ORF
Human Ortholog
Description Protein of unknown function; may interact with ribosomes, based on co-purification experiments; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; protein abundance increases in response to DNA replication stress
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 – 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 – 0 0 0 0.05 0.11 0 0 0 0.05 0.07 0.13 0 0 0 0 0 0
Cytoplasm 0.97 0.98 0.96 0.99 0.94 0.9 – 0.71 0.74 0.78 0.71 0.65 0.98 0.99 0.99 0.98 0.96 0.96 0.95 0.96 0.91 0.89 0.9 0.92
Endoplasmic Reticulum 0 0 0 0 0 0 – 0.08 0.07 0.07 0.05 0.09 0 0 0 0 0.06 0.07 0 0 0 0 0.05 0
Endosome 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.06 – 0.12 0.17 0.13 0.23 0.27 0 0 0 0 0.05 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 – 0.09 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 1 0 7 – 6 4 1 5 19 0 0 0 0 0 0 0 1 0 0 0 0
Bud 0 0 9 0 0 0 – 4 2 1 2 2 3 0 0 0 0 0 0 0 0 1 1 1
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0
Bud Site 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 3 5 6 3 6 13 – 22 12 19 15 38 2 14 21 7 8 12 0 0 0 1 0 0
Cytoplasm 117 306 276 269 326 369 – 368 228 353 206 232 371 481 516 127 114 90 116 299 264 217 282 266
Endoplasmic Reticulum 0 2 1 1 10 10 – 43 20 30 14 33 8 5 3 4 7 7 0 1 9 7 14 8
Endosome 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 1 1 1 0 1 2 1 1
Golgi 0 0 0 0 0 3 – 4 0 1 2 4 0 0 0 0 0 0 0 2 0 1 3 1
Mitochondria 2 0 9 5 7 24 – 65 51 57 67 98 1 0 0 0 6 0 0 0 3 1 0 0
Nucleus 0 1 0 0 2 0 – 3 1 1 2 2 0 0 0 0 0 0 0 0 1 0 0 1
Nuclear Periphery 0 1 0 1 0 0 – 2 1 4 0 0 0 0 0 0 0 0 0 0 1 2 0 0
Nucleolus 0 0 0 0 0 0 – 1 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 1 0 3 0 1 4 – 47 17 17 9 9 2 2 1 1 4 2 1 3 2 4 4 3
Unique Cell Count 120 311 287 271 346 412 521 307 451 290 359 380 488 520 129 119 94 122 313 291 244 315 288
Labelled Cell Count 123 315 304 280 352 430 565 336 484 322 437 387 502 542 139 140 112 122 313 291 244 315 288


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 17.8 20.0 11.4 16.1 16.0 15.1 – 14.2 14.4 13.9 13.9 14.2 20.5 21.6 21.2 23.3 22.4 21.5 20.5 21.2 20.6
Std Deviation (1e-4) 3.9 3.6 2.7 2.9 2.9 3.0 – 3.1 3.2 3.4 3.6 3.7 4.0 3.8 3.9 6.4 7.5 6.0 5.6 6.8 4.6
Intensity Change (Log2) – – – 0.5 0.49 0.41 – 0.32 0.34 0.29 0.29 0.32 0.85 0.93 0.9 1.03 0.98 0.92 0.85 0.9 0.86


