Standard name
Human Ortholog
Description Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to mitochondria; YPL107W is not an essential gene
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 – – – – – –
Cell Periphery 0.05 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 – – 0.08 0 0 0 0 0 0
Cytoplasm 0 0.05 0 0 0.08 0 0 0 0 0 0 0 0 0 0 – – 0.2 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.05 – – 0 0 0 0 0 0 0
Endosome 0.05 0 0.06 0 0.1 0.05 0 0 0 0 0 0 0.05 0.09 0.05 – – 0 0.05 0 0.05 0.05 0.05 0
Golgi 0 0 0.13 0.19 0.22 0.06 0 0 0 0 0 0 0.14 0.21 0.22 – – 0.32 0.05 0.07 0 0 0.06 0.05
Mitochondria 0.92 0.92 0.71 0.83 0.67 0.8 0.69 0.81 0.9 0.87 0.89 0.86 0.84 0.75 0.71 – – 0 0.79 0.76 0.77 0.72 0.71 0.74
Nucleus 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0 0
Nuclear Periphery 0.05 0 0 0 0 0.07 0.08 0.06 0.09 0.11 0.05 0.07 0 0 0 – – 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0.05 0.08 0.06 0.14 0.1 0.11 0.08 0 0 0 – – 0 0 0 0 0 0 0
Peroxisomes 0 0 0.12 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0.09 0 0.05 0
SpindlePole 0 0 0.08 0 0 0 0.07 0 0 0 0 0 0 0 0 – – 0.08 0 0 0 0 0 0
Vac/Vac Membrane 0.24 0.11 0.08 0.08 0.14 0.16 0.13 0.14 0.07 0 0.06 0.06 0.14 0.1 0.13 – – 0.2 0 0.07 0 0.05 0.05 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 2 1 8 4 0 0 0 2 3 1 0 1 7 9 0 – – 1 0 0 0 0 1 2
Bud 0 0 2 4 2 5 4 4 3 0 4 5 5 4 3 – – 1 1 0 0 1 0 1
Bud Neck 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 1 1
Bud Site 0 0 0 0 0 2 4 1 0 0 1 2 0 0 0 – – 0 – – – – – –
Cell Periphery 10 5 5 5 3 1 2 3 0 0 3 3 4 8 2 – – 2 0 0 0 0 0 0
Cytoplasm 1 7 3 3 6 6 9 8 2 3 4 2 2 3 8 – – 5 0 2 0 3 2 1
Endoplasmic Reticulum 1 1 1 2 1 1 3 1 1 2 0 2 4 10 15 – – 0 0 0 0 1 1 1
Endosome 10 0 9 3 8 10 8 9 0 0 2 5 8 19 13 – – 1 10 4 9 10 15 12
Golgi 3 1 20 21 17 11 3 0 0 1 2 2 23 43 62 – – 8 8 10 7 9 17 16
Mitochondria 175 133 109 92 52 152 147 177 111 138 246 241 133 153 197 – – 0 151 110 137 154 213 234
Nucleus 1 0 0 0 2 0 12 8 3 3 3 4 0 0 3 – – 0 0 0 0 3 3 3
Nuclear Periphery 9 6 3 1 2 14 18 14 11 18 15 21 3 3 9 – – 1 1 1 0 2 3 4
Nucleolus 6 0 3 1 1 9 17 12 17 16 29 23 3 0 4 – – 1 0 0 0 2 0 3
Peroxisomes 0 0 18 0 1 3 1 3 0 0 1 4 6 4 6 – – 0 4 1 15 8 14 14
SpindlePole 0 0 12 2 1 7 14 4 5 6 6 8 7 9 5 – – 2 2 0 1 1 3 1
Vac/Vac Membrane 45 16 12 9 11 30 28 31 9 7 16 18 23 20 37 – – 5 7 10 2 10 14 10
Unique Cell Count 191 144 154 111 78 191 213 218 123 158 275 280 159 205 279 25 192 145 179 213 300 315
Labelled Cell Count 263 170 206 147 107 251 270 277 165 195 332 341 228 285 364 27 192 145 179 213 300 315


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.4 5.1 7.1 6.8 7.7 6.7 6.5 6.6 5.6 6.0 5.8 6.4 6.7 6.7 6.5 – – 6.8 7.3 7.7 7.9
Std Deviation (1e-4) 1.3 1.0 1.4 1.3 1.7 1.3 1.3 1.1 1.1 1.5 1.2 1.3 1.1 1.0 1.1 – – 1.4 1.5 1.4 1.8
Intensity Change (Log2) – – – -0.06 0.12 -0.07 -0.13 -0.1 -0.34 -0.23 -0.29 -0.15 -0.08 -0.07 -0.12 – – -0.05 0.05 0.12 0.17


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches -0.6 0 0 0 0 0 0 0 0 -0.3 -0.4 0 – – 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 – – 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 – – 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 – – 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0.3 0 – – 0
Cytoplasm 0 0 0 1.2 0 0 0 0 0 0 0 0 – – 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 2.5 – – 0
Endosome -1.2 1.2 -0.2 -0.9 -0.8 0 0 0 0 -0.3 1.2 -0.5 – – 0
Golgi 1.3 1.7 -2.3 -4.5 -5.5 -4.1 -4.4 -5.5 -5.6 0.4 2.0 2.3 – – 0
Mitochondria 2.3 -0.6 1.9 -0.4 2.3 4.0 3.6 4.9 3.9 2.7 0.8 0 – – -6.7
Nucleus 0 0 0 3.0 0 0 0 0 0 0 0 0 – – 0
Nuclear Periphery 0 0 2.3 2.6 2.0 2.6 3.3 1.7 2.4 0 0 0 – – 0
Nucleolus 0 0 1.4 2.5 1.7 3.8 3.0 3.3 2.6 0 0 0 – – 0
Peroxisomes -3.7 -2.7 -3.9 -4.8 -4.2 -3.9 -4.4 -5.5 -4.7 -2.6 -3.8 -4.2 – – 0
SpindlePole -2.2 0 -1.7 -0.4 -2.8 -1.3 -1.5 -2.8 -2.3 -1.3 -1.4 -3.1 – – 0
Vacuole 0.1 1.5 2.2 1.6 1.9 -0.1 -1.2 -0.8 -0.5 1.9 0.6 1.7 – – 0