Standard name | |
---|---|
Human Ortholog | |
Description | Cell wall and vacuolar protein; required for wild-type resistance to vanadate; SVS1 has a paralog, SRL1, that arose from the whole genome duplication |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 3 | 7 | 12 | 6 | 1 | 7 | 3 | 2 | 1 | 1 | 3 | 4 | 11 | 11 | – | – | – | 6 | 17 | 27 | 4 | 9 | 8 |
Bud | 0 | 7 | 3 | 10 | 7 | 6 | 9 | 7 | 19 | 5 | 16 | 18 | 2 | 9 | 5 | – | – | – | 6 | 5 | 16 | 5 | 11 | 9 |
Bud Neck | 0 | 3 | 14 | 7 | 10 | 5 | 15 | 18 | 6 | 8 | 11 | 6 | 2 | 9 | 6 | – | – | – | 10 | 24 | 55 | 24 | 18 | 16 |
Bud Site | 0 | 0 | 4 | 5 | 0 | 1 | 3 | 2 | 4 | 2 | 6 | 3 | 0 | 2 | 0 | – | – | – | – | – | – | – | – | – |
Cell Periphery | 0 | 2 | 7 | 8 | 5 | 4 | 3 | 4 | 5 | 2 | 1 | 7 | 0 | 3 | 3 | – | – | – | 1 | 2 | 10 | 3 | 4 | 3 |
Cytoplasm | 25 | 205 | 313 | 254 | 470 | 398 | 540 | 595 | 417 | 433 | 347 | 426 | 69 | 183 | 226 | – | – | – | 16 | 106 | 116 | 65 | 142 | 152 |
Endoplasmic Reticulum | 3 | 0 | 3 | 3 | 3 | 2 | 1 | 0 | 0 | 0 | 0 | 6 | 1 | 0 | 2 | – | – | – | 3 | 0 | 5 | 1 | 0 | 3 |
Endosome | 0 | 0 | 1 | 0 | 1 | 2 | 3 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | – | – | – | 2 | 4 | 13 | 2 | 1 | 1 |
Golgi | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | – | – | – | 1 | 0 | 0 | 0 | 0 | 2 |
Mitochondria | 1 | 6 | 0 | 1 | 0 | 3 | 6 | 10 | 34 | 38 | 32 | 46 | 0 | 1 | 1 | – | – | – | 10 | 11 | 29 | 6 | 5 | 10 |
Nucleus | 0 | 1 | 0 | 0 | 3 | 1 | 4 | 5 | 2 | 3 | 3 | 2 | 0 | 2 | 1 | – | – | – | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 | 0 | 0 | 1 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | – | – | – | 1 | 0 | 1 | 0 | 0 | 1 |
SpindlePole | 2 | 0 | 11 | 8 | 6 | 8 | 6 | 8 | 4 | 10 | 9 | 17 | 4 | 7 | 6 | – | – | – | 0 | 1 | 2 | 2 | 1 | 0 |
Vac/Vac Membrane | 1 | 2 | 1 | 2 | 9 | 4 | 5 | 3 | 0 | 2 | 1 | 1 | 2 | 1 | 2 | – | – | – | 18 | 51 | 51 | 34 | 31 | 25 |
Unique Cell Count | 29 | 215 | 336 | 282 | 493 | 417 | 570 | 618 | 454 | 462 | 382 | 484 | 79 | 205 | 245 | 82 | 233 | 341 | 157 | 230 | 242 | |||
Labelled Cell Count | 33 | 229 | 364 | 310 | 520 | 437 | 603 | 656 | 495 | 504 | 429 | 536 | 84 | 229 | 263 | 82 | 233 | 341 | 157 | 230 | 242 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 4.7 | 3.3 | 5.2 | 5.1 | 5.6 | 4.8 | 4.7 | 4.8 | 4.1 | 4.1 | 4.1 | 3.9 | 5.1 | 5.2 | 4.9 | 3.5 | 4.5 | 4.5 | 5.9 | 5.8 | 6.1 |
Std Deviation (1e-4) | 1.1 | 0.6 | 1.4 | 2.0 | 1.8 | 1.6 | 1.6 | 1.5 | 1.6 | 1.2 | 1.6 | 0.7 | 1.1 | 1.4 | 1.4 | 0.9 | 1.7 | 1.1 | 1.7 | 1.9 | 2.6 |
Intensity Change (Log2) | – | – | – | -0.02 | 0.13 | -0.11 | -0.15 | -0.09 | -0.35 | -0.35 | -0.32 | -0.39 | -0.01 | 0.01 | -0.09 | -0.55 | -0.19 | -0.21 | 0.18 | 0.17 | 0.25 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |