Standard name
Human Ortholog
Description Protein involved in establishment of cell polarity and bud emergence; interacts with the Rho1p small GTP-binding protein and with the Rho-type GTPase Cdc42p; involved in maintenance of proper telomere length
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.98 0.99 0.99 0.98 0.91 0.86 0.71 0.69 – 0.73 0.69 0.65 0.96 0.99 0.99 0.95 0.97 0.97 0.95 0.97 0.97 0.92 0.91 0.88
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0.06 0.13 0.26 0.45 0.43 – 0.38 0.52 0.51 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0.05 – 0.08 0.05 0 0 0 0 0.06 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0
Bud 0 0 1 1 2 4 4 4 – 9 15 8 1 0 0 0 1 0 0 0 0 0 3 5
Bud Neck 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1
Bud Site 0 0 0 0 0 0 0 1 – 2 2 1 0 0 0 0 0 0 – – – – – –
Cell Periphery 1 0 0 0 1 2 0 1 – 3 2 5 1 1 0 6 6 5 0 0 0 0 0 0
Cytoplasm 235 93 198 245 384 549 502 469 – 444 357 499 270 406 453 236 290 268 229 94 194 142 277 308
Endoplasmic Reticulum 1 0 1 0 1 4 4 0 – 1 0 2 4 2 1 8 9 5 1 0 0 2 4 5
Endosome 0 0 0 1 0 0 0 0 – 0 0 0 0 0 0 1 1 0 1 0 1 2 0 4
Golgi 1 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 1 1 0 0 0 0 1
Mitochondria 1 1 3 15 57 167 313 294 – 232 268 395 0 0 0 0 2 1 2 0 1 0 1 6
Nucleus 0 0 1 0 2 3 10 10 – 10 3 14 6 3 2 6 3 4 0 0 0 2 2 2
Nuclear Periphery 1 0 1 0 1 1 8 7 – 3 0 1 0 0 0 0 0 0 0 0 0 1 0 2
Nucleolus 0 0 0 1 0 2 0 0 – 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 – 0 2 0 0 0 0 0 0 0 0 0 0 0 1 2
Vac/Vac Membrane 2 0 0 5 19 7 24 36 – 51 26 12 6 5 6 14 4 5 2 0 0 2 8 5
Unique Cell Count 240 94 201 250 423 635 703 682 611 518 769 281 410 459 249 298 275 242 97 201 155 306 351
Labelled Cell Count 242 94 205 268 467 739 865 822 756 675 937 288 417 462 271 316 289 242 97 201 155 306 351


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 7.1 6.8 4.8 4.7 4.5 4.1 3.6 3.9 – 3.8 3.7 3.6 7.1 7.1 7.3 9.1 9.3 9.1 8.1 7.6 7.7
Std Deviation (1e-4) 0.8 0.8 0.5 1.2 1.2 0.8 0.7 0.8 – 0.8 0.9 0.6 1.2 1.1 1.1 1.2 1.3 1.2 1.9 1.3 1.5
Intensity Change (Log2) – – – -0.02 -0.09 -0.2 -0.41 -0.31 – -0.32 -0.38 -0.41 0.58 0.57 0.62 0.93 0.97 0.93 0.76 0.68 0.69


