Standard name
Human Ortholog
Description Putative 2-methylcitrate dehydratase; mitochondrial protein that participates in respiration; induced by diauxic shift; homologous to E. coli PrpD, may take part in the conversion of 2-methylcitrate to 2-methylisocitrate
DOWNLOAD

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0.05 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0.05 0 0.06 0 0 0 – 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Cytoplasm 0.9 0.94 0.89 0.73 0.3 0.24 0.25 0.22 0.21 0.22 0.15 0.15 0.94 0.93 0.91 – 0.87 0.9 0.71 0.72 0.44 0.74 0.71 0.68
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0.12 0.05 0 0 0 0 0 0
Endosome 0 0 0 0 0.17 0 0 0 0 0 0 0 0 0 0.05 – 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Mitochondria 0.17 0 0.1 0.42 0.58 0.78 0.72 0.75 0.85 0.84 0.86 0.9 0 0 0 – 0 0 0.15 0.11 0.32 0.13 0.14 0.11
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0.06 0 0.05 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0.05 0 0.07 0.09 0 0 0.05 0 0 0 0 – 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0.11 0 0.05 0 0 0 0 0 0 0 0 – 0.05 0 0 0 0 0 0 0.06
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 1 1 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 6 9 9 1 1 7
Bud 4 1 3 0 1 5 12 15 11 13 5 13 0 0 0 – 0 0 4 4 6 1 6 6
Bud Neck 2 1 0 0 1 0 0 1 0 3 0 0 0 0 0 – 3 1 2 3 1 0 3 5
Bud Site 0 0 0 0 0 3 1 8 5 2 5 6 0 0 0 – 0 0 – – – – – –
Cell Periphery 0 1 1 2 0 0 2 3 1 0 1 3 0 0 1 – 7 2 2 4 1 0 3 2
Cytoplasm 310 334 148 72 56 60 76 88 61 64 37 30 286 211 262 – 211 217 246 267 80 80 170 188
Endoplasmic Reticulum 0 6 1 0 2 3 1 1 1 2 3 1 2 4 3 – 29 13 2 1 2 0 0 1
Endosome 0 6 0 1 31 4 10 11 0 0 2 0 8 5 15 – 9 7 5 9 6 4 3 1
Golgi 0 0 1 0 1 1 0 0 0 0 0 0 0 1 0 – 0 0 1 1 2 0 2 0
Mitochondria 59 9 16 42 107 198 216 300 240 238 206 181 5 7 5 – 4 4 52 42 59 13 32 31
Nucleus 0 1 1 1 0 4 3 7 2 1 6 1 1 1 0 – 0 1 0 1 0 0 0 0
Nuclear Periphery 0 0 0 0 2 6 2 4 1 0 8 3 0 0 0 – 0 0 0 0 0 0 0 0
Nucleolus 0 1 1 1 0 3 2 2 0 2 3 3 0 0 1 – 0 1 0 1 0 0 0 0
Peroxisomes 0 3 1 0 12 2 14 10 2 1 4 0 4 1 5 – 1 3 0 2 0 0 0 0
SpindlePole 5 1 2 1 10 4 22 36 4 8 12 8 2 1 7 – 6 8 6 7 4 3 6 9
Vac/Vac Membrane 2 3 2 0 20 8 16 16 4 7 5 3 5 2 2 – 13 6 8 7 3 2 4 16
Unique Cell Count 345 357 166 99 186 255 301 399 284 285 240 202 305 226 289 243 242 349 372 185 108 239 279
Labelled Cell Count 382 368 178 120 243 301 377 502 332 341 297 252 313 233 301 283 263 349 372 185 108 239 279


