Standard name
Human Ortholog
Description Phosphoinositide binding protein; required for vesicle formation in the cytoplasm-to-vacuole targeting (CVT) pathway, autophagy, micronucleophagy and mitophagy; binds to several phosphoinositides including: PtdIns(3,5)P2, PI3P and PI4P; involved in PI3P-dependent recruitment and organization of both the Atg12-Atg5-Atg16 complex and Atg8p at the pre-autophagosomal structure; necessary for oxidant-induced cell death; WD-40 repeat containing PROPPIN family member
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0.06 0 0 0.06 0 0.07 0.08 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0.09 0.09 0.15 0.14 0.19 0.21 0.2 0.14 0.22 0.21 0 0 0.05 0.22 0.17 0.1 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0.24 0.51 0.46 0.51 0.45 0.38 0.29 0.26 0.16 0.11 0.08 0.08 0.75 0.82 0.8 0.58 0.57 0.62 0.74 0.76 0.75 0.83 0.82 0.78
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.63 0.11 0.32 0.28 0.32 0.44 0.59 0.53 0.59 0.75 0.67 0.68 0 0 0 0.1 0.08 0.06 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.2 0.07 0.24 0.15 0 0 0 0 0 0.05 0 0 0.18 0.22 0.13 0 0.08 0.08 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0.07 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0.1 0.05 0.18 0.18 0.07 0.13 0.06 0.08 0.05 0.09 0.06 0.07 0.15 0.19 0.13 0.13 0.17 0.25 0 0 0.06 0.07 0 0
Vac/Vac Membrane 0.38 0.55 0.15 0.3 0.19 0.12 0.11 0.16 0.1 0.08 0.08 0.11 0.45 0.38 0.4 0.43 0.38 0.39 0.14 0.11 0.06 0 0.06 0.1
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 1 3 0 0 0 0 1 0 1 1 0 1 0 0 0 2 1 1 1 0 0 1 0
Bud 4 1 3 0 4 10 3 8 11 6 10 12 0 0 0 1 0 1 0 1 0 0 2 4
Bud Neck 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 1 0 2 1 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 4 8 10 8 18 21 31 47 34 25 32 31 16 9 21 22 24 11 0 2 2 0 3 3
Endoplasmic Reticulum 0 5 2 0 1 0 1 3 7 8 5 1 1 0 1 2 6 5 0 0 1 0 0 1
Endosome 63 120 52 45 55 59 48 59 28 19 11 12 277 249 339 59 82 70 201 199 95 168 234 247
Golgi 0 1 0 0 3 0 1 0 0 0 0 0 0 0 0 1 4 2 3 3 0 0 2 3
Mitochondria 163 25 36 25 39 68 98 120 103 133 97 103 16 7 8 10 11 7 10 7 2 2 5 9
Nucleus 2 2 1 1 0 1 0 1 1 0 2 1 3 2 1 7 4 0 0 0 0 0 0 0
Nuclear Periphery 1 7 3 1 0 1 1 0 0 1 0 0 5 6 3 3 1 2 2 3 2 2 2 1
Nucleolus 53 17 27 13 5 5 6 4 2 8 2 6 66 67 55 4 12 9 0 0 0 0 1 0
Peroxisomes 0 1 1 2 0 11 6 7 5 4 1 4 11 6 9 0 1 0 2 1 1 2 1 2
SpindlePole 26 11 20 16 9 20 10 19 9 16 9 11 54 58 56 13 24 28 9 8 7 14 10 5
Vac/Vac Membrane 99 129 17 27 23 18 19 36 17 14 12 17 168 114 172 43 55 44 37 27 7 8 18 32
Unique Cell Count 259 234 113 89 121 154 167 228 174 177 144 151 371 302 426 101 143 113 272 263 127 204 285 317
Labelled Cell Count 415 328 175 138 157 214 225 305 217 236 182 201 619 518 665 165 226 180 272 263 127 204 285 317


