ORF | |
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Human Ortholog | |
Description | Putative sulfate permease; physically interacts with Hsp82p; green fluorescent protein (GFP)-fusion protein localizes to the ER; YPR003C is not an essential gene |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | – | – | 1 | – | – | 0 | 1 | 3 | 9 | 1 | 3 | 0 |
Bud | 0 | 4 | 2 | 2 | 0 | 5 | 10 | 10 | – | 8 | 11 | 11 | – | – | 0 | – | – | 0 | 0 | 1 | 3 | 1 | 1 | 2 |
Bud Neck | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | – | 0 | 0 | 1 | – | – | 0 | – | – | 0 | 3 | 4 | 3 | 1 | 1 | 0 |
Bud Site | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | – | 0 | 0 | 0 | – | – | 0 | – | – | 0 | – | – | – | – | – | – |
Cell Periphery | 1 | 0 | 0 | 0 | 2 | 0 | 2 | 4 | – | 2 | 4 | 3 | – | – | 0 | – | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 207 | 163 | 229 | 202 | 215 | 287 | 416 | 389 | – | 263 | 288 | 276 | – | – | 145 | – | – | 189 | 69 | 64 | 45 | 25 | 54 | 45 |
Endoplasmic Reticulum | 9 | 5 | 3 | 0 | 2 | 2 | 6 | 3 | – | 2 | 6 | 9 | – | – | 4 | – | – | 12 | 60 | 25 | 81 | 17 | 26 | 29 |
Endosome | 6 | 1 | 29 | 6 | 14 | 4 | 4 | 8 | – | 2 | 1 | 2 | – | – | 0 | – | – | 5 | 14 | 17 | 35 | 3 | 9 | 8 |
Golgi | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | – | – | 0 | – | – | 1 | 3 | 0 | 3 | 1 | 1 | 0 |
Mitochondria | 3 | 11 | 11 | 21 | 17 | 95 | 195 | 196 | – | 228 | 277 | 245 | – | – | 1 | – | – | 8 | 38 | 38 | 52 | 24 | 29 | 32 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 4 | – | 1 | 1 | 3 | – | – | 0 | – | – | 2 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | – | 1 | 0 | 1 | – | – | 0 | – | – | 0 | 1 | 0 | 2 | 1 | 0 | 0 |
Nucleolus | 0 | 0 | 1 | 1 | 0 | 1 | 0 | 3 | – | 1 | 2 | 0 | – | – | 0 | – | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 2 | 0 | 1 | 0 | 0 | 0 | – | 0 | 0 | 0 | – | – | 0 | – | – | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
SpindlePole | 0 | 0 | 2 | 0 | 1 | 1 | 3 | 0 | – | 2 | 1 | 3 | – | – | 0 | – | – | 0 | 0 | 0 | 1 | 0 | 1 | 1 |
Vac/Vac Membrane | 14 | 14 | 25 | 4 | 16 | 6 | 31 | 10 | – | 8 | 18 | 13 | – | – | 1 | – | – | 8 | 65 | 68 | 57 | 47 | 91 | 75 |
Unique Cell Count | 235 | 187 | 277 | 216 | 245 | 327 | 505 | 514 | 387 | 455 | 443 | 151 | 202 | 262 | 229 | 301 | 131 | 226 | 201 | |||||
Labelled Cell Count | 242 | 201 | 304 | 236 | 268 | 401 | 667 | 629 | 518 | 609 | 567 | 152 | 225 | 262 | 229 | 301 | 131 | 226 | 201 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 4.7 | 4.8 | 4.6 | 4.4 | 4.9 | 3.6 | 3.6 | 3.8 | – | 3.3 | 3.3 | 3.3 | – | – | 5.9 | 4.3 | 4.5 | 5.5 | 5.5 | 6.3 | 6.7 |