Cytoplasm, Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.0 4.0 4.3 3.9 4.4 3.9 3.3 3.7 3.3 2.9 3.5 3.1 4.4 4.3 4.4 4.7 5.8 5.4 4.1 4.1 4.7
Std Deviation (1e-4) 0.4 0.9 2.5 1.7 1.4 2.2 1.1 1.8 1.6 1.2 1.7 1.0 1.2 1.3 1.4 1.3 1.7 1.6 1.9 1.7 1.2
Intensity Change (Log2) – – – -0.14 0.02 -0.16 -0.39 -0.24 -0.38 -0.6 -0.29 -0.5 0.02 0.0 0.03 0.11 0.42 0.32 -0.08 -0.06 0.13


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – 0 –
Bud Neck – – – – – – – – – – – – – 0 –
Bud Site – – – – – – – – – – – – – 0 –
Cell Periphery – – – – – – – – – – – – – 0 –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – 0 –
Endosome – – – – – – – – – – – – – 0 –
Golgi – – – – – – – – – – – – – 0 –
Mitochondria – – – – – – – – – – – – – 0 –
Nuclear Periphery – – – – – – – – – – – – – 0 –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – 0 –
Peroxisomes – – – – – – – – – – – – – 0 –
SpindlePole – – – – – – – – – – – – – 0 –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – 0 –
Cytoplasm – – – – – – – – – – – – – 0 –
Nucleus – – – – – – – – – – – – – 0 –
Vacuole – – – – – – – – – – – – – 2.2 –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.2627 1.6193 1.0842 1.8484 0.551 1.1738 3.1358 2.9258 3.0431 3.131 4.0926 4.2064 5.3896 5.5029 5.1326 4.9028 4.0 4.7146
Actin 0.0766 0.0647 0.0171 0.0024 0.0344 0.0071 0.0409 0.0254 0.0137 0.0223 0.0637 0.0137 0.0021 0.0232 0.0307 0.0268 0.0048 0.0191
Bud 0.0012 0.0009 0.0027 0.0016 0.0013 0.0028 0.002 0.0041 0.0082 0.0047 0.0078 0.0029 0.0002 0.0014 0.0013 0.0009 0.0003 0.0014
Bud Neck 0.0019 0.003 0.0041 0.0008 0.0017 0.0073 0.0016 0.0009 0.001 0.0007 0.0007 0.0025 0.0007 0.0012 0.0031 0.0001 0 0.0156
Bud Periphery 0.0036 0.0014 0.0069 0.0097 0.0033 0.0068 0.004 0.0044 0.027 0.0267 0.0253 0.0098 0.0004 0.0027 0.0033 0.0053 0.0014 0.006
Bud Site 0.0096 0.0081 0.0288 0.0015 0.0024 0.0033 0.0075 0.0156 0.0114 0.0051 0.0022 0.0013 0.0011 0.0112 0.0093 0.0004 0.0002 0.0011
Cell Periphery 0.0038 0.0014 0.0039 0.0321 0.0055 0.0016 0.0014 0.003 0.0025 0.0019 0.001 0.0014 0.0002 0.0065 0.0019 0.0002 0.0001 0.0038