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Cytoplasm -0.4 -3.6 -4.8 -8.1 -8.6 – -7.8 -8.5 -9.4 -1.6 0.6 0.2 -2.1 -0.9 -0.8
Endoplasmic Reticulum 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Vacuole 0 3.1 0 2.7 3.3 – 4.2 3.2 0 0 0 0 3.4 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration – – – – – – – – – – – – – – – – – –
Actin 0.0006 0.0006 0.0014 0.0001 0.0004 0.0013 0.0053 0.001 0.0006 0.0517 0.0007 0.0006 0.0084 0.0002 0.0276 0.0005 0.0014 0.0002
Bud 0.0008 0.0024 0.004 0.0001 0.0001 0.0009 0.0011 0.0002 0.0006 0.0003 0.0002 0.0001 0.0003 0.0001 0.0004 0.0001 0.0003 0
Bud Neck 0.0002 0.0006 0.0005 0.0007 0.001 0.0013 0.0015 0.0002 0.0036 0.0008 0.0004 0.0006 0.0001 0 0.0001 0.0015 0.0001 0.0004
Bud Periphery 0.0004 0.0007 0.0018 0.0001 0 0.0005 0.0009 0.0001 0.0005 0.0005 0.0001 0 0.0003 0 0.0004 0 0.0007 0
Bud Site 0.0004 0.0152 0.0056 0.0001 0.0002 0.0002 0.0115 0.0009 0.0051 0.0004 0.0001 0.0001 0.0003 0.0005 0.0023 0 0.0003 0
Cell Periphery 0 0.0001 0.0001 0 0 0 0.0003 0.0001 0.0001 0.0005 0 0 0.0001 0 0.0004 0 0.0002 0
Cytoplasm 0.7562 0.7844 0.7996 0.8286 0.6949 0.8144 0.6532 0.7822 0.7646 0.7943 0.7623 0.8157 0.7302 0.9327 0.7127 0.9761 0.0014 0.9536
Cytoplasmic Foci 0.0088 0.0105 0.0099 0.016 0.0389 0.0105 0.0102 0.006 0.0051 0.0049 0.0096 0.0083 0.0032 0.0015 0.0058 0.0034 0.0087 0.004
Eisosomes 0 0 0 0 0 0 0.0001 0 0.0001 0.0003 0 0 0 0 0.0003 0 0.0003 0
Endoplasmic Reticulum 0.0025 0.0016 0.0024 0.0018 0.002 0.0017 0.0042 0.0029 0.0025 0.0034 0.0023 0.002 0.0246 0.0015 0.0134 0.001 0.0014 0.0025
Endosome 0.005 0.0044 0.0039 0.0089 0.0043 0.0032 0.0054 0.0021 0.0025 0.0019 0.002 0.0061 0.0306 0.0003 0.005 0.0004 0.0755 0.0022
Golgi 0.0002 0.0002 0.0002 0.0002 0.0003 0.0001 0.0013 0.0004 0.0001 0.0022 0.0002 0.0002 0.0022 0 0.0033 0 0.0383 0.0001
Lipid Particles 0.0001 0.0002 0.0001 0 0.0001 0 0.0011 0.0001 0.0002 0.0003 0.0001 0.0001 0.0005 0 0.0008 0 0.0088 0
Mitochondria 0.0003 0.0004 0.0004 0.0005 0.0003 0.0003 0.0003 0.0002 0.0002 0.0003 0.0002 0.0002 0.0025 0 0.0006 0 0.8446 0.0001
None 0.2105 0.1494 0.1481 0.0901 0.2149 0.1387 0.2953 0.1962 0.2082 0.1347 0.2165 0.1545 0.139 0.0606 0.2222 0.0152 0.0014 0.0346
Nuclear Periphery 0.0015 0.0017 0.0016 0.0019 0.0021 0.0014 0.0013 0.0008 0.0011 0.0005 0.0008 0.003 0.0452 0.0007 0.0021 0.0002 0.0007 0.0006
Nucleolus 0.0001 0.0004 0.0004 0.0002 0.0001 0.0001 0.0002 0.0001 0.0001 0 0 0.0001 0.0001 0 0 0 0.0002 0
Nucleus 0.0086 0.0195 0.0128 0.0129 0.0292 0.0201 0.0025 0.0048 0.0023 0.0021 0.0022 0.0047 0.0068 0.0013 0.0009 0.0008 0.0003 0.0013
Peroxisomes 0.0002 0.0003 0.0002 0.0003 0.0025 0.0006 0.0008 0.0001 0.0001 0.0001 0.0002 0.0001 0.0002 0 0.0002 0 0.0054 0
Punctate Nuclear 0.0024 0.0061 0.0061 0.0351 0.0076 0.0038 0.0027 0.0008 0.0016 0.0004 0.0015 0.0027 0.0011 0.0001 0.0006 0.0002 0.0001 0.0002
Vacuole 0.001 0.0012 0.0009 0.0018 0.0008 0.0007 0.0008 0.0008 0.0007 0.0005 0.0004 0.0006 0.0021 0.0004 0.0006 0.0002 0.0035 0.0003
Vacuole Periphery 0.0001 0.0001 0.0001 0.0004 0.0001 0.0001 0.0001 0.0001 0.0001 0.0001 0.0001 0.0002 0.0023 0 0.0002 0 0.0064 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 7.8317 6.7418 6.1572 10.8669 7.5216 6.3779 11.798 10.7941 9.6062 11.6276
Translational Efficiency 1.0268 1.1125 1.128 0.8481 1.0002 1.3077 0.6872 0.6315 0.8202 0.7823