Std Deviation (1e-4) | 0.5 | 1.1 | 1.7 | 1.0 | 2.6 | 0.8 | 1.1 | 1.2 | – | 1.0 | 0.8 | 0.9 | – | – | 1.0 | 0.9 | 0.8 | 1.3 | 1.5 | 1.9 | 2.1 |
Intensity Change (Log2) | – | – | – | -0.08 | 0.08 | -0.35 | -0.37 | -0.29 | – | -0.5 | -0.46 | -0.49 | – | – | 0.35 | -0.1 | -0.05 | 0.24 | 0.26 | 0.46 | 0.54 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | -2.2 |
R1 | R2 | R3 | ||||||||||||||||
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G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 2.1211 | 1.7363 | 1.5799 | 1.7792 | 1.5875 | 1.4137 | 2.0638 | 2.887 | 2.8888 | 2.9487 | 2.266 | 2.8199 | 2.001 | 2.9573 | 2.595 | 2.6902 | 3.3795 | 2.76 |
Actin | 0.0452 | 0.0001 | 0.0107 | 0.01 | 0.0126 | 0.0019 | 0.0079 | 0.0003 | 0.0192 | 0.0024 | 0.0001 | 0.0007 | 0.0159 | 0.0001 | 0.0004 | 0.0013 | 0 | 0.002 |
Bud | 0.001 | 0.0001 | 0.001 | 0.0044 | 0.0051 | 0.0004 | 0.0005 | 0.0002 | 0.0007 | 0.0013 | 0.0001 | 0.0005 | 0.0004 | 0.0001 | 0.0003 | 0.0002 | 0.0002 | 0.0002 |
Bud Neck | 0.0007 | 0.0002 | 0.0003 | 0.0114 | 0.0007 | 0.0011 | 0.0009 | 0.0002 | 0.0015 | 0.0005 | 0.0004 | 0.0012 | 0.0002 | 0.0001 | 0.0002 | 0.0013 | 0.0003 | 0.0012 |
Bud Periphery | 0.0013 | 0.0002 | 0.0014 | 0.0034 | 0.007 | 0.0004 | 0.0011 | 0.0003 | 0.0012 | 0.0018 | 0.0002 | 0.001 | 0.0005 | 0.0001 | 0.0003 | 0.0002 | 0.0002 | 0.0002 |
Bud Site | 0.0023 | 0.0019 | 0.0021 | 0.0059 | 0.0186 | 0.0014 | 0.002 | 0.0013 | 0.0027 | 0.0015 | 0.0002 | 0.0003 | 0.001 | 0.0008 | 0.0023 | 0.0004 | 0.0002 | 0.0003 |
Cell Periphery | 0.0004 | 0.0005 | 0.0005 | 0.0003 | 0.0012 | 0.0002 | 0.0007 | 0.001 | 0.0005 | 0.0008 | 0.0007 | 0.0007 | 0.0008 | 0.0007 | 0.0007 | 0.0006 | 0.0003 | 0.0005 |
Cytoplasm | 0.3029 | 0.4674 | 0.3533 | 0.4677 | 0.4345 | 0.5089 | 0.3783 | 0.3708 | 0.3494 | 0.3169 | 0.524 | 0.4554 | 0.3 | 0.5507 | 0.419 | 0.497 | 0.6697 | 0.4679 |
Cytoplasmic Foci | 0.0501 | 0.0345 | 0.0501 | 0.0432 | 0.0259 | 0.0441 | 0.0694 | 0.0389 | 0.061 | 0.0467 | 0.0218 | 0.0373 | 0.0755 | 0.0251 | 0.0489 | 0.0533 | 0.0205 | 0.0309 |
Eisosomes | 0.0002 | 0 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0003 | 0.0001 | 0.0002 | 0 | 0 | 0 | 0.0002 | 0 | 0.0001 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0.0729 | 0.0685 | 0.0415 | 0.077 | 0.0352 | 0.0658 | 0.0558 | 0.0863 | 0.0405 | 0.0849 | 0.0386 | 0.077 | 0.0153 | 0.0825 | 0.0319 | 0.0813 | 0.0716 | 0.0798 |
Endosome | 0.1217 | 0.0917 | 0.1735 | 0.0995 | 0.1627 | 0.1361 | 0.1422 | 0.1252 | 0.1409 | 0.1874 | 0.1434 | 0.1254 | 0.0402 | 0.0858 | 0.1039 | 0.2197 | 0.0799 | 0.1044 |