Cytoplasm 0.0164 0.0076 0.0402 0.0163 0.057 0.0272 0.0256 0.0243 0.0356 0.0359 0.0135 0.0257 0.0115 0.0199 0.0072 0.0033 0.0001 0.0178
Cytoplasmic Foci 0.0843 0.0889 0.0394 0.0659 0.0417 0.0583 0.0744 0.0528 0.0711 0.0356 0.0449 0.0733 0.0395 0.0579 0.0559 0.0224 0.0033 0.0501
Eisosomes 0.0031 0.0022 0.0017 0.0006 0.0012 0.0005 0.0017 0.002 0.002 0.0015 0.0004 0.0006 0.0002 0.0007 0.0009 0.0013 0.0002 0.0019
Endoplasmic Reticulum 0.0017 0.0059 0.0052 0.0034 0.0009 0.0016 0.0037 0.001 0.0023 0.0012 0.0019 0.0022 0.0007 0.0018 0.0013 0.0008 0 0.0025
Endosome 0.0574 0.0394 0.0604 0.0707 0.0648 0.0521 0.0799 0.072 0.053 0.0491 0.0525 0.0454 0.0766 0.075 0.0426 0.0337 0.0043 0.0449
Golgi 0.0401 0.0336 0.045 0.0166 0.0603 0.0426 0.0464 0.0541 0.0311 0.036 0.048 0.0412 0.0421 0.0554 0.0489 0.0458 0.0138 0.0383
Lipid Particles 0.0491 0.0391 0.0335 0.035 0.0087 0.0468 0.0487 0.0305 0.0158 0.0085 0.0158 0.0403 0.0451 0.0154 0.015 0.0015 0.0011 0.0254
Mitochondria 0.4557 0.5322 0.4922 0.6324 0.6151 0.5909 0.5025 0.5453 0.549 0.6047 0.5822 0.5872 0.6301 0.5698 0.7001 0.7866 0.9431 0.689
None 0.0675 0.0101 0.1295 0.0685 0.0746 0.0451 0.063 0.0296 0.073 0.1229 0.0759 0.0427 0.0543 0.043 0.0359 0.059 0.0001 0.0117
Nuclear Periphery 0.0042 0.0047 0.0015 0.0009 0.0002 0.0007 0.0069 0.0004 0.0046 0.0008 0.0113 0.0013 0.0022 0.0009 0.0008 0.0006 0 0.0005
Nucleolus 0.0017 0.0007 0.001 0.0008 0.0002 0.001 0.003 0.0007 0.0011 0.0005 0.0007 0.0006 0.0042 0.0006 0.0008 0.0001 0 0.0003
Nucleus 0.0054 0.0007 0.0013 0.0008 0.0002 0.0007 0.0036 0.0004 0.001 0.0006 0.0048 0.0007 0.0083 0.0007 0.0007 0.0002 0 0.0003
Peroxisomes 0.0674 0.1159 0.0735 0.0283 0.0224 0.0963 0.0626 0.1251 0.0855 0.0323 0.0307 0.0967 0.0466 0.0886 0.0263 0.0094 0.026 0.0634
Punctate Nuclear 0.0084 0.001 0.001 0.0007 0.0004 0.0011 0.0085 0.0003 0.0015 0.0025 0.0121 0.0012 0.0282 0.0004 0.0046 0.0002 0 0.0003
Vacuole 0.0285 0.0309 0.0078 0.0079 0.0022 0.0036 0.0077 0.0055 0.0057 0.0041 0.0025 0.0054 0.0034 0.0177 0.0059 0.0005 0.0004 0.0032
Vacuole Periphery 0.0123 0.0076 0.0033 0.0031 0.0017 0.0026 0.0044 0.0027 0.004 0.0024 0.0021 0.0038 0.0024 0.0059 0.0035 0.0009 0.0008 0.0034

