Standard name
Human Ortholog
Description Cytoplasmic protein that regulates protein phosphatase 1 Glc7p; overexpression relocalizes Glc7p from the nucleus and prevents chromosome segregation; may interact with ribosomes, based on co-purification experiments; GIP3 has a paralog, HER1, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0.07 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0.53 0.41 0.38 0.36 0.26 0.28 0.23 0.14 0.19 – 0.2 0.23 0.39 0.4 0.43 0.15 0.27 0.27 0.07 0.07 0.06 0.05 0 0
Cytoplasm 0.97 0.96 0.96 0.98 0.95 0.93 0.92 0.9 0.85 – 0.82 0.78 0.96 0.98 0.96 0.91 0.9 0.94 0.87 0.87 0.84 0.88 0.91 0.89
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0.06 0.11 0.18 – 0.24 0.28 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 1 3 1 0 – 2 1 0 0 0 1 0 0 0 4 5 2 2 2
Bud 1 10 23 3 4 4 5 14 6 – 6 6 11 5 6 2 1 1 0 0 0 0 0 2
Bud Neck 0 0 0 0 0 0 0 1 0 – 0 0 0 0 0 0 0 0 1 7 7 2 2 0
Bud Site 0 0 0 0 0 0 0 0 0 – 1 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 147 173 128 102 90 93 118 74 67 – 49 66 114 138 168 35 42 53 19 29 19 13 9 15
Cytoplasm 266 410 319 278 328 314 477 493 296 – 201 220 283 336 375 215 140 184 235 370 291 219 317 363
Endoplasmic Reticulum 4 4 3 0 3 2 8 1 4 – 3 3 2 3 3 4 9 6 3 4 7 4 4 9
Endosome 1 0 0 0 0 0 1 0 0 – 0 0 1 0 0 0 0 0 1 2 2 0 2 2
Golgi 0 0 0 0 0 0 2 0 0 – 0 0 0 0 0 3 1 0 1 1 0 0 1 0
Mitochondria 0 0 1 5 1 14 29 60 61 – 58 80 1 2 3 5 2 1 2 0 4 0 1 1
Nucleus 0 1 3 2 2 1 2 3 1 – 1 3 1 1 0 5 3 8 0 1 0 0 0 0
Nuclear Periphery 0 0 0 1 0 0 4 0 0 – 1 2 0 0 0 0 0 0 1 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 1 1 0 – 0 1 1 0 0 3 1 0 0 0 0 0 1 0
Peroxisomes 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 1
SpindlePole 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 1 0 0 0 0 0 2
Vac/Vac Membrane 0 1 0 0 4 1 3 2 0 – 1 0 0 0 0 5 4 1 1 2 2 2 1 1
Unique Cell Count 275 426 334 283 344 337 516 547 348 245 282 296 344 389 235 155 195 272 428 346 250 348 407
Labelled Cell Count 419 599 477 391 432 430 653 650 435 323 382 414 485 555 278 203 255 272 428 346 250 348 407


Cytoplasm, Endoplasmic Reticulum

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 13.7 10.0 9.9 9.2 9.7 7.8 7.4 6.5 5.9 – 6.1 5.5 10.2 10.6 9.4 11.0 12.8 12.1 11.9 12.0 12.6
Std Deviation (1e-4) 1.7 1.4 1.8 1.4 2.3 1.2 1.7 1.3 1.4 – 1.5 1.2 1.6 1.7 1.5 2.4 2.5 2.6 1.9 2.3 2.1
Intensity Change (Log2) – – – -0.1 -0.03 -0.34 -0.42 -0.61 -0.74 – -0.69 -0.86 0.04 0.1 -0.07 0.15 0.37 0.29 0.27 0.28 0.35