Golgi | 0.0104 | 0.0015 | 0.0088 | 0.0271 | 0.0146 | 0.0032 | 0.0088 | 0.002 | 0.0063 | 0.0049 | 0.0014 | 0.0053 | 0.0124 | 0.0019 | 0.0065 | 0.0035 | 0.0007 | 0.008 |
Lipid Particles | 0.0126 | 0.0074 | 0.016 | 0.0057 | 0.0097 | 0.0101 | 0.0239 | 0.0147 | 0.0122 | 0.0044 | 0.0112 | 0.0083 | 0.0349 | 0.0093 | 0.0116 | 0.013 | 0.0044 | 0.0108 |
Mitochondria | 0.0068 | 0.0013 | 0.009 | 0.0087 | 0.0155 | 0.0039 | 0.0108 | 0.001 | 0.0025 | 0.0039 | 0.0009 | 0.0116 | 0.0022 | 0.0011 | 0.0015 | 0.0005 | 0.0011 | 0.0025 |
None | 0.1988 | 0.2357 | 0.2144 | 0.1412 | 0.1766 | 0.148 | 0.234 | 0.2854 | 0.3066 | 0.2468 | 0.1754 | 0.2005 | 0.4052 | 0.1894 | 0.2976 | 0.0494 | 0.0375 | 0.2016 |
Nuclear Periphery | 0.0706 | 0.015 | 0.0184 | 0.0231 | 0.0148 | 0.0105 | 0.0168 | 0.014 | 0.0088 | 0.0102 | 0.0059 | 0.0097 | 0.0035 | 0.0043 | 0.0088 | 0.0078 | 0.0355 | 0.0092 |
Nucleolus | 0.0042 | 0.0004 | 0.0008 | 0.0006 | 0.0008 | 0.0003 | 0.0006 | 0.0004 | 0.0008 | 0.0002 | 0.0001 | 0.0002 | 0.0003 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0.0002 |
Nucleus | 0.0439 | 0.0044 | 0.0053 | 0.0124 | 0.0073 | 0.0044 | 0.0033 | 0.0055 | 0.0035 | 0.0021 | 0.0025 | 0.0032 | 0.0006 | 0.0017 | 0.0022 | 0.0019 | 0.0059 | 0.0026 |
Peroxisomes | 0.0044 | 0.0004 | 0.0105 | 0.0036 | 0.0022 | 0.0014 | 0.0035 | 0.0002 | 0.0017 | 0.0269 | 0.0001 | 0.0006 | 0.0689 | 0.0001 | 0.008 | 0.001 | 0.0001 | 0.0013 |
Punctate Nuclear | 0.0213 | 0.0012 | 0.0037 | 0.0036 | 0.0092 | 0.0046 | 0.0016 | 0.0028 | 0.0034 | 0.0008 | 0.0005 | 0.001 | 0.0133 | 0.0005 | 0.0011 | 0.0009 | 0.0004 | 0.0006 |
Vacuole | 0.0218 | 0.0611 | 0.0699 | 0.0441 | 0.0377 | 0.0475 | 0.0337 | 0.0466 | 0.032 | 0.0486 | 0.0666 | 0.0556 | 0.0084 | 0.0433 | 0.0498 | 0.0621 | 0.0609 | 0.0723 |
Vacuole Periphery | 0.0063 | 0.0065 | 0.0087 | 0.0069 | 0.0079 | 0.0059 | 0.0038 | 0.0028 | 0.0044 | 0.0069 | 0.0059 | 0.0044 | 0.0005 | 0.0022 | 0.0048 | 0.0046 | 0.0104 | 0.0035 |
R1 | R2 | |||||||||
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G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 8.5252 | 9.1452 | 8.0787 | 11.3574 | 4.1055 | 9.7377 | 8.8064 | 9.9557 | 9.8507 | 9.1425 |
Translational Efficiency | 0.4835 | 0.5141 | 0.6801 | 0.4085 | 0.8208 | 0.5186 | 0.6948 | 0.6571 | 0.7478 | 0.5856 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
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37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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