Standard name
Human Ortholog
Description Vacuolar aspartyl protease (proteinase A); required for posttranslational precursor maturation of vacuolar proteinases; important for protein turnover after oxidative damage; plays a protective role in acetic acid induced apoptosis; synthesized as a zymogen, self-activates
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.11 0.23 0.19 0.21 0.11 0.13 0.2 0.26 0.28 0.27 0.35 0.31 – 0.56 0.78 0.1 0.14 0.12 0 0.05 0 0 0.05 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0.05 0.06 0.07 0 0 0 0 0 0
Endosome 0 0 0.11 0.05 0 0 0 0 0 0 0 0 – 0.06 0 0 0 0 0 0 0 0.05 0.08 0.06
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.6 0.13 0.07 0 0.1 0.14 0.12 0.06 0.05 0.1 0.12 0.1 – 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0.18 0.09 0.14 0.26 0.29 0.28 0.29 0.29 0.18 0.22 0.14 0.13 – 0.09 0 0.47 0.41 0.36 0 0 0 0.08 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.51 0.71 0.74 0.72 0.7 0.68 0.66 0.7 0.75 0.72 0.69 0.72 – 0.4 0.31 0.54 0.54 0.66 0.88 0.87 0.91 0.79 0.74 0.77
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 1 0 0 2 0 2 1 0 0 – 0 0 0 0 0 1 0 1 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 9 27 44 40 29 53 85 107 90 123 86 109 – 68 80 10 9 7 1 5 3 1 3 3
Endoplasmic Reticulum 1 1 1 1 0 0 0 1 0 1 0 1 – 3 0 5 4 4 0 0 2 0 0 0
Endosome 0 4 26 10 8 2 0 2 0 0 0 1 – 7 2 0 1 0 0 3 2 3 5 5
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 1 0 0 0 0 1 0 0 0
Mitochondria 51 15 16 8 27 55 51 25 16 45 30 34 – 0 0 4 1 0 1 1 2 0 3 3
Nucleus 15 11 32 49 78 110 124 119 57 97 35 45 – 11 2 48 26 21 3 0 0 5 2 2
Nuclear Periphery 2 3 1 1 3 6 4 1 4 8 1 5 – 0 0 1 0 1 0 0 1 0 1 1
Nucleolus 0 1 1 0 2 2 0 0 0 3 2 4 – 0 0 4 1 1 0 1 1 0 0 1
Peroxisomes 0 0 0 0 1 1 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 1 1 2 2 1 0 0 0 0 0 1 – 0 0 1 0 0 0 1 1 1 1 0
Vac/Vac Membrane 43 83 172 138 185 267 285 290 242 324 170 256 – 49 32 55 34 38 80 111 226 58 55 77
Unique Cell Count 85 117 232 191 265 394 432 414 324 448 245 355 121 102 102 63 58 92 127 249 74 76 101
Labelled Cell Count 121 146 294 250 335 497 551 545 411 602 324 456 138 116 129 76 72 92 127 249 74 76 101


Vacuole

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.8 5.2 5.4 5.8 6.6 6.1 6.2 6.2 6.3 6.0 5.8 6.4 – 6.2 5.6 9.7 10.1 9.3 5.4 6.9 7.1
Std Deviation (1e-4) 0.7 1.4 1.7 2.2 1.8 1.9 1.8 1.8 1.8 1.7 1.7 2.1 – 1.5 1.5 3.0 3.5 3.1 0.9 1.6 1.4
Intensity Change (Log2) – – – 0.08 0.27 0.17 0.19 0.18 0.21 0.15 0.1 0.24 – 0.19 0.04 0.83 0.89 0.77 -0.01 0.34 0.38


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0
Cytoplasm 0.5 -2.5 -1.8 0.2 2.0 2.4 2.4 4.0 3.2 – 7.1 10.4 -2.1 -0.9 -1.2
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0
Endosome -2.2 -3.6 -6.3 -7.1 -6.4 -6.2 -7.2 -5.4 -6.2 – -1.7 -2.8 -3.5 -2.3 -2.7
Golgi 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0
Mitochondria -1.2 1.3 2.7 2.0 -0.4 -1.0 1.4 2.0 1.1 – -3.0 0 -1.1 0 0
Nucleus 3.1 4.2 4.1 4.3 4.3 1.2 2.5 0.2 -0.4 – -1.3 -3.3 6.6 4.9 4.0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0
Vacuole -0.4 -1.1 -1.7 -2.2 -1.1 0.1 -0.5 -1.2 -0.5 – -6.2 -7.4 -3.6 -3.1 -1.3

