Standard name
Human Ortholog
Description Effector of Rab GTPase Sec4p; forms a complex with Sec4p and t-SNARE Sec9p; involved in exocytosis and docking and fusion of post-Golgi vesicles with plasma membrane; regulates cell proliferation and colony development via the Rho1-Tor1 pathway; homolog of Drosophila lgl tumor suppressor; SRO7 has a paralog, SRO77, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.34 0.18 0.25 0.21 0.16 0.11 – 0.09 0.13 0.09 0.09 0.13 0 0 0 0.06 0.09 0.12 0.23 0.16 0.23 0.16 0.15 0.11
Bud 0.11 0.13 0.08 0.11 0.09 0.06 – 0.11 0.13 0.11 0.15 0.1 0 0 0 0 0.05 0 0.08 0.06 0.07 0.09 0.07 0.06
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0.05 0 0.21 0.17 0.16 0.21 0.12 0.1
Bud Site 0.06 0 0.05 0.05 0 0 – 0 0 0 0.05 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0.22 0.16 0.25 0.32 0.15 0.17 – 0.12 0.16 0.14 0.15 0.15 0.21 0.29 0.23 0.18 0.19 0.23 0.17 0.09 0.24 0.19 0.15 0.16
Cytoplasm 0.5 0.76 0.51 0.6 0.6 0.57 – 0.49 0.41 0.44 0.36 0.31 0.95 0.86 0.92 0.83 0.8 0.74 0.24 0.45 0.16 0.26 0.42 0.47
Endoplasmic Reticulum 0.05 0 0 0 0.05 0 – 0 0 0 0 0.05 0 0 0 0.05 0.05 0.05 0 0 0.06 0 0 0
Endosome 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0.06 0.22 – 0.3 0.43 0.45 0.57 0.53 0 0 0 0.07 0.05 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0.06 0 0 0.08 – 0.06 0.07 0.06 0.05 0.07 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0.09 0 0.12 0.1 0.08 0 – 0.06 0 0 0 0.05 0 0 0 0 0.05 0.07 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 94 26 60 39 56 44 – 59 69 48 45 65 4 9 7 41 52 54 77 27 65 44 52 42
Bud 31 19 19 20 32 25 – 69 71 59 72 53 6 8 4 16 28 17 25 9 19 23 23 22
Bud Neck 1 0 2 1 2 1 – 4 1 3 1 3 2 2 4 15 27 5 71 29 44 56 42 38
Bud Site 16 2 13 9 12 13 – 17 21 13 26 14 0 2 0 8 5 6 – – – – – –
Cell Periphery 59 23 60 61 55 67 – 76 89 72 73 79 30 66 46 123 107 102 59 16 66 52 53 61
Cytoplasm 138 112 123 114 216 222 – 311 224 227 177 160 134 192 186 555 456 320 81 77 46 69 147 186
Endoplasmic Reticulum 15 1 5 7 19 15 – 13 24 10 17 24 1 6 2 35 31 23 9 2 17 8 9 17
Endosome 1 0 1 2 14 17 – 23 12 7 2 5 0 0 0 9 2 7 6 0 3 4 1 3
Golgi 0 1 3 0 1 0 – 0 0 0 0 0 0 0 0 2 1 3 0 1 2 1 2 2
Mitochondria 0 3 6 8 22 88 – 190 233 231 276 271 0 0 0 44 29 14 4 2 7 6 8 9
Nucleus 0 0 0 2 3 2 – 6 3 3 3 3 1 2 1 3 5 1 0 0 0 0 0 0
Nuclear Periphery 0 0 1 0 1 0 – 1 2 4 0 0 0 0 0 0 0 0 0 0 1 0 0 1
Nucleolus 0 0 1 0 1 3 – 5 2 4 3 1 0 0 0 0 0 1 0 0 0 0 0 1
Peroxisomes 6 0 15 7 10 31 – 36 39 32 24 34 0 0 0 0 1 3 0 0 0 0 0 0
SpindlePole 25 0 29 18 29 16 – 38 24 19 17 24 1 4 0 27 30 31 1 0 0 0 0 2
Vac/Vac Membrane 0 0 0 3 9 8 – 15 17 4 7 4 1 0 1 5 2 4 0 2 3 2 1 3
Unique Cell Count 274 147 239 189 359 392 636 547 516 486 513 141 224 203 672 567 435 339 173 282 273 351 398
Labelled Cell Count 386 187 338 291 482 552 863 831 736 743 740 180 291 251 883 776 591 339 173 282 273 351 398


