Standard name
Human Ortholog
Description MADS-box transcription factor; component of the protein kinase C-mediated MAP kinase pathway involved in the maintenance of cell integrity; phosphorylated and activated by the MAP-kinase Slt2p; RLM1 has a paralog, SMP1, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 – – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Cytoplasm 0 0 0.08 0.12 0.08 0.07 0.07 – 0.1 0.06 0.05 0 0.15 0.16 0.24 0.12 0.15 – 0 0 0 0.05 0 0.07
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0.1 0.06 – 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0.12 0.14 – 0 0 0.05 0 0 0
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Mitochondria 0.26 0 0 0 0 0.1 0.11 – 0.2 0.15 0.44 0.26 0 0.07 0 0.12 0.08 – 0 0 0 0 0 0
Nucleus 0.81 0.83 0.85 0.84 0.87 0.84 0.81 – 0.78 0.78 0.7 0.78 0.85 0.69 0.73 0.38 0.48 – 0.59 0.64 0.58 0.6 0.63 0.59
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Nucleolus 0.05 0 0 0 0 0 0 – 0.07 0.08 0.16 0.08 0 0 0 0 0 – 0.15 0.13 0.07 0.1 0.13 0.12
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
SpindlePole 0 0 0.06 0 0.05 0 0 – 0 0 0 0 0 0.07 0 0.05 0 – 0.07 0 0.05 0.06 0.06 0.06
Vac/Vac Membrane 0 0.13 0.06 0 0 0 0 – 0 0 0 0 0 0 0 0.28 0.22 – 0.11 0.09 0.15 0.12 0.08 0.1
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 – 0 0 2 0 0 0
Bud 0 0 0 0 0 1 0 – 0 1 1 0 0 0 0 0 0 – 2 3 4 0 1 1
Bud Neck 0 0 1 1 2 0 1 – 0 3 1 1 0 0 0 0 0 – 0 1 0 0 0 1
Bud Site 0 0 0 0 0 2 1 – 3 4 13 8 0 0 0 0 0 – – – – – – –
Cell Periphery 0 0 1 0 0 0 0 – 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Cytoplasm 6 12 13 22 18 22 21 – 20 17 16 13 4 7 19 5 13 – 1 2 4 2 2 7
Endoplasmic Reticulum 0 0 1 0 0 0 0 – 0 0 0 0 0 0 0 4 5 – 0 1 1 0 0 0
Endosome 1 8 3 2 3 4 4 – 1 0 0 1 0 1 0 5 12 – 6 10 9 0 0 1
Golgi 1 1 0 0 0 0 0 – 0 0 0 0 0 1 0 0 1 – 0 1 0 0 0 0
Mitochondria 48 4 2 5 2 34 33 – 39 43 143 89 1 3 0 5 7 – 1 4 4 0 1 1
Nucleus 152 242 137 151 186 272 247 – 155 224 227 263 23 31 58 15 41 – 132 222 115 23 57 64
Nuclear Periphery 0 0 0 0 0 1 2 – 0 2 3 1 0 0 0 0 0 – 1 1 0 0 0 0
Nucleolus 9 2 2 2 1 9 12 – 13 24 51 26 0 2 1 0 0 – 32 46 14 4 12 13
Peroxisomes 0 1 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
SpindlePole 4 5 10 6 10 3 5 – 6 9 0 7 0 3 1 2 3 – 15 15 9 2 5 6
Vac/Vac Membrane 6 37 10 1 5 8 7 – 2 4 4 6 0 2 1 11 19 – 24 31 29 4 7 10
Unique Cell Count 187 290 162 180 214 324 306 200 287 325 339 27 45 79 40 85 225 348 200 40 91 109
Labelled Cell Count 227 312 180 190 227 356 333 239 331 459 415 28 50 80 47 101 225 348 200 40 91 109


