Standard name | |
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Human Ortholog | |
Description | Ferredoxin of the mitochondrial matrix; required for formation of cellular iron-sulfur proteins; involved in heme A biosynthesis; human homolog FDX1L can complement yeast by allowing growth during down-regulation of yeast YAH1 |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | – | 0 | 0 | 2 | 0 | 0 | 1 | 4 | 4 |
Bud | 0 | 0 | – | 0 | 0 | 0 | 0 | 3 | 3 | 1 | – | 2 | 0 | 0 | 1 | – | 0 | 0 | 0 | 0 | 0 | 1 | 5 | 19 |
Bud Neck | 0 | 0 | – | 0 | 1 | 1 | 8 | 5 | 2 | 4 | – | 0 | 0 | 3 | 0 | – | 2 | 0 | 0 | 0 | 0 | 0 | 1 | 1 |
Bud Site | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | – | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 1 | – | 1 | 0 | 1 | 4 | 3 | 0 | 0 | – | 0 | 0 | 3 | 0 | – | 5 | 8 | 0 | 0 | 0 | 4 | 4 | 4 |
Cytoplasm | 220 | 127 | – | 157 | 205 | 106 | 248 | 240 | 66 | 134 | – | 9 | 279 | 793 | 778 | – | 315 | 351 | 200 | 120 | 19 | 325 | 581 | 591 |
Endoplasmic Reticulum | 1 | 0 | – | 1 | 0 | 0 | 2 | 0 | 0 | 0 | – | 0 | 0 | 1 | 1 | – | 17 | 19 | 0 | 0 | 0 | 1 | 2 | 2 |
Endosome | 0 | 0 | – | 0 | 0 | 0 | 1 | 1 | 0 | 0 | – | 0 | 0 | 0 | 0 | – | 1 | 0 | 3 | 0 | 0 | 1 | 2 | 2 |
Golgi | 0 | 0 | – | 1 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | – | 0 | 1 | 0 | 0 | 0 | 2 | 0 | 0 |
Mitochondria | 7 | 6 | – | 1 | 2 | 12 | 11 | 30 | 41 | 46 | – | 11 | 1 | 0 | 1 | – | 8 | 8 | 25 | 9 | 2 | 23 | 25 | 25 |
Nucleus | 0 | 0 | – | 0 | 1 | 1 | 3 | 1 | 1 | 1 | – | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | – | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 |
Nucleolus | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 2 | 1 | – | 2 | 2 | 0 | 6 | 3 | 0 | 1 | – | 1 | 0 | 2 | 0 | – | 0 | 1 | 3 | 0 | 0 | 5 | 8 | 13 |
Vac/Vac Membrane | 0 | 0 | – | 0 | 0 | 0 | 6 | 1 | 0 | 0 | – | 0 | 0 | 1 | 0 | – | 5 | 4 | 2 | 2 | 0 | 2 | 4 | 4 |
Unique Cell Count | 227 | 130 | 160 | 208 | 110 | 269 | 262 | 94 | 160 | 17 | 279 | 797 | 780 | 323 | 357 | 243 | 136 | 25 | 376 | 651 | 686 | |||
Labelled Cell Count | 230 | 135 | 163 | 211 | 121 | 289 | 287 | 113 | 187 | 23 | 280 | 803 | 781 | 353 | 393 | 243 | 136 | 25 | 376 | 651 | 686 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 3.7 | 3.9 | – | 4.8 | 5.3 | 3.7 | 4.3 | 4.2 | 3.4 | 3.7 | – | 4.0 | 4.5 | 4.2 | 4.8 | 4.6 | 4.9 | 5.3 | 4.1 | 3.9 | 4.1 |
