Standard name
Human Ortholog
Description Subunit of the RNA polymerase II mediator complex; conserved from yeast to human; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; required for transcriptional activation and has a role in basal transcription; protein abundance increases in response to DNA replication stress
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0.06 0.05 – 0.05 0 0.06 0 0 0.09 0.13 0.18 0.08 0.14 0.13 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0.05 0.05 0.05 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0.15 0.19 0 0.14 – 0.18 0.32 0.28 0.35 0.32 0 0 0 0.05 0.09 0 0 0 0 0 0 0
Nucleus 0.87 0.86 0.87 0.64 0.74 0.64 – 0.64 0.56 0.64 0.66 0.65 0.75 0.78 0.67 0.54 0.49 0.55 0.76 0.65 0.73 0.57 0.55 0.54
Nuclear Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.17 0.24 0.16 0.25 0.11 0.3 – 0.34 0.4 0.34 0.37 0.29 0.2 0.19 0.21 0.14 0.18 0.13 0.15 0.28 0.21 0.29 0.3 0.27
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.05 0.05 0 0 0.12 0.05 – 0 0 0 0 0 0.06 0 0.05 0.24 0.18 0.17 0 0 0 0 0.05 0.08
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 1 – 0 0 0 0 0 1 0 0 0 1 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 – 2 1 2 2 0 0 0 0 0 0 0 9 1 1 6 5 11
Bud Neck 0 0 0 0 0 0 – 0 0 3 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 1 1 0 1 – 2 3 8 7 10 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0
Cytoplasm 6 3 2 3 5 11 – 14 6 12 3 8 21 30 50 16 25 20 0 0 0 1 3 3
Endoplasmic Reticulum 0 0 0 0 0 0 – 1 0 0 0 2 2 0 2 10 3 6 0 0 0 0 2 1
Endosome 1 0 1 0 1 3 – 1 0 0 0 0 3 1 3 11 9 8 6 0 0 1 6 11
Golgi 0 0 0 0 0 0 – 0 0 0 0 1 0 1 0 2 2 0 3 2 0 0 1 0
Mitochondria 5 1 16 14 3 29 – 47 56 52 85 74 1 2 1 10 16 3 2 0 0 0 1 1
Nucleus 453 118 96 46 61 136 – 164 97 121 158 150 171 176 187 111 86 82 458 107 86 133 181 187
Nuclear Periphery 0 0 0 0 0 1 – 1 0 1 0 0 0 0 0 1 0 0 0 0 0 0 0 0
Nucleolus 90 33 18 18 9 63 – 88 69 63 89 68 46 42 58 29 31 20 91 45 25 66 97 93
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 – 0 0 0 0 0 0 0 1 0 2 0 7 2 0 10 6 6
Vac/Vac Membrane 27 7 3 2 10 10 – 6 5 3 0 6 13 6 13 49 32 26 19 3 3 10 16 26
Unique Cell Count 518 138 110 72 82 213 257 174 188 241 231 228 226 281 206 176 150 600 164 119 234 328 348
Labelled Cell Count 582 162 137 84 89 255 326 237 265 344 319 258 258 315 239 207 166 600 164 119 234 328 348


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 11.4 15.2 7.9 10.4 11.4 8.5 – 7.8 6.9 7.6 7.0 7.1 11.9 11.7 11.4 14.3 13.1 13.2 10.9 11.9 12.3
Std Deviation (1e-4) 2.1 2.7 1.8 2.2 2.8 1.9 – 1.7 1.9 2.2 1.6 1.9 2.6 2.4 2.7 4.6 4.2 3.8 2.2 2.7 2.8
Intensity Change (Log2) – – – 0.39 0.53 0.1 – -0.02 -0.19 -0.06 -0.18 -0.15 0.59 0.56 0.53 0.86 0.73 0.74 0.47 0.59 0.64


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 – 0 0 1.8 0 0 2.5 3.4 4.2 2.2 3.5 3.3
Endoplasmic Reticulum 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.9 -2.5 -0.2 – 0.9 3.3 2.6 4.0 3.4 -5.6 -5.2 0 -3.0 -1.4 -3.8
Nucleus -3.7 -2.3 -4.4 – -4.5 -5.5 -4.3 -4.2 -4.3 -2.6 -2.1 -4.1 -5.9 -6.6 -5.6
Nuclear Periphery 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 1.4 -1.1 2.6 – 3.5 4.1 3.2 3.9 2.6 0.8 0.5 1.0 -0.5 0.3 -0.7
Peroxisomes 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 2.6 0 – 0 0 0 0 0 1.2 0 0 4.8 3.9 3.7