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 – 0 0 0 0 4.0 0 0 0 0 0 0
Bud 0 0 0 – 0 -2.2 0 -2.1 0 -2.3 0 0 0 0 0
Bud Neck 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 -0.3 0.9 – 1.6 1.3 1.6 2.0 4.3 0 0.7 1.5 0 0 0
Cytoplasm 2.4 -1.1 -3.2 – -8.6 -7.4 -6.7 -8.1 -9.7 1.1 2.1 3.1 1.2 -0.2 -0.2
Endoplasmic Reticulum 0 0 0 – 4.7 4.1 4.2 3.4 5.0 0 0 0 0 0 0
Endosome 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Mitochondria -1.0 -0.9 1.6 – 4.4 5.4 4.4 7.1 8.2 0 0 0 0 0 0
Nucleus 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 – 4.5 3.0 2.2 1.7 1.4 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 20.5405 28.6307 24.9854 22.4802 23.1822 24.7646 15.7791 22.9221 20.3277 19.427 13.7014 20.5599 23.8595 31.1301 28.8008 26.8681 23.928 27.1981
Actin 0.0057 0.0003 0.0029 0.0235 0.0002 0.0004 0.0061 0.0001 0.003 0.0014 0.0161 0.0007 0.0038 0.0002 0.0023 0.0055 0.001 0.0008
Bud 0.0004 0.0001 0.0003 0.0016 0.0002 0.0002 0.0019 0.0001 0.0005 0.0005 0.0013 0.0004 0.0002 0.0001 0.0002 0.0003 0.0016 0.0001
Bud Neck 0.0004 0.0002 0.0004 0.002 0.0005 0.0006 0.0007 0.0001 0.0005 0.0004 0.0007 0.0006 0.0005 0.0001 0.0002 0.0006 0.0007 0.0009
Bud Periphery 0.0003 0 0.0002 0.001 0.0002 0.0001 0.0009 0 0.0004 0.0005 0.0018 0.0002 0.0001 0 0.0001 0.0001 0.001 0
Bud Site 0.0008 0.0003 0.0004 0.0163 0.0002 0 0.0011 0.0001 0.001 0.0004 0.0007 0 0.0005 0 0.0002 0.0001 0.0007 0
Cell Periphery 0.0002 0.0001 0.0001 0.0003 0.0001 0 0.0002 0 0.0002 0.0001 0.0003 0 0.0002 0 0 0 0.0001 0
Cytoplasm 0.8712 0.994 0.9345 0.8344 0.8547 0.9753 0.8164 0.9926 0.9157 0.8836 0.6763 0.9543 0.9225 0.9885 0.9695 0.9522 0.887 0.9686
Cytoplasmic Foci 0.0247 0.001 0.0105 0.0282 0.0088 0.0026 0.0202 0.0013 0.0106 0.0069 0.0197 0.0074 0.0071 0.0013 0.0031 0.0025 0.0126 0.0024
Eisosomes 0.0001 0 0 0.0001 0 0 0.0002 0 0 0 0.0004 0 0.0001 0 0 0 0 0
Endoplasmic Reticulum 0.0092 0.0009 0.0043 0.0038 0.0029 0.0081 0.0116 0.0011 0.0032 0.0107 0.004 0.0118 0.0235 0.0035 0.0049 0.0022 0.0047 0.0065
Endosome 0.0246 0.0006 0.0153 0.0361 0.0389 0.0053 0.0476 0.0014 0.0246 0.0493 0.079 0.0154 0.0123 0.0008 0.0068 0.0062 0.0159 0.0085
Golgi 0.0042 0.0001 0.005 0.0127 0.0031 0.0004 0.0108 0.0001 0.0033 0.0039 0.0518 0.0018 0.0012 0.0001 0.0007 0.001 0.0015 0.0006
Lipid Particles 0.0115 0.0002 0.0052 0.0101 0.0317 0 0.0095 0 0.0047 0.0012 0.0174 0 0.0022 0 0.0012 0.0014 0.0015 0.0003
Mitochondria 0.0039 0 0.0031 0.0025 0.0081 0.0005 0.0225 0.0001 0.0067 0.0053 0.0838 0.0015 0.0013 0 0.0004 0.0004 0.0059 0.0002
None 0.0046 0.0002 0.0007 0.0014 0.003 0.0002 0.0103 0.0002 0.0005 0.0024 0.001 0.0003 0.0024 0.0001 0.0005 0.0007 0.0054 0.0005
Nuclear Periphery 0.0075 0.0004 0.0021 0.0027 0.0039 0.0014 0.0056 0.0006 0.004 0.0086 0.0056 0.0012 0.0082 0.0014 0.002 0.0098 0.0114 0.0039
Nucleolus 0.0011 0 0.0001 0.0004 0.0007 0 0.0003 0 0.0008 0.0001 0.0015 0 0.0003 0 0.0001 0.0003 0.0033 0.0001
Nucleus 0.0063 0.0007 0.0018 0.0024 0.0025 0.0016 0.0095 0.0006 0.0042 0.0022 0.003 0.001 0.0046 0.003 0.0013 0.0022 0.0224 0.0024
Peroxisomes 0.0051 0 0.0048 0.0041 0.0067 0 0.0099 0 0.0056 0.0001 0.0015 0.0001 0.0004 0 0.0023 0.0007 0.0015 0
Punctate Nuclear 0.0081 0.0001 0.0005 0.0027 0.0004 0.0001 0.0011 0 0.0003 0.0003 0.0003 0.0001 0.0015 0 0.0014 0.0076 0.0148 0.0016
Vacuole 0.0081 0.0006 0.0062 0.0111 0.0199 0.0024 0.0102 0.0012 0.0076 0.0157 0.0155 0.0023 0.0057 0.0007 0.0022 0.0047 0.0052 0.0017
Vacuole Periphery 0.0021 0.0001 0.0015 0.0026 0.0131 0.0008 0.0033 0.0003 0.0026 0.0063 0.0184 0.0009 0.0014 0.0001 0.0007 0.0016 0.0018 0.0007