Std Deviation (1e-4) | 0.7 | 0.5 | – | 1.6 | 1.5 | 1.3 | 1.2 | 1.2 | 1.3 | 1.2 | – | 2.0 | 0.9 | 1.0 | 0.9 | 1.0 | 0.9 | 1.3 | 1.4 | 1.0 | 1.4 |
Intensity Change (Log2) | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | -0.9181 | -0.3202 | -0.5187 | -0.7303 | -0.8445 | -0.439 | 0.5718 | 0.6372 | 0.3478 | 0.0176 | 0.4739 | 0.5537 | 1.3025 | 1.8372 | 1.7608 | 1.9204 | 1.3927 | 1.6033 |
Actin | 0.0041 | 0.0004 | 0.0002 | 0.0003 | 0.001 | 0.0003 | 0.0312 | 0.0002 | 0.0056 | 0.007 | 0.0243 | 0.0006 | 0.0096 | 0.0001 | 0.0211 | 0.0001 | 0.0383 | 0.0064 |
Bud | 0.0001 | 0.0052 | 0.0002 | 0.0013 | 0.001 | 0.0003 | 0.001 | 0.0001 | 0.0025 | 0.0002 | 0.0002 | 0.0002 | 0.0006 | 0.0002 | 0.0009 | 0.0002 | 0.0183 | 0.0004 |
Bud Neck | 0.0001 | 0.0003 | 0.0009 | 0.0002 | 0.0023 | 0.0004 | 0.0066 | 0 | 0.0002 | 0.0005 | 0.0004 | 0.0004 | 0.0003 | 0 | 0.0009 | 0.0004 | 0.0003 | 0.001 |
Bud Periphery | 0.0002 | 0.0027 | 0.0002 | 0.0013 | 0.0008 | 0.0002 | 0.0022 | 0 | 0.0043 | 0.0001 | 0.0004 | 0.0003 | 0.0009 | 0.0002 | 0.0015 | 0.0002 | 0.0178 | 0.0005 |
Bud Site | 0.0001 | 0.0192 | 0.0013 | 0.0003 | 0.0023 | 0.0001 | 0.0084 | 0.0003 | 0.0019 | 0.0003 | 0.0006 | 0.0003 | 0.0016 | 0.0002 | 0.0118 | 0.0032 | 0.0015 | 0.0001 |
Cell Periphery | 0 | 0.0003 | 0.0001 | 0.0002 | 0.0001 | 0 | 0.0005 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0003 | 0 | 0.0002 | 0 |
Cytoplasm | 0.058 | 0.0716 | 0.0956 | 0.0999 | 0.082 | 0.1102 | 0.1125 | 0.2672 | 0.1808 | 0.2843 | 0.2439 | 0.324 | 0.0728 | 0.1415 | 0.1704 | 0.1222 | 0.1371 | 0.1957 |
Cytoplasmic Foci | 0.0065 | 0.0092 | 0.0097 | 0.0265 | 0.0091 | 0.0082 | 0.0378 | 0.0073 | 0.0128 | 0.034 | 0.0129 | 0.0097 | 0.0058 | 0.0017 | 0.0129 | 0.036 | 0.0135 | 0.0124 |
Eisosomes | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0 | 0.001 | 0.0002 | 0.0002 | 0.0003 | 0.0005 | 0.0001 | 0.0002 | 0.0001 | 0.0002 | 0.0001 | 0.0002 | 0.0001 |
Endoplasmic Reticulum | 0.0007 | 0.0009 | 0.0009 | 0.0006 | 0.0006 | 0.0004 | 0.0049 | 0.0014 | 0.0035 | 0.0012 | 0.0013 | 0.001 | 0.0032 | 0.0005 | 0.0011 | 0.0004 | 0.0013 | 0.0009 |
Endosome | 0.0012 | 0.001 | 0.0025 | 0.001 | 0.0018 | 0.0008 | 0.0255 | 0.0012 | 0.007 | 0.0141 | 0.0057 | 0.0024 | 0.0058 | 0.0002 | 0.0031 | 0.0036 | 0.0009 | 0.0016 |
