0006 0.0002 0.0002 0.0002 0.0001 0.0001 0.0018
Bud Neck 0.0007 0.0006 0.0006 0.0019 0.0007 0.0008 0.0086 0.0001 0.0001 0.0005 0.0002 0.0004 0.0011 0.0003 0.0004 0.0001 0.0013 0.0016
Bud Periphery 0.0005 0.0003 0.0018 0.0001 0.0007 0.0001 0.0058 0.0001 0.0001 0.0016 0.0001 0.0016 0.0004 0.0001 0.0003 0.0001 0 0.0021
Bud Site 0.0011 0.0021 0.0025 0.0005 0.001 0.0002 0.0859 0.0008 0.0003 0.0005 0.0004 0.0007 0.0014 0.0273 0.0007 0.0001 0.0001 0.0005
Cell Periphery 0.0003 0.0002 0.0002 0 0.0003 0.0001 0.0038 0.0001 0.0001 0.0003 0 0.0001 0.0004 0.0002 0.0001 0.0001 0.0001 0.0002
Cytoplasm 0.09 0.0844 0.091 0.0601 0.0278 0.096 0.0211 0.1202 0.1008 0.1275 0.0468 0.0612 0.1073 0.1336 0.1548 0.1878 0.0942 0.2152
Cytoplasmic Foci 0.3016 0.2902 0.2653 0.3311 0.3283 0.3312 0.1413 0.1981 0.1694 0.1404 0.1247 0.317 0.1918 0.2153 0.222 0.1816 0.3642 0.2497
Eisosomes 0.0007 0.0005 0.0002 0.0001 0.0006 0.0001 0.0319 0.0002 0.0004 0.0001 0.0001 0.0001 0.0008 0 0.0016 0 0 0.0001
Endoplasmic Reticulum 0.0065 0.0019 0.0043 0.0012 0.0033 0.0019 0.0043 0.0017 0.0024 0.0035 0.0009 0.0022 0.0065 0.0047 0.0029 0.0018 0.0009 0.0035
Endosome 0.2249 0.2284 0.2727 0.184 0.2474 0.2656 0.1854 0.1433 0.1199 0.055 0.2188 0.0992 0.1923 0.2998 0.2625 0.3206 0.3434 0.2365
Golgi 0.0131 0.0089 0.0171 0.0123 0.0177 0.0121 0.0147 0.0053 0.004 0.0035 0.0076 0.0092 0.0099 0.0083 0.0087 0.0058 0.0065 0.0161
Lipid Particles 0.0396 0.037 0.0439 0.0221 0.0414 0.0267 0.0948 0.0124 0.0324 0.0329 0.0735 0.0308 0.0648 0.0235 0.0315 0.0124 0.0213 0.0284
Mitochondria 0.0046 0.0018 0.0091 0.0059 0.0091 0.0027 0.016 0.0005 0.001 0.0036 0.0064 0.0206 0.0114 0.0005 0.0028 0.001 0.0014 0.0095
None 0.236 0.2931 0.2431 0.2812 0.189 0.2157 0.0755 0.495 0.5538 0.6009 0.334 0.3919 0.3104 0.2585 0.285 0.2561 0.1266 0.1914
Nuclear Periphery 0.0038 0.0011 0.002 0.0008 0.0149 0.0039 0.0049 0.0009 0.0011 0.0014 0.0005 0.0009 0.0127 0.0024 0.0024 0.0011 0.0005 0.0022
Nucleolus 0.0006 0.0008 0.0016 0.0003 0.0032 0.0004 0.0035 0.0004 0.0002 0.0005 0.0003 0.0006 0.001 0.0008 0.0006 0.0004 0.0011 0.0004
Nucleus 0.0011 0.0013 0.0018 0.0009 0.0029 0.0013 0.006 0.0005 0.0005 0.0012 0.0003 0.0006 0.0193 0.0013 0.0015 0.0009 0.0011 0.001
Peroxisomes 0.0233 0.0285 0.0152 0.0775 0.0313 0.024 0.031 0.0095 0.006 0.0128 0.1572 0.0411 0.0094 0.004 0.0049 0.0014 0.0058 0.0091
Punctate Nuclear 0.0044 0.004 0.0058 0.0079 0.0097 0.0049 0.0298 0.0011 0.001 0.0027 0.0025 0.0036 0.0044 0.0014 0.0022 0.0013 0.0035 0.0035
Vacuole 0.0197 0.009 0.0161 0.0077 0.0047 0.0084 0.0036 0.009 0.0055 0.0081 0.0203 0.0153 0.0144 0.0161 0.0119 0.0241 0.0251 0.0107
Vacuole Periphery 0.0034 0.0014 0.0029 0.0012 0.0037 0.0019 0.0011 0.0006 0.0009 0.0019 0.0046 0.0016 0.002 0.0014 0.0019 0.0034 0.0027 0.0017

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 8.3247 8.1183 6.0241 13.8702 12.7558 6.051 10.9446 14.7737 11.3266 11.7486
Translational Efficiency 1.5445 1.7911 2.2815 0.8416 0.9408 1.8012 1.7876 1.0297 1.3683 1.387

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
66 229 1494 7 1227 1268 147 8 1293 1497 1641 15

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 603.50 784.89 750.17 836.14 617.91 736.22 925.74 777.08 617.17 743.67 765.90 804.64
Standard Deviation 70.20 84.08 95.95 122.27 75.00 94.21 133.76 94.63 74.83 94.37 111.80 112.34
Intensity Change Log 2 — 0.379137 0.313864 0.470391 — 0.252740 0.583210 0.330666 — 0.316577 0.456397 0.401396