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 162.6333 172.2244 180.9088 176.4579 196.2631 167.5446 136.8211 173.2423 190.0247 194.3444
Translational Efficiency 3.1664 2.8329 2.4346 3.2498 2.4724 3.0486 3.092 2.3689 2.3847 2.6346

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
2573 1213 2747 1404 951 1894 2035 1527 3524 3107 4782 2931

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 1301.76 1486.93 1806.58 2235.82 1105.28 1500.76 2005.92 2099.92 1248.74 1495.36 1891.41 2165.02
Standard Deviation 197.32 271.31 327.08 443.71 214.20 272.32 348.36 419.41 220.04 272.01 350.44 436.53
Intensity Change Log 2 — 0.191873 0.472798 0.780341 — 0.441281 0.859852 0.925922 — 0.311770 0.663477 0.849018

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000083 0.000374 0.000057 0.000324 0.000300 0.000484 0.000068 0.000540 0.000142 0.000441 0.000062 0.000437
Bud Neck 0.000260 0.000313 0.000510 0.002190 0.000425 0.000261 0.000783 0.001126 0.000304 0.000281 0.000626 0.001636
Bud Site 0.006647 0.011392 0.004666 0.015636 0.049568 0.011318 0.006143 0.015286 0.018230 0.011347 0.005294 0.015454
Cell Periphery 0.000079 0.000144 0.000096 0.001516 0.000601 0.000102 0.000216 0.001189 0.000220 0.000119 0.000147 0.001346
Cytoplasm 0.919012* 0.910450* 0.912227* 0.807056* 0.575415* 0.896535* 0.868754* 0.854442* 0.826287* 0.901968* 0.893727* 0.831743*
Cytoplasmic Foci 0.044506 0.034634 0.015050 0.014007 0.218269* 0.048727 0.016981 0.016916 0.091398 0.043225 0.015872 0.015522
Eisosomes 0.000002 0.000002 0.000001 0.000003 0.000005 0.000002 0.000001 0.000004 0.000003 0.000002 0.000001 0.000003
Endoplasmic Reticulum 0.000430 0.000905 0.000160 0.000514 0.004711 0.000705 0.000661 0.000280 0.001585 0.000783 0.000373 0.000392
Endosome 0.001561 0.002943 0.001818 0.004155 0.009539 0.003226 0.002660 0.003629 0.003714 0.003116 0.002176 0.003881
Golgi 0.007435 0.012885 0.004590 0.017691 0.039942 0.013091 0.008520 0.015603 0.016207 0.013010 0.006262 0.016603
Lipid Particles 0.000411 0.000677 0.000898 0.006703 0.000575 0.000609 0.002078 0.005347 0.000455 0.000636 0.001400 0.005997
Mitochondria 0.000374 0.000655 0.000106 0.000758 0.001744 0.001306 0.000102 0.001512 0.000744 0.001052 0.000105 0.001151
Mitotic Spindle 0.000531 0.003769 0.002794 0.007036 0.004650 0.002584 0.004772 0.003443 0.001643 0.003046 0.003635 0.005164
None 0.001457 0.000816 0.000560 0.000419 0.001223 0.001061 0.000388 0.000458 0.001394 0.000965 0.000487 0.000439
Nuclear Periphery 0.000010 0.000051 0.000060 0.000327 0.000098 0.000020 0.000112 0.000051 0.000034 0.000032 0.000082 0.000183
Nuclear Periphery Foci 0.000336 0.001203 0.001337 0.005248 0.001158 0.000928 0.003350 0.002251 0.000558 0.001036 0.002193 0.003687
Nucleolus 0.000067 0.000061 0.000102 0.000223 0.000215 0.000064 0.000097 0.000122 0.000107 0.000063 0.000100 0.000170
Nucleus 0.004029 0.003708 0.009145 0.009724 0.003313 0.002397 0.007714 0.006699 0.003836 0.002909 0.008536 0.008148
Peroxisomes 0.000192 0.000205 0.000101 0.000261 0.000295 0.000180 0.000095 0.000335 0.000220 0.000190 0.000098 0.000300
Vacuole 0.008813 0.008402 0.039007 0.091300 0.018445 0.010279 0.066363 0.062932 0.011413 0.009546 0.050649 0.076520
Vacuole Periphery 0.003764 0.006412 0.006715 0.014908 0.069511 0.006120 0.010143 0.007834 0.021507 0.006234 0.008174 0.011223

