Standard name
Human Ortholog
Description Essential helicase component of heterohexameric MCM2-7 complexes; MCM2-7 complexes bind pre-replication complexes on DNA and melt DNA prior to replication; forms an Mcm4p-6p-7p subcomplex; shows nuclear accumulation in G1; homolog of S. pombe Cdc21p
DOWNLOAD

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0.09 0.06 0 0.05 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.26 0.17 0.3 0.25 0.37 0.31 0.28 0.19 0.18 – 0.15 0.1 0.09 0.16 0.22 0.39 0.34 0.35 0.13 0.17 0.26 0.11 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0.07 0 0 0 0.08 0 0 0 0 – 0 0 0.05 0 0 0 0 0.05 0 0 0 0 0 0.09
Golgi 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0.07 0.08 0.14 0.08 0.26 0.23 0.18 0.3 – 0.34 0.41 0 0 0.08 0 0 0 0 0 0 0 0 0
Nucleus 0.51 0.68 0.56 0.57 0.34 0.27 0.35 0.42 0.37 – 0.4 0.36 0.77 0.6 0.4 0.42 0.47 0.35 0.4 0.62 0.51 0.58 0.5 0.3
Nuclear Periphery 0 0 0 0 0 0 0 0.05 0.06 – 0.06 0 0 0 0.06 0 0 0 0 0 0 0 0 0
Nucleolus 0.12 0.24 0.07 0.12 0.13 0.28 0.24 0.24 0.28 – 0.27 0.35 0.11 0.2 0.24 0.08 0.05 0.11 0.25 0.06 0.09 0.15 0.23 0.29
Peroxisomes 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06
Vac/Vac Membrane 0.08 0 0.05 0.05 0.1 0 0.06 0.05 0.05 – 0.07 0.07 0 0.07 0.08 0.16 0.13 0.17 0 0 0 0 0.12 0.17
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 1 0 0 1 0 – 1 1 0 0 1 1 0 1 0 0 0 0 0 0
Bud 1 1 5 4 4 7 7 12 13 – 11 13 0 0 1 0 1 0 13 4 5 7 7 2
Bud Neck 0 0 0 0 0 0 0 3 2 – 1 3 0 0 1 0 0 0 0 0 0 2 0 0
Bud Site 0 0 0 0 0 4 5 6 4 – 3 16 0 0 0 0 0 0 – – – – – –
Cell Periphery 2 0 0 0 2 0 1 4 2 – 1 5 0 0 1 0 2 4 0 0 0 0 0 0
Cytoplasm 34 10 36 74 152 107 157 107 60 – 57 45 20 60 75 147 75 111 18 14 34 18 4 0
Endoplasmic Reticulum 1 0 0 0 2 0 1 3 2 – 1 1 0 0 2 10 6 4 0 0 1 1 1 3
Endosome 9 1 3 5 33 11 16 19 5 – 2 4 12 16 14 7 5 16 4 2 2 1 13 31
Golgi 1 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 4 2 0 0 0 0 1 2
Mitochondria 2 4 10 42 33 91 128 99 103 – 130 193 2 5 27 6 6 6 0 0 1 0 2 4
Nucleus 66 40 68 169 140 94 198 234 126 – 151 171 180 220 133 158 104 110 57 52 68 95 154 105
Nuclear Periphery 1 0 1 2 13 15 25 29 21 – 21 11 5 13 19 8 1 10 1 0 0 0 1 7
Nucleolus 16 14 8 36 55 95 132 135 96 – 101 166 25 72 80 31 11 36 35 4 11 25 70 103
Peroxisomes 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 2 5 5 1 3 3 – 2 2 0 0 0 0 0 6 6 0 2 1 9 21
Vac/Vac Membrane 10 1 6 15 42 11 31 27 17 – 28 31 8 27 28 61 29 53 3 1 3 6 38 60
Unique Cell Count 130 59 122 297 411 344 560 560 342 379 471 234 369 334 380 219 317 144 85 133 165 311 350
Labelled Cell Count 143 71 137 349 482 440 702 682 454 510 662 252 413 382 429 244 359 144 85 133 165 311 350


