Standard name
Human Ortholog
Description Ammonium permease of high capacity and low affinity; belongs to Mep-Amt-Rh family of well-conserved ammonium (NH4+) transporters that includes the human Rh factors; expression is under the nitrogen catabolite repression regulation ammonia permease activity regulated by TORC1 effectors, Npr1p and Par32p; MEP3 has a paralog, MEP1, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.33 0.28 0.31 0.34 0.4 0.31 0.22 0.18 0.14 0.07 0.1 0.06 0.29 0.31 0.27 0 0.07 0 0 0 0 0.06 0 0
Bud 0 0.05 0 0 0.07 0.09 0.1 0.1 0.12 0.06 0.07 0.08 0.06 0.06 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0.91 0.84 0.86 0.81 0.72 0.52 0.44 0.27 0.29 0.27 0.23 0.17 0.82 0.82 0.76 0.56 0.42 0.12 0.93 0.81 0.92 0.8 0.9 0.87
Cytoplasm 0.05 0.13 0.05 0.09 0.1 0.37 0.45 0.58 0.58 0.71 0.7 0.74 0.15 0.15 0.2 0.44 0.42 0.75 0 0.06 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0.11 0.13 0.12 0.1 0.09 0.09 0.07 0.07 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.06 0 0.21 0.1 0 0 0 0 0 0 0 0 0 0 0 0 0.09 0.06 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.09 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0.08 0.05 0.06 0.06 0.11 0 0 0 0 0 0.06 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 57 56 96 72 106 66 62 56 36 23 26 22 81 86 87 0 3 0 2 7 10 21 16 12
Bud 2 10 9 8 19 19 30 31 29 18 18 28 17 17 11 0 0 0 0 3 1 3 3 6
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 3 1 3 3 9
Bud Site 0 0 0 1 0 0 1 2 3 1 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 159 169 265 171 191 110 128 84 74 85 56 59 229 229 246 9 19 2 194 192 311 279 459 435
Cytoplasm 8 27 16 19 27 77 131 176 146 227 174 258 42 42 63 7 19 12 2 15 0 8 3 6
Endoplasmic Reticulum 1 6 8 6 29 27 35 31 23 28 18 26 7 7 13 0 0 0 4 2 7 13 11 10
Endosome 0 0 2 0 1 0 0 7 3 2 1 2 0 0 0 0 0 0 0 0 0 0 0 1
Golgi 0 0 0 1 2 0 0 1 0 0 0 0 0 0 1 0 0 0 0 0 0 1 0 0
Mitochondria 10 8 64 21 4 4 6 4 5 7 6 6 3 3 4 0 4 1 0 3 2 8 6 8
Nucleus 1 1 0 0 0 0 3 3 6 9 5 5 1 1 0 0 4 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 1 0 2 0 0 0 0 0 0 0 0 0 0 0 1 0 1
Nucleolus 0 0 0 0 0 0 0 2 0 1 0 2 0 0 0 0 0 0 0 0 0 0 0 1
Peroxisomes 0 0 1 6 7 5 5 3 1 0 0 1 0 0 1 0 0 0 0 0 0 0 0 0
SpindlePole 1 0 1 0 2 0 2 2 2 0 1 0 2 1 5 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 2 0 0 3 4 2 25 13 19 15 40 2 3 3 0 1 1 0 4 0 3 1 3
Unique Cell Count 175 202 308 211 265 210 288 306 251 318 248 350 280 279 322 16 45 16 208 236 339 348 508 501
Labelled Cell Count 239 279 462 305 391 312 405 428 341 422 320 449 384 389 434 16 50 16 208 236 339 348 508 501


Cell Periphery

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 14.9 14.5 14.0 14.2 12.6 10.4 10.3 10.6 10.3 9.7 9.7 9.7 12.2 12.2 12.1 12.7 13.7 14.8 13.6 14.4 15.1
Std Deviation (1e-4) 2.4 3.1 2.8 2.6 3.0 3.4 2.8 2.8 2.7 2.6 2.4 2.2 2.3 2.3 2.4 3.5 4.4 3.9 2.8 2.7 3.0
Intensity Change (Log2) – – – 0.02 -0.15 -0.43 -0.44 -0.4 -0.45 -0.52 -0.52 -0.53 -0.19 -0.19 -0.2 -0.14 -0.03 0.08 -0.04 0.04 0.11


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0.7 2.2 0.1 -2.7 -3.7 -4.7 -7.6 -5.9 -8.3 -0.6 -0.1 -1.1 0 -3.4 0
Bud 0.5 2.4 3.0 3.7 3.6 4.0 1.7 2.4 2.8 1.9 1.9 0.4 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery -1.5 -4.1 -8.4 -10.7 -14.7 -13.6 -14.9 -15.1 -17.7 -1.4 -1.3 -3.1 -3.2 -6.9 -7.5
Cytoplasm 1.7 2.3 9.2 11.4 14.0 13.7 17.0 16.1 17.8 4.0 4.0 5.4 0 0 0
Endoplasmic Reticulum 0.2 4.1 4.6 4.5 3.8 3.4 3.3 2.6 2.8 -0.1 -0.1 1.0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria -3.3 -7.1 -6.2 -7.1 -7.7 -6.7 -7.3 -6.5 -7.9 -7.5 -7.5 -7.9 0 -1.9 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 5.1 4.0 4.4 4.4 6.1 0 0 0 0 0 0