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -2.07 1.40 -4.98 0.29 -5.48 -0.67 5.03 -1.43 -0.17 -2.86 -1.73 4.55 -3.41 0.01 -4.29
Bud Neck -1.27 -7.58 -8.89 -8.59 -7.40 3.37 -7.26 -8.72 -10.38 -3.81 0.66 -11.30 -12.07 -12.14 -8.55
Bud Site -4.11 3.35 -7.02 -2.54 -8.46 15.02* 18.28* 13.07 -2.92 -7.47 6.26 15.57 2.15 -3.84 -11.48
Cell Periphery -4.00 -1.52 -9.23 -8.75 -9.10 6.74 4.96 -3.68 -8.53 -7.43 4.50 2.91 -10.90 -12.26 -11.87
Cytoplasm 1.56 3.66 17.70 14.06 15.54 -29.50 -26.07 -23.40 7.38 2.13 -14.15 -12.16 1.20 15.07 13.18
Cytoplasmic Foci 3.64 15.68 16.16 9.26 1.34 24.39 30.23 30.16 12.93 0.58 16.12 28.99 28.92 16.12 1.01
Eisosomes -0.99 6.56 -4.45 -2.17 -8.48 3.59 10.02 1.54 -1.65 -4.77 0.68 11.37 -2.38 -2.30 -7.81
Endoplasmic Reticulum -1.44 3.29 -0.29 1.36 -4.63 5.28 5.09 5.97 2.96 1.74 3.06 5.00 5.70 2.73 0.36
Endosome -3.41 -1.90 -4.55 -1.37 -3.83 11.12 13.67 9.25 -0.31 -1.17 1.93 6.90 0.33 -1.11 -3.68
Golgi -3.72 5.53 -5.53 -1.29 -7.71 14.44 18.74 11.77 -0.58 -3.64 3.27 15.46 1.26 -1.34 -8.11
Lipid Particles -2.58 -6.97 -9.91 -9.46 -9.03 -0.18 -7.57 -10.01 -10.05 -6.32 -1.97 -10.91 -14.17 -13.57 -11.36
Mitochondria -0.97 2.54 -1.01 0.07 -2.36 0.82 8.23 0.72 -0.02 -2.21 -0.91 6.72 -0.75 0.14 -2.88
Mitotic Spindle -4.13 -7.13 -5.21 -1.88 -2.77 2.19 -0.56 1.29 -0.65 1.83 -2.68 -5.29 -4.22 -2.01 -0.82
None 4.95 8.47 9.22 4.19 2.25 0.36 2.47 2.28 1.56 -1.16 1.63 7.55 7.71 2.14 0.96
Nuclear Periphery -1.77 -13.10 -3.92 -3.24 -3.11 2.37 -0.75 1.21 -5.12 2.85 0.15 -4.44 -3.82 -3.86 -2.14
Nuclear Periphery Foci -5.31 -8.97 -9.55 -7.54 -7.27 0.45 -4.56 -2.84 -3.94 2.44 -2.45 -8.95 -10.80 -8.23 -4.54
Nucleolus 0.20 -4.39 -3.33 -3.24 -2.57 1.22 0.95 0.68 -2.66 -3.04 1.13 0.05 -1.87 -4.01 -3.14
Nucleus 0.84 -14.66 -9.43 -9.41 -2.84 1.01 -5.56 -5.15 -12.00 0.67 2.97 -14.33 -11.82 -14.36 -1.73
Peroxisomes -0.33 4.35 -0.85 -0.61 -1.93 3.77 7.15 -0.57 -3.25 -5.37 1.23 7.33 -1.60 -2.13 -4.27
Vacuole 0.34 -18.26 -22.87 -22.73 -13.51 4.31 -15.97 -14.72 -17.87 -0.07 1.94 -25.25 -28.16 -28.53 -10.33
Vacuole Periphery -2.23 -4.16 -7.22 -3.93 -4.89 15.07 14.27 14.79 -0.95 2.43 10.95 10.40 8.11 -3.87 -2.52
ORF
Human Ortholog
Description Protein of unknown function; may interact with ribosomes, based on co-purification experiments; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; protein abundance increases in response to DNA replication stress
Localization
Cell Percentages cytoplasm (99%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-5

YPL225W

YPL225W


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ypl225w-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available