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.3 5.8 6.0 6.1 6.7 5.5 5.9 – 4.9 5.4 4.8 4.9 6.2 6.7 5.7 6.2 7.1 – – 6.0 6.9
Std Deviation (1e-4) 0.5 0.9 1.3 1.6 2.2 1.5 2.0 – 1.3 1.5 1.5 1.0 0.9 3.0 0.8 1.0 2.2 – – 1.4 2.3
Intensity Change (Log2) – – – 0.01 0.15 -0.13 -0.02 – -0.3 -0.15 -0.32 -0.31 0.03 0.14 -0.08 0.03 0.24 – – 0.0 0.19


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 – 0 0 0 0 0 0 0 0 0 –
Bud 0 0 0 0 – 0 0 0 0 0 0 0 0 0 –
Bud Neck 0 0 0 0 – 0 0 0 0 0 0 0 0 0 –
Bud Site 0 0 0 0 – 0 0 0 0 0 0 0 0 0 –
Cell Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 –
Cytoplasm 1.3 0.1 -0.5 -0.5 – 0.6 -0.9 -1.4 -2.0 0 0 3.4 0 1.8 –
Endoplasmic Reticulum 0 0 0 0 – 0 0 0 0 0 0 0 0 0 –
Endosome 0 0 0 0 – 0 0 0 0 0 0 0 0 3.8 –
Golgi 0 0 0 0 – 0 0 0 0 0 0 0 0 0 –
Mitochondria 0 0 3.7 3.7 – 5.5 4.7 0 6.8 0 0 0 0 0 –
Nucleus -0.2 0.6 -0.2 -1.0 – -1.7 -1.7 -3.5 -1.8 0.1 -2.4 -2.1 -6.2 -6.0 –
Nuclear Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 –
Nucleolus 0 0 0 0 – 2.5 3.1 0 2.9 0 0 0 0 0 –
Peroxisomes 0 0 0 0 – 0 0 0 0 0 0 0 0 0 –
SpindlePole -1.2 -0.6 0 -2.7 – -1.5 -1.5 0 -2.4 0 0 0 0 0 –
Vacuole -2.9 -1.9 -2.0 -2.1 – -2.7 -2.8 0 -2.6 0 0 0 0 3.8 –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -0.1185 -0.0337 -0.0769 -0.0488 -0.7056 -0.2374 0.8659 1.49 0.9212 0.6395 0.5355 0.7448 0.7364 0.9704 0.7311 0.839 0.8061 0.4513
Actin 0.024 0.0001 0.0038 0.0186 0.0006 0.0023 0.0093 0 0.0022 0.0009 0.0005 0.0001 0.0142 0 0.0001 0.0001 0.0005 0
Bud 0.0012 0 0.0004 0.0003 0.0003 0.0002 0.0005 0.0001 0.0003 0.0002 0.0016 0 0.0001 0 0 0.0001 0.0001 0
Bud Neck 0.0023 0.0004 0.0014 0.0003 0.002 0.0033 0.0045 0.0002 0.001 0.0009 0.0018 0.001 0.0016 0.0002 0.0002 0.0007 0.0008 0.0009
Bud Periphery 0.0038 0 0.0005 0.0004 0.0005 0.0005 0.0015 0 0.0002 0.0002 0.001 0 0.0003 0.0001 0 0.0001 0.0001 0
Bud Site 0.0069 0.0005 0.0093 0.0005 0.001 0.0006 0.005 0.0006 0.0006 0.0006 0.0018 0 0.0004 0.0001 0.0001 0.0001 0.0005 0
Cell Periphery 0.0019 0 0.0002 0 0.0001 0.0001 0.0005 0 0 0 0.0002 0 0.0001 0 0 0 0 0
