Standard name
Human Ortholog
Description Regulator of Rho1p signaling, cofactor of Tus1p; required for the localization of Tus1p during all phases of cytokinesis; green fluorescent protein (GFP)-fusion protein localizes to the bud neck and cytoplasm; null mutant is viable and exhibits growth defect on a non-fermentable (respiratory) carbon source
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.06 0.07 0 0.08 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0.08 0.06 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.07 0 0 0 0 0 0
Cytoplasm 0.89 0.89 0.92 0.86 0.86 0.81 0.75 0.72 0.51 0.57 0.46 0.44 0.99 1.0 1.0 0.95 0.94 0.94 0.76 0.78 0.74 0.91 0.93 0.84
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.07 0.07 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.06 0 0 0.09 0.09 0.28 0.37 0.35 0.63 0.59 0.69 0.69 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0.05 0.06 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 8 14 2 8 3 3 1 1 3 1 0 2 2 0 0 6 1 5 4 2 2 0 0 4
Bud 5 3 1 2 0 1 4 1 2 5 6 8 0 1 0 1 0 0 5 4 1 0 1 7
Bud Neck 0 0 0 0 0 2 2 2 0 0 1 0 0 0 0 5 1 1 13 16 5 0 0 3
Bud Site 3 6 2 0 1 1 0 0 3 5 0 1 1 0 0 2 1 0 – – – – – –
Cell Periphery 0 0 0 4 0 4 3 1 5 2 6 4 4 6 11 9 22 25 0 0 0 0 0 1
Cytoplasm 116 174 67 89 96 136 141 176 106 112 64 87 224 485 405 631 391 338 139 164 72 78 193 242
Endoplasmic Reticulum 0 4 0 2 1 2 0 5 2 3 1 7 0 2 1 36 31 24 1 0 3 0 0 2
Endosome 2 0 0 0 1 0 0 4 0 1 0 0 1 0 0 7 5 3 3 4 1 1 2 3
Golgi 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 2 4 0 0 0 0 1 2
Mitochondria 8 1 2 9 10 47 69 85 129 116 97 135 0 1 0 6 3 2 3 3 1 2 3 9
Nucleus 1 0 0 1 0 1 0 0 0 0 1 0 0 1 2 2 1 2 0 0 0 0 0 0
Nuclear Periphery 0 1 0 1 0 0 0 0 0 1 1 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 2 1 1 0 4 1 1 0 0 0 1 0 0 1 0 0 0 0 0
Peroxisomes 2 5 4 6 2 9 0 3 3 4 0 2 0 0 0 2 4 1 0 0 0 0 0 0
SpindlePole 1 1 3 0 0 0 2 4 3 6 1 2 0 0 0 15 12 8 4 5 6 0 0 1
Vac/Vac Membrane 0 1 0 0 1 0 3 3 0 1 1 5 0 0 0 5 7 3 0 3 0 0 2 6
Unique Cell Count 130 195 73 103 111 168 188 245 206 196 140 196 227 487 407 665 416 359 182 211 98 86 209 290
Labelled Cell Count 146 210 81 122 116 208 226 286 256 261 180 254 232 496 419 728 481 416 182 211 98 86 209 290


Bud Neck, Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.6 5.6 5.2 4.5 4.7 4.1 3.8 4.1 4.0 4.0 3.8 3.6 5.9 6.1 5.9 6.0 7.5 7.5 4.6 5.4 6.1
Std Deviation (1e-4) 0.5 0.7 1.9 0.7 0.9 1.4 1.1 1.0 1.2 1.5 0.8 0.8 1.0 1.8 1.5 1.4 1.5 1.7 1.0 1.5 1.3
Intensity Change (Log2) – – – -0.22 -0.16 -0.34 -0.45 -0.36 -0.4 -0.4 -0.48 -0.54 0.17 0.22 0.17 0.21 0.53 0.52 -0.19 0.04 0.23


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -1.1 -1.1 -2.1 -3.0 -3.5 -6.1 -5.4 -6.6 -7.0 3.0 5.2 4.7 1.1 0.7 0.8
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.204 1.8558 1.704 1.3845 1.5756 1.737 3.8698 4.034 4.0923 4.2172 3.7767 4.0736 3.9612 3.704 4.4539 4.4068 4.5222 3.7762
Actin 0.0756 0.0053 0.0131 0.0003 0.044 0.0129 0.0105 0.0035 0.0094 0.0075 0.0004 0.0148 0.0094 0.0002 0.0165 0.0013 0.0001 0.0042
Bud 0.0029 0.0015 0.001 0.0004 0.0002 0.0012 0.0021 0.0049 0.0028 0.0013 0.0001 0.0026 0.0008 0 0.0013 0 0 0.0001
Bud Neck 0.0214 0.0092 0.0007 0.0003 0.0005 0.1678 0.0159 0.0011 0.0007 0.0129 0.0001 0.1792 0.0005 0 0.0081 0.0001 0 0.1356
Bud Periphery 0.004 0.002 0.0011 0.0007 0.0002 0.0011 0.0033 0.0075 0.0044 0.0027 0.0001 0.0072 0.0033 0 0.0018 0 0 0.0001
Bud Site 0.0163 0.0268 0.0038 0.0004 0.0003 0.0027 0.0261 0.0395 0.0061 0.0138 0.0001 0.0035 0.0009 0.0002 0.0137 0 0 0.0003
Cell Periphery 0.0015 0.0007 0.0003 0.0003 0.0003 0.0004 0.0016 0.0005 0.0003 0.0007 0.0001 0.0009 0.0004 0.0002 0.0008 0.0001 0 0.0002
Cytoplasm 0.2451 0.3992 0.3823 0.366 0.4614 0.3491 0.2971 0.4126 0.3766 0.3314 0.3328 0.4313 0.2246 0.4672 0.1997 0.4324 0.3514 0.3954
Cytoplasmic Foci 0.0485 0.0128 0.0109 0.0454 0.0239 0.012 0.0343 0.0122 0.0129 0.0049 0.004 0.0058 0.0521 0.0031 0.0233 0.0035 0.0036 0.0099
Eisosomes 0.0007 0.0002 0.0004 0.0001 0.0003 0.0001 0.0004 0.0001 0.0001 0.0002 0.0001 0.0002 0.0002 0.0001 0.0005 0.0001 0 0
Endoplasmic Reticulum 0.0096 0.0043 0.0062 0.0037 0.0026 0.0016 0.0034 0.0015 0.0017 0.0048 0.0016 0.0011 0.0053 0.0021 0.0035 0.0025 0.0004 0.0025
Endosome 0.0275 0.0058 0.0069 0.0235 0.0176 0.0012 0.0175 0.0023 0.0029 0.0028 0.0015 0.0012 0.0271 0.0008 0.0099 0.0008 0.0007 0.0065
Golgi 0.0092 0.0013 0.0012 0.0014 0.0029 0.0003 0.0067 0.0006 0.0019 0.0007 0.0001 0.0012 0.0031 0.0001 0.017 0.0001 0 0.0045
Lipid Particles 0.0096 0.0016 0.0019 0.0029 0.0043 0.0003 0.0072 0.0011 0.0015 0.0005 0.0001 0.0007 0.0058 0.0002 0.0282 0.0002 0.0002 0.0021
Mitochondria 0.0028 0.0033 0.0006 0.0045 0.0009 0.0003 0.0034 0.0099 0.0083 0.0005 0.0002 0.0053 0.0099 0.0001 0.0028 0.0001 0 0.0011
None 0.4905 0.5189 0.5532 0.5382 0.4173 0.4453 0.5473 0.493 0.5483 0.6009 0.6567 0.3339 0.4637 0.524 0.6615 0.5571 0.6419 0.4322
Nuclear Periphery 0.0122 0.0008 0.0045 0.001 0.0014 0.0003 0.0018 0.0027 0.0011 0.002 0.0004 0.0025 0.0782 0.0003 0.0006 0.0004 0.0001 0.0003
Nucleolus 0.0008 0.0002 0.0004 0.0003 0.0003 0.0001 0.0019 0.0006 0.0001 0.0006 0.0001 0.0001 0.002 0.0001 0.0003 0 0 0
Nucleus 0.0035 0.0012 0.0034 0.0013 0.0011 0.0009 0.0014 0.0017 0.0054 0.0035 0.0005 0.0015 0.037 0.0005 0.0007 0.0004 0.0003 0.0004
Peroxisomes 0.008 0.0019 0.0009 0.0008 0.0091 0.0006 0.0102 0.0005 0.0135 0.0004 0.0001 0.0053 0.0181 0 0.0074 0.0001 0.0001 0.0039
Punctate Nuclear 0.0073 0.0011 0.0053 0.0013 0.0097 0.0008 0.0052 0.0026 0.001 0.001 0.0004 0.0012 0.0078 0.0002 0.001 0.0003 0.0002 0.0004
Vacuole 0.002 0.0017 0.0016 0.0064 0.0013 0.0008 0.002 0.0014 0.001 0.0065 0.0005 0.0004 0.0157 0.0006 0.0011 0.0003 0.0005 0.0004
Vacuole Periphery 0.0009 0.0002 0.0003 0.0008 0.0005 0.0001 0.0005 0.0003 0.0001 0.0004 0.0001 0.0002 0.034 0 0.0002 0 0.0001 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 10.3444 13.0147 13.7238 23.5436 12.9152 11.4874 15.2369 14.0235 15.9823 22.7725
Translational Efficiency 0.4929 0.352 0.28 0.1754 0.3026 0.4065 0.2588 0.3764 0.239 0.2257

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Regulator of Rho1p signaling, cofactor of Tus1p; required for the localization of Tus1p during all phases of cytokinesis; green fluorescent protein (GFP)-fusion protein localizes to the bud neck and cytoplasm; null mutant is viable and exhibits growth defect on a non-fermentable (respiratory) carbon source
Localization
Cell Percentages cytoplasm (82%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Rgl1

Rgl1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Rgl1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available