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 11.8 9.1 7.0 7.3 7.3 6.9 6.9 7.3 6.7 – 6.5 6.3 11.0 10.5 9.0 11.4 13.5 13.4 9.9 9.9 11.0
Std Deviation (1e-4) 2.1 2.1 1.4 1.9 1.7 1.7 1.6 2.2 2.3 – 1.7 1.6 2.7 2.1 2.0 3.2 3.9 4.2 2.1 2.3 2.2
Intensity Change (Log2) – – – 0.05 0.06 -0.04 -0.03 0.05 -0.08 – -0.11 -0.15 0.64 0.57 0.36 0.7 0.94 0.93 0.5 0.48 0.64


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Cytoplasm -1.0 1.5 0.3 -0.3 -2.6 -2.8 – -3.6 -5.7 -5.2 -3.2 -1.6 1.8 0.9 1.1
Endoplasmic Reticulum 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Endosome 0 2.2 0 0 0 0 – 0 0 1.2 0 0 0 0 1.2
Golgi 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Mitochondria 1.7 -0.1 4.2 3.7 2.6 4.8 – 5.6 6.8 0 0 0 0 -2.3 0
Nucleus 0.2 -4.3 -5.7 -4.2 -2.8 -3.6 – -3.1 -3.9 4.1 0.8 -3.0 -2.7 -1.5 -4.0
Nuclear Periphery 0 0 0 0 2.1 2.4 – 2.2 0 0 0 2.2 0 0 0
Nucleolus 1.7 2.1 4.8 4.2 4.3 4.9 – 4.7 6.2 1.3 3.4 4.2 0.6 -0.6 1.5
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Vacuole 0.1 1.8 0 0.3 0 0 – 0.9 0.7 0 0.9 1.2 3.1 2.4 3.2