Cytoplasm 0.0059 0.0014 0.0037 0.0003 0.0041 0.0006 0.0034 0.0031 0.0013 0.001 0.0187 0.0002 0.0016 0.0003 0.0006 0.0015 0.0005 0.0001
Cytoplasmic Foci 0.006 0.0003 0.0085 0.0053 0.0073 0.002 0.008 0.0009 0.0043 0.0105 0.0145 0.0001 0.0021 0 0.0001 0 0.0098 0
Eisosomes 0.0004 0 0.0001 0.0001 0 0.0001 0.0002 0 0 0 0.0001 0 0.0002 0 0 0 0 0
Endoplasmic Reticulum 0.0073 0.0002 0.0004 0.0001 0.002 0.0004 0.0009 0.0001 0.0001 0.0001 0.0007 0 0.0021 0.0001 0.0001 0 0.0001 0
Endosome 0.0307 0.0002 0.0086 0.0047 0.0458 0.0135 0.0077 0.0001 0.0041 0.0012 0.012 0 0.008 0.0002 0.0003 0.0001 0.0026 0
Golgi 0.0143 0 0.0052 0.0096 0.0098 0.006 0.0026 0 0.0049 0.0021 0.0016 0 0.0022 0 0 0 0.0012 0
Lipid Particles 0.0071 0 0.0253 0.0322 0.0225 0.0109 0.0063 0 0.0033 0.002 0.0019 0 0.0017 0 0.0001 0 0.0047 0
Mitochondria 0.0207 0.0001 0.0166 0.0097 0.0198 0.0171 0.0144 0.0001 0.0031 0.0003 0.0239 0.0001 0.0015 0.0002 0.0002 0.0002 0.0008 0
None 0.0168 0.0009 0.0013 0.0002 0.0015 0.002 0.0053 0.014 0.0056 0.0004 0.013 0.0027 0.002 0.0001 0.0003 0.0001 0.0003 0
Nuclear Periphery 0.035 0.0162 0.009 0.0037 0.0255 0.0041 0.0103 0.0032 0.0021 0.004 0.0091 0.0011 0.0117 0.0041 0.0118 0.0027 0.0034 0.0006
Nucleolus 0.0253 0.0428 0.0286 0.0136 0.0677 0.0439 0.0353 0.029 0.024 0.0299 0.0597 0.0538 0.0215 0.0229 0.0241 0.0183 0.0796 0.0453
Nucleus 0.7506 0.8908 0.8364 0.8917 0.729 0.8554 0.8428 0.9229 0.8857 0.8257 0.7104 0.9044 0.9081 0.9673 0.9496 0.9676 0.833 0.9466
Peroxisomes 0.0045 0 0.0051 0.0048 0.0058 0.0031 0.0042 0 0.002 0.0194 0.0025 0 0.0005 0 0 0 0.0127 0
Punctate Nuclear 0.0258 0.0455 0.0343 0.0036 0.0447 0.03 0.0332 0.0257 0.0545 0.1004 0.1195 0.0363 0.0187 0.0034 0.0122 0.0083 0.0489 0.0061
Vacuole 0.0068 0.0001 0.0012 0.0002 0.0073 0.0024 0.003 0.0001 0.0004 0.0003 0.002 0 0.0007 0.0004 0.0001 0.0001 0.0002 0.0001
Vacuole Periphery 0.0026 0.0002 0.0004 0.0002 0.0027 0.0014 0.0012 0 0.0001 0.0001 0.0037 0 0.0006 0.0004 0.0002 0.0001 0.0002 0