Bud, Cell Periphery, Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 7.3 6.2 6.8 6.9 6.3 5.4 – 5.5 5.0 4.8 4.8 4.8 6.7 7.1 7.3 5.5 6.1 7.5 7.9 7.8 8.0
Std Deviation (1e-4) 1.1 0.8 1.9 2.1 2.1 1.7 – 1.4 1.1 1.2 1.2 1.1 0.9 0.8 1.0 1.0 1.0 1.8 1.5 1.3 1.4
Intensity Change (Log2) – – – 0.02 -0.11 -0.33 – -0.3 -0.43 -0.49 -0.49 -0.48 -0.01 0.07 0.12 -0.29 -0.15 0.15 0.22 0.2 0.24


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches -1.1 -2.9 -4.6 – -6.1 -4.3 0 0 0 -5.6 -6.4 -6.3 -8.0 -6.0 -4.2
Bud 0.9 0.4 -0.8 – 1.3 2.0 0 0 0 -1.4 -2.0 -2.8 -3.8 -1.7 -2.2
Bud Neck 0 0 0 – 0 0 0 0 0 0 0 0 0 2.7 0
Bud Site -0.3 -1.3 -1.3 – -2.0 -1.0 0 0 0 0 -2.8 -3.4 -3.8 -4.0 -3.0
Cell Periphery 1.6 -3.0 -2.4 – -4.8 -2.9 0 0 0 -0.8 1.1 -0.6 -2.3 -2.0 -0.5
Cytoplasm 1.8 2.1 1.3 – -0.7 -2.7 -1.9 -3.9 -5.3 8.8 7.9 9.2 9.5 8.3 5.8
Endoplasmic Reticulum 1.0 2.0 1.2 – 0 1.6 0 0 0 0 0.4 0 2.0 2.1 2.0
Endosome 0 2.7 2.9 – 2.6 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 1.0 2.1 6.8 – 8.7 11.2 0 0 0 0 0 0 2.4 1.7 0.5
Nucleus 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes -1.2 -2.1 0.8 – -0.3 0.4 0 0 0 -3.0 -3.8 -3.6 0 0 -4.3
SpindlePole -0.9 -1.6 -3.8 – -3.1 -4.0 0 0 0 -4.0 -4.3 -5.1 -4.5 -3.4 -2.2
Vacuole 0 0 0 – 0 2.8 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.2321 3.0965 2.1167 2.7818 1.718 2.5488 5.0083 3.5137 3.8924 3.4998 2.9761 4.0017 3.1273 3.6628 3.2078 3.4506 2.4308 3.4742
Actin 0.0903 0.0519 0.0652 0.0467 0.1731 0.0762 0.1225 0.0567 0.0581 0.096 0.2401 0.0499 0.0969 0.0028 0.0927 0.0905 0.1914 0.0702
Bud 0.0063 0.013 0.0487 0.0222 0.0372 0.0032 0.0007 0.0081 0.0401 0.0216 0.0118 0.0012 0.002 0.0104 0.0511 0.021 0.0181 0.0007
Bud Neck 0.0727 0.0066 0.0119 0.0252 0.0457 0.4429 0.0828 0.0076 0.0103 0.0013 0.0078 0.5321 0.0781 0.0025 0.0164 0.023 0.1316 0.4052
Bud Periphery 0.0384 0.0298 0.1581 0.0911 0.1556 0.0174 0.0026 0.0354 0.2687 0.1915 0.0749 0.0082 0.0074 0.0235 0.1793 0.134 0.2922 0.0047
Bud Site 0.0691 0.2627 0.0874 0.0135 0.0235 0.0216 0.0787 0.2844 0.0491 0.0042 0.0405 0.0081 0.0859 0.241 0.0397 0.047 0.0168 0.0148
Cell Periphery 0.072 0.0281 0.031 0.0201 0.0245 0.0325 0.0423 0.0343 0.0507 0.0407 0.1005 0.0282 0.0973 0.0224 0.0474 0.0655 0.0755 0.0302
Cytoplasm 0.5323 0.5144 0.4811 0.5631 0.4764 0.3208 0.4019 0.452 0.3191 0.3951 0.49 0.2681 0.3754 0.5765 0.462 0.5386 0.2276 0.4465
Cytoplasmic Foci 0.0105 0.0067 0.0067 0.0045 0.0069 0.011 0.005 0.0043 0.0106 0.0038 0.0035 0.0069 0.0101 0.0016 0.0101 0.0022 0.0162 0.0053
Eisosomes 0.0017 0.0012 0.0011 0.0004 0.0017 0.0008 0.0021 0.0038 0.0014 0.0022 0.0012 0.0003 0.0013 0.0001 0.0005 0.0003 0.0057 0.0006
