Standard name
Human Ortholog
Description Adapter protein for pexophagy and the Cvt targeting pathway; directs receptor-bound cargo to the phagophore assembly site (PAS) for packaging into vesicles; required for recruiting other proteins to the PAS; recruits Dnm1p to facilitate fission of mitochondria that are destined for removal by mitophagy
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0.05 0 0.05 0.06 0.07 0 0 0 0 – 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0
Cytoplasm 0.76 0.71 0.85 0.88 0.87 0.78 0.76 0.69 0.71 0.63 0.55 0.55 0.81 0.83 0.9 0.67 – 0.65 0.54 0.54 0.51 0.54 0.63 0.57
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 – 0 0 0 0 0 0 0
Endosome 0.06 0 0 0 0 0 0 0 0 0 0 0 0.11 0.1 0 0.11 – 0.21 0.1 0 0.06 0.15 0.09 0.1
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0
Mitochondria 0 0.29 0.07 0.15 0.05 0.21 0.27 0.27 0.36 0.4 0.57 0.57 0 0 0 0.09 – 0 0.11 0.15 0.21 0.05 0.08 0.07
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 – 0 0 0 0 0 0 0
SpindlePole 0.16 0.05 0.08 0.05 0.08 0.08 0.07 0.11 0.06 0.08 0.05 0.08 0.09 0.09 0 0.06 – 0.1 0.15 0.16 0.1 0.17 0.09 0.11
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 – 0.08 0 0 0 0 0 0.05
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 2 0 1 0 0 0 1 0 0 0 1 0 1 0 0 0 – 1 7 2 4 2 2 6
Bud 6 1 4 7 9 9 16 30 7 18 9 20 3 3 7 2 – 1 3 0 0 1 0 2
Bud Neck 6 0 7 4 5 11 10 14 2 3 0 2 0 0 0 1 – 0 4 1 1 1 1 2
Bud Site 1 0 0 2 0 0 3 5 1 2 1 1 0 0 0 0 – 0 – – – – – –
Cell Periphery 1 0 0 0 2 2 2 0 0 0 0 0 0 1 1 2 – 1 1 0 2 0 0 0
Cytoplasm 289 55 149 205 284 266 347 378 175 218 83 148 95 127 190 43 – 41 224 62 98 43 127 129
Endoplasmic Reticulum 2 1 0 1 1 2 1 1 0 0 3 1 0 3 3 5 – 2 3 1 2 0 1 3
Endosome 23 1 0 0 8 1 1 5 0 2 0 0 13 16 8 7 – 13 39 4 10 11 18 21
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 2 0 0 0 0 3
Mitochondria 9 23 12 35 16 72 124 150 90 138 86 154 3 0 8 6 – 1 45 17 40 3 15 16
Nucleus 1 0 0 0 0 1 1 5 1 1 0 2 0 0 0 0 – 0 0 0 0 0 1 0
Nuclear Periphery 0 2 0 0 0 0 1 0 0 0 0 3 0 0 2 0 – 1 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 1 0 0 0 0 1 0 – 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 4 0 2 1 1 4 0 1 6 2 1 2 – 1 3 1 1 0 0 0
SpindlePole 61 4 14 12 25 26 33 59 15 27 7 22 11 14 4 4 – 6 62 18 20 14 18 24
Vac/Vac Membrane 3 1 0 0 2 1 2 3 0 2 0 3 0 0 2 5 – 5 7 3 4 1 8 10
Unique Cell Count 379 78 176 233 328 342 458 546 247 347 152 269 118 153 210 64 63 413 117 194 82 201 227
Labelled Cell Count 404 88 187 266 356 391 544 651 292 416 190 357 132 166 227 77 73 413 117 194 82 201 227


Punctate Nuclear

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.6 3.4 3.1 2.9 3.7 2.9 2.9 3.2 2.6 2.7 2.6 2.7 4.3 4.4 3.9 13.1 – 8.8 4.1 4.1 5.2
Std Deviation (1e-4) 0.8 0.8 0.5 1.0 1.7 0.8 1.2 1.3 1.2 1.0 0.5 1.3 1.1 0.8 1.1 28.1 – 15.9 1.2 1.2 1.6
Intensity Change (Log2) – – – -0.07 0.24 -0.08 -0.11 0.03 -0.25 -0.18 -0.27 -0.22 0.46 0.52 0.32 2.08 – 1.51 0.41 0.42 0.74


