Standard name
Human Ortholog
Description Protein that associates with mitochondrial ribosome; green fluorescent protein (GFP)-fusion protein localizes to the mitochondrion; expression may be cell cycle-regulated
DOWNLOAD

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0.06 0 0.05 0.06 0.05 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0.08 0.07 0 0 0 0 0 0
Cytoplasm 0.15 0.08 0.06 0.14 0.09 0.06 0 0 0 0 0 0 0.5 0.62 0.6 0.28 0.2 0.26 0 0 0 0 0 0
Endoplasmic Reticulum 0 0.07 0 0 0 0 0 0 0 0 0 0 0.05 0.09 0.05 0.08 0.05 0.07 0 0 0 0 0 0
Endosome 0.07 0 0.17 0 0.11 0 0 0.05 0 0 0 0 0.11 0.14 0.12 0.14 0.18 0.19 0.12 0.09 0.07 0.05 0.07 0.07
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.75 0.91 0.71 0.78 0.83 0.9 0.94 0.88 0.93 0.97 0.96 0.97 0.16 0 0.06 0.2 0.1 0.14 0.71 0.68 0.71 0.74 0.68 0.68
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0.28 0.14 0.09 0 0.05 0 0 0 0 0 0.05 0 0.07 0.06 0.2 0.16 0 0 0.08 0 0 0
SpindlePole 0.06 0 0.09 0.06 0.05 0 0.06 0.08 0 0 0 0 0.05 0.06 0 0.14 0.13 0.16 0 0 0 0 0 0
Vac/Vac Membrane 0.1 0.07 0.07 0 0.06 0.07 0 0.06 0.08 0.06 0.07 0.05 0.13 0.1 0.08 0.2 0.2 0.13 0 0.09 0 0.07 0.08 0.06
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 1 0 0 0 0 0 0 0 0 0 0 9 2 3 3 1 2 1 3 16 1 5 15
Bud 5 3 16 14 10 24 41 29 18 20 6 20 1 3 0 4 9 11 0 0 2 1 1 2
Bud Neck 1 0 1 0 0 0 0 0 0 1 0 0 2 2 1 0 0 2 1 0 1 2 1 7
Bud Site 2 0 0 0 0 8 20 9 7 7 0 3 0 0 0 0 1 1 – – – – – –
Cell Periphery 2 0 20 9 4 4 5 2 3 6 1 1 5 5 5 23 21 16 0 0 3 0 0 1
Cytoplasm 24 9 26 32 25 30 19 25 11 15 15 11 117 130 134 80 51 57 1 1 2 12 10 6
Endoplasmic Reticulum 0 7 2 1 2 1 0 2 2 9 8 21 12 19 12 24 12 16 3 0 2 3 1 2
Endosome 11 0 69 7 31 4 3 27 3 1 0 0 25 29 28 40 45 42 25 9 33 12 18 22
Golgi 0 0 1 0 0 0 0 0 1 0 0 0 0 0 0 3 2 4 2 0 14 1 6 6
Mitochondria 117 97 290 185 241 435 602 523 440 629 433 553 37 3 14 58 26 30 157 77 322 205 188 230
Nucleus 3 0 1 3 5 13 11 16 9 8 3 6 2 1 3 5 5 1 1 0 0 1 1 2
Nuclear Periphery 2 0 2 0 0 4 1 4 10 10 18 21 0 0 0 0 0 0 2 0 2 0 0 3
Nucleolus 4 0 0 0 1 5 12 6 3 7 5 2 0 0 0 0 1 1 0 0 1 2 1 2
Peroxisomes 4 0 114 33 27 17 29 18 8 1 1 1 12 0 16 18 50 34 6 3 35 3 3 2
SpindlePole 9 1 38 14 14 15 40 48 18 17 5 9 11 13 9 41 33 36 3 1 5 4 6 5
Vac/Vac Membrane 15 8 28 3 18 33 22 33 40 37 31 27 31 21 18 57 49 29 8 10 1 20 22 20
Unique Cell Count 155 107 410 237 292 482 640 597 471 650 452 572 235 210 224 287 250 219 222 113 452 279 278 338
Labelled Cell Count 199 126 608 301 378 593 805 742 573 768 526 675 264 228 243 356 306 282 222 113 452 279 278 338


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.6 4.2 4.6 4.3 4.3 3.9 3.8 4.1 3.7 3.6 3.5 3.5 5.7 5.7 5.8 6.5 6.1 5.8 5.7 6.1 6.4
Std Deviation (1e-4) 0.4 0.5 1.5 1.6 1.6 1.6 1.4 1.8 1.0 1.0 0.9 0.8 0.9 0.9 1.0 1.7 2.0 1.6 1.3 1.7 1.8
Intensity Change (Log2) – – – -0.09 -0.07 -0.23 -0.28 -0.17 -0.28 -0.34 -0.38 -0.38 0.33 0.32 0.34 0.52 0.43 0.34 0.34 0.43 0.49


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – 0 0 0
Bud Neck – – – – – – – – – – – – 0 0 0
Bud Site – – – – – – – – – – – – 0 0 0
Cell Periphery – – – – – – – – – – – – 0 0 0
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – 0 0 0
Endosome – – – – – – – – – – – – 0 0 0
Golgi – – – – – – – – – – – – 0 0 0
Mitochondria – – – – – – – – – – – – 0 0 0
Nuclear Periphery – – – – – – – – – – – – 0 0 0
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – 0 0 0
Peroxisomes – – – – – – – – – – – – 0 0 0
SpindlePole – – – – – – – – – – – – 0 0 0
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – 0 0 0
Cytoplasm – – – – – – – – – – – – 0 0 0
Nucleus – – – – – – – – – – – – 0 0 0
Vacuole – – – – – – – – – – – – 5.2 5.0 2.7