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 10.594 13.1128 6.8472 11.2749 13.0312 15.7464 14.3632 13.9199 7.1796 14.6728
Translational Efficiency 0.7737 0.7813 0.891 0.469 0.5636 0.5731 0.5856 0.4963 0.8989 0.6388

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
160 878 730 1213 1999 1893 146 963 2159 2771 876 2176

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 590.37 732.66 882.48 892.96 719.29 752.17 992.79 905.28 709.74 745.99 900.87 898.41
Standard Deviation 64.07 101.96 126.86 119.81 83.71 100.20 112.78 133.87 89.07 101.17 131.23 126.37
Intensity Change Log 2 — 0.311524 0.579944 0.596976 — 0.064485 0.464915 0.331791 — 0.181105 0.517906 0.457393

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000180 0.000708 0.001083 0.001444 0.000154 0.000344 0.001490 0.002737 0.000156 0.000459 0.001151 0.002017
Bud Neck 0.000409 0.004968 0.010673 0.015224 0.004079 0.008571 0.006408 0.018437 0.003807 0.007429 0.009962 0.016646
Bud Site 0.001292 0.001973 0.003483 0.027670 0.000650 0.002907 0.001566 0.021269 0.000698 0.002611 0.003163 0.024837
Cell Periphery 0.000102 0.000129 0.000107 0.000311 0.000072 0.000097 0.000115 0.000167 0.000074 0.000107 0.000108 0.000247
Cytoplasm 0.084915 0.052963 0.018072 0.091461 0.042341 0.039265 0.010920 0.052481 0.045497 0.043605 0.016880 0.074210
Cytoplasmic Foci 0.030515 0.010971 0.000495 0.006857 0.006696 0.009813 0.000551 0.007268 0.008461 0.010180 0.000504 0.007039
Eisosomes 0.000060 0.000045 0.000039 0.000047 0.000025 0.000035 0.000081 0.000041 0.000028 0.000038 0.000046 0.000044
Endoplasmic Reticulum 0.000608 0.003851 0.007830 0.007338 0.001570 0.002764 0.013638 0.006498 0.001498 0.003108 0.008798 0.006966
Endosome 0.000562 0.001909 0.000647 0.005211 0.001077 0.003189 0.001119 0.012914 0.001039 0.002783 0.000726 0.008620
Golgi 0.000311 0.000838 0.000128 0.002700 0.000476 0.001644 0.000139 0.005915 0.000464 0.001389 0.000130 0.004123
Lipid Particles 0.003316 0.003451 0.000656 0.000893 0.002319 0.003800 0.001245 0.000929 0.002393 0.003689 0.000754 0.000909
Mitochondria 0.000888 0.006687 0.001150 0.002634 0.003049 0.009478 0.000999 0.005529 0.002889 0.008594 0.001125 0.003915
Mitotic Spindle 0.000027 0.003870 0.002428 0.029856 0.002989 0.011512 0.000804 0.047417 0.002769 0.009091 0.002157 0.037628
None 0.048236 0.019864 0.006961 0.013680 0.010493 0.012108 0.006556 0.012450 0.013290 0.014566 0.006893 0.013136
Nuclear Periphery 0.000262 0.002173 0.001099 0.002153 0.002155 0.002905 0.001954 0.003397 0.002014 0.002673 0.001241 0.002704
Nuclear Periphery Foci 0.000428 0.003075 0.000942 0.000952 0.001077 0.001517 0.007831 0.001457 0.001029 0.002011 0.002090 0.001176
Nucleolus 0.024329 0.035305 0.010638 0.004712 0.024078 0.034301 0.015510 0.005517 0.024097 0.034619 0.011450 0.005068
Nucleus 0.799395* 0.841197* 0.928016* 0.762433* 0.894430* 0.847258* 0.914604* 0.751036* 0.887387* 0.845337* 0.925781* 0.757389*
Peroxisomes 0.002531 0.002941 0.000305 0.000522 0.001149 0.002979 0.000417 0.001188 0.001251 0.002967 0.000324 0.000817
Vacuole 0.001529 0.002029 0.005062 0.022641 0.000943 0.004335 0.013834 0.034668 0.000986 0.003605 0.006524 0.027964
Vacuole Periphery 0.000105 0.001050 0.000186 0.001260 0.000179 0.001177 0.000220 0.008684 0.000174 0.001137 0.000191 0.004546