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -0.1859 0.405 0.3555 0.1387 -0.091 0.4473 1.9423 1.1231 1.1997 0.8419 1.2079 0.9883 0.3274 0.2492 0.3399 -0.2065 -0.2707 0.1064
Actin 0.0008 0.0132 0.0009 0.0007 0.001 0.0019 0.058 0.011 0.0257 0.0314 0.0297 0.0078 0.1011 0.0025 0.0115 0.0001 0.0002 0.0006
Bud 0 0.0003 0.0002 0.0001 0.0001 0.0004 0.0016 0.0003 0.0025 0.002 0.0011 0.0007 0.0027 0.0003 0.0003 0.0001 0.0001 0
Bud Neck 0.0003 0.0007 0.0012 0.0066 0.0004 0.0102 0.0029 0.0003 0.0047 0.0011 0.0008 0.0101 0.0031 0.0012 0.0049 0.0001 0.0013 0.0088
Bud Periphery 0 0.0003 0.0002 0.0001 0 0.0007 0.0025 0.0006 0.0036 0.002 0.0015 0.0013 0.0128 0.0005 0.0008 0.0001 0.0002 0.0001
Bud Site 0.0003 0.0073 0.0075 0.0006 0.0001 0.0007 0.0216 0.0018 0.0084 0.0041 0.0036 0.0004 0.0172 0.0023 0.0015 0.0002 0.0004 0.0001
Cell Periphery 0.0002 0.0005 0.0002 0.0001 0 0.0002 0.0007 0.0002 0.0003 0.0002 0.0003 0.0002 0.0015 0.0004 0.0007 0 0.0001 0.0001
Cytoplasm 0.1412 0.2465 0.1744 0.1925 0.2539 0.3163 0.0677 0.2307 0.1707 0.312 0.2592 0.3048 0.0604 0.1241 0.0621 0.0707 0.0959 0.0887
Cytoplasmic Foci 0.2882 0.3178 0.3316 0.2083 0.3285 0.2659 0.2194 0.2953 0.3196 0.2145 0.1566 0.1983 0.203 0.1627 0.1618 0.2385 0.3472 0.2689
Eisosomes 0.0003 0.0003 0.0002 0.0005 0 0.0002 0.0004 0.0002 0.0002 0.0001 0.0002 0.0001 0.0009 0.0006 0.0018 0.0001 0.0001 0.0001
Endoplasmic Reticulum 0.0029 0.0032 0.0048 0.0045 0.0022 0.0027 0.0137 0.0034 0.0023 0.0021 0.0016 0.0022 0.0042 0.0055 0.002 0.0009 0.0011 0.0007
Endosome 0.0763 0.069 0.0756 0.0572 0.1163 0.0471 0.1063 0.0926 0.0804 0.0899 0.1637 0.0691 0.0328 0.0221 0.023 0.1554 0.0588 0.0248
Golgi 0.0034 0.0043 0.0042 0.0021 0.0042 0.0024 0.0247 0.0039 0.0142 0.007 0.0571 0.0094 0.006 0.002 0.0023 0.0024 0.0028 0.0014
Lipid Particles 0.0181 0.0156 0.0263 0.0121 0.0093 0.0109 0.046 0.0101 0.0174 0.0484 0.0324 0.0138 0.0261 0.0135 0.0255 0.0114 0.0168 0.0177
Mitochondria 0.0006 0.0008 0.0011 0.0006 0.0004 0.0011 0.0217 0.0006 0.0055 0.0115 0.0665 0.0097 0.0052 0.0015 0.0011 0.0017 0.0082 0.0008
None 0.45 0.2827 0.3269 0.4954 0.2384 0.315 0.2694 0.333 0.2761 0.2007 0.2067 0.3417 0.4922 0.6276 0.6792 0.4823 0.447 0.5724
Nuclear Periphery 0.0005 0.0009 0.001 0.0007 0.0004 0.0004 0.0324 0.0015 0.0041 0.0029 0.0007 0.0011 0.0019 0.0016 0.0007 0.0009 0.0005 0.0002
Nucleolus 0.0002 0.0004 0.0003 0.0002 0.0001 0.0004 0.0031 0.0003 0.0006 0.0024 0.0003 0.0002 0.004 0.001 0.001 0.0003 0.0005 0.0002
Nucleus 0.0005 0.001 0.0008 0.0007 0.0007 0.0007 0.0188 0.001 0.0019 0.0224 0.0005 0.0007 0.0014 0.0009 0.0008 0.0007 0.0004 0.0003
Peroxisomes 0.0103 0.015 0.0265 0.0098 0.0394 0.0153 0.0292 0.0062 0.027 0.0345 0.01 0.0215 0.0139 0.0214 0.0133 0.0177 0.0117 0.0071
Punctate Nuclear 0.0036 0.0165 0.0131 0.0024 0.0014 0.0036 0.0379 0.0034 0.0252 0.0025 0.0014 0.0027 0.0029 0.0053 0.0032 0.0014 0.0021 0.0054
Vacuole 0.0021 0.0035 0.0025 0.004 0.0027 0.0035 0.0144 0.0032 0.0086 0.0066 0.0044 0.0034 0.0057 0.0028 0.0022 0.013 0.0043 0.0016
Vacuole Periphery 0.0003 0.0004 0.0003 0.0007 0.0004 0.0004 0.0076 0.0004 0.001 0.0014 0.0018 0.0009 0.0009 0.0003 0.0002 0.0019 0.0004 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 7.643 6.9718 7.4497 9.6132 6.6738 12.1597 8.9525 10.6574 8.1956 11.1454
Translational Efficiency 0.7879 0.7856 0.5198 0.4966 0.6541 0.4632 0.6462 0.4623 0.658 0.4533

