ORF
Human Ortholog
Description Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; not an essential gene; YPL229W has a paralog, YMR181C, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.09 0 0 0 0 0 0 0
Cytoplasm 0.98 0.96 0.97 0.99 0.94 0.82 0.72 0.71 0.65 0.63 0.6 0.6 1.0 0.99 0.99 0.97 1.0 0.99 0.95 0.93 0.92 0.87 0.86 0.85
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0.07 0.14 0.38 0.47 0.38 0.54 0.47 0.56 0.55 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0.07 0.07 0.07 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 1 0 2
Bud 0 2 2 2 4 7 13 13 16 16 24 14 0 0 0 0 0 0 0 1 0 1 3 5
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 1
Bud Site 0 0 0 0 0 0 2 1 1 2 4 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 1 2 2 4 7 4 9 5 12 23 10 14 1 1 10 7 13 5 0 0 0 0 0 0
Cytoplasm 214 183 260 316 519 590 602 680 448 549 418 369 243 378 454 145 151 142 222 179 246 252 247 313
Endoplasmic Reticulum 1 0 3 0 2 4 0 4 2 6 6 14 0 2 2 6 4 3 1 0 2 7 5 7
Endosome 1 0 1 2 6 4 5 8 5 5 1 2 0 1 0 0 0 0 1 2 2 1 3 1
Golgi 0 1 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 1 1 2 3
Mitochondria 1 4 11 22 75 277 396 371 371 410 386 337 0 0 0 3 0 1 2 3 5 6 10 11
Nucleus 0 0 0 0 0 2 1 11 3 7 1 7 1 0 1 0 0 2 0 0 1 0 0 1
Nuclear Periphery 0 0 0 0 0 3 2 8 0 5 3 1 0 0 0 0 0 0 0 0 1 1 0 0
Nucleolus 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0
Vac/Vac Membrane 1 0 0 3 3 11 12 64 50 60 26 13 0 0 1 0 0 1 1 0 0 7 7 9
Unique Cell Count 218 190 267 320 551 721 836 964 684 878 692 612 243 380 457 149 151 144 233 194 267 290 288 369
Labelled Cell Count 219 192 280 349 617 902 1042 1167 908 1083 879 771 245 383 468 161 168 154 233 194 267 290 288 369


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.6 6.1 5.0 4.9 4.8 4.5 4.0 4.5 4.1 4.2 4.3 4.2 6.8 6.7 6.8 6.5 6.8 6.8 7.6 7.3 7.7
Std Deviation (1e-4) 0.6 0.8 0.8 1.2 1.2 1.1 0.9 1.1 0.9 0.8 1.2 1.1 1.2 1.2 1.0 1.0 1.1 1.0 1.6 1.5 1.6
Intensity Change (Log2) – – – -0.02 -0.06 -0.15 -0.33 -0.16 -0.28 -0.24 -0.21 -0.26 0.45 0.42 0.46 0.39 0.44 0.44 0.6 0.56 0.62


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 4.2 0
Cytoplasm 1.2 -2.0 -6.3 -8.7 -9.1 -10.1 -11.0 -11.3 -11.2 2.5 2.2 2.2 0 2.0 0.8
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 1.4 0 0 0 0 0 0 0 0 -3.2 0 0 -1.1 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 4.3 4.5 4.4 3.2 0 0 0 0 0 0 0