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -5.39 -9.51 -10.04 -6.00 -1.28 -4.83 -8.33 -5.10 -4.47 0.46 -7.95 -14.04 -9.71 -7.57 -1.02
Bud Neck -7.10 -11.09 -17.02 -11.70 -3.04 -5.55 -2.69 -12.53 -9.05 -6.12 -5.83 -8.74 -18.19 -13.46 -5.38
Bud Site -0.61 -2.11 -6.37 -6.57 -5.45 -4.36 -4.80 -6.52 -5.14 -5.02 -4.67 -4.65 -9.60 -8.16 -6.80
Cell Periphery -0.99 0.01 -4.94 -3.73 -5.50 -1.87 -4.77 -8.74 -3.85 -0.06 -2.99 -3.87 -9.90 -5.84 -5.37
Cytoplasm 2.56 5.69 2.11 -1.13 -10.93 0.87 8.15 -0.02 -0.73 -6.77 0.62 11.23 -1.77 -2.47 -12.63
Cytoplasmic Foci 3.48 5.14 4.78 4.88 -3.29 -2.33 9.16 5.55 6.67 -2.64 -0.95 10.53 7.14 8.07 -4.33
Eisosomes 2.08 2.05 -0.12 -4.20 -4.05 -4.76 -7.54 -11.39 -6.81 4.30 -5.60 -8.16 -14.75 -8.90 -1.24
Endoplasmic Reticulum -12.48 -17.28 -22.15 -14.30 -3.35 -6.04 -10.44 -17.80 -14.80 4.55 -10.19 -18.51 -27.12 -21.41 -0.54
Endosome -2.11 -0.60 -3.64 -1.29 -3.58 -3.14 -1.60 -3.54 -1.18 -2.85 -3.24 0.07 -4.25 -1.23 -5.05
Golgi -1.72 1.68 -1.26 -0.27 -1.97 -2.92 2.26 -1.92 0.11 -2.48 -3.17 2.67 -2.16 0.35 -3.18
Lipid Particles 0.25 3.42 3.12 4.72 -1.72 -2.60 2.21 4.85 5.81 2.72 -2.75 5.14 5.13 7.13 -0.44
Mitochondria -3.32 -2.01 -5.63 2.08 -5.39 -5.37 4.04 -1.60 4.72 -7.25 -5.56 3.82 -1.03 5.40 -8.84
Mitotic Spindle -3.50 -4.42 -8.79 -6.76 -7.07 -4.28 0.94 -8.40 -6.04 -8.76 -4.16 -0.68 -10.98 -8.16 -10.75
None 2.92 4.48 4.12 5.27 -2.43 -1.28 4.10 0.95 2.01 -2.94 -0.99 6.11 3.38 4.71 -3.46
Nuclear Periphery -4.69 -12.68 -18.84 -3.59 -9.88 -1.99 -1.23 -5.99 -4.03 -1.11 -2.02 0.23 -7.77 -5.17 -8.11
Nuclear Periphery Foci -3.53 -2.12 -2.72 2.56 0.04 -1.02 -1.42 -0.66 0.38 1.36 -2.97 -1.73 -1.34 2.22 1.26
Nucleolus -1.86 2.63 4.59 13.05 5.90 -4.42 3.35 11.81 14.65 1.94 -5.25 6.15 13.70 19.64 6.07
Nucleus -2.30 -6.20 -0.38 4.04 14.32 7.01 0.07 10.50 4.94 7.04 7.00 -4.95 13.10 6.70 17.12
Peroxisomes 0.06 2.18 2.15 4.84 -0.46 -4.33 3.99 3.84 6.58 0.04 -4.59 5.06 4.72 8.22 -0.70
Vacuole -1.31 -6.32 -13.24 -13.10 -10.48 -4.73 -6.28 -11.25 -10.21 -2.87 -5.29 -9.41 -17.52 -16.12 -11.80
Vacuole Periphery -2.53 -4.24 -3.15 1.02 -2.54 -4.28 -2.17 -2.88 -1.54 -2.78 -4.87 -2.57 -3.49 -0.72 -3.35
Standard name
Human Ortholog
Description MADS-box transcription factor; component of the protein kinase C-mediated MAP kinase pathway involved in the maintenance of cell integrity; phosphorylated and activated by the MAP-kinase Slt2p; RLM1 has a paralog, SMP1, that arose from the whole genome duplication
Localization
Cell Percentages nucleus (97%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-3

Rlm1

Rlm1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Rlm1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available