Hit Data

Dataset Hit
Protein Concentration –
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1139 1310 158 1362 1507 781 2093 1693 2646 2091 2251 3055

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 771.13 818.76 1100.20 1026.25 773.87 796.76 1011.03 1053.89 772.69 810.54 1017.29 1041.57
Standard Deviation 93.56 160.91 128.38 131.30 97.64 113.72 125.63 132.60 95.92 145.49 127.87 132.73
Intensity Change Log 2 — 0.086467 0.512720 0.412336 — 0.042054 0.385663 0.445561 — 0.064392 0.450477 0.429074

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000142 0.000304 0.000878 0.001491 0.000140 0.000304 0.000116 0.001887 0.000141 0.000304 0.000169 0.001711
Bud Neck 0.004403 0.006964 0.003934 0.008276 0.002718 0.006826 0.002664 0.004852 0.003443 0.006912 0.002754 0.006379
Bud Site 0.003807 0.010274 0.008739 0.048315 0.003933 0.011149 0.004419 0.025024 0.003879 0.010601 0.004722 0.035408
Cell Periphery 0.000113 0.000120 0.000310 0.000152 0.000085 0.000076 0.000049 0.000101 0.000097 0.000103 0.000067 0.000124
Cytoplasm 0.648140* 0.639291* 0.427431* 0.568269* 0.701657* 0.670090* 0.607823* 0.599680* 0.678620* 0.650795* 0.595161* 0.585676*
Cytoplasmic Foci 0.159284 0.130075 0.018056 0.015672 0.123232 0.139895 0.004506 0.013172 0.138751 0.133743 0.005457 0.014287
Eisosomes 0.000066 0.000045 0.000072 0.000028 0.000041 0.000047 0.000010 0.000027 0.000052 0.000046 0.000014 0.000027
Endoplasmic Reticulum 0.000703 0.001171 0.007335 0.002168 0.000823 0.000650 0.001032 0.003009 0.000771 0.000976 0.001474 0.002634
Endosome 0.010509 0.011099 0.007104 0.007929 0.006196 0.010645 0.000631 0.004916 0.008053 0.010929 0.001086 0.006259
Golgi 0.001024 0.002054 0.000248 0.006911 0.001107 0.001556 0.000089 0.003185 0.001071 0.001868 0.000100 0.004846
Lipid Particles 0.002942 0.002234 0.003265 0.001053 0.002272 0.002403 0.000364 0.000741 0.002560 0.002297 0.000568 0.000880
Mitochondria 0.002617 0.002994 0.000454 0.003167 0.001771 0.004213 0.000478 0.005282 0.002135 0.003449 0.000476 0.004339
Mitotic Spindle 0.000656 0.008559 0.006646 0.043847 0.001243 0.002013 0.008061 0.038042 0.000990 0.006114 0.007962 0.040630
None 0.003045 0.003688 0.003147 0.007214 0.002676 0.001793 0.007700 0.013903 0.002835 0.002980 0.007381 0.010921
Nuclear Periphery 0.000197 0.000321 0.002014 0.000930 0.000207 0.000177 0.001012 0.000617 0.000203 0.000267 0.001082 0.000757
Nuclear Periphery Foci 0.001376 0.001315 0.010884 0.002914 0.001124 0.000820 0.001211 0.001384 0.001233 0.001130 0.001890 0.002066
Nucleolus 0.000609 0.001904 0.000349 0.000871 0.000549 0.000393 0.000144 0.000400 0.000575 0.001340 0.000159 0.000610
Nucleus 0.104809 0.077221 0.316767* 0.157299* 0.094173 0.065219 0.322382* 0.203644* 0.098752 0.072739 0.321988* 0.182983*
Peroxisomes 0.001420 0.002945 0.000359 0.001383 0.001027 0.003250 0.000052 0.001744 0.001196 0.003059 0.000073 0.001583
Vacuole 0.053646 0.095819 0.181693 0.118499 0.054416 0.077433 0.036817 0.074964 0.054084 0.088951 0.046986 0.094373
Vacuole Periphery 0.000492 0.001604 0.000317 0.003610 0.000609 0.001048 0.000440 0.003427 0.000559 0.001396 0.000431 0.003509