Golgi | 0.0005 | 0.0001 | 0.0002 | 0.0001 | 0.0003 | 0.0001 | 0.0084 | 0.0001 | 0.0018 | 0.0022 | 0.005 | 0.0004 | 0.001 | 0 | 0.0017 | 0.0026 | 0.0008 | 0.0015 |
Lipid Particles | 0.0005 | 0.0005 | 0.0003 | 0.0018 | 0.0008 | 0.0002 | 0.0223 | 0.0003 | 0.0051 | 0.02 | 0.0273 | 0.0017 | 0.0022 | 0.0001 | 0.0037 | 0.0007 | 0.0007 | 0.0036 |
Mitochondria | 0.0004 | 0.0003 | 0.0006 | 0.0007 | 0.0004 | 0.0002 | 0.0123 | 0.0002 | 0.0101 | 0.0008 | 0.0348 | 0.0055 | 0.0007 | 0.0001 | 0.0086 | 0.0003 | 0.0006 | 0.0005 |
None | 0.9235 | 0.8795 | 0.8748 | 0.859 | 0.869 | 0.8669 | 0.6555 | 0.7193 | 0.7477 | 0.6172 | 0.5934 | 0.6467 | 0.8784 | 0.8541 | 0.7495 | 0.8228 | 0.7608 | 0.7708 |
Nuclear Periphery | 0.0009 | 0.0005 | 0.0009 | 0.0003 | 0.0008 | 0.0005 | 0.0209 | 0.0004 | 0.0021 | 0.0011 | 0.0025 | 0.0024 | 0.0046 | 0.0002 | 0.0007 | 0.0001 | 0.0007 | 0.0003 |
Nucleolus | 0.0002 | 0.0007 | 0.0006 | 0.001 | 0.0031 | 0.0003 | 0.0016 | 0.0001 | 0.0003 | 0.0009 | 0.0011 | 0.0002 | 0.0003 | 0 | 0.0004 | 0.0001 | 0.0003 | 0 |
Nucleus | 0.0006 | 0.0028 | 0.0027 | 0.0007 | 0.004 | 0.003 | 0.005 | 0.0006 | 0.0012 | 0.0018 | 0.0044 | 0.001 | 0.0026 | 0.0003 | 0.0007 | 0.0004 | 0.0014 | 0.0005 |
Peroxisomes | 0.0002 | 0.0008 | 0.0003 | 0.0007 | 0.0006 | 0.0005 | 0.0179 | 0.0001 | 0.0084 | 0.0048 | 0.0362 | 0.0004 | 0.0014 | 0 | 0.005 | 0.0056 | 0.001 | 0.0026 |
Punctate Nuclear | 0.0015 | 0.0031 | 0.0068 | 0.0029 | 0.0194 | 0.007 | 0.0177 | 0.0004 | 0.0018 | 0.0064 | 0.0038 | 0.0017 | 0.0059 | 0.0002 | 0.0045 | 0.0006 | 0.0035 | 0.0007 |
Vacuole | 0.0003 | 0.0006 | 0.001 | 0.001 | 0.0005 | 0.0003 | 0.0047 | 0.0005 | 0.002 | 0.0022 | 0.0009 | 0.0006 | 0.0013 | 0.0002 | 0.0009 | 0.0002 | 0.0005 | 0.0002 |
Vacuole Periphery | 0.0001 | 0.0001 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0.0021 | 0.0001 | 0.0005 | 0.0005 | 0.0005 | 0.0002 | 0.0004 | 0 | 0.0001 | 0 | 0.0001 | 0.0001 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 73.3477 | 53.2149 | 53.5905 | 43.4534 | 62.0208 | 48.8714 | 61.0116 | 53.8242 | 53.145 | 63.8619 |
Translational Efficiency | 1.1495 | 1.4532 | 1.3093 | 1.932 | 1.3992 | 1.7947 | 1.2139 | 1.7069 | 1.5544 | 1.4148 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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