| ORF | |
|---|---|
| Human Ortholog | |
| Description | Bifunctional triacylglycerol lipase and short chain ester hydrolase; null mutant results in the accumulation of both triacylglycerol and fatty acids derived from neutral lipids and phospholipids as well as an increase in the quantity of lipid droplets; contains an alpha/beta hydrolase domain with a conserved GXSXG lipase motif; localizes to lipid droplets; GFP-fusion protein localizes to the cytoplasm and is induced in response to the DNA-damaging agent MMS |
Micrographs
Sub-cellular Localization
Yeast GFP Assignment
Protein Abundance
Localization Change
External localization resources
| ensLOC | DeepLoc | |||||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
| Cortical Patches | 0 | 1 | 0 | 0 | 0 | – | 0 | 2 | 0 | 1 | 0 | 0 | 1 | 1 | 0 | – | – | 0 | 2 | 11 | 2 | 2 | 4 | 6 |
| Bud | 1 | 2 | 0 | 0 | 1 | – | 0 | 1 | 1 | 1 | 2 | 2 | 2 | 0 | 0 | – | – | 0 | 0 | 2 | 0 | 1 | 9 | 6 |
| Bud Neck | 0 | 3 | 1 | 1 | 2 | – | 0 | 4 | 3 | 1 | 2 | 2 | 3 | 3 | 0 | – | – | 2 | 1 | 3 | 1 | 2 | 2 | 1 |
| Bud Site | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | – | – | 0 | – | – | – | – | – | – |
| Cell Periphery | 0 | 3 | 8 | 0 | 0 | – | 0 | 0 | 1 | 0 | 0 | 1 | 2 | 0 | 1 | – | – | 3 | 1 | 3 | 3 | 1 | 2 | 2 |
| Cytoplasm | 160 | 484 | 123 | 39 | 54 | – | 34 | 31 | 26 | 21 | 27 | 48 | 233 | 405 | 491 | – | – | 121 | 149 | 389 | 103 | 226 | 219 | 252 |
| Endoplasmic Reticulum | 4 | 7 | 2 | 0 | 0 | – | 0 | 4 | 0 | 2 | 3 | 3 | 7 | 9 | 0 | – | – | 13 | 3 | 2 | 2 | 1 | 1 | 1 |
| Endosome | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 52 | 9 | 4 | – | – | 5 | 3 | 8 | 1 | 3 | 3 | 8 |
| Golgi | 1 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | – | – | 0 | 1 | 1 | 1 | 0 | 0 | 2 |
| Mitochondria | 3 | 15 | 0 | 2 | 5 | – | 11 | 4 | 23 | 16 | 54 | 58 | 2 | 0 | 0 | – | – | 6 | 18 | 46 | 19 | 16 | 16 | 29 |
| Nucleus | 0 | 3 | 0 | 0 | 2 | – | 1 | 0 | 0 | 1 | 0 | 2 | 1 | 1 | 1 | – | – | 4 | 0 | 1 | 0 | 0 | 0 | 1 |
| Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 1 | – | – | 0 | 0 | 1 | 0 | 1 | 1 | 1 |
| Nucleolus | 0 | 0 | 0 | 1 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| Peroxisomes | 0 | 1 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 4 | 0 | 0 | – | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| SpindlePole | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 3 | 0 | 1 | 2 | 28 | 4 | 0 | – | – | 2 | 0 | 6 | 1 | 2 | 3 | 9 |
| Vac/Vac Membrane | 0 | 3 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 1 | 6 | 2 | 4 | – | – | 8 | 5 | 5 | 0 | 4 | 5 | 7 |
| Unique Cell Count | 167 | 500 | 126 | 40 | 56 | 40 | 42 | 43 | 35 | 81 | 97 | 318 | 425 | 497 | 141 | 191 | 495 | 139 | 271 | 278 | 342 | |||
| Labelled Cell Count | 169 | 522 | 134 | 43 | 64 | 46 | 46 | 57 | 43 | 89 | 120 | 342 | 437 | 502 | 164 | 191 | 495 | 139 | 271 | 278 | 342 | |||
Yeast GFP Assignment
Protein Abundance
| Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Mean Cell GFP Intensity (1e-4) | 4.0 | 3.5 | 4.8 | 4.5 | 3.4 | – | 3.4 | 3.8 | 3.1 | 3.2 | 3.1 | 3.3 | 5.1 | 4.6 | 4.4 | 4.2 | 4.9 | 5.1 | 5.1 | 5.6 | 5.3 |
| Std Deviation (1e-4) | 0.6 | 0.7 | 0.9 | 1.5 | 0.6 | – | 0.6 | 0.6 | 1.1 | 0.5 | 0.4 | 0.7 | 1.4 | 1.0 | 1.5 | 1.3 | 1.2 | 1.2 | 1.7 | 2.2 | 1.6 |
| Intensity Change (Log2) | – | – | – | -0.1 | -0.49 | – | -0.5 | -0.34 | -0.63 | -0.6 | -0.63 | -0.55 | 0.1 | -0.07 | -0.13 | -0.21 | 0.02 | 0.09 | 0.08 | 0.22 | 0.14 |
Localization Change
| Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Bud | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Endosome | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Golgi | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
External localization resources
