Standard name
Human Ortholog
Description Component of the Rpd3S histone deacetylase complex; Esa1p-associated factor, nonessential component of the NuA4 acetyltransferase complex, homologous to Drosophila dosage compensation protein MSL3; plays a role in regulating Ty1 transposition
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0.06 0 0 0.05 0 0.07 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0.08 0.05 0 0.06 0 0 0.05 0 0.14 0.13 0.17 0 0.07 0.05 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.27 0.06 0.1 0.12 0.14 0.28 0.32 0.21 0.26 0.36 0.34 0.44 0 0 0 0 0 0.08 0 0 0 0 0 0
Nucleus 0.93 0.91 0.9 0.92 0.84 0.81 0.82 0.74 0.77 0.73 0.74 0.73 0.85 0.85 0.79 0.91 0.83 0.89 0.9 0.89 0.78 0.86 0.79 0.69
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.24 0.11 0.13 0.13 0.09 0.17 0.21 0.27 0.3 0.28 0.29 0.28 0 0 0 0.05 0.05 0.05 0.06 0 0.12 0 0.11 0.15
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0.07
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0
Bud 0 0 0 0 0 1 1 3 0 2 4 1 0 0 1 0 0 0 0 0 1 0 1 1
Bud Neck 0 0 0 0 0 0 1 1 1 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 1 7 8 13 10 12 14 8 17 6 0 0 0 0 1 1 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0
Cytoplasm 0 7 3 6 24 11 9 15 11 8 13 7 25 33 50 4 8 4 0 0 0 0 1 1
Endoplasmic Reticulum 0 0 3 0 1 2 0 0 0 0 0 0 0 0 1 0 2 2 0 0 1 0 0 0
Endosome 0 0 0 0 1 0 0 2 0 1 0 0 0 1 0 2 1 0 0 2 1 1 0 1
Golgi 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0
Mitochondria 24 11 23 27 41 63 98 56 70 116 85 118 2 0 2 2 1 7 0 0 4 0 2 2
Nucleus 83 158 201 213 247 183 250 201 209 235 186 196 158 211 230 107 90 78 82 152 179 106 186 137
Nuclear Periphery 0 0 0 2 2 3 5 4 5 2 4 1 1 1 0 1 0 2 0 0 0 0 1 0
Nucleolus 21 20 29 31 26 39 65 72 82 92 72 75 8 7 10 6 5 4 5 4 27 4 24 28
Peroxisomes 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 2 0 0 1 0 2 0 2 0 0 7 3 5 0 3 0 0 2 2 0 3 5
Vac/Vac Membrane 0 3 0 0 4 2 0 4 2 3 0 2 0 2 3 1 5 3 1 3 10 4 9 14
Unique Cell Count 89 174 223 231 294 226 306 271 272 323 250 270 185 249 290 117 109 88 91 171 231 124 235 199
Labelled Cell Count 128 199 262 287 354 319 439 372 394 469 382 406 201 258 302 123 118 101 91 171 231 124 235 199


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.0 6.4 5.8 5.8 5.9 5.2 4.9 5.2 4.9 4.9 5.5 4.7 7.2 7.3 7.0 9.8 10.3 9.8 7.1 7.3 7.9
Std Deviation (1e-4) 0.8 1.2 2.1 2.7 2.7 2.5 1.6 1.7 1.9 1.6 3.4 1.7 2.0 1.6 1.8 3.1 3.1 3.0 1.4 1.5 1.8
Intensity Change (Log2) – – – 0.0 0.02 -0.14 -0.24 -0.15 -0.24 -0.25 -0.07 -0.31 0.31 0.34 0.28 0.76 0.83 0.76 0.3 0.35 0.45


