Standard name
Human Ortholog
Description RNA polymerase subunit AC40; common to RNA polymerase I and III; predominant determinant targeting Ty1 integration upstream of Pol III-transcribed genes
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0.05 0 0 0 0 0 0.05 0 0.05 0 – 0.06 0 0.08 0 0 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.48 0.58 0.37 0.22 0.13 0.35 0.42 0.35 0.39 0.32 0.45 0.25 – 0.2 0.16 0.06 0.08 0.06 0 0 0 0 0 0.06
Nucleus 0.22 0.26 0.11 0.2 0.15 0.07 0.1 0.1 0.07 0.09 0.1 0.07 – 0.16 0.12 0.11 0.12 0.15 0.17 0.16 0.07 0 0.05 0.05
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.89 0.95 0.89 0.8 0.83 0.88 0.89 0.9 0.87 0.84 0.86 0.92 – 0.79 0.87 0.82 0.86 0.85 0.76 0.74 0.81 0.76 0.72 0.65
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0.05 0 0.06 0.11 0.16
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 1 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 1 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 1 0 1 – 0 0 0 0 0 0 0 0 0 0 0
Bud Site 1 0 0 0 0 0 1 1 4 1 3 2 – 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 1 2 1 2 6 3 4 7 10 7 5 1 – 6 5 13 9 2 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 2 2 2 0 1 0 0 0 0
Endosome 0 0 0 0 0 1 1 0 0 0 0 0 – 0 0 0 0 0 3 0 0 0 0 0
Golgi 0 0 0 0 1 0 0 0 0 0 0 0 – 0 0 2 0 0 0 0 0 0 2 1
Mitochondria 173 38 7 12 25 44 85 77 71 68 48 21 – 19 18 10 20 9 0 0 1 0 3 8
Nucleus 80 17 2 11 29 9 21 23 13 20 11 6 – 15 13 19 28 24 61 12 3 0 3 7
Nuclear Periphery 1 0 0 0 0 0 0 0 1 0 1 0 – 0 0 0 0 0 1 0 0 0 1 1
Nucleolus 317 62 17 44 159 110 182 200 160 178 91 76 – 76 97 137 206 131 275 58 39 34 61 91
Peroxisomes 0 0 0 1 0 1 1 0 0 0 0 0 – 0 0 1 0 0 0 0 0 0 1 0
SpindlePole 0 0 0 0 0 0 2 5 3 4 3 0 – 0 0 0 0 0 2 0 0 0 0 2
Vac/Vac Membrane 0 0 0 0 2 0 2 1 1 2 0 0 – 1 0 3 4 1 10 3 1 2 9 22
Unique Cell Count 358 65 19 55 192 125 204 222 184 213 106 83 96 112 168 239 155 361 78 49 46 86 141
Labelled Cell Count 573 119 27 70 222 168 299 314 263 281 162 107 117 133 187 271 169 361 78 49 46 86 141


Nucleolus, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 31.4 36.1 22.8 29.0 27.3 19.5 19.6 19.2 17.7 17.3 17.6 14.8 – 37.2 31.9 67.1 65.0 66.6 28.8 30.7 36.4
Std Deviation (1e-4) 11.6 13.3 8.4 8.5 11.3 7.0 8.0 7.2 8.8 7.5 10.8 5.8 – 17.3 12.6 23.6 24.3 21.1 11.7 10.1 12.2
Intensity Change (Log2) – – – 0.35 0.26 -0.23 -0.22 -0.25 -0.36 -0.4 -0.38 -0.62 – 0.71 0.49 1.56 1.51 1.55 0.34 0.43 0.67


