Standard name
Human Ortholog
Description Membrane protein; has similarity to mammalian mannose-6-phosphate receptors; possibly functions as a sorting receptor in the delivery of vacuolar hydrolases; protein abundance increases in response to DNA replication stress
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.05 0 0 0.08 0.09 0.15 0.17 0.14 0.21 0.26 0.25 0.33 0 0 0 0.06 0.1 0.05 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0
Endosome 0.19 0.26 0.34 0.4 0.37 0.07 0.12 0 0 0.11 0 0 0.46 0.58 0.57 0.43 0.39 0.42 0.43 0.53 0.56 0.65 0.55 0.55
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.17 0.05 0.48 0.46 0.4 0.62 0.59 0.6 0.58 0.43 0.62 0.58 0 0.05 0 0.07 0.1 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0
Nucleolus 0.2 0.17 0.2 0.16 0.07 0 0 0 0 0 0 0 0.23 0.23 0.38 0.16 0.16 0.18 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0.06 0.08 0 0 0 0 0 0 0 0 0.05 0.11 0.1 0.15 0.1 0.12 0 0 0 0 0 0
Vac/Vac Membrane 0.67 0.73 0.39 0.34 0.44 0.29 0.34 0.35 0.34 0.4 0.32 0.26 0.67 0.49 0.43 0.62 0.61 0.65 0.43 0.38 0.29 0.24 0.32 0.34
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 1 0 0 0 0 3 3 2 5 1 1 2 1 0 0 1 0 0 1 1 1 0 0 2
Bud 0 0 4 5 15 19 31 21 23 20 12 24 0 2 1 0 0 1 1 2 0 0 3 1
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0
Bud Site 0 0 0 0 0 0 1 0 1 1 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 3 3 8 5 6 15 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 13 14 17 32 57 108 158 135 161 169 123 295 6 11 10 13 26 16 1 1 1 2 2 2
Endoplasmic Reticulum 6 1 1 0 4 35 10 19 28 16 21 27 3 8 5 6 8 17 1 1 4 0 1 2
Endosome 55 93 150 165 238 48 110 39 28 70 7 20 134 226 214 96 106 124 137 197 261 174 225 231
Golgi 2 0 0 4 5 10 1 0 1 0 0 1 2 8 11 3 8 5 2 6 3 2 8 5
Mitochondria 50 19 209 192 255 459 538 569 436 274 303 518 7 20 8 16 27 10 8 3 17 6 11 6
Nucleus 9 6 2 1 10 10 17 12 13 8 3 6 2 2 2 3 3 7 5 1 1 1 4 3
Nuclear Periphery 7 4 22 10 6 14 16 30 14 9 5 7 12 17 16 4 3 4 10 8 23 7 8 11
Nucleolus 57 61 88 65 43 18 20 34 17 6 1 3 67 91 143 35 43 53 5 3 2 2 1 5
Peroxisomes 0 0 0 1 2 3 1 0 0 0 0 0 0 5 0 1 1 0 1 0 0 0 0 0
SpindlePole 6 5 27 35 26 8 10 1 5 10 1 0 15 42 36 33 28 36 3 5 6 3 3 2
Vac/Vac Membrane 193 256 172 140 282 213 308 330 256 253 156 237 197 189 162 139 167 193 138 141 137 65 131 146
Unique Cell Count 287 352 437 415 640 738 914 943 753 638 492 900 293 388 374 224 272 297 322 376 469 270 408 425
Labelled Cell Count 399 459 692 650 943 948 1227 1195 996 842 639 1155 446 621 608 350 420 466 322 376 469 270 408 425


Endosome

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 8.5 9.5 6.0 5.9 5.8 5.5 5.2 5.4 5.6 5.4 5.4 5.4 9.4 9.6 11.4 9.5 9.5 9.2 9.9 10.1 11.1
Std Deviation (1e-4) 2.3 2.2 1.4 1.4 1.5 1.3 1.1 1.2 1.3 1.2 1.3 1.2 1.9 2.7 2.2 2.5 2.6 2.3 2.3 2.6 2.8
Intensity Change (Log2) – – – -0.0 -0.04 -0.11 -0.19 -0.14 -0.08 -0.15 -0.14 -0.16 0.66 0.69 0.93 0.67 0.67 0.62 0.74 0.76 0.9


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 1.7 2.0 2.7 1.7 2.4 2.4 1.8 2.1 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 2.4 3.2 5.8 6.9 5.8 8.2 9.6 9.0 11.7 -1.4 -0.8 -1.0 1.1 3.1 1.0
Endoplasmic Reticulum 0 0 4.3 0 2.6 3.8 2.9 4.0 3.3 0 0 0 0 0 4.7
Endosome 1.6 1.0 -12.3 -9.7 -15.2 -14.3 -9.3 -13.4 -16.5 3.1 6.9 6.5 2.1 1.3 2.0
Golgi 0 0 0 0 0 0 0 0 0 0 0 3.6 0 0 0
Mitochondria -0.5 -2.6 4.8 3.8 4.4 3.4 -1.6 4.2 3.4 -13.2 -13.7 -14.7 -10.4 -10.4 -12.9
Nucleus 0 0 0 2.0 0 1.9 0 0 0 0 0 0 0 0 0
Nuclear Periphery -2.0 -4.1 -3.0 -3.4 -1.7 -3.1 -3.5 -3.6 -5.0 -0.6 -0.4 -0.5 -2.0 -2.8 -2.7
Nucleolus -1.7 -6.6 -10.2 -11.4 -10.1 -10.5 -10.9 -10.3 -13.5 0.9 1.2 5.7 -1.4 -1.4 -0.8
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 1.3 -1.6 -5.0 -5.4 -7.4 -5.7 -4.1 -5.3 -7.5 -0.6 2.4 1.8 3.6 2.0 2.8
Vacuole -1.7 1.5 -3.7 -2.0 -1.6 -1.9 0.1 -2.4 -4.8 7.4 2.7 1.1 5.5 5.7 6.8