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 6.6127 7.7737 7.1502 6.4152 4.8767 6.8009 5.6265 7.5467 6.6669 6.743 5.0205 6.8153 7.5007 8.8287 8.5934 8.2078 7.7071 8.2277
Actin 0.0075 0 0.0008 0.0007 0.0361 0.0088 0.0104 0 0.0134 0 0.0065 0 0.0065 0 0.0014 0.0021 0.0009 0.0051
Bud 0.0002 0 0.0001 0.0001 0.0004 0.0001 0.0006 0 0.0005 0 0.0005 0.0001 0.0007 0.0001 0.0002 0.0001 0.0001 0.0001
Bud Neck 0.0005 0.0002 0.0002 0.0004 0.0016 0.0017 0.0022 0.0002 0.0009 0.0004 0.0011 0.0015 0.0009 0.0003 0.0001 0.0002 0.0008 0.0028
Bud Periphery 0.0005 0 0.0001 0.0001 0.0006 0.0001 0.001 0 0.0009 0 0.0009 0.0002 0.0011 0 0.0001 0.0001 0.0001 0.0001
Bud Site 0.0004 0.0002 0.0003 0.0008 0.0013 0.0002 0.0028 0.0001 0.0023 0.0001 0.0019 0.0002 0.0014 0.0002 0.0001 0.0002 0.0002 0.0002
Cell Periphery 0.0001 0 0 0 0.0002 0 0.0004 0 0.0005 0 0.0002 0 0.0001 0 0 0 0 0
Cytoplasm 0.0038 0.0002 0.0099 0.0084 0.0023 0.0008 0.0131 0.0005 0.0033 0.0005 0.0026 0.0002 0.0075 0.0034 0.0017 0.0005 0.0024 0.0027
Cytoplasmic Foci 0.0065 0 0.0007 0.0141 0.0136 0.0016 0.0326 0 0.0101 0.017 0.0394 0 0.0152 0 0.0004 0.0132 0.0029 0.0005
Eisosomes 0 0 0 0 0.0005 0 0.0001 0 0.0002 0 0.0003 0 0.0001 0 0 0 0 0
Endoplasmic Reticulum 0.0021 0 0.0001 0.0028 0.0021 0.0008 0.006 0 0.0041 0.0001 0.002 0 0.002 0 0.0008 0.0001 0.002 0.0027
Endosome 0.0085 0 0.0004 0.0181 0.0217 0.0026 0.0282 0 0.0141 0.0081 0.049 0.0001 0.0224 0 0.0021 0.0045 0.013 0.0032
Golgi 0.0021 0 0.0005 0.0015 0.0243 0.0012 0.0036 0 0.004 0.0025 0.0334 0 0.0034 0 0.0002 0.0047 0.0008 0.0009
Lipid Particles 0.0026 0 0.0012 0.0038 0.0539 0.0047 0.0156 0 0.0063 0.0246 0.0288 0 0.009 0 0.0001 0.0052 0.0044 0.0004
Mitochondria 0.0016 0.0001 0.0025 0.0004 0.0144 0.0005 0.0023 0.0001 0.0035 0.0004 0.029 0.0001 0.0049 0.0001 0.0004 0.0007 0.0003 0.0005
None 0.0014 0 0.0001 0.002 0.0168 0.0005 0.0064 0 0.0006 0 0.0082 0 0.0026 0.0001 0.0003 0.0001 0.0006 0.0002
Nuclear Periphery 0.0122 0.0031 0.0028 0.0043 0.0424 0.0025 0.0238 0.0012 0.009 0.001 0.0377 0.0006 0.0368 0.002 0.0036 0.0012 0.0181 0.0111
Nucleolus 0.0096 0.0232 0.0151 0.0051 0.0196 0.025 0.0177 0.0086 0.0083 0.0052 0.0287 0.0257 0.0102 0.0089 0.0066 0.0061 0.0109 0.0367
Nucleus 0.9244 0.9702 0.9604 0.9198 0.6838 0.9421 0.7809 0.9878 0.9023 0.9372 0.6809 0.9695 0.8459 0.9732 0.9805 0.9477 0.9215 0.9273
Peroxisomes 0.0031 0 0.0029 0.0005 0.0135 0.0005 0.0106 0 0.0063 0.0008 0.0353 0 0.0063 0 0.0001 0.0102 0.0004 0.0001
Punctate Nuclear 0.0106 0.0025 0.0016 0.0151 0.0469 0.0056 0.0317 0.0014 0.0037 0.001 0.0054 0.0013 0.0192 0.0113 0.0013 0.003 0.0195 0.0046
Vacuole 0.0016 0.0001 0.0001 0.0015 0.0014 0.0004 0.0084 0.0001 0.0048 0.0007 0.0038 0.0002 0.0019 0.0001 0.0001 0.0001 0.0008 0.0008
Vacuole Periphery 0.0008 0 0.0001 0.0004 0.0026 0.0002 0.0017 0 0.0009 0.0002 0.0044 0 0.0017 0 0.0001 0 0.0004 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 15.1311 12.4858 13.8975 15.999 19.6181 9.3063 13.6764 13.4909 14.8256 14.7275
Translational Efficiency 1.321 1.3984 1.5687 0.9811 0.8014 2.6177 1.5344 1.3758 1.6457 1.3197

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Subunit of the RNA polymerase II mediator complex; conserved from yeast to human; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; required for transcriptional activation and has a role in basal transcription; protein abundance increases in response to DNA replication stress
Localization
Cell Percentages nucleus (99%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-4

Nut2

Nut2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Nut2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available