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 6.9191 7.4645 6.282 5.7585 6.0864 6.8658 8.8444 9.2233 7.6604 6.9333 7.4017 8.6862 5.7557 5.7759 4.6177 4.4691 5.1086 5.3642
Actin 0.0232 0.0011 0.007 0.0083 0.0378 0.0004 0.0005 0.001 0.0139 0.0155 0.0021 0.0009 0.0191 0.0017 0.0083 0.0154 0.0035 0.0008
Bud 0.0006 0.0209 0.0645 0.0315 0.0075 0.0106 0.0007 0.0228 0.0788 0.0195 0.0394 0.0033 0.0005 0.0079 0.0597 0.1215 0.0417 0.0031
Bud Neck 0.0015 0.0024 0.0054 0.0072 0.0365 0.0084 0.0002 0.0014 0.0047 0.0115 0.0031 0.005 0.0011 0.0021 0.0055 0.0056 0.0033 0.0053
Bud Periphery 0.0008 0.0112 0.0452 0.0304 0.007 0.0165 0.0015 0.0118 0.0511 0.0086 0.0217 0.0023 0.001 0.0054 0.0512 0.0987 0.0417 0.004
Bud Site 0.0017 0.0551 0.0392 0.0348 0.0103 0.003 0.0006 0.0326 0.0322 0.0073 0.0053 0.001 0.0016 0.0154 0.0318 0.0247 0.0094 0.0028
Cell Periphery 0.0003 0.0024 0.0033 0.0047 0.002 0.0011 0.0001 0.0014 0.0022 0.0009 0.0004 0.0003 0.0004 0.0012 0.0032 0.0026 0.0011 0.0004
Cytoplasm 0.017 0.1103 0.3355 0.5402 0.5202 0.0028 0.0083 0.0936 0.3095 0.5655 0.6077 0.066 0.0038 0.1151 0.3599 0.477 0.6716 0.0622
Cytoplasmic Foci 0.0262 0.0046 0.0244 0.0311 0.0286 0.0001 0.0033 0.005 0.0265 0.0551 0.0142 0.0015 0.0039 0.0035 0.0329 0.0236 0.022 0.0006
Eisosomes 0.0003 0 0.0002 0.0003 0.0003 0 0 0 0 0.0001 0 0 0.0001 0 0.0001 0 0 0
Endoplasmic Reticulum 0.0055 0.0106 0.0047 0.0067 0.0066 0.0001 0.0013 0.0018 0.0047 0.0058 0.0167 0.0014 0.001 0.0024 0.0122 0.0077 0.004 0.0008
Endosome 0.0425 0.0088 0.0152 0.0358 0.0529 0.0002 0.0092 0.0036 0.0215 0.0613 0.0145 0.0071 0.0066 0.003 0.0234 0.0237 0.023 0.0018
Golgi 0.0067 0.0013 0.0022 0.0026 0.0124 0.0001 0.0004 0.0003 0.0053 0.0127 0.0011 0.002 0.0012 0.0004 0.0024 0.0014 0.0011 0.0001
Lipid Particles 0.0132 0.0008 0.0092 0.0132 0.0331 0.0001 0.003 0.0007 0.0039 0.03 0.0007 0.0011 0.0033 0.0007 0.0088 0.0012 0.0004 0.0001
Mitochondria 0.0045 0.0011 0.0129 0.0283 0.011 0.0008 0.0017 0.0021 0.008 0.004 0.0012 0.0071 0.0029 0.0013 0.0042 0.0036 0.0016 0.0009
None 0.0037 0.0009 0.0063 0.0052 0.0031 0.0001 0.0029 0.0012 0.0029 0.0033 0.0021 0.0008 0.0012 0.0014 0.0049 0.0055 0.0046 0.0003
Nuclear Periphery 0.0143 0.0064 0.0222 0.0074 0.0182 0.0017 0.0298 0.0065 0.022 0.0099 0.0086 0.0052 0.0082 0.0365 0.0185 0.0117 0.0058 0.0036
Nucleolus 0.0818 0.0974 0.0449 0.0479 0.035 0.2296 0.0313 0.04 0.0238 0.0084 0.0213 0.1092 0.0384 0.0426 0.0318 0.0105 0.0244 0.1393
Nucleus 0.7203 0.6431 0.2798 0.0771 0.0406 0.7132 0.8965 0.7228 0.3198 0.108 0.1729 0.779 0.8883 0.7024 0.2412 0.1042 0.0894 0.7653
Peroxisomes 0.0035 0.0002 0.0102 0.0024 0.0232 0.0001 0.0001 0.0003 0.0049 0.002 0.0002 0.0002 0.0044 0.0002 0.0173 0.0013 0.0005 0.0001
Punctate Nuclear 0.0228 0.0042 0.02 0.0061 0.0126 0.0062 0.0036 0.0147 0.0185 0.0205 0.0518 0.0017 0.0096 0.0465 0.0412 0.0066 0.0029 0.005
Vacuole 0.0073 0.0163 0.0421 0.0729 0.092 0.0042 0.0033 0.0306 0.0387 0.042 0.0133 0.0038 0.0028 0.0087 0.0363 0.0468 0.0429 0.0029
Vacuole Periphery 0.0021 0.0011 0.0056 0.0061 0.009 0.0005 0.0015 0.0058 0.007 0.0081 0.0017 0.0013 0.0007 0.0016 0.0054 0.0065 0.0052 0.0007

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 14.5703 12.6729 28.4728 43.2682 22.465 24.7951 18.0636 30.6294 37.2433 30.3152
Translational Efficiency 0.7872 0.613 1.1431 0.7926 0.7621 0.676 0.8404 0.7217 0.7997 0.7421

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✔
Gene Expression ✔
Translational Efficiency ✘
Standard name
Human Ortholog
Description Essential helicase component of heterohexameric MCM2-7 complexes; MCM2-7 complexes bind pre-replication complexes on DNA and melt DNA prior to replication; forms an Mcm4p-6p-7p subcomplex; shows nuclear accumulation in G1; homolog of S. pombe Cdc21p
Localization
Cell Percentages nucleus (84%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-4

Mcm4

Mcm4


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Mcm4-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available