Standard name
Human Ortholog
Description Subunit of TFIID, TFIIF, INO80, SWI/SNF, and NuA3 complexes; involved in RNA polymerase II transcription initiation and in chromatin modification; contains a YEATS domain
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches – – 0 – 0 0 0 0 0 0 – – 0 0 0 – – – – 0 0 0 0 0
Bud – – 0 – 0 0 0 0 0 0 – – 0 0 0 – – – – 0 0 0 0 0
Bud Neck – – 0 – 0 0 0.05 0 0 0.1 – – 0 0 0 – – – – 0 0 0 0 0
Bud Site – – 0 – 0 0 0 0 0 0.05 – – 0 0 0 – – – – – – – – –
Cell Periphery – – 0 – 0 0 0 0 0 0 – – 0 0 0 – – – – 0 0 0 0 0
Cytoplasm – – 0 – 0.07 0.19 0.1 0.28 0.13 0.1 – – 0 0.11 0 – – – – 0 0.05 0 0 0
Endoplasmic Reticulum – – 0 – 0 0 0 0 0 0 – – 0 0 0 – – – – 0 0 0 0 0
Endosome – – 0 – 0 0 0 0 0 0 – – 0 0 0 – – – – 0 0 0 0 0
Golgi – – 0 – 0 0 0 0 0 0 – – 0 0 0 – – – – 0 0 0 0 0
Mitochondria – – 0 – 0 0 0.05 0 0.08 0.05 – – 0 0 0 – – – – 0.05 0 0 0 0
Nucleus – – 0.72 – 0.63 0.53 0.55 0.44 0.38 0.4 – – 0.54 0.41 0.62 – – – – 0.84 0.56 0.51 0.49 0.45
Nuclear Periphery – – 0 – 0 0 0 0 0 0 – – 0 0 0 – – – – 0 0 0 0 0
Nucleolus – – 0.64 – 0.43 0.28 0.5 0.21 0.6 0.75 – – 0.77 0.59 0.65 – – – – 0.07 0.28 0.2 0.13 0.33
Peroxisomes – – 0 – 0 0 0 0 0 0 – – 0 0 0 – – – – 0 0 0 0 0
SpindlePole – – 0 – 0 0 0 0 0 0 – – 0 0.07 0 – – – – 0 0 0.14 0.12 0.07
Vac/Vac Membrane – – 0 – 0 0.05 0 0.11 0 0 – – 0 0 0 – – – – 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches – – 0 – 0 0 0 0 0 0 – – 0 0 0 – – – – 0 0 0 1 0
Bud – – 0 – 0 1 1 0 0 0 – – 0 0 0 – – – – 0 0 0 2 2
Bud Neck – – 0 – 1 0 5 1 0 2 – – 0 0 0 – – – – 0 0 0 0 0
Bud Site – – 0 – 1 0 3 0 1 1 – – 0 0 1 – – – – – – – – –
Cell Periphery – – 0 – 0 0 0 0 0 0 – – 0 0 0 – – – – 0 0 0 0 0
Cytoplasm – – 1 – 3 11 11 33 7 2 – – 0 5 4 – – – – 0 3 0 3 2
Endoplasmic Reticulum – – 0 – 0 0 0 1 1 0 – – 0 0 0 – – – – 0 0 1 2 0
Endosome – – 0 – 0 0 1 0 0 0 – – 0 0 1 – – – – 0 0 3 2 2
Golgi – – 0 – 0 0 0 3 0 0 – – 0 0 0 – – – – 0 0 0 0 0
Mitochondria – – 0 – 0 2 5 1 4 1 – – 2 2 0 – – – – 0 0 0 2 3
Nucleus – – 26 – 29 30 61 52 20 8 – – 26 19 63 – – – – 17 36 40 59 64
Nuclear Periphery – – 0 – 0 0 0 0 0 0 – – 0 0 0 – – – – 0 0 0 2 0
Nucleolus – – 23 – 20 16 55 25 32 15 – – 37 27 66 – – – – 1 18 15 16 46
Peroxisomes – – 0 – 0 0 2 1 0 0 – – 0 0 0 – – – – 0 0 0 1 0
SpindlePole – – 0 – 0 1 0 3 0 0 – – 1 3 1 – – – – 0 2 11 14 9
Vac/Vac Membrane – – 0 – 0 3 1 13 1 0 – – 0 0 1 – – – – 0 1 0 5 5
Unique Cell Count 36 46 57 111 119 53 20 48 46 102 21 66 79 122 142
Labelled Cell Count 50 54 64 145 133 66 29 66 56 137 21 66 79 122 142