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -3.26 -9.18 -4.05 -2.99 1.90 -2.41 -1.40 -2.22 -1.64 -2.09 -4.19 -4.10 -3.57 -2.64 -3.00
Bud Neck -2.36 -0.23 -1.01 1.68 -0.86 -3.70 -0.00 -1.64 2.77 -1.75 -4.64 1.42 -1.91 3.39 -3.50
Bud Site -5.19 -3.39 -6.51 -3.57 -4.07 -3.74 0.21 -4.82 -0.11 -4.73 -6.39 -0.28 -8.08 -2.68 -7.40
Cell Periphery -0.12 -7.91 -0.09 0.09 7.95 1.69 6.66 1.49 -0.31 -4.58 -0.30 5.11 0.73 0.57 -4.46
Cytoplasm 1.71 16.66 19.73 17.44 -6.56 3.08 23.18 23.66 15.16 2.80 4.59 25.29 30.77 22.36 4.17
Cytoplasmic Foci 4.90 27.37 29.60 26.35 1.70 -2.53 32.78 31.12 22.58 -6.90 1.46 44.58 42.63 34.80 -9.34
Eisosomes 6.36 -2.11 9.59 3.16 7.06 -1.91 13.32 5.61 6.30 -7.29 2.95 17.54 10.60 7.04 -8.63
Endoplasmic Reticulum -3.05 -10.07 -11.24 -6.41 8.07 1.62 -7.78 -2.08 -2.40 -0.61 -1.95 -12.13 -4.34 -3.60 0.10
Endosome -0.12 2.78 6.06 8.71 4.71 -3.59 11.84 7.43 7.32 -3.51 -2.96 11.83 8.98 11.75 -4.33
Golgi -2.32 5.06 -0.86 0.52 -2.15 -0.89 4.95 0.51 1.20 -2.98 -2.30 7.20 -0.39 1.35 -3.58
Lipid Particles 1.57 -0.65 3.20 3.10 5.81 -0.27 8.42 6.41 2.81 -4.35 0.93 7.78 6.02 4.47 -6.57
Mitochondria -0.14 1.81 1.26 1.86 -2.41 -1.36 1.95 -0.12 1.34 -2.67 -1.15 2.59 0.88 2.04 -3.33
Mitotic Spindle -4.09 -2.00 -7.77 -3.71 -3.89 -0.81 -5.11 -7.01 -6.53 -3.89 -4.02 -5.77 -10.36 -6.14 -5.53
None -0.76 0.15 -5.10 -3.93 -4.79 2.91 -13.33 -8.48 -9.73 -2.50 -0.10 -12.94 -9.91 -9.08 -1.55
Nuclear Periphery -4.47 -13.68 -23.37 -20.20 3.88 1.13 -29.71 -22.70 -21.98 5.91 -3.41 -32.69 -32.14 -28.05 2.88
Nuclear Periphery Foci 0.25 -7.76 -4.93 -6.46 6.57 2.46 -3.02 -2.04 -4.29 1.38 0.70 -5.21 -5.38 -6.60 1.08
Nucleolus -2.17 2.21 1.05 2.48 -0.56 1.32 3.03 1.33 0.17 -3.23 -1.98 4.53 1.67 2.54 -2.99
Nucleus 5.69 -11.90 -18.29 -22.94 6.42 6.72 -47.71 -28.92 -32.49 12.15 7.90 -50.32 -33.85 -38.77 18.90
Peroxisomes -3.22 5.84 4.48 5.75 -1.12 -3.26 3.77 1.36 4.09 -3.53 -4.67 6.66 3.30 6.85 -4.65
Vacuole -11.32 -10.39 -26.75 -17.97 0.02 -5.22 -5.11 -18.21 -12.10 -14.23 -12.32 -9.02 -31.38 -19.86 -21.16
Vacuole Periphery -3.77 2.97 -1.24 1.57 -1.95 -1.82 2.05 -1.02 0.04 -1.86 -4.03 2.00 -1.52 1.21 -2.42
Standard name
Human Ortholog
Description Protein involved in establishment of cell polarity and bud emergence; interacts with the Rho1p small GTP-binding protein and with the Rho-type GTPase Cdc42p; involved in maintenance of proper telomere length
Localization
Cell Percentages cytoplasm (85%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Bem4

Bem4


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Bem4-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available