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud -3.6 -3.8 -3.8 -4.7 -3.1 -3.3 – -2.4 -2.8 -1.8 -3.6 -3.7 -3.5 -3.0 -3.4
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Cell Periphery -0.6 -3.4 -3.0 -4.9 -8.5 -5.5 – -4.7 -4.0 0 0.5 1.3 -6.1 -2.4 -2.6
Cytoplasm 1.9 -0.1 -1.3 -1.8 -2.9 -4.6 – -5.3 -6.5 0.1 1.6 0.6 -2.0 -2.2 -0.6
Endoplasmic Reticulum 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Mitochondria 0 0 3.4 4.1 6.1 7.8 – 9.2 10.3 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 10.1145 12.9001 12.0008 11.3803 11.2125 11.6113 10.3786 15.213 13.327 13.2238 11.3044 14.1553 11.2556 14.4313 12.5782 13.0831 12.4631 12.5707
Actin 0.0004 0.0015 0.004 0.0005 0.0013 0.0023 0.0569 0.0003 0.0123 0.0587 0.0335 0.0011 0.0085 0.0008 0.0355 0.0111 0.001 0.0011
Bud 0 0.0001 0.0002 0.0001 0.0001 0.0001 0.0013 0 0.0001 0.0005 0.0056 0.0004 0.0001 0 0.0003 0.0002 0 0.0002
Bud Neck 0.0005 0.0014 0.0024 0.0013 0.0033 0.0058 0.0021 0.0007 0.0024 0.003 0.0189 0.0081 0.0006 0.0005 0.0015 0.0025 0.0022 0.0033
Bud Periphery 0.0002 0.0001 0.0008 0.0002 0.0003 0.0003 0.0018 0.0001 0.0001 0.0008 0.0318 0.0015 0.0001 0.0001 0.0009 0.0005 0.0001 0.0005
Bud Site 0.0005 0.0061 0.0022 0.0003 0.0002 0.0002 0.012 0.0016 0.0005 0.0066 0.0051 0.0002 0.0009 0.0011 0.0017 0.0013 0.0001 0.0015
Cell Periphery 0.0267 0.0149 0.0136 0.0284 0.0042 0.0066 0.0192 0.0268 0.0261 0.0078 0.0229 0.0084 0.014 0.0271 0.0231 0.0152 0.0079 0.0277
Cytoplasm 0.9544 0.9672 0.9652 0.9642 0.9859 0.9747 0.786 0.9685 0.9348 0.8548 0.7791 0.9566 0.9031 0.9677 0.9079 0.9078 0.987 0.9525
Cytoplasmic Foci 0.0003 0.0006 0.0004 0.0001 0.0003 0.0009 0.0145 0.0001 0.0138 0.0113 0.0026 0.001 0.0168 0.0001 0.0124 0.017 0.0001 0.0008
Eisosomes 0.0002 0.0001 0.0007 0 0 0 0.0004 0 0 0.0009 0.0009 0 0.0001 0 0 0 0 0
Endoplasmic Reticulum 0.0098 0.0029 0.0036 0.0031 0.0026 0.0045 0.0159 0.0011 0.001 0.0019 0.0044 0.0061 0.0075 0.0016 0.0011 0.0063 0.0011 0.0017
Endosome 0.0001 0.0003 0.0002 0 0 0.0001 0.0192 0 0.0012 0.0146 0.0088 0.001 0.0168 0 0.0031 0.0079 0 0.0009
Golgi 0.0001 0.0002 0.0004 0 0.0001 0.0002 0.0118 0 0.0016 0.0207 0.0107 0.0002 0.0101 0.0001 0.0068 0.0118 0 0.0013
Lipid Particles 0.0013 0.0004 0.0004 0.0002 0.0001 0.0002 0.0218 0.0001 0.0018 0.0041 0.0073 0.0004 0.0112 0.0004 0.0008 0.0059 0 0.0016
Mitochondria 0 0.0008 0.0025 0 0 0 0.0032 0 0.0003 0.0045 0.0503 0.0013 0.0005 0 0.001 0.0007 0 0.0047
None 0.0039 0.0019 0.0022 0.0006 0.0006 0.0032 0.0068 0.0001 0.0002 0.0039 0.001 0.0101 0.0027 0.0001 0.0002 0.0002 0.0001 0.0002
Nuclear Periphery 0.0003 0.0003 0.0002 0.0001 0.0001 0.0002 0.0035 0.0001 0.0001 0.0014 0.0015 0.0005 0.0008 0.0001 0.0001 0.0002 0 0.0001
Nucleolus 0 0.0001 0 0 0 0 0.0005 0 0 0.0001 0.0006 0.0001 0 0 0 0 0 0
Nucleus 0.0003 0.0004 0.0003 0.0003 0.0003 0.0003 0.0117 0.0001 0.0001 0.0003 0.0011 0.0016 0.0002 0.0001 0.0002 0.0002 0.0001 0.0001
Peroxisomes 0 0 0 0 0 0 0.0054 0 0.0029 0.0027 0.0022 0 0.0044 0 0.0029 0.0097 0 0.0014
Punctate Nuclear 0 0.0003 0 0 0 0.0001 0.0024 0 0.0005 0.0007 0.0003 0.0001 0.0007 0 0.0001 0.0004 0 0
Vacuole 0.0011 0.0005 0.0005 0.0004 0.0003 0.0002 0.0029 0.0003 0.0002 0.0004 0.0095 0.0011 0.0006 0.0002 0.0004 0.001 0.0001 0.0003
Vacuole Periphery 0 0 0 0 0 0 0.0006 0 0 0.0003 0.0019 0.0001 0.0002 0 0 0 0 0