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1404 19 117 164 1833 1872 112 376 3237 1891 229 540

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 574.61 334.57 1087.85 902.24 671.10 710.05 959.32 826.37 629.25 706.28 1024.99 849.41
Standard Deviation 87.14 18.37 98.49 100.13 76.37 96.95 95.37 125.59 94.25 103.49 116.33 123.47
Intensity Change Log 2 — -0.780275 0.920825 0.650928 — 0.081393 0.515484 0.300260 — -0.253990 0.716663 0.472644

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000210 0.000020 0.002537 0.005642 0.000353 0.001000 0.000830 0.002115 0.000291 0.000990 0.001703 0.003186
Bud Neck 0.034673 0.000010 0.009552 0.023411 0.038696 0.051593 0.017423 0.016393 0.036951 0.051074 0.013401 0.018525
Bud Site 0.007368 0.000016 0.026300 0.150138* 0.006186 0.021457 0.010660 0.041283 0.006699 0.021241 0.018651 0.074343
Cell Periphery 0.000276 0.000055 0.000439 0.000561 0.000084 0.000226 0.000283 0.000191 0.000167 0.000224 0.000363 0.000304
Cytoplasm 0.330868* 0.000034 0.166660* 0.203744* 0.309565* 0.244174* 0.169872* 0.325451* 0.318805* 0.241721* 0.168231* 0.288488*
Cytoplasmic Foci 0.338264* 0.000016 0.071844 0.104221 0.377780* 0.289277* 0.105045 0.176327 0.360641* 0.286371* 0.088082 0.154428
Eisosomes 0.000237 0.000049 0.000188 0.000122 0.000182 0.000200 0.000206 0.000102 0.000206 0.000198 0.000197 0.000108
Endoplasmic Reticulum 0.000539 0.000001 0.018703 0.003624 0.000671 0.000745 0.004807 0.002133 0.000614 0.000737 0.011907 0.002586
Endosome 0.045295 0.000002 0.029658 0.058084 0.054804 0.103268 0.040524 0.038658 0.050680 0.102231 0.034972 0.044558
Golgi 0.004555 0.000006 0.000619 0.013709 0.007107 0.015583 0.000610 0.002258 0.006001 0.015427 0.000615 0.005736
Lipid Particles 0.018188 0.000044 0.008389 0.003486 0.015375 0.017385 0.006734 0.003767 0.016595 0.017211 0.007580 0.003682
Mitochondria 0.005151 0.000010 0.000440 0.043349 0.007752 0.021856 0.000346 0.000448 0.006624 0.021636 0.000394 0.013477
Mitotic Spindle 0.000753 0.000483 0.022660 0.035551 0.004273 0.013487 0.001228 0.034413 0.002746 0.013356 0.012178 0.034758
None 0.013307 0.999056* 0.000967 0.001001 0.001396 0.001609 0.001857 0.001409 0.006562 0.011631 0.001402 0.001285
Nuclear Periphery 0.000422 0.000000 0.003741 0.000849 0.000344 0.000504 0.001471 0.001718 0.000377 0.000499 0.002631 0.001454
Nuclear Periphery Foci 0.000664 0.000073 0.026362 0.005917 0.000410 0.000353 0.006149 0.004227 0.000520 0.000350 0.016476 0.004740
Nucleolus 0.004931 0.000046 0.001494 0.001445 0.001508 0.002537 0.007474 0.000938 0.002992 0.002512 0.004419 0.001092
Nucleus 0.110356 0.000012 0.267151* 0.039897 0.081627 0.063475 0.467068* 0.092983 0.094088 0.062837 0.364927* 0.076861
Peroxisomes 0.030413 0.000065 0.028682 0.032398 0.044701 0.039438 0.028243 0.087133 0.038503 0.039043 0.028468 0.070510
Vacuole 0.052636 0.000001 0.313146* 0.270138* 0.046344 0.107421 0.128887 0.167860 0.049073 0.106342 0.223028* 0.198923
Vacuole Periphery 0.000894 0.000000 0.000466 0.002713 0.000841 0.004413 0.000281 0.000192 0.000864 0.004369 0.000376 0.000958