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 6.7584 6.3596 5.191 11.8881 4.6119 17.7755 16.0755 29.2947 25.486 13.8052
Translational Efficiency 1.1803 0.9261 1.7108 0.9918 1.3356 1.4333 1.1125 0.9247 1.061 0.8877

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✔
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
128 1548 1939 1782 1251 1744 216 1578 1379 3292 2155 3360

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 615.10 675.85 708.81 874.08 611.08 663.61 894.45 827.81 611.45 669.37 727.42 852.35
Standard Deviation 74.18 84.44 80.61 163.17 64.34 92.91 97.73 161.32 65.33 89.24 99.56 163.94
Intensity Change Log 2 — 0.135882 0.204578 0.506944 — 0.118974 0.549640 0.437938 — 0.127481 0.386838 0.472967

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000027 0.000720 0.000054 0.011908 0.000095 0.000549 0.004617 0.003134 0.000089 0.000629 0.000512 0.007787
Bud Neck 0.001209 0.067929 0.007080 0.020164 0.015312 0.067547 0.004862 0.030851 0.014003 0.067727 0.006858 0.025183
Bud Site 0.000138 0.033089 0.004708 0.126277* 0.002333 0.031449 0.014369 0.067118 0.002129 0.032220 0.005677 0.098493
Cell Periphery 0.000162 0.000179 0.001421 0.000491 0.000118 0.000223 0.000229 0.000519 0.000122 0.000202 0.001301 0.000504
Cytoplasm 0.331504* 0.252435* 0.317216* 0.121813* 0.449741* 0.283214* 0.264875* 0.145344* 0.438766* 0.268741* 0.311970* 0.132864*
Cytoplasmic Foci 0.104531 0.309824* 0.021354 0.075884 0.222163* 0.308933* 0.046745 0.082037 0.211244* 0.309352* 0.023899 0.078774
Eisosomes 0.000107 0.000211 0.000336 0.000121 0.000134 0.000218 0.000133 0.000121 0.000131 0.000215 0.000316 0.000121
Endoplasmic Reticulum 0.000214 0.000795 0.000771 0.002209 0.000570 0.000722 0.006175 0.002348 0.000537 0.000756 0.001312 0.002274
Endosome 0.001881 0.054201 0.002046 0.045004 0.010749 0.040287 0.019386 0.068583 0.009926 0.046830 0.003784 0.056078
Golgi 0.000785 0.019001 0.000109 0.064539 0.001227 0.016941 0.004470 0.046241 0.001186 0.017910 0.000546 0.055946
Lipid Particles 0.004995 0.010328 0.002644 0.001820 0.010616 0.010577 0.006103 0.008482 0.010095 0.010460 0.002991 0.004949
Mitochondria 0.000898 0.052070 0.000675 0.242541* 0.004736 0.041876 0.010520 0.211274* 0.004380 0.046670 0.001661 0.227856*
Mitotic Spindle 0.000418 0.006526 0.000994 0.108507* 0.002425 0.007746 0.017267 0.079932 0.002238 0.007173 0.002625 0.095087
None 0.007381 0.002390 0.017763 0.001582 0.004548 0.003243 0.002964 0.002023 0.004811 0.002842 0.016280 0.001789
Nuclear Periphery 0.000653 0.000425 0.000961 0.000458 0.000617 0.000391 0.001883 0.001856 0.000620 0.000407 0.001054 0.001115
Nuclear Periphery Foci 0.000091 0.000320 0.000196 0.000531 0.000375 0.000282 0.004831 0.001431 0.000348 0.000300 0.000661 0.000953
Nucleolus 0.002616 0.002658 0.002115 0.000527 0.002148 0.002625 0.001846 0.002845 0.002192 0.002641 0.002088 0.001615
Nucleus 0.529608* 0.057713 0.596175* 0.026557 0.225854* 0.070569 0.414654* 0.064035 0.254049* 0.064524 0.577981* 0.044159
Peroxisomes 0.005082 0.045087 0.000976 0.057017 0.007785 0.040390 0.009872 0.030670 0.007534 0.042599 0.001868 0.044643
Vacuole 0.007551 0.078586 0.022124 0.079295 0.037497 0.068744 0.162299 0.128009 0.034717 0.073372 0.036174 0.102173
Vacuole Periphery 0.000147 0.005512 0.000281 0.012756 0.000958 0.003474 0.001902 0.023149 0.000883 0.004432 0.000443 0.017637