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0
Mitochondria 0 0 -0.1 0.4 -0.2 0.1 -0.4 0.7 -1.0 – 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0
Nucleolus -0.9 -0.7 -0.2 0 0.1 -0.3 -0.7 -0.4 0.3 – -1.0 -0.3 -0.9 -0.4 -0.6
Peroxisomes 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 32.0383 32.7368 27.0745 29.5685 23.319 28.2919 32.6208 35.1135 30.048 28.4014 19.946 31.3376 40.4431 37.0366 34.8027 33.2075 20.1479 34.9982
Actin 0.0003 0 0.0072 0 0.001 0.0005 0.0267 0 0.013 0.0007 0.0228 0.0059 0.0118 0 0.0105 0.0002 0.0578 0.0012
Bud 0 0 0.0001 0.0014 0 0.0001 0.0002 0 0.0003 0.0009 0.0002 0.0003 0.0001 0 0.0001 0.0001 0.005 0.0001
Bud Neck 0.0001 0 0.0001 0.0001 0 0.0001 0.0006 0 0.0004 0.0003 0.0006 0.0005 0.0001 0 0.0001 0.0001 0.0005 0.0001
Bud Periphery 0 0 0.0001 0.0007 0 0.0001 0.0003 0 0.0003 0.0007 0.0003 0.0006 0.0001 0 0.0001 0.0001 0.0152 0.0001
Bud Site 0.0005 0 0.0009 0.0001 0.0001 0 0.001 0 0.0023 0.0087 0.0007 0.0005 0.0003 0 0.0006 0.0014 0.0054 0.0003
Cell Periphery 0.0001 0 0.0001 0.0014 0.0001 0.0001 0.0002 0.0002 0.0002 0.0003 0.0001 0.0001 0.0001 0.0001 0.0002 0.0002 0.0008 0.0001
Cytoplasm 0.0014 0 0.0042 0.0005 0.0046 0.0001 0.0114 0 0.0218 0.0047 0.012 0.0003 0.0024 0 0.0052 0.0013 0.0004 0.0001
Cytoplasmic Foci 0.012 0 0.0114 0.0001 0.0093 0.002 0.0162 0 0.0238 0.068 0.0489 0.0005 0.0093 0 0.0163 0.0137 0.0121 0.0044
Eisosomes 0 0 0.0001 0 0.0001 0 0.0005 0 0.0001 0.0001 0.0002 0.0001 0.0001 0 0.0001 0.0001 0.0005 0
Endoplasmic Reticulum 0.0001 0 0.0004 0 0.0049 0 0.0014 0 0.0041 0.0007 0.0075 0.0018 0.0012 0 0.0005 0.0002 0.0019 0.0001
Endosome 0.0032 0 0.0039 0 0.0236 0.0004 0.0101 0 0.0167 0.033 0.0411 0.0056 0.0102 0 0.0079 0.0052 0.0199 0.0048
Golgi 0.0005 0 0.0015 0 0.0018 0.0006 0.0032 0 0.0065 0.0071 0.0052 0.0021 0.0066 0 0.0015 0.001 0.0231 0.0038
Lipid Particles 0.0034 0 0.0043 0 0.0045 0.0064 0.006 0.0001 0.0048 0.0164 0.0205 0.0009 0.0074 0.0002 0.0051 0.0062 0.0277 0.0097
Mitochondria 0.001 0 0.0074 0.0009 0.0141 0.0047 0.0024 0.0003 0.0088 0.0056 0.0081 0.0013 0.0024 0.0002 0.003 0.0035 0.1336 0.0028
None 0.0015 0 0.0021 0.0001 0.0007 0.0003 0.008 0 0.0034 0.0052 0.0179 0.0005 0.0022 0 0.001 0.0032 0.0011 0.0001
Nuclear Periphery 0.0005 0 0.0051 0.0001 0.004 0.0001 0.0163 0.0001 0.0059 0.0017 0.07 0.0109 0.0064 0 0.001 0.0027 0.0055 0.0002
Nucleolus 0.8588 0.799 0.7574 0.7715 0.8453 0.8558 0.7459 0.7735 0.732 0.676 0.5548 0.8421 0.8048 0.8174 0.7652 0.7359 0.5758 0.8407
Nucleus 0.1067 0.2008 0.1864 0.2148 0.0824 0.1254 0.1246 0.2249 0.1394 0.1496 0.1111 0.1204 0.1274 0.1813 0.1751 0.1879 0.0569 0.1245
Peroxisomes 0.0014 0 0.0013 0 0.0004 0.0017 0.0111 0 0.0089 0.0026 0.0334 0.0004 0.0026 0 0.0032 0.0016 0.0449 0.0055
Punctate Nuclear 0.0079 0.0001 0.0048 0.0001 0.0017 0.0003 0.0128 0.0001 0.0056 0.0097 0.0396 0.0043 0.0032 0.0002 0.0018 0.0324 0.0036 0.001
Vacuole 0.0003 0 0.0003 0.0053 0.0006 0.0004 0.0006 0.0003 0.0009 0.0058 0.0018 0.0003 0.0005 0.0003 0.0006 0.0011 0.0035 0.0002
Vacuole Periphery 0.0002 0 0.0007 0.0027 0.0009 0.0008 0.0008 0.0005 0.0009 0.0022 0.0033 0.0006 0.001 0.0001 0.0009 0.0019 0.0048 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 155.9872 175.1601 157.5691 149.5327 134.8295 99.5578 143.959 114.8589 118.1679 142.5824
Translational Efficiency 1.4831 1.3591 1.3565 1.2074 1.3796 1.7658 1.2873 1.2992 1.244 1.3144