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 6.68 -6.93 -2.80 -3.08 0.11 -2.16 -2.59 -3.16 -1.83 -1.55 -2.38 -6.52 -4.13 -2.18 -0.96
Bud Neck 15.73 8.73 3.63 -4.32 -1.54 -3.98 3.76 6.84 10.28 -0.33 -5.00 7.13 7.66 10.72 -1.24
Bud Site 7.79 -3.46 -4.17 -4.75 -2.97 -7.51 -1.80 -5.80 -2.28 -3.47 -7.33 -3.82 -6.82 -3.72 -4.26
Cell Periphery 2.48 -1.35 0.33 -3.28 2.44 -3.24 -6.73 -5.62 1.34 3.86 -1.06 -4.10 -0.52 0.81 5.16
Cytoplasm 43.59* 12.01 7.21 -9.63 -1.81 6.97 8.23 3.00 -1.02 -4.73 9.35 14.02 6.32 0.50 -5.66
Cytoplasmic Foci 48.86* 26.42 22.12 -8.53 -2.78 11.29 17.91 21.67 14.16 -2.12 11.23 30.12 28.45 18.68 -4.31
Eisosomes 9.42 2.32 6.67 -4.30 4.51 -1.56 -2.06 4.67 5.38 4.68 0.35 -0.13 7.65 6.12 6.17
Endoplasmic Reticulum 5.67 -11.21 -5.81 -6.61 8.41 -0.73 -5.15 -6.88 -6.82 3.39 -1.50 -11.31 -9.04 -8.64 8.77
Endosome 17.85 4.76 2.35 -5.05 -0.56 -10.04 1.14 5.51 13.98 1.35 -11.95 3.03 5.72 14.72 0.81
Golgi 10.09 8.82 0.83 -2.35 -1.97 -6.19 8.75 6.76 11.29 -0.82 -7.53 11.77 5.81 10.53 -2.09
Lipid Particles 14.42 6.78 9.41 -10.70 3.45 -1.39 3.77 8.69 11.34 2.99 -0.60 7.21 12.74 11.56 4.45
Mitochondria 7.64 6.85 -0.89 -1.85 -1.75 -7.60 7.63 7.56 13.32 -0.50 -8.85 9.95 1.84 8.00 -1.75
Mitotic Spindle 1.29 -1.62 -3.58 -3.62 -1.83 -4.42 -0.72 -5.05 -3.83 -2.97 -5.53 -1.62 -6.25 -4.44 -3.52
None -487.87* 6.39 5.06 1029.31* -1.82 -0.88 -0.74 -1.61 -0.61 -0.08 -2.01 5.69 4.86 4.10 -1.57
Nuclear Periphery 11.02 -9.46 -8.13 -9.96 4.85 -2.57 -6.25 -3.22 -2.99 -0.61 -2.21 -10.78 -4.37 -4.11 1.84
Nuclear Periphery Foci 9.40 -5.46 -4.63 -4.93 3.46 1.44 -3.13 -6.24 -6.30 1.32 3.89 -6.17 -7.74 -7.92 3.64
Nucleolus 9.73 5.69 3.83 -2.91 -0.52 -2.91 -2.08 -1.10 0.45 1.76 2.71 -0.41 2.45 0.48 1.46
Nucleus 25.75 -7.05 5.02 -9.21 8.55 4.30 -9.27 -1.92 -3.95 8.57 8.10 -11.57 0.71 -3.73 11.39
Peroxisomes 15.91 2.86 0.67 -4.00 -1.35 1.56 2.33 -0.12 -0.87 -1.97 -0.21 3.77 -0.18 -0.06 -2.90
Vacuole 20.68 -15.15 -16.27 -18.48 -2.21 -14.74 -7.08 -17.24 -12.96 -4.08 -14.41 -14.82 -22.86 -18.03 -2.77
Vacuole Periphery 9.93 4.24 0.38 -2.35 -0.85 -11.60 4.94 7.88 14.22 2.51 -11.70 6.46 4.36 12.94 0.52
Standard name
Human Ortholog
Description Adapter protein for pexophagy and the Cvt targeting pathway; directs receptor-bound cargo to the phagophore assembly site (PAS) for packaging into vesicles; required for recruiting other proteins to the PAS; recruits Dnm1p to facilitate fission of mitochondria that are destined for removal by mitophagy
Localization
Cell Percentages —
Cell Cycle Regulation No
Subcompartmental Group N/A

Atg11

Atg11


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Atg11-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available