Ambiguous, Endosome

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.0 6.2 5.3 5.6 5.6 4.9 4.5 5.0 4.8 4.5 4.8 4.7 6.4 6.4 6.5 7.1 7.9 7.9 5.3 5.5 6.2
Std Deviation (1e-4) 0.6 1.1 1.1 1.5 1.8 1.2 1.4 1.4 1.1 1.3 1.4 1.4 1.2 1.1 2.0 2.0 1.5 1.4 1.3 1.2 1.3
Intensity Change (Log2) – – – 0.08 0.08 -0.1 -0.23 -0.09 -0.14 -0.23 -0.14 -0.17 0.28 0.27 0.3 0.43 0.57 0.57 0.01 0.04 0.24


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 1.4 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 1.4 1.2 2.3 2.7 2.5 1.3 2.9 2.6 -1.9 -2.5 -1.6 2.6 1.9 0.2
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0.6 -0.1 -1.3 0 -3.7 0 0 0 0 5.7 7.4 7.1 1.8 1.8 2.4
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria -0.6 0.1 2.0 0 3.6 0 0 0 0 -8.3 -8.8 -10.3 -3.9 -5.0 -4.9
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus -1.6 -4.4 -5.1 0 -6.7 0 0 0 0 -1.4 -0.4 -2.9 -4.1 -3.4 -3.3
Peroxisomes 0 0 2.4 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0.1 -2.4 -1.1 0 -2.6 0 0 0 0 -0.8 0.3 -1.2 -1.0 -0.2 1.3
Vacuole 2.6 0.8 -0.8 -0.9 0.2 -1.4 -1.9 -1.7 -0.9 5.8 4.4 5.0 4.5 4.1 4.0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.8174 3.2673 2.8224 2.311 2.3838 2.8223 3.3476 3.9143 3.957 3.3878 3.1371 3.9398 4.528 5.6473 5.2962 5.2875 4.6449 5.2825
Actin 0.0275 0 0.0248 0.0575 0.0451 0.0018 0.0424 0.0005 0.0433 0.0269 0 0.004 0.0004 0 0.0003 0 0 0.0011
Bud 0.0002 0.0001 0.0003 0.0004 0.0004 0.0002 0.0003 0.0004 0.0006 0.0003 0 0.0001 0.0001 0.0001 0.0001 0 0 0.0001
Bud Neck 0.0006 0.0004 0.0011 0.0009 0.0003 0.0007 0.0008 0.0004 0.0008 0.0004 0.0001 0.0003 0.0004 0.0007 0.0005 0.0001 0.0001 0.0006
Bud Periphery 0.0002 0.0001 0.0005 0.0006 0.0008 0.0004 0.0004 0.0002 0.0008 0.0004 0 0.0001 0.0001 0.0001 0.0002 0 0 0.0001
Bud Site 0.0048 0.0007 0.0021 0.0022 0.0009 0.0005 0.0023 0.0021 0.0244 0.0009 0.0001 0.0002 0.0003 0.0004 0.0007 0.0001 0.0001 0.0003
Cell Periphery 0.0001 0.0001 0.0001 0.0002 0.0002 0.0002 0.0002 0.0002 0.0006 0.0004 0 0.0001 0.0001 0.0001 0.0002 0 0 0.0001
Cytoplasm 0.0213 0.0071 0.0017 0.0011 0.0012 0.0043 0.0068 0.0028 0.0064 0.0016 0.0018 0.0009 0.0105 0.0017 0.0034 0.0013 0.001 0.0015
Cytoplasmic Foci 0.1295 0.1132 0.1297 0.0955 0.0954 0.0927 0.1104 0.1425 0.1596 0.132 0.0753 0.0929 0.1269 0.1035 0.1403 0.1168 0.1003 0.0881
Eisosomes 0.0001 0 0.0002 0.0005 0.0005 0 0.0003 0 0.0004 0.0002 0 0 0 0 0 0 0 0.0001
Endoplasmic Reticulum 0.0012 0.0005 0.0005 0.0014 0.0016 0.0011 0.0007 0.0004 0.0034 0.0018 0.0006 0.0004 0.0014 0.0013 0.0006 0.0001 0.0003 0.0005