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) – – 10.3 – 11.0 11.4 9.0 12.9 8.7 7.7 – – 9.3 8.7 9.6 – – – 9.8 11.0 10.7
Std Deviation (1e-4) – – 2.9 – 3.3 4.4 3.0 4.1 3.2 2.1 – – 2.3 2.9 3.4 – – – 3.4 4.7 3.8
Intensity Change (Log2) – – – – 0.1 0.16 -0.19 0.33 -0.23 -0.41 – – -0.15 -0.23 -0.09 – – – -0.06 0.1 0.06


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches – 0 0 0 0 0 0 – – 0 0 0 – – –
Bud – 0 0 0 0 0 0 – – 0 0 0 – – –
Bud Neck – 0 0 0 0 0 0 – – 0 0 0 – – –
Bud Site – 0 0 0 0 0 0 – – 0 0 0 – – –
Cell Periphery – 0 0 0 0 0 0 – – 0 0 0 – – –
Cytoplasm – 0 0 0 3.2 0 0 – – 0 0 0 – – –
Endoplasmic Reticulum – 0 0 0 0 0 0 – – 0 0 0 – – –
Endosome – 0 0 0 0 0 0 – – 0 0 0 – – –
Golgi – 0 0 0 0 0 0 – – 0 0 0 – – –
Mitochondria – 0 0 0 0 0 0 – – 0 0 0 – – –
Nucleus – -0.9 -1.9 -1.8 -3.0 -3.2 -2.4 – – -1.7 -2.8 -1.1 – – –
Nuclear Periphery – 0 0 0 0 0 0 – – 0 0 0 – – –
Nucleolus – -1.8 -3.4 -1.5 -4.9 -0.3 0.9 – – 1.3 -0.5 0.1 – – –
Peroxisomes – 0 0 0 0 0 0 – – 0 0 0 – – –
SpindlePole – 0 0 0 0 0 0 – – 0 0 0 – – –
Vacuole – 0 0 0 0 0 0 – – 0 0 0 – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration – – – – – – – – – – – – – – – – – –
Actin 0.0019 0 0 0.0026 0.0006 0.002 – – – – – – 0.0174 0.0015 0 0 0.0064 0.0012
Bud 0.0006 0 0 0.0001 0.0001 0.0001 – – – – – – 0.0004 0.0003 0 0 0.0002 0.0001
Bud Neck 0.0136 0 0 0.0002 0.0005 0.0007 – – – – – – 0.0004 0.0002 0 0.0001 0.0002 0.0004
Bud Periphery 0.0007 0 0 0.0001 0.0002 0.0001 – – – – – – 0.0006 0.0004 0 0 0.0003 0.0002
Bud Site 0.0277 0 0 0.0006 0.0002 0.0001 – – – – – – 0.0011 0.0009 0.0002 0 0.0002 0.0001
Cell Periphery 0.0006 0 0 0 0 0 – – – – – – 0.0001 0.0002 0 0 0.0001 0
Cytoplasm 0.0017 0 0 0.0066 0.0009 0.0027 – – – – – – 0.0031 0.0026 0.0001 0 0.0005 0.0002
Cytoplasmic Foci 0.0092 0 0 0.0009 0.0001 0.0019 – – – – – – 0.0197 0.0031 0.0005 0 0.0066 0.0001
Eisosomes 0.0002 0 0 0 0 0.0001 – – – – – – 0.0001 0 0 0 0 0
Endoplasmic Reticulum 0.0002 0 0 0.0024 0.0001 0.0003 – – – – – – 0.001 0.0021 0 0 0.0003 0.0004
Endosome 0.0034 0 0 0.007 0.0006 0.0041 – – – – – – 0.0082 0.0109 0.0001 0 0.0056 0.0012
Golgi 0.0016 0 0 0.0004 0.0001 0.0026 – – – – – – 0.0048 0.013 0 0 0.0042 0.0003
Lipid Particles 0.0052 0 0 0.0004 0 0.023 – – – – – – 0.0214 0.0012 0.0001 0 0.0253 0.0001
Mitochondria 0.0036 0.0001 0 0.0033 0.0012 0.0008 – – – – – – 0.0031 0.0067 0.0002 0.0002 0.0038 0.004
None 0.0033 0 0 0.0023 0.0007 0.0569 – – – – – – 0.0023 0.0005 0.0001 0 0.0007 0.0001
Nuclear Periphery 0.0021 0.0003 0.0001 0.0705 0.0177 0.0312 – – – – – – 0.0235 0.005 0.0003 0.0001 0.0125 0.0108
Nucleolus 0.1025 0.0281 0.0198 0.0202 0.2818 0.0437 – – – – – – 0.0905 0.081 0.0383 0.053 0.2518 0.2182
Nucleus 0.7827 0.9714 0.9799 0.8616 0.6566 0.8043 – – – – – – 0.7323 0.8607 0.9407 0.9463 0.6473 0.7493
Peroxisomes 0.0039 0 0 0.0001 0.0001 0.0019 – – – – – – 0.007 0.0003 0 0 0.0161 0
Punctate Nuclear 0.0331 0.0001 0.0001 0.0181 0.038 0.0225 – – – – – – 0.0589 0.0082 0.0194 0.0001 0.0171 0.0117
Vacuole 0.0014 0 0 0.001 0.0002 0.0002 – – – – – – 0.0028 0.0007 0 0.0001 0.0003 0.0003
Vacuole Periphery 0.0006 0 0 0.0014 0.0003 0.0008 – – – – – – 0.0012 0.0006 0 0 0.0006 0.0012