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 3.2 0 2.7 3.0 0 3.6 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 3.5 2.1 1.2 2.5 1.8 0 2.3 0 4.8 4.9 5.9 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.5 1.2 4.7 5.9 3.1 4.4 0 6.1 0 -3.9 -5.2 -5.0 -2.9 -3.1 -0.6
Nucleus 0.8 -2.0 -2.8 -2.7 -4.5 -3.9 -5.0 -4.4 -4.9 -1.5 -1.8 -3.3 0.4 -2.0 -0.4
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.1 -1.5 1.3 2.4 3.7 4.5 0 4.2 0 -3.0 -4.2 -4.0 -2.3 -2.4 -2.2
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 5.2714 4.5358 4.5511 4.3021 3.9605 4.5277 2.9572 3.0906 2.2995 2.265 1.1649 2.1838 1.5766 1.8016 1.2288 1.2018 0.3453 1.0782
Actin 0.0076 0 0.0063 0.0003 0.0007 0.0001 0.0129 0 0.003 0.0032 0.0018 0 0.0109 0 0.0003 0.0167 0.0068 0.0005
Bud 0.0002 0 0.0001 0 0.0003 0 0.0009 0 0.0006 0.0002 0.0029 0.0001 0.0002 0 0.0002 0.0001 0.0001 0.0002
Bud Neck 0.0006 0 0.0003 0.0001 0.0005 0.0004 0.0041 0 0.0003 0.0003 0.0041 0.0007 0.0009 0.0002 0.0002 0.0006 0.0016 0.0011
Bud Periphery 0.0006 0 0.0003 0 0.002 0 0.0014 0 0.0009 0.0006 0.0024 0.0002 0.0001 0 0.0003 0.0001 0.0002 0.0003
Bud Site 0.0019 0 0.0005 0 0.0005 0 0.0042 0 0.0026 0.0004 0.0544 0.0002 0.0009 0.0001 0.0063 0.0001 0.0004 0.0015
Cell Periphery 0.0003 0.0002 0.0001 0 0.0004 0 0.0002 0 0.0001 0.0002 0.0004 0.0001 0.0001 0 0.0001 0 0.0001 0.0001
Cytoplasm 0.0031 0.0056 0.0006 0 0.0003 0 0.0029 0 0.0011 0.0001 0.0026 0.0001 0.0017 0 0.0002 0.0001 0.0022 0.0003
Cytoplasmic Foci 0.0021 0.0001 0.0019 0.0001 0.0043 0.0011 0.0166 0 0.0004 0.0029 0.0309 0.0008 0.006 0 0.0002 0.003 0.0045 0.0011
Eisosomes 0.0004 0 0 0 0 0 0.0002 0 0 0.0001 0 0 0.0001 0 0 0.0001 0.0001 0
Endoplasmic Reticulum 0.0013 0.0006 0.0005 0.0003 0.0002 0 0.0046 0 0.0002 0.0004 0.002 0.0001 0.0011 0 0 0.0001 0.0022 0.0002
Endosome 0.0068 0 0.0026 0.0006 0.0014 0.0002 0.0239 0 0.0009 0.0078 0.0441 0.0018 0.0088 0 0.0002 0.0017 0.0235 0.0021
Golgi 0.0012 0 0.0007 0.0001 0.0004 0.0002 0.01 0 0.0008 0.0097 0.0185 0.0003 0.0017 0 0.0001 0.003 0.0026 0.0004
Lipid Particles 0.0044 0.0001 0.0014 0.001 0.0038 0.0019 0.0228 0 0.0003 0.0424 0.0401 0.0028 0.0019 0 0.0002 0.0045 0.0072 0.0009
Mitochondria 0.0027 0 0.0007 0.0003 0.0088 0.0016 0.0195 0.0001 0.0122 0.0223 0.0196 0.0005 0.0009 0.0001 0.0005 0.0006 0.0022 0.0005
None 0.0019 0.0026 0.0005 0.0001 0.0006 0 0.0016 0 0.0001 0.0001 0.0007 0 0.0019 0 0.0003 0.0001 0.0063 0.0003
Nuclear Periphery 0.0115 0.0001 0.0015 0.0235 0.0034 0.0003 0.0131 0.0002 0.001 0.0021 0.0355 0.0004 0.0061 0.0002 0.0003 0.0002 0.0328 0.0021
Nucleolus 0.0222 0.0188 0.0122 0.0202 0.1408 0.0658 0.0203 0.0175 0.0145 0.017 0.104 0.0536 0.0341 0.0303 0.0192 0.0281 0.126 0.0487
Nucleus 0.9137 0.9712 0.9637 0.9512 0.8025 0.9245 0.7961 0.982 0.9579 0.8844 0.5998 0.9343 0.912 0.9689 0.9707 0.9327 0.7015 0.9305
Peroxisomes 0.0053 0 0.0009 0 0.0013 0.0022 0.0157 0 0.0003 0.0017 0.0161 0.0001 0.0011 0 0.0002 0.0076 0.0012 0.0004
Punctate Nuclear 0.0102 0.0004 0.003 0.0016 0.0223 0.0015 0.0261 0.0001 0.0005 0.0007 0.0069 0.0006 0.0084 0.0002 0.0002 0.0005 0.0753 0.0079
Vacuole 0.0012 0.0001 0.0015 0.0001 0.0034 0.0001 0.0017 0 0.0016 0.0013 0.0097 0.0027 0.0006 0 0.0003 0.0001 0.0014 0.0006
Vacuole Periphery 0.0006 0 0.0006 0.0005 0.0021 0.0001 0.0013 0 0.0007 0.0021 0.0035 0.0007 0.0004 0 0 0.0001 0.0017 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 39.7994 34.3514 37.6284 41.017 41.5186 58.523 48.9979 56.652 57.8327 42.8538
Translational Efficiency 1.2625 1.3582 1.141 1.3497 0.9744 0.8664 0.7537 0.9337 0.8252 0.8676