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -9.26 -2.04 -9.68 -9.05 -9.64 -6.01 -1.87 -4.45 -3.73 0.15 -10.06 -1.73 -10.54 -9.72 -9.80
Bud Neck -20.95* -6.35 -14.71 13.50 -8.56 -15.42 7.10 -6.15 9.74 -11.50 -21.23 4.93 -6.79 16.49 -12.58
Bud Site -18.37 -5.01 -26.50 -17.87 -24.96* -13.35 -2.71 -18.05 -8.24 -10.55 -20.69 -3.48 -31.18 -19.02 -28.86*
Cell Periphery -0.51 -7.66 -7.07 -7.67 5.72 -2.38 -4.75 -6.93 -3.88 -4.67 -3.21 -8.06 -10.96 -6.98 5.47
Cytoplasm 3.36 0.77 8.10 13.16 21.73 16.94 11.91 30.24 13.87 4.48 20.10 14.74 35.23 19.37 22.79
Cytoplasmic Foci -16.61 7.60 2.81 37.09 -17.14 -10.43 25.22 21.03 34.94 -8.25 -14.12 33.05 22.65 50.96 -22.68
Eisosomes -7.99 -4.68 -0.89 10.08 4.55 -6.47 -0.76 1.67 7.33 1.80 -9.27 -4.25 1.99 11.64 4.59
Endoplasmic Reticulum -5.57 -6.59 -7.73 -5.63 -5.14 -0.88 -9.91 -9.45 -7.02 6.75 -1.98 -7.87 -10.33 -8.56 -4.47
Endosome -20.40 -0.36 -18.95 4.32 -20.12 -14.23 -0.90 -16.87 -6.68 -12.28 -20.70 6.55 -20.99 -1.97 -27.40*
Golgi -12.75 0.97 -18.04 -11.65 -18.71 -11.45 -0.88 -14.95 -8.23 -13.07 -17.71 4.31 -23.58 -14.24 -24.01*
Lipid Particles -4.93 3.02 3.92 10.12 1.66 -0.02 4.58 1.90 2.14 -2.31 -0.43 8.86 5.81 8.24 -3.88
Mitochondria -16.07 0.53 -28.57* -20.19 -28.64* -13.09 -0.19 -24.33 -18.47 -20.92* -19.36 4.03 -37.00* -27.50 -37.74*
Mitotic Spindle -7.31 -1.11 -22.08 -20.59 -21.95 -3.43 -1.76 -16.40 -15.21 -9.52 -4.16 0.17 -26.35 -25.39 -26.98
None 2.13 -3.29 2.46 1.39 8.35 1.56 2.58 3.30 1.90 0.71 2.79 -6.27 4.22 2.35 8.46
Nuclear Periphery 1.71 -3.08 0.29 -1.77 3.96 2.96 -9.44 -4.17 -4.98 0.77 2.74 -5.10 -3.90 -5.33 -0.32
Nuclear Periphery Foci -5.02 -4.63 -10.00 -4.16 -6.72 0.78 -3.80 -3.87 -4.29 2.76 0.46 -2.26 -4.34 -5.23 -1.92
Nucleolus -0.14 0.40 2.05 6.06 3.83 -1.04 1.27 -1.57 -0.75 -2.88 -1.15 0.12 0.90 3.30 0.83
Nucleus 18.09 -2.49 18.77 4.51 78.55* 24.11 -8.74 21.93 -0.19 15.91 29.05 -36.76 29.93 3.06 77.09*
Peroxisomes -8.56 0.95 -9.07 -1.89 -16.37 -15.19 0.68 -9.11 3.39 -6.91 -20.75 6.35 -15.82 0.26 -20.02
Vacuole -22.54 -10.40 -23.60 -5.24 -18.21 -10.05 -11.07 -20.80 -13.59 4.27 -15.35 -3.56 -25.33 -13.36 -19.64
Vacuole Periphery -14.14 -2.60 -13.18 -6.39 -13.02 -8.25 -0.57 -13.59 -11.77 -13.15 -13.41 3.60 -17.92 -13.41 -18.73
Standard name
Human Ortholog
Description Putative 2-methylcitrate dehydratase; mitochondrial protein that participates in respiration; induced by diauxic shift; homologous to E. coli PrpD, may take part in the conversion of 2-methylcitrate to 2-methylisocitrate
Localization
Cell Percentages —
Cell Cycle Regulation No
Subcompartmental Group N/A

Pdh1

Pdh1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Pdh1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available