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.629 3.0252 1.4138 1.6442 2.2505 2.4508 2.3689 2.6418 2.0908 1.8593 2.5753 2.2541 0.9878 2.5306 2.2332 1.9524 2.2793 2.7861
Actin 0.0026 0 0.0001 0 0.0505 0.0013 0.0584 0.0001 0.0043 0.0019 0.0311 0.0002 0.0521 0 0.0293 0.0566 0.0246 0
Bud 0.0002 0.0002 0.0002 0.0002 0.0004 0.0003 0.0012 0.0003 0.0015 0.0003 0.0025 0.0006 0.0009 0.0003 0.0006 0.0002 0.0003 0.0003
Bud Neck 0.0098 0.0004 0.0008 0.0007 0.0012 0.0017 0.0038 0.0011 0.0043 0.001 0.0034 0.0026 0.0022 0.0017 0.0017 0.0012 0.0025 0.0013
Bud Periphery 0.0007 0.0007 0.0008 0.0006 0.0007 0.0014 0.0023 0.0009 0.0029 0.0009 0.0058 0.0013 0.0023 0.0008 0.0016 0.0006 0.0007 0.001
Bud Site 0.0021 0.0005 0.0006 0.0005 0.0011 0.0008 0.0063 0.0012 0.0049 0.0008 0.0047 0.001 0.0021 0.0008 0.002 0.0006 0.0007 0.0005
Cell Periphery 0.0036 0.0046 0.0051 0.0024 0.0024 0.0059 0.0024 0.0039 0.0037 0.0023 0.0045 0.0037 0.0042 0.0055 0.0056 0.0032 0.0044 0.0059
Cytoplasm 0.0023 0.0007 0.001 0.0008 0.0048 0.0007 0.0064 0.0079 0.0053 0.0016 0.0077 0.0008 0.0132 0.0005 0.0009 0.0004 0.0019 0.0005
Cytoplasmic Foci 0.0101 0.0011 0.019 0.0019 0.0386 0.0017 0.0201 0.0025 0.0094 0.0065 0.0105 0.0051 0.0239 0.0036 0.0096 0.0057 0.0256 0.0014
Eisosomes 0.0002 0 0 0 0.0002 0.0001 0.001 0 0.0002 0 0.0005 0 0.0004 0 0.0004 0.0001 0.0001 0
Endoplasmic Reticulum 0.0013 0.0012 0.0014 0.0006 0.0015 0.0008 0.0084 0.0022 0.0031 0.0019 0.0046 0.0015 0.0058 0.0007 0.002 0.0003 0.0067 0.0006
Endosome 0.1207 0.0695 0.1045 0.1327 0.1574 0.0612 0.1897 0.0782 0.1534 0.131 0.1429 0.1087 0.0804 0.0544 0.1021 0.1021 0.0804 0.0574
Golgi 0.0056 0.0019 0.0065 0.0028 0.0134 0.0047 0.0235 0.0032 0.0123 0.0107 0.0272 0.0043 0.0135 0.002 0.0111 0.0046 0.012 0.0014
Lipid Particles 0.0497 0.0182 0.0297 0.0118 0.0418 0.0352 0.0454 0.0124 0.0297 0.0234 0.0206 0.0144 0.0751 0.0193 0.0281 0.051 0.0833 0.0159
Mitochondria 0.0358 0.0107 0.0576 0.0407 0.0037 0.03 0.0596 0.0193 0.0752 0.0092 0.046 0.0075 0.0574 0.0143 0.067 0.0015 0.0036 0.0009
None 0.0107 0.0004 0.0018 0.0001 0.0072 0.0001 0.0219 0.0008 0.0006 0.0002 0.0016 0.0002 0.0169 0.0001 0.0005 0.0001 0.0007 0.0001
Nuclear Periphery 0.0028 0.0061 0.0238 0.0013 0.0069 0.0038 0.0385 0.0034 0.0227 0.0028 0.0161 0.0105 0.0249 0.0016 0.0076 0.0007 0.0408 0.0013
Nucleolus 0.0057 0.007 0.0073 0.0033 0.0034 0.0068 0.007 0.0116 0.0091 0.0029 0.0105 0.0102 0.0113 0.011 0.0088 0.0064 0.0039 0.01
Nucleus 0.0029 0.0052 0.0126 0.0018 0.0043 0.0198 0.0106 0.0046 0.008 0.0025 0.0253 0.0087 0.012 0.0033 0.0225 0.002 0.0051 0.0041
Peroxisomes 0.0028 0.0005 0.0059 0.0007 0.0288 0.0015 0.0105 0.0007 0.0048 0.0129 0.0035 0.001 0.028 0.0022 0.014 0.0024 0.0191 0.0007
Punctate Nuclear 0.0063 0.0002 0.0006 0 0.0223 0.0001 0.0078 0.0002 0.0005 0.0128 0.0034 0.0009 0.0082 0.0001 0.0029 0.0001 0.0184 0
Vacuole 0.4888 0.6019 0.4532 0.5979 0.4651 0.6106 0.3203 0.6125 0.4423 0.6217 0.4969 0.6292 0.3769 0.625 0.4628 0.6054 0.5343 0.7183
Vacuole Periphery 0.2354 0.2689 0.2675 0.1991 0.1441 0.2111 0.1548 0.2328 0.2019 0.1527 0.1308 0.1873 0.1884 0.253 0.219 0.1548 0.131 0.1786

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 393.1671 347.4182 348.5398 358.2313 234.9117 522.4236 469.3891 444.6061 518.9724 416.1761
Translational Efficiency 1.1749 1.2004 1.2278 1.3873 1.3705 1.0957 1.2572 1.383 1.3348 1.4277

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Vacuolar aspartyl protease (proteinase A); required for posttranslational precursor maturation of vacuolar proteinases; important for protein turnover after oxidative damage; plays a protective role in acetic acid induced apoptosis; synthesized as a zymogen, self-activates
Localization
Cell Percentages vacuole (84%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Pep4

Pep4


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Pep4-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available