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 65.3035 42.9443 51.7831 56.5789 72.9904 52.514 50.2054 45.7909 59.5172 59.9395
Translational Efficiency 2.4328 3.0898 2.1508 2.4248 2.0074 2.7666 2.28 2.0874 1.797 2.1074

Hit Data

Dataset Hit
Protein Concentration –
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1041 385 2022 1374 2450 1170 2962 1183 3491 1555 4984 2557

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 910.46 1333.42 1731.90 1597.62 1163.11 1370.54 1531.25 1645.92 1087.77 1361.35 1612.65 1619.97
Standard Deviation 194.38 305.35 351.76 341.06 269.69 289.82 289.20 387.28 275.08 294.17 331.08 363.97
Intensity Change Log 2 — 0.550464 0.927688 0.811257 — 0.236757 0.396722 0.500907 — 0.382957 0.654149 0.645452

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000716 0.000915 0.000763 0.001046 0.000605 0.001024 0.000581 0.001349 0.000638 0.000997 0.000655 0.001186
Bud Neck 0.005321 0.013329 0.015329 0.020490 0.009135 0.013917 0.013681 0.019741 0.007998 0.013771 0.014349 0.020144
Bud Site 0.000492 0.001054 0.001151 0.003214 0.000658 0.001597 0.000787 0.003353 0.000609 0.001463 0.000935 0.003278
Cell Periphery 0.000074 0.000028 0.000047 0.000187 0.000053 0.000059 0.000046 0.000110 0.000059 0.000051 0.000047 0.000151
Cytoplasm 0.000164 0.000107 0.000085 0.000736 0.000070 0.000218 0.000050 0.001044 0.000098 0.000191 0.000064 0.000878
Cytoplasmic Foci 0.000314 0.000414 0.000079 0.001588 0.000059 0.000375 0.000031 0.002318 0.000135 0.000385 0.000050 0.001926
Eisosomes 0.000057 0.000079 0.000102 0.000086 0.000076 0.000074 0.000085 0.000093 0.000070 0.000075 0.000092 0.000089
Endoplasmic Reticulum 0.001615 0.000766 0.000391 0.000660 0.000776 0.001110 0.000451 0.000715 0.001026 0.001025 0.000427 0.000685
Endosome 0.000093 0.000072 0.000020 0.002628 0.000053 0.000512 0.000013 0.003353 0.000065 0.000403 0.000016 0.002963
Golgi 0.000224 0.000152 0.000065 0.000966 0.000110 0.000238 0.000043 0.002351 0.000144 0.000216 0.000052 0.001607
Lipid Particles 0.000782 0.000398 0.000007 0.000350 0.000015 0.000238 0.000004 0.000607 0.000244 0.000278 0.000005 0.000469
Mitochondria 0.002089 0.002434 0.002253 0.004566 0.001443 0.002993 0.001519 0.005659 0.001636 0.002855 0.001816 0.005072
Mitotic Spindle 0.000498 0.007698 0.001157 0.010778 0.001172 0.017299 0.000473 0.013076 0.000971 0.014922 0.000750 0.011841
None 0.002997 0.000844 0.000672 0.001645 0.000861 0.000973 0.000573 0.001856 0.001498 0.000941 0.000613 0.001743
Nuclear Periphery 0.000084 0.000101 0.000131 0.000625 0.000164 0.000208 0.000084 0.000718 0.000140 0.000182 0.000103 0.000668
Nuclear Periphery Foci 0.000228 0.000089 0.000026 0.000267 0.000215 0.000269 0.000009 0.000270 0.000219 0.000225 0.000016 0.000268
Nucleolus 0.088654 0.125330 0.031250 0.038961 0.070319 0.090194 0.036218 0.044373 0.075787 0.098893 0.034203 0.041465
Nucleus 0.892912* 0.840648* 0.943694* 0.905513* 0.912320* 0.864705* 0.943736* 0.891563* 0.906533* 0.858749* 0.943719* 0.899059*
Peroxisomes 0.002342 0.004885 0.002321 0.003120 0.001298 0.003126 0.001246 0.003601 0.001609 0.003562 0.001682 0.003342
Vacuole 0.000163 0.000298 0.000236 0.001748 0.000212 0.000464 0.000175 0.002215 0.000198 0.000423 0.000200 0.001965
Vacuole Periphery 0.000182 0.000361 0.000223 0.000828 0.000384 0.000404 0.000197 0.001635 0.000324 0.000394 0.000207 0.001201