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 35.8585 24.7173 26.5567 36.7052 22.0324 39.4369 38.659 41.6421 39.6216 31.1305
Translational Efficiency 0.6396 0.5969 0.4902 0.4331 0.574 0.6551 0.4029 0.3964 0.4202 0.4269

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1864 934 171 1196 1791 1368 139 1116 3655 2302 310 2312

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 939.97 1113.03 1561.26 1340.62 946.32 1012.62 1377.11 1313.81 943.08 1053.36 1478.69 1327.68
Standard Deviation 117.22 235.70 178.92 185.98 118.20 145.99 146.96 198.05 117.74 194.00 189.03 192.37
Intensity Change Log 2 — 0.243806 0.732024 0.512214 — 0.097693 0.541244 0.473357 — 0.172353 0.639465 0.492851

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.005846 0.009986 0.041692 0.010842 0.007237 0.006713 0.006226 0.022382 0.006528 0.008041 0.025790 0.016412
Bud Neck 0.029573 0.018525 0.018865 0.006011 0.027595 0.019011 0.006511 0.007944 0.028603 0.018814 0.013325 0.006944
Bud Site 0.076326 0.083567 0.135400* 0.076118 0.070077 0.068895 0.073898 0.092064 0.073264 0.074848 0.107824* 0.083815
Cell Periphery 0.024731 0.012558 0.035287 0.001543 0.021563 0.005084 0.004434 0.005265 0.023178 0.008117 0.021453 0.003339
Cytoplasm 0.700601* 0.630992* 0.274336* 0.643928* 0.699069* 0.625062* 0.217100* 0.603533* 0.699851* 0.627468* 0.248672* 0.624429*
Cytoplasmic Foci 0.041483 0.085048 0.108303 0.038795 0.048842 0.099647 0.118713 0.037378 0.045089 0.093724 0.112970 0.038111
Eisosomes 0.008950 0.004447 0.001604 0.000217 0.012617 0.002522 0.000864 0.000568 0.010747 0.003303 0.001272 0.000387
Endoplasmic Reticulum 0.002884 0.004860 0.009127 0.002834 0.003486 0.002104 0.003261 0.000984 0.003179 0.003222 0.006497 0.001941
Endosome 0.002032 0.004133 0.008087 0.008179 0.003579 0.008431 0.008979 0.008780 0.002790 0.006687 0.008487 0.008469
Golgi 0.004194 0.004534 0.003859 0.004775 0.007075 0.005808 0.000421 0.003861 0.005606 0.005291 0.002317 0.004334
Lipid Particles 0.003535 0.006411 0.047931 0.004014 0.004200 0.004254 0.007370 0.007533 0.003861 0.005129 0.029744 0.005713
Mitochondria 0.000859 0.000710 0.000014 0.002181 0.001810 0.000997 0.000007 0.001897 0.001325 0.000881 0.000011 0.002044
Mitotic Spindle 0.000327 0.003285 0.000321 0.025152 0.000757 0.004193 0.000088 0.013316 0.000538 0.003825 0.000216 0.019439
None 0.005170 0.002104 0.000053 0.000305 0.003952 0.001691 0.000030 0.000406 0.004573 0.001859 0.000043 0.000354
Nuclear Periphery 0.000088 0.000056 0.000139 0.000189 0.000059 0.000055 0.000130 0.000142 0.000074 0.000055 0.000135 0.000166
Nuclear Periphery Foci 0.002478 0.005305 0.032214 0.002745 0.001467 0.002456 0.016834 0.003734 0.001983 0.003612 0.025318 0.003223
Nucleolus 0.000375 0.000247 0.000023 0.000154 0.000420 0.000294 0.000025 0.000073 0.000397 0.000275 0.000024 0.000115
Nucleus 0.032187 0.026022 0.011656 0.012699 0.037782 0.026296 0.008176 0.018619 0.034929 0.026185 0.010096 0.015556
Peroxisomes 0.002219 0.002548 0.001059 0.004959 0.002296 0.004108 0.001254 0.002462 0.002257 0.003475 0.001146 0.003754
Vacuole 0.055742 0.094139 0.269854* 0.151683 0.045447 0.111830 0.525595* 0.165472 0.050697 0.104652 0.384525* 0.158339
Vacuole Periphery 0.000399 0.000523 0.000176 0.002679 0.000672 0.000547 0.000086 0.003586 0.000533 0.000537 0.000135 0.003116