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
2154 1404 700 1865 1518 2069 2213 731 3672 3473 2913 2596

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 2388.41 2501.24 3182.62 3887.75 2291.93 2770.91 3368.08 1901.67 2348.53 2661.89 3323.51 3328.50
Standard Deviation 758.16 850.31 1005.96 1004.16 703.77 831.71 818.25 606.45 737.70 849.65 870.69 1275.12
Intensity Change Log 2 — 0.066593 0.414164 0.702885 — 0.273797 0.555363 -0.269296 — 0.171778 0.485036 0.306805

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000353 0.000356 0.000448 0.000536 0.000356 0.000363 0.000411 0.000488 0.000354 0.000360 0.000420 0.000523
Bud Neck 0.000738 0.000885 0.001309 0.003767 0.000938 0.001240 0.002721 0.005018 0.000821 0.001097 0.002382 0.004119
Bud Site 0.000559 0.000262 0.000170 0.000880 0.000305 0.000340 0.000468 0.000999 0.000454 0.000309 0.000396 0.000913
Cell Periphery 0.000024 0.000017 0.000013 0.000074 0.000071 0.000028 0.000021 0.000085 0.000043 0.000024 0.000019 0.000077
Cytoplasm 0.001638 0.000198 0.000017 0.000380 0.000505 0.000120 0.000097 0.001330 0.001169 0.000151 0.000078 0.000648
Cytoplasmic Foci 0.004010 0.004900 0.000499 0.002993 0.002886 0.002846 0.001523 0.002075 0.003545 0.003676 0.001277 0.002734
Eisosomes 0.000343 0.000224 0.000236 0.000140 0.000257 0.000284 0.000198 0.000078 0.000308 0.000260 0.000207 0.000122
Endoplasmic Reticulum 0.000080 0.000059 0.000062 0.000100 0.000076 0.000083 0.000085 0.000290 0.000078 0.000073 0.000079 0.000153
Endosome 0.000007 0.000006 0.000003 0.000333 0.000028 0.000007 0.000012 0.001202 0.000016 0.000007 0.000010 0.000577
Golgi 0.000057 0.000056 0.000040 0.000164 0.000159 0.000051 0.000100 0.000504 0.000099 0.000053 0.000085 0.000260
Lipid Particles 0.000009 0.000005 0.000002 0.000021 0.000016 0.000007 0.000004 0.000041 0.000012 0.000007 0.000003 0.000027
Mitochondria 0.000657 0.000736 0.000863 0.001445 0.000619 0.000739 0.001069 0.001806 0.000642 0.000738 0.001019 0.001547
Mitotic Spindle 0.000043 0.000047 0.000131 0.003216 0.000240 0.000654 0.001286 0.003802 0.000125 0.000409 0.001008 0.003381
None 0.001004 0.001143 0.000348 0.000709 0.000961 0.000822 0.000446 0.002230 0.000986 0.000952 0.000422 0.001138
Nuclear Periphery 0.000020 0.000015 0.000028 0.000142 0.000019 0.000019 0.000047 0.000289 0.000020 0.000017 0.000042 0.000183
Nuclear Periphery Foci 0.000037 0.000032 0.000012 0.000073 0.000087 0.000026 0.000017 0.000331 0.000058 0.000028 0.000016 0.000146
Nucleolus 0.423338* 0.397492* 0.359883* 0.545972* 0.458581* 0.397186* 0.505137* 0.519369* 0.437907* 0.397310* 0.470232* 0.538481*
Nucleus 0.517429* 0.525638* 0.606075* 0.408472* 0.455653* 0.532136* 0.439634* 0.441298* 0.491891* 0.529509* 0.479630* 0.417716*
Peroxisomes 0.048827 0.067131 0.028855 0.029052 0.077626 0.062417 0.046043 0.013646 0.060732 0.064323 0.041913 0.024714
Vacuole 0.000359 0.000307 0.000226 0.000592 0.000294 0.000260 0.000176 0.002851 0.000332 0.000279 0.000188 0.001228
Vacuole Periphery 0.000469 0.000492 0.000782 0.000938 0.000322 0.000371 0.000506 0.002269 0.000408 0.000420 0.000572 0.001312