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000994 0.001640 0.001246 0.000592 0.000292 0.000392 0.002310 0.000492 0.000328 0.000583 0.001341 0.000539
Bud Neck 0.033147 0.018557 0.031130 0.058662 0.028536 0.033014 0.013899 0.005951 0.028771 0.030803 0.029586 0.030550
Bud Site 0.035125 0.014362 0.028389 0.044690 0.009093 0.011506 0.025994 0.225374* 0.010421 0.011943 0.028175 0.141055*
Cell Periphery 0.001457 0.000261 0.000124 0.000144 0.000260 0.000232 0.000208 0.000284 0.000321 0.000237 0.000132 0.000219
Cytoplasm 0.357927* 0.235896* 0.142270* 0.031717 0.341389* 0.296562* 0.213648* 0.294762* 0.342233* 0.287282* 0.148664* 0.172008*
Cytoplasmic Foci 0.267741* 0.399570* 0.191170* 0.103358 0.357690* 0.307628* 0.059886 0.120241 0.353098* 0.321693* 0.179409 0.112362
Eisosomes 0.000534 0.000274 0.000089 0.000145 0.000294 0.000211 0.000104 0.000075 0.000307 0.000220 0.000090 0.000108
Endoplasmic Reticulum 0.001189 0.003259 0.003647 0.001017 0.002386 0.001066 0.006528 0.000320 0.002325 0.001402 0.003905 0.000645
Endosome 0.016764 0.066264 0.229505* 0.087967 0.030077 0.062214 0.082021 0.081678 0.029397 0.062833 0.216293* 0.084613
Golgi 0.013927 0.017009 0.008395 0.013527 0.009354 0.009709 0.005423 0.003143 0.009588 0.010826 0.008129 0.007989
Lipid Particles 0.044861 0.017545 0.021551 0.002370 0.029219 0.023101 0.012512 0.006573 0.030018 0.022252 0.020741 0.004611
Mitochondria 0.097767 0.012447 0.006902 0.000778 0.015020 0.022924 0.004688 0.002808 0.019244 0.021321 0.006704 0.001861
Mitotic Spindle 0.001852 0.004969 0.054882 0.005675 0.000584 0.003839 0.052626 0.011289 0.000649 0.004012 0.054680 0.008669
None 0.006438 0.001553 0.001198 0.000025 0.002603 0.002387 0.002644 0.047548 0.002799 0.002260 0.001328 0.025371
Nuclear Periphery 0.000704 0.000668 0.003236 0.000573 0.000493 0.000708 0.007185 0.000449 0.000504 0.000702 0.003590 0.000507
Nuclear Periphery Foci 0.000325 0.001084 0.007066 0.001269 0.000458 0.000474 0.011539 0.000206 0.000451 0.000568 0.007467 0.000702
Nucleolus 0.001132 0.001853 0.006893 0.001692 0.001299 0.002507 0.005303 0.003551 0.001290 0.002407 0.006751 0.002683
Nucleus 0.067395 0.078077 0.081565 0.030241 0.115180 0.098259 0.129949 0.088377 0.112741 0.095172 0.085899 0.061247
Peroxisomes 0.026032 0.031457 0.048775 0.049044 0.016074 0.024265 0.019946 0.004973 0.016582 0.025365 0.046192 0.025540
Vacuole 0.019588 0.088389 0.129634 0.564050* 0.037533 0.094367 0.333253* 0.096046 0.036617 0.093453 0.147874 0.314448*
Vacuole Periphery 0.005100 0.004865 0.002333 0.002465 0.002165 0.004632 0.010335 0.005858 0.002315 0.004668 0.003050 0.004275

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN -2.29 -5.46 -0.84 0.30 2.68
Bud Neck NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN -0.64 -1.00 0.38 0.51 0.58
Bud Site NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN -0.81 -6.21 -1.65 -1.63 -1.43
Cell Periphery NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN 2.26 6.07 1.85 0.59 -1.28
Cytoplasm NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN 5.66 21.66 2.67 1.81 -0.38
Cytoplasmic Foci NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN 3.50 22.92 7.63 6.71 2.22
Eisosomes NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN 4.09 10.81 7.40 5.46 -0.37
Endoplasmic Reticulum NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN 3.39 -5.93 6.02 3.92 12.21
Endosome NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN -8.84 -25.13 -1.10 -0.24 3.13
Golgi NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN -0.92 2.62 0.85 1.11 0.21
Lipid Particles NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN 3.40 3.89 9.01 7.22 6.83
Mitochondria NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN -0.74 6.40 7.83 9.26 3.72
Mitotic Spindle NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN -3.58 -15.72 -1.34 -0.65 8.00
None NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN 0.80 3.26 -0.91 -0.93 -0.96
Nuclear Periphery NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN -2.79 -9.94 -0.09 1.34 9.46
Nuclear Periphery Foci NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN -1.13 -10.01 -1.44 -0.80 9.32
Nucleolus NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN -2.85 -9.85 -0.72 -0.04 3.74
Nucleus NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN 3.06 2.36 2.31 1.60 1.69
Peroxisomes NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN -4.32 -10.27 -0.24 0.53 1.96
Vacuole NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN -14.23 -23.70 -3.33 -2.76 -2.05
Vacuole Periphery NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN -4.42 -0.14 -0.51 0.35 -0.49
Standard name
Human Ortholog
Description Component of the ESCRT-I complex; complex is involved in ubiquitin-dependent sorting of proteins into the endosome; conserved C-terminal domain interacts with ESCRT-III subunit Vps20p; other members include Stp22p, Srn2p, Vps28p, and Mvb12p
Localization
Cell Percentages cytoplasm (10%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Vps28

Vps28


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Vps28-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available