Standard name
Human Ortholog
Description Spindle positioning factor; orients astral microtubules, connecting them to actin cables at the cortex with Bim1p and Myo2, resulting in proper spindle positioning; targeted for StuBL-dependent degradation at kinetochores by Slx5p-Slx8p, ensuring chromosome transmission fidelity and correct spindle positioning; role in karyogamy; localizes to the shmoo tip, the growing bud-tip, the nucleus, the kinetochore, the spindle and microtubules; homolog of adenomatous polyposis coli
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0.07 0.11 0.05 0 0 0 0 0 0 0 0.08 0.09 0 0 0 0 0.06 0.06 0.05 0.11 0.12 0.14
Bud 0.08 0.06 0.06 0.11 0.09 0.05 0.08 0.14 0.07 0.16 0.1 0.05 0.05 0.06 0 0 0.05 0 0 0 0 0.09 0.06 0.07
Bud Neck 0 0 0.21 0.13 0.14 0.1 0.09 0.17 0.08 0.09 0.05 0 0.1 0.11 0.07 0 0 0 0.05 0.05 0.08 0.1 0.07 0.07
Bud Site 0 0 0 0 0 0 0.06 0 0 0 0.05 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.4 0.39 0.43 0.43 0.39 0.4 0.31 0.34 0.25 0.29 0.29 0.34 0.54 0.41 0.44 0.41 0.35 0.39 0.19 0.16 0.18 0.24 0.21 0.2
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0 0 0 0 0 0 0 0
Endosome 0.05 0.15 0 0 0 0 0.05 0 0 0 0 0 0 0.12 0.12 0.12 0.2 0.22 0.12 0.14 0.1 0.08 0.07 0.06
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.16 0.05 0 0.21 0 0.18 0.16 0.17 0.33 0.41 0.56 0.4 0 0 0 0.07 0 0 0.09 0.12 0.17 0.08 0.08 0.1
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0.06 0.08 0 0 0 0.05 0.05 0 0 0 0 0 0 0 0.06 0 0.05 0.11 0.07 0 0 0.05 0 0
SpindlePole 0.38 0.36 0.38 0.26 0.47 0.38 0.45 0.48 0.4 0.38 0.24 0.31 0.34 0.44 0.44 0.41 0.46 0.44 0.38 0.33 0.26 0.18 0.31 0.26
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 6 9 6 6 6 3 4 6 1 0 0 2 10 13 3 6 8 2 13 24 6 16 22 30
Bud 16 20 5 6 10 8 17 35 10 28 20 10 6 9 7 6 10 5 5 13 5 14 11 16
Bud Neck 5 12 19 7 16 14 19 42 12 17 10 7 13 16 13 5 9 6 12 21 10 15 12 14
Bud Site 8 8 0 1 2 4 12 9 4 3 10 6 2 2 0 8 2 4 – – – – – –
Cell Periphery 0 1 0 0 0 0 0 0 0 1 0 2 0 0 0 1 2 2 0 1 0 0 0 2
Cytoplasm 77 126 39 23 45 59 68 86 38 53 57 67 70 61 82 90 75 79 44 64 24 35 40 44
Endoplasmic Reticulum 1 4 2 2 0 2 0 3 1 1 1 4 0 0 0 15 6 8 0 0 2 0 0 0
Endosome 10 49 0 0 5 5 11 4 6 3 2 6 4 17 23 27 42 44 26 57 13 12 13 12
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 3 0 2 0 0 0 0
Mitochondria 32 16 2 11 2 26 34 42 50 74 111 78 1 1 0 15 3 8 20 46 23 12 15 22
Nucleus 0 0 2 1 1 3 0 0 0 1 2 1 0 0 0 3 3 2 0 1 0 1 0 0
Nuclear Periphery 1 0 0 0 0 0 0 0 1 0 0 1 0 0 0 0 0 1 0 0 0 0 0 0
Nucleolus 0 1 0 0 0 0 1 1 0 3 2 1 0 0 0 1 1 3 0 1 0 0 0 0
Peroxisomes 12 27 0 1 1 7 10 2 5 4 1 7 2 6 11 9 10 23 14 17 5 7 4 9
SpindlePole 73 115 34 14 55 56 98 119 61 69 47 62 44 64 83 90 99 89 86 134 36 27 58 56
Vac/Vac Membrane 0 8 1 0 2 1 6 0 2 3 1 3 0 0 0 8 6 3 1 7 1 1 2 1
Unique Cell Count 194 323 90 53 116 146 216 250 151 180 199 197 129 147 188 222 213 202 228 402 137 149 189 219
Labelled Cell Count 241 396 110 72 145 188 280 349 191 260 264 257 152 189 222 285 276 282 228 402 137 149 189 219