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -0.18 -3.94 -8.62 -8.48 -3.75 -0.21 -2.16 -5.86 -7.49 -5.50 -0.40 -4.50 -9.88 -10.53 -6.91
Bud Neck -1.82 -5.20 -13.01 -12.30 -10.90 -3.36 -13.34 -14.28 -13.04 -8.73 -4.17 -14.51 -18.04 -16.33 -10.06
Bud Site 1.58 2.16 -1.69 -3.82 -4.42 -0.47 -2.18 -4.51 -4.50 -3.79 1.30 0.58 -3.25 -5.02 -4.45
Cell Periphery 1.73 2.72 -3.49 -4.12 -4.41 1.06 1.21 -0.18 -3.25 -3.83 1.17 1.42 -1.61 -4.63 -5.11
Cytoplasm 2.09 2.41 1.77 -1.13 -2.67 1.71 1.80 -1.31 -2.28 -2.32 2.48 2.69 1.20 -2.71 -3.22
Cytoplasmic Foci -0.84 5.11 0.31 1.05 -3.97 -0.00 2.73 0.26 0.24 -1.10 -0.30 4.52 0.20 0.43 -3.06
Eisosomes 7.76 5.99 13.93 6.23 5.95 -1.75 5.13 16.76 16.66 15.58 4.23 10.25 17.98 13.19 9.54
Endoplasmic Reticulum 2.40 2.01 -2.98 -6.51 -5.81 -0.33 -0.65 -8.44 -8.23 -8.84 0.50 -0.20 -8.11 -8.26 -9.49
Endosome 0.91 2.98 -1.76 -1.77 -1.79 0.93 0.72 -2.10 -2.16 -2.15 0.99 0.64 -2.71 -2.78 -2.76
Golgi 0.27 2.56 -7.04 -7.41 -8.71 1.09 0.66 -1.49 -2.70 -2.37 1.13 0.45 -2.43 -4.76 -4.03
Lipid Particles 1.26 2.79 -1.74 -2.20 -2.65 0.69 0.98 -1.23 -2.39 -2.70 0.92 1.52 -1.81 -2.98 -3.49
Mitochondria -1.39 -2.05 -10.52 -10.11 -6.05 -2.25 -8.28 -3.30 -2.94 -1.97 -2.34 -7.68 -8.31 -7.41 -4.74
Mitotic Spindle -0.33 -1.78 -4.93 -4.93 -4.83 -1.47 -3.23 -2.59 -2.25 -1.63 -1.75 -3.61 -5.53 -5.05 -3.90
None -0.67 5.93 1.80 2.11 -4.05 0.71 3.51 -2.70 -2.99 -3.94 0.27 5.92 -1.01 -1.16 -4.89
Nuclear Periphery 0.66 -0.85 -4.64 -4.85 -4.52 0.11 -5.87 -4.26 -4.27 -3.81 0.50 -4.26 -6.23 -6.35 -5.55
Nuclear Periphery Foci 0.38 2.54 -2.07 -2.23 -2.91 1.07 1.21 -1.61 -2.38 -2.45 1.19 1.67 -2.16 -3.31 -3.62
Nucleolus 2.84 5.49 -13.38 -14.82 -15.14 6.83 -5.28 -2.45 -6.86 0.84 6.42 -4.83 -12.15 -17.35 -7.30
Nucleus -0.80 -7.08 11.64 11.02 15.43 -7.52 1.79 -0.61 4.69 -1.89 -5.27 1.81 8.68 13.13 6.56
Peroxisomes -6.13 7.57 10.13 13.09 -0.40 4.73 10.30 23.38 23.44 19.23* -1.72 9.71 21.50 22.00 11.20
Vacuole 0.51 2.01 -1.06 -1.23 -1.65 0.41 1.37 -2.17 -2.23 -2.31 0.79 2.83 -2.42 -2.59 -2.90
Vacuole Periphery -0.40 -3.12 -5.30 -4.89 -1.29 -1.44 -7.50 -2.12 -2.06 -1.90 -0.36 -4.68 -3.56 -3.50 -2.87
Standard name
Human Ortholog
Description RNA polymerase subunit AC40; common to RNA polymerase I and III; predominant determinant targeting Ty1 integration upstream of Pol III-transcribed genes
Localization
Cell Percentages nucleolus (57%)
Cell Cycle Regulation No
Subcompartmental Group nucleolus-2

Rpc40

Rpc40


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Rpc40-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available