| ORF | |
|---|---|
| Human Ortholog | |
| Description | Protein of unknown function; may interact with ribosomes, based on co-purification experiments; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern |
Micrographs
Sub-cellular Localization
Yeast GFP Assignment
Protein Abundance
Localization Change
External localization resources
| ensLOC | DeepLoc | |||||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
| Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | – | 0 | 2 | 0 | 6 | 2 | 3 | 5 |
| Bud | 0 | 0 | 2 | 1 | 1 | 8 | 5 | 3 | 5 | 6 | 9 | 4 | 1 | 0 | 0 | 0 | – | 0 | 1 | 1 | 4 | 6 | 6 | 10 |
| Bud Neck | 0 | 1 | 1 | 0 | 2 | 3 | 2 | 4 | 2 | 2 | 2 | 4 | 2 | 2 | 0 | 1 | – | 1 | 1 | 1 | 3 | 0 | 3 | 2 |
| Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 3 | 1 | 0 | 0 | 0 | 0 | – | 0 | – | – | – | – | – | – |
| Cell Periphery | 2 | 1 | 3 | 2 | 0 | 4 | 1 | 2 | 1 | 1 | 1 | 3 | 4 | 3 | 2 | 3 | – | 3 | 0 | 0 | 5 | 1 | 3 | 1 |
| Cytoplasm | 93 | 240 | 268 | 170 | 240 | 217 | 287 | 340 | 294 | 332 | 240 | 293 | 296 | 278 | 265 | 196 | – | 240 | 59 | 223 | 182 | 167 | 299 | 308 |
| Endoplasmic Reticulum | 0 | 2 | 1 | 1 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 1 | 1 | 10 | – | 13 | 4 | 0 | 3 | 0 | 1 | 2 |
| Endosome | 0 | 0 | 5 | 1 | 2 | 1 | 2 | 4 | 3 | 0 | 2 | 1 | 1 | 0 | 0 | 2 | – | 1 | 1 | 2 | 6 | 1 | 2 | 4 |
| Golgi | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | – | 1 | 1 | 0 | 4 | 0 | 0 | 2 |
| Mitochondria | 9 | 5 | 5 | 8 | 9 | 49 | 80 | 83 | 104 | 157 | 158 | 174 | 0 | 0 | 1 | 3 | – | 4 | 21 | 9 | 64 | 7 | 15 | 8 |
| Nucleus | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 4 | 2 | 0 | 3 | 2 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 3 | 1 | 0 |
| Nuclear Periphery | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 1 | 0 | 0 | 0 | 1 | 0 | 1 |
| Nucleolus | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 1 | 0 | – | 0 | 0 | 0 | 1 | 0 | 0 | 0 |
| Peroxisomes | 0 | 0 | 1 | 1 | 0 | 0 | 1 | 2 | 2 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | – | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
| SpindlePole | 1 | 0 | 3 | 0 | 4 | 5 | 6 | 11 | 9 | 2 | 6 | 14 | 0 | 0 | 0 | 0 | – | 2 | 0 | 0 | 3 | 4 | 8 | 7 |
| Vac/Vac Membrane | 0 | 1 | 3 | 0 | 1 | 4 | 1 | 5 | 4 | 3 | 2 | 2 | 1 | 0 | 0 | 0 | – | 3 | 3 | 1 | 3 | 4 | 5 | 5 |
| Unique Cell Count | 100 | 244 | 282 | 173 | 251 | 249 | 327 | 397 | 361 | 401 | 343 | 409 | 299 | 279 | 268 | 202 | 249 | 105 | 244 | 298 | 207 | 358 | 372 | |
| Labelled Cell Count | 106 | 251 | 294 | 184 | 261 | 293 | 386 | 459 | 427 | 505 | 427 | 501 | 308 | 284 | 270 | 216 | 270 | 105 | 244 | 298 | 207 | 358 | 372 | |
Yeast GFP Assignment
Protein Abundance
| Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Mean Cell GFP Intensity (1e-4) | 2.9 | 3.7 | 3.8 | 3.7 | 3.9 | 3.0 | 3.1 | 3.1 | 2.8 | 2.8 | 2.5 | 2.5 | 5.1 | 5.0 | 4.9 | 5.6 | 4.8 | 5.3 | 5.4 | 5.5 | 6.0 |
| Std Deviation (1e-4) | 0.6 | 0.6 | 1.5 | 2.0 | 2.3 | 2.0 | 1.9 | 1.7 | 0.6 | 1.7 | 1.1 | 0.9 | 0.9 | 1.2 | 1.1 | 1.9 | 1.6 | 2.2 | 2.0 | 1.7 | 2.6 |
| Intensity Change (Log2) | – | – | – | -0.05 | 0.04 | -0.34 | -0.3 | -0.31 | -0.42 | -0.42 | -0.64 | -0.6 | 0.41 | 0.38 | 0.36 | 0.55 | 0.34 | 0.47 | 0.51 | 0.52 | 0.65 |
Localization Change
| Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Bud | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Endosome | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Golgi | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
External localization resources