Endosome 0.4255 0.512 0.5289 0.615 0.3948 0.4421 0.4704 0.4892 0.3503 0.5108 0.7177 0.5198 0.4944 0.5182 0.5125 0.6985 0.6762 0.4946
Golgi 0.0408 0.0418 0.0693 0.0706 0.0524 0.0549 0.0583 0.0508 0.0608 0.1267 0.0772 0.0629 0.0403 0.0429 0.0706 0.0688 0.0753 0.0623
Lipid Particles 0.2369 0.1973 0.1441 0.0997 0.2898 0.2911 0.1976 0.2165 0.1986 0.0809 0.052 0.2116 0.229 0.2172 0.1679 0.0544 0.1097 0.2594
Mitochondria 0.0018 0.0014 0.0041 0.0045 0.0152 0.0075 0.016 0.0026 0.0288 0.0213 0.0016 0.0023 0.002 0.0019 0.0107 0.0005 0.0009 0.0025
None 0.0017 0.0003 0.0118 0.0071 0.0039 0.0003 0.0236 0.0005 0.0017 0.0003 0.0001 0.0002 0.0043 0.0005 0.0009 0.0001 0.0001 0.0077
Nuclear Periphery 0.0027 0.0023 0.0013 0.0028 0.0276 0.0032 0.001 0.0012 0.0015 0.0009 0.0009 0.0014 0.0031 0.0019 0.001 0.0002 0.0005 0.0025
Nucleolus 0.0014 0.0014 0.0026 0.0007 0.0014 0.0025 0.0006 0.0019 0.0009 0.0003 0.0001 0.0008 0.0015 0.0018 0.0011 0.0001 0.0001 0.0012
Nucleus 0.0027 0.0006 0.0007 0.0005 0.0014 0.0037 0.0021 0.0003 0.0005 0.0003 0.0001 0.0007 0.0019 0.0004 0.0005 0.0001 0.0001 0.0007
Peroxisomes 0.0324 0.0254 0.0292 0.0252 0.0372 0.0217 0.0487 0.0297 0.0681 0.0283 0.0128 0.0399 0.0349 0.0219 0.0283 0.0272 0.0146 0.0366
Punctate Nuclear 0.047 0.0048 0.0033 0.0027 0.0032 0.0057 0.0051 0.0024 0.0024 0.0006 0.0002 0.0008 0.009 0.0009 0.0011 0.0004 0.0013 0.003
Vacuole 0.0154 0.0709 0.0352 0.0072 0.02 0.0499 0.0085 0.0366 0.0343 0.0489 0.0288 0.0453 0.0292 0.0549 0.0423 0.0258 0.0131 0.0281
Vacuole Periphery 0.0062 0.0195 0.0084 0.0037 0.0068 0.0154 0.0035 0.0185 0.0117 0.0157 0.0304 0.0156 0.0102 0.0297 0.0168 0.0053 0.0061 0.0089

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 9.9906 4.6309 11.2907 9.8067 9.595 8.136 8.0268 10.6093 11.1938 13.1804
Translational Efficiency 0.862 1.706 0.6568 1.1449 1.0178 1.4559 1.4865 1.029 1.1751 0.7648

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Phosphoinositide binding protein; required for vesicle formation in the cytoplasm-to-vacuole targeting (CVT) pathway, autophagy, micronucleophagy and mitophagy; binds to several phosphoinositides including: PtdIns(3,5)P2, PI3P and PI4P; involved in PI3P-dependent recruitment and organization of both the Atg12-Atg5-Atg16 complex and Atg8p at the pre-autophagosomal structure; necessary for oxidant-induced cell death; WD-40 repeat containing PROPPIN family member
Localization
Cell Percentages endosome (78%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Atg21

Atg21


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Atg21-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available