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -4.29 -5.27 -10.85 -3.41 -7.60 -6.28 -0.67 -3.54 -1.54 -3.46 -7.26 -3.84 -6.20 -2.34 -5.28
Bud Neck -10.53 -18.81 -13.69 -6.16 -4.04 -7.80 -12.23 -9.73 -5.34 -5.51 -11.49 -20.61 -15.73 -8.00 -7.11
Bud Site -6.86 -13.18 -7.17 -5.21 -4.57 -4.01 -2.36 -5.90 -3.52 -5.47 -5.00 -7.54 -9.02 -5.30 -7.37
Cell Periphery 1.72 0.91 -1.97 -3.40 -2.88 -0.38 0.40 -3.01 -2.93 -3.33 0.54 0.83 -3.23 -3.75 -3.90
Cytoplasm 0.78 1.63 -5.12 -5.36 -5.93 -1.58 0.88 -2.89 -1.92 -2.93 -1.48 1.42 -4.21 -2.46 -4.33
Cytoplasmic Foci -0.25 0.84 -1.63 -1.31 -2.31 -2.57 0.75 -3.61 -2.88 -3.70 -1.81 0.95 -3.93 -3.06 -4.27
Eisosomes -4.82 -14.23 -8.82 -1.50 5.19 0.23 -2.95 -3.58 -3.87 -1.51 -1.93 -9.19 -6.52 -4.54 1.58
Endoplasmic Reticulum 4.37 8.46 5.69 0.46 -7.66 -4.47 6.20 -1.85 2.23 -6.70 -0.39 10.50 2.65 3.02 -9.84
Endosome 0.09 2.92 -2.45 -2.45 -2.56 -2.40 3.26 -2.74 -2.18 -2.79 -2.37 4.26 -3.69 -2.95 -3.79
Golgi 0.48 1.23 -1.39 -1.61 -1.84 -1.36 1.21 -2.48 -2.31 -2.62 -0.99 1.60 -2.92 -2.74 -3.21
Lipid Particles 0.58 1.35 0.94 0.44 -1.85 -2.23 1.89 -1.69 -0.80 -1.74 -0.10 1.39 -0.38 -0.35 -2.43
Mitochondria -0.52 -0.80 -2.98 -4.29 -6.30 -3.00 -2.16 -5.72 -2.71 -5.39 -2.68 -1.86 -7.77 -3.89 -8.14
Mitotic Spindle -1.92 -2.05 -5.71 -0.84 -4.89 -4.82 0.81 -5.02 0.54 -5.20 -5.20 -0.52 -7.41 0.43 -7.26
None 3.35 3.45 1.76 -5.69 -6.21 -0.79 1.32 -4.42 -4.33 -5.49 2.38 3.54 -1.81 -6.40 -7.92
Nuclear Periphery -1.39 -3.58 -7.69 -7.09 -6.32 -1.42 2.36 -1.84 -1.67 -2.06 -1.86 0.57 -3.78 -3.41 -3.87
Nuclear Periphery Foci 0.08 1.45 0.21 0.08 -2.92 -0.43 1.84 -0.39 0.04 -3.06 -0.33 2.27 -0.14 0.27 -4.31
Nucleolus -5.03 11.90 6.26 8.76 -4.50 -5.19 10.59 3.32 7.23 -4.56 -6.79 15.24 5.92 10.72 -6.47
Nucleus 6.15 -7.28 1.92 -4.57 8.03 8.50 -7.91 5.76 -1.77 9.95 10.19 -10.17 6.37 -3.95 13.06
Peroxisomes -3.43 -2.02 -2.59 1.67 -0.75 -5.82 -0.84 -5.77 -1.01 -5.53 -6.34 -2.94 -7.03 -0.34 -5.13
Vacuole -2.35 -2.64 -2.87 -2.55 -2.67 -3.33 0.57 -3.16 -2.82 -3.18 -3.93 -1.10 -4.24 -3.70 -4.17
Vacuole Periphery -2.76 -1.70 -3.98 -2.07 -3.22 -0.35 7.43 -2.43 -2.34 -2.94 -1.45 4.28 -3.39 -2.89 -3.96
Standard name
Human Ortholog
Description Subunit of TFIID, TFIIF, INO80, SWI/SNF, and NuA3 complexes; involved in RNA polymerase II transcription initiation and in chromatin modification; contains a YEATS domain
Localization
Cell Percentages nucleus (99%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-2

Taf14

Taf14


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Taf14-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available