Ambiguous, SpindlePole

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.7 4.6 5.4 4.5 5.4 4.2 4.3 4.3 3.7 3.8 3.3 4.8 5.3 5.2 5.0 6.4 6.1 5.8 4.7 5.1 5.2
Std Deviation (1e-4) 0.6 0.8 2.1 1.0 1.7 1.2 1.3 0.9 1.0 1.5 1.6 4.1 1.0 0.9 1.3 2.0 1.5 1.5 1.1 1.3 1.2
Intensity Change (Log2) – – – -0.26 0.01 -0.35 -0.33 -0.32 -0.55 -0.52 -0.72 -0.18 -0.04 -0.05 -0.1 0.25 0.18 0.09 -0.22 -0.08 -0.05


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -0.5444 -1.0464 -0.9002 -1.2633 -1.3374 -0.8629 -0.9323 -0.4936 -0.4676 -0.8351 -0.8123 -0.3997 -1.4092 -0.7453 -0.8649 -0.742 -0.7751 -0.8356
Actin 0.0436 0.016 0.0094 0.0024 0.0345 0.0062 0.0033 0.0047 0.0055 0.0006 0.0018 0.0056 0.0012 0.0028 0.0017 0.0016 0.0015 0.0024
Bud 0.0011 0.0007 0.0049 0.0007 0.0073 0.0041 0.0005 0.0009 0.0004 0.0001 0.0013 0.0002 0.0013 0.0034 0.0091 0.0001 0.0013 0.0002
Bud Neck 0.0035 0.0022 0.0699 0.0534 0.0006 0.0236 0.0133 0.0071 0.1024 0.108 0.0006 0.0128 0.0075 0.01 0.0586 0.0076 0.0007 0.0279
Bud Periphery 0.002 0.0013 0.003 0.0025 0.0173 0.0022 0.0009 0.0014 0.0007 0.0003 0.0061 0.0004 0.0009 0.0017 0.0058 0.0001 0.0073 0.0003
Bud Site 0.0049 0.025 0.008 0.0039 0.0019 0.0011 0.0151 0.0517 0.0139 0.0012 0.0012 0.0006 0.0048 0.0658 0.0242 0.0007 0.0013 0.0007
Cell Periphery 0.0005 0.0004 0.0005 0.0002 0.0003 0.0002 0.0003 0.0004 0.0002 0.0001 0.0001 0.0001 0.0002 0.0008 0.0002 0.0001 0.0003 0.0002
Cytoplasm 0.0413 0.045 0.0177 0.0094 0.014 0.0675 0.021 0.0313 0.0096 0.0084 0.0065 0.0276 0.0369 0.0756 0.0287 0.0246 0.0674 0.0682
Cytoplasmic Foci 0.3158 0.27 0.4549 0.5035 0.2538 0.2149 0.3576 0.1771 0.3661 0.3178 0.2741 0.306 0.5314 0.3107 0.5201 0.3721 0.3487 0.3957
Eisosomes 0.0008 0.0009 0.0004 0.0002 0.0006 0.0003 0.0004 0.0003 0.0003 0.0001 0.0001 0.0002 0.0004 0.0005 0.0005 0.0003 0.0002 0.0002
Endoplasmic Reticulum 0.0033 0.0018 0.001 0.0005 0.0007 0.0014 0.0009 0.0007 0.0002 0.0003 0.0003 0.0004 0.0013 0.0019 0.0005 0.0025 0.0006 0.0013
Endosome 0.0559 0.0111 0.0148 0.0289 0.0384 0.023 0.013 0.01 0.0058 0.005 0.0176 0.0148 0.0251 0.0219 0.0105 0.0895 0.0172 0.0171
Golgi 0.0169 0.003 0.0093 0.0079 0.0168 0.0036 0.003 0.0029 0.0025 0.0027 0.0054 0.0026 0.0032 0.0052 0.004 0.0139 0.0062 0.0033