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
512 157 2817 806 1467 1538 1855 241 1979 1695 4672 1047

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 764.72 846.77 968.26 1075.89 703.75 809.79 953.26 1106.11 719.52 813.22 962.30 1082.85
Standard Deviation 91.15 119.07 122.62 170.98 123.03 110.06 121.02 172.78 118.67 111.44 122.21 171.87
Intensity Change Log 2 — 0.147039 0.340463 0.492527 — 0.202485 0.437807 0.652360 — 0.173877 0.387934 0.571337

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000528 0.001643 0.000685 0.001244 0.000211 0.000750 0.000807 0.001863 0.000293 0.000833 0.000733 0.001387
Bud Neck 0.004688 0.012054 0.031608 0.030156 0.004677 0.008278 0.018151 0.024854 0.004680 0.008628 0.026265 0.028936
Bud Site 0.001092 0.002867 0.001322 0.002920 0.000904 0.001276 0.001269 0.005473 0.000953 0.001423 0.001301 0.003508
Cell Periphery 0.000089 0.000088 0.000052 0.000097 0.000185 0.000097 0.000051 0.001622 0.000160 0.000096 0.000051 0.000448
Cytoplasm 0.002504 0.001091 0.000261 0.000759 0.016398 0.002637 0.000500 0.003096 0.012803 0.002494 0.000356 0.001297
Cytoplasmic Foci 0.001126 0.001458 0.000008 0.001075 0.003265 0.002036 0.000193 0.003086 0.002711 0.001982 0.000082 0.001538
Eisosomes 0.000041 0.000048 0.000052 0.000040 0.000023 0.000038 0.000048 0.000057 0.000028 0.000039 0.000050 0.000044
Endoplasmic Reticulum 0.003798 0.007313 0.002459 0.004125 0.002050 0.005598 0.002832 0.006079 0.002502 0.005757 0.002607 0.004575
Endosome 0.000355 0.000362 0.000139 0.002771 0.000387 0.002357 0.000411 0.004011 0.000379 0.002172 0.000247 0.003056
Golgi 0.000281 0.000290 0.000101 0.001150 0.000223 0.000947 0.000273 0.004967 0.000238 0.000886 0.000169 0.002029
Lipid Particles 0.001495 0.001625 0.000032 0.000216 0.000423 0.001659 0.000093 0.000264 0.000701 0.001656 0.000056 0.000227
Mitochondria 0.001442 0.002575 0.001740 0.004786 0.000704 0.005620 0.001363 0.004598 0.000895 0.005338 0.001591 0.004743
Mitotic Spindle 0.005681 0.001504 0.001711 0.013208 0.000169 0.008901 0.002560 0.017118 0.001595 0.008215 0.002048 0.014108
None 0.003088 0.002510 0.000937 0.001466 0.025183 0.004210 0.002262 0.009451 0.019467 0.004053 0.001463 0.003304
Nuclear Periphery 0.000385 0.000397 0.000353 0.002682 0.000516 0.001845 0.000235 0.001296 0.000482 0.001711 0.000306 0.002363
Nuclear Periphery Foci 0.000403 0.000700 0.000058 0.002177 0.000770 0.001366 0.000279 0.000346 0.000675 0.001304 0.000146 0.001756
Nucleolus 0.043138 0.052492 0.047326 0.031731 0.035031 0.054541 0.060082 0.025427 0.037129 0.054351 0.052391 0.030280
Nucleus 0.927757* 0.907749* 0.910262* 0.888606* 0.907975* 0.890771* 0.906546* 0.872064* 0.913093* 0.892344* 0.908786* 0.884799*
Peroxisomes 0.001388 0.002188 0.000391 0.000747 0.000311 0.001934 0.000872 0.000994 0.000589 0.001957 0.000582 0.000804
Vacuole 0.000565 0.000730 0.000284 0.006561 0.000440 0.003627 0.000599 0.008621 0.000473 0.003359 0.000409 0.007035
Vacuole Periphery 0.000154 0.000317 0.000219 0.003480 0.000154 0.001513 0.000573 0.004714 0.000154 0.001403 0.000360 0.003764