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -4.21 -6.35 -2.45 1.22 5.74 0.82 1.57 -5.45 -5.52 -5.70 -2.40 -5.71 -5.86 -3.86 3.60
Bud Neck 7.60 7.71 22.84 12.81 12.83 6.14 20.04 20.06 11.47 -0.35 9.60 15.90 30.39 16.93 10.00
Bud Site -1.71 -6.47 5.48 6.12 8.59 0.59 0.71 -0.12 -0.62 -0.74 -0.28 -5.08 3.61 3.35 6.58
Cell Periphery 10.13 -1.19 25.28* 14.10 7.25 17.07 13.80 18.98 3.63 0.21 20.09 2.22 31.32 13.87 6.75
Cytoplasm 9.61 28.78 15.09 6.42 -17.51 10.67 31.40 16.07 6.98 -19.74 14.38 41.79 22.01 9.31 -25.87
Cytoplasmic Foci -13.28 -10.76 2.68 14.68 11.63 -14.15 -6.57 5.85 17.90 8.10 -19.45 -12.00 6.00 22.99 13.81
Eisosomes 9.24 17.98 22.42 14.03 7.90 18.96 23.34 24.48 10.38 3.14 20.88 29.10 32.99 17.36 8.17
Endoplasmic Reticulum -1.65 -4.00 1.83 2.85 4.97 2.72 1.23 6.91 3.88 6.58 -0.01 -3.35 4.33 3.69 6.18
Endosome -5.98 -8.12 -1.82 0.85 4.20 -6.09 -5.09 -0.92 3.56 2.87 -8.12 -9.20 -1.86 3.84 4.98
Golgi -0.87 2.52 3.89 4.10 2.16 2.07 26.08 8.72 4.67 -2.54 1.01 14.36 8.75 6.05 0.43
Lipid Particles -3.01 -8.94 -1.64 2.20 8.77 -0.08 -1.93 -2.71 -2.43 0.08 -2.75 -8.43 -3.15 -0.08 7.89
Mitochondria 0.44 7.42 0.21 -0.16 -2.09 1.41 3.28 1.37 0.27 -1.90 1.38 4.78 1.30 0.16 -2.80
Mitotic Spindle -2.26 0.22 -4.47 -2.64 -4.49 -2.70 1.52 -3.03 -1.08 -3.49 -3.61 1.48 -5.38 -2.68 -5.67
None 4.21 11.00 10.50 3.14 -6.90 5.05 9.97 9.12 6.24 -6.46 6.75 14.82 13.90 5.81 -9.24
Nuclear Periphery 1.22 -2.12 -1.51 -3.39 0.13 0.08 -5.80 -5.35 -4.63 0.26 1.17 -4.30 -3.10 -5.09 0.28
Nuclear Periphery Foci -8.54 -12.56 -0.41 7.76 12.50 -7.12 -9.76 -8.21 -2.99 8.39 -9.98 -14.88 -5.96 3.71 14.22
Nucleolus 1.58 6.42 4.35 2.26 -1.73 0.63 2.28 2.12 5.57 -2.96 1.19 4.16 3.56 5.16 -2.33
Nucleus 2.86 11.77 9.61 4.06 -3.63 6.58 19.18 10.87 4.79 -9.14 6.60 20.92 14.51 6.49 -8.08
Peroxisomes -1.10 6.69 -0.35 0.18 -2.53 -3.56 3.78 3.14 4.64 -1.35 -3.70 7.02 0.76 3.31 -2.90
Vacuole -10.20 -16.11 -29.98 -22.60 1.02 -17.13 -26.14 -29.24 -18.17 13.79 -19.48 -25.74 -41.89 -28.31 9.27
Vacuole Periphery -0.54 4.52 -1.38 -0.98 -2.00 0.57 2.81 -1.43 -1.71 -2.49 0.03 3.83 -1.95 -1.96 -3.18
Standard name
Human Ortholog
Description Cytoplasmic protein that regulates protein phosphatase 1 Glc7p; overexpression relocalizes Glc7p from the nucleus and prevents chromosome segregation; may interact with ribosomes, based on co-purification experiments; GIP3 has a paralog, HER1, that arose from the whole genome duplication
Localization
Cell Percentages cytoplasm (90%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-10

Gip3

Gip3


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Gip3-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available