Lipid Particles 0.0659 0.0246 0.0265 0.0356 0.0866 0.028 0.0261 0.0136 0.0138 0.0202 0.018 0.0214 0.0544 0.0445 0.0252 0.0277 0.0843 0.0273
Mitochondria 0.0147 0.0022 0.0221 0.0161 0.0349 0.003 0.0026 0.0024 0.0033 0.0018 0.015 0.0043 0.001 0.0043 0.0019 0.0021 0.0252 0.0015
None 0.2722 0.5246 0.1965 0.0905 0.2384 0.5422 0.3437 0.4748 0.2239 0.2455 0.2745 0.4407 0.2057 0.2746 0.1376 0.2371 0.1241 0.3464
Nuclear Periphery 0.0182 0.0012 0.0009 0.0004 0.0006 0.0042 0.0011 0.0011 0.0005 0.0005 0.0003 0.0008 0.0007 0.0013 0.0012 0.0007 0.0004 0.0008
Nucleolus 0.002 0.0014 0.0036 0.003 0.0011 0.0011 0.0042 0.0082 0.0072 0.002 0.0012 0.0021 0.0052 0.0026 0.0028 0.0002 0.0012 0.0006
Nucleus 0.0051 0.0012 0.0025 0.0011 0.0008 0.0018 0.0043 0.0097 0.0029 0.0008 0.0008 0.002 0.0023 0.0039 0.0061 0.0003 0.0006 0.0011
Peroxisomes 0.1006 0.0477 0.0959 0.1871 0.2409 0.0448 0.0954 0.0776 0.0979 0.2309 0.3453 0.0803 0.0686 0.111 0.0956 0.185 0.3028 0.0582
Punctate Nuclear 0.026 0.0182 0.0535 0.0494 0.0033 0.0245 0.0915 0.1219 0.1421 0.0521 0.0273 0.0763 0.0466 0.0538 0.0645 0.0325 0.0055 0.0454
Vacuole 0.0032 0.0014 0.0035 0.0026 0.0056 0.0014 0.0016 0.0019 0.0007 0.0015 0.0019 0.0007 0.0013 0.0033 0.0011 0.0009 0.0025 0.0012
Vacuole Periphery 0.0026 0.0003 0.0014 0.0006 0.0017 0.0009 0.0002 0.0002 0.0001 0.0003 0.0005 0.0002 0.0002 0.0004 0.0002 0.0003 0.0008 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 8.8386 9.1756 3.7826 4.9437 7.763 8.5488 10.9525 8.1749 7.1726 11.6112
Translational Efficiency – – – – – – – – – –

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency –
Standard name
Human Ortholog
Description Spindle positioning factor; orients astral microtubules, connecting them to actin cables at the cortex with Bim1p and Myo2, resulting in proper spindle positioning; targeted for StuBL-dependent degradation at kinetochores by Slx5p-Slx8p, ensuring chromosome transmission fidelity and correct spindle positioning; role in karyogamy; localizes to the shmoo tip, the growing bud-tip, the nucleus, the kinetochore, the spindle and microtubules; homolog of adenomatous polyposis coli
Localization
Cell Percentages —
Cell Cycle Regulation No
Subcompartmental Group N/A

Kar9

Kar9


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Kar9-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available