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -4.71 -3.22 -9.13 1.13 -8.57 -8.35 -19.28 -7.93 -5.78 -5.52 -8.54 -16.76 -15.04 -7.71 -9.76
Bud Neck -3.17 -25.48 -14.32 -6.99 -0.70 -5.07 -17.10 -9.55 -7.84 -3.47 -5.77 -32.98 -17.28 -13.96 -3.16
Bud Site -1.46 -1.03 -4.19 0.16 -4.96 -1.78 -2.69 -6.11 -5.60 -5.51 -2.13 -3.21 -8.08 -6.11 -7.22
Cell Periphery -0.14 2.13 -0.19 -0.03 -2.43 4.15 6.88 -0.95 -1.09 -1.16 3.72 7.19 -0.65 -1.07 -1.38
Cytoplasm 1.81 3.02 1.85 0.07 -2.92 7.37 9.01 7.63 0.93 -2.71 7.19 9.38 8.49 2.68 -3.77
Cytoplasmic Foci -0.40 2.30 0.82 0.85 -2.12 1.69 4.08 1.56 0.49 -1.05 1.33 4.55 2.91 1.99 -2.14
Eisosomes -1.34 -2.48 -0.03 1.43 3.97 -7.14 -15.81 -9.23 -5.53 -3.66 -5.48 -14.55 -8.94 -3.08 1.28
Endoplasmic Reticulum -3.91 2.90 -0.79 3.66 -5.28 -7.90 -4.89 -4.61 -0.96 -3.75 -7.78 -1.35 -6.32 1.98 -6.25
Endosome -0.39 3.17 -2.23 -2.14 -2.56 -4.22 0.07 -1.58 0.21 -1.57 -4.24 2.10 -2.67 0.26 -2.91
Golgi -0.06 1.51 -1.29 -1.32 -1.85 -3.15 -0.29 -1.24 -0.85 -1.22 -3.10 0.92 -1.87 -0.63 -2.02
Lipid Particles -0.11 2.41 2.12 1.24 -3.98 -2.59 6.03 1.07 2.77 -2.95 -2.00 3.97 2.80 3.20 -4.57
Mitochondria -2.18 -1.01 -7.42 -3.49 -7.03 -4.79 -6.59 -5.53 1.31 -4.30 -4.75 -4.93 -11.21 0.47 -8.60
Mitotic Spindle 0.62 0.72 -1.81 -2.79 -3.29 -4.91 -3.87 -3.39 -1.95 -2.86 -3.83 -1.95 -4.89 -2.09 -4.32
None 1.36 5.07 3.77 2.39 -3.63 7.96 8.85 5.19 -0.50 -1.30 7.78 9.37 8.41 2.08 -1.58
Nuclear Periphery -0.81 -0.34 -3.29 -2.65 -3.26 -2.70 4.48 -3.02 0.88 -3.92 -2.79 2.66 -3.52 -0.83 -3.76
Nuclear Periphery Foci -1.27 4.22 -2.21 -0.39 -2.86 -1.80 2.05 1.74 3.04 -0.84 -2.27 3.52 -1.50 0.17 -2.83
Nucleolus -1.08 -1.62 2.75 2.70 6.07 -7.42 -9.28 0.00 3.86 5.01 -6.83 -8.59 1.32 7.14 8.45
Nucleus 1.92 3.81 4.83 1.37 2.69 2.78 0.90 3.11 1.78 2.85 3.78 2.48 4.82 1.61 3.80
Peroxisomes -0.98 1.85 1.13 1.97 -4.22 -3.89 -2.56 -5.26 1.27 -1.97 -3.31 0.20 -1.89 2.64 -3.21
Vacuole -1.04 1.73 -4.83 -4.67 -5.07 -4.31 -1.31 -3.29 -1.95 -3.21 -4.29 0.18 -5.93 -2.76 -5.95
Vacuole Periphery -2.46 -2.07 -3.37 -3.13 -3.28 -3.78 -1.39 -1.63 -0.67 -1.35 -3.83 -1.74 -3.74 -1.63 -3.41
Standard name
Human Ortholog
Description Component of the Rpd3S histone deacetylase complex; Esa1p-associated factor, nonessential component of the NuA4 acetyltransferase complex, homologous to Drosophila dosage compensation protein MSL3; plays a role in regulating Ty1 transposition
Localization
Cell Percentages nucleus (99%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-4

Eaf3

Eaf3


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Eaf3-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available