Endoplasmic Reticulum 0.0118 0.0054 0.0041 0.0126 0.0041 0.0035 0.0083 0.0052 0.0064 0.0048 0.0052 0.0018 0.0233 0.0021 0.007 0.0017 0.0031 0.0015
Endosome 0.0065 0.0012 0.0027 0.0096 0.0021 0.004 0.0017 0.0011 0.0058 0.0013 0.0026 0.0008 0.0914 0.0003 0.0169 0.0002 0.002 0.0002
Golgi 0.0051 0.0022 0.0029 0.0073 0.005 0.0034 0.0008 0.001 0.0075 0.002 0.0048 0.0011 0.011 0.0003 0.0071 0.0006 0.008 0.0006
Lipid Particles 0.0031 0.0015 0.001 0.0019 0.001 0.0016 0.0019 0.0013 0.0182 0.0027 0.0024 0.0015 0.0034 0.0003 0.0012 0.0003 0.001 0.0002
Mitochondria 0.0012 0.0006 0.0012 0.0013 0.0007 0.0048 0.0001 0.0002 0.0045 0.0009 0.001 0.0001 0.0033 0.0001 0.0023 0.0001 0.0006 0
None 0.0646 0.0693 0.0905 0.1697 0.0378 0.0506 0.2449 0.0981 0.1373 0.2281 0.0061 0.0898 0.076 0.1137 0.0498 0.0726 0.0073 0.0181
Nuclear Periphery 0.0047 0.0004 0.0007 0.0036 0.0007 0.0008 0.0005 0.0003 0.0006 0.0003 0.0003 0.0001 0.0201 0.0002 0.0049 0.0002 0.0003 0.0001
Nucleolus 0.0003 0.0005 0.0004 0.0006 0.0001 0.0002 0.0003 0.0003 0.0003 0.0002 0.0003 0.0001 0.0003 0.0001 0.0007 0.0001 0.0001 0
Nucleus 0.0028 0.0009 0.0009 0.0013 0.0008 0.0006 0.0005 0.0007 0.0006 0.0003 0.0005 0.0002 0.0037 0.0004 0.0017 0.0004 0.0003 0.0002
Peroxisomes 0.0017 0.0003 0.0009 0.0001 0.0006 0.0017 0.0001 0.0001 0.0066 0.0002 0.0005 0.0003 0.0008 0 0.0013 0.0002 0.0012 0.0003
Punctate Nuclear 0.0014 0.0004 0.001 0.0008 0.0004 0.0007 0.0004 0.0003 0.0005 0.0001 0.0001 0.0003 0.001 0.0001 0.0029 0.0002 0.0003 0.0001
Vacuole 0.003 0.0029 0.0021 0.0037 0.0017 0.0014 0.0019 0.0047 0.0032 0.0024 0.0056 0.0008 0.008 0.0016 0.0034 0.0014 0.0007 0.0004
Vacuole Periphery 0.0004 0.0001 0.0002 0.0005 0.0002 0.0002 0.0001 0.0001 0.0004 0.0002 0.0002 0 0.0033 0.0001 0.0019 0.0001 0.0001 0

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 14.584 13.9912 12.9775 18.6151 8.532 11.7318 15.111 16.3833 15.8013 15.0533
Translational Efficiency 0.5592 0.8157 0.9965 0.6755 0.8761 0.7464 0.9129 0.6391 0.7666 0.5946

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Effector of Rab GTPase Sec4p; forms a complex with Sec4p and t-SNARE Sec9p; involved in exocytosis and docking and fusion of post-Golgi vesicles with plasma membrane; regulates cell proliferation and colony development via the Rho1-Tor1 pathway; homolog of Drosophila lgl tumor suppressor; SRO7 has a paralog, SRO77, that arose from the whole genome duplication
Localization
Cell Percentages cytoplasm (50%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-10

Sro7

Sro7


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Sro7-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available