Standard name | |
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Human Ortholog | |
Description | Adapter that links synaptojanins to the cortical actin cytoskeleton; the synaptojanins are Inp52p and Inp53p |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 122 | 31 | 86 | 77 | 123 | 59 | 69 | – | 42 | 51 | 31 | 46 | 30 | 87 | 89 | 125 | 138 | 132 | 209 | 111 | 113 | 36 | 61 | 98 |
Bud | 40 | 20 | 13 | 26 | 54 | 19 | 45 | – | 26 | 35 | 34 | 22 | 9 | 21 | 15 | 39 | 34 | 36 | 15 | 5 | 10 | 2 | 12 | 17 |
Bud Neck | 0 | 0 | 1 | 1 | 1 | 0 | 1 | – | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 3 | 6 | 1 | 5 | 3 | 11 | 4 | 9 | 4 |
Bud Site | 20 | 2 | 1 | 6 | 8 | 4 | 12 | – | 8 | 9 | 3 | 6 | 2 | 2 | 0 | 1 | 8 | 1 | – | – | – | – | – | – |
Cell Periphery | 2 | 3 | 17 | 4 | 9 | 1 | 5 | – | 0 | 5 | 2 | 16 | 5 | 10 | 21 | 35 | 24 | 32 | 0 | 1 | 0 | 0 | 0 | 0 |
Cytoplasm | 34 | 11 | 26 | 18 | 35 | 27 | 41 | – | 30 | 22 | 21 | 32 | 10 | 33 | 35 | 62 | 70 | 79 | 11 | 5 | 9 | 0 | 3 | 2 |
Endoplasmic Reticulum | 5 | 5 | 12 | 12 | 15 | 15 | 18 | – | 8 | 7 | 18 | 21 | 6 | 7 | 10 | 16 | 12 | 20 | 2 | 1 | 1 | 0 | 1 | 1 |
Endosome | 14 | 9 | 6 | 2 | 9 | 12 | 33 | – | 6 | 15 | 1 | 7 | 2 | 9 | 5 | 31 | 23 | 13 | 14 | 31 | 12 | 2 | 19 | 16 |
Golgi | 36 | 10 | 43 | 10 | 27 | 19 | 13 | – | 14 | 3 | 3 | 14 | 11 | 36 | 52 | 28 | 36 | 47 | 19 | 10 | 13 | 3 | 1 | 5 |
Mitochondria | 164 | 79 | 19 | 37 | 20 | 76 | 130 | – | 171 | 126 | 194 | 176 | 4 | 7 | 6 | 30 | 17 | 22 | 8 | 23 | 14 | 2 | 7 | 10 |
Nucleus | 2 | 0 | 0 | 0 | 1 | 4 | 3 | – | 4 | 1 | 0 | 1 | 0 | 1 | 1 | 2 | 4 | 3 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 2 | 1 | 0 | 0 | 1 | 1 | 1 | – | 0 | 0 | 0 | 5 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 3 | 0 | 0 | 0 | 0 |
Nucleolus | 1 | 4 | 0 | 1 | 1 | 0 | 3 | – | 1 | 2 | 2 | 3 | 0 | 0 | 0 | 3 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 59 | 8 | 17 | 25 | 51 | 41 | 41 | – | 17 | 17 | 8 | 11 | 6 | 35 | 19 | 35 | 20 | 40 | 27 | 12 | 25 | 4 | 7 | 7 |
SpindlePole | 7 | 2 | 6 | 2 | 25 | 12 | 23 | – | 10 | 22 | 7 | 4 | 1 | 8 | 5 | 80 | 49 | 76 | 4 | 4 | 1 | 0 | 1 | 1 |
Vac/Vac Membrane | 3 | 11 | 8 | 1 | 4 | 1 | 4 | – | 0 | 0 | 1 | 2 | 0 | 0 | 0 | 17 | 10 | 11 | 0 | 3 | 1 | 0 | 1 | 1 |
Unique Cell Count | 287 | 135 | 156 | 136 | 224 | 193 | 287 | 237 | 218 | 242 | 254 | 53 | 158 | 167 | 337 | 318 | 342 | 322 | 221 | 216 | 58 | 132 | 169 | |
Labelled Cell Count | 511 | 196 | 255 | 222 | 384 | 291 | 442 | 337 | 317 | 325 | 366 | 87 | 256 | 258 | 507 | 452 | 513 | 322 | 221 | 216 | 58 | 132 | 169 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 7.6 | 8.7 | 8.6 | 7.7 | 8.0 | 6.7 | 6.5 | – | 5.7 | 5.8 | 5.1 | 5.6 | 8.9 | 9.2 | 8.5 | 9.2 | 8.7 | 8.7 | 7.5 | 8.8 | 8.8 |
Std Deviation (1e-4) | 1.5 | 2.0 | 1.7 | 1.3 | 1.9 | 1.4 | 1.4 | – | 1.2 | 1.4 | 1.2 | 1.3 | 1.5 | 1.6 | 1.6 | 5.5 | 1.4 | 1.4 | 0.9 | 1.4 | 1.5 |
Intensity Change (Log2) | – | – | – | -0.16 | -0.11 | -0.36 | -0.4 | – | -0.59 | -0.56 | -0.75 | -0.62 | 0.05 | 0.1 | -0.01 | 0.1 | 0.02 | 0.02 | -0.2 | 0.04 | 0.04 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0.3 | 0 | -4.6 | -6.6 | – | -7.7 | -6.3 | -9.0 | -7.8 | 0.2 | 0 | -0.3 | -3.8 | -2.4 | -3.4 |
Bud | 2.7 | 4.0 | 0.5 | 2.2 | – | 0.9 | 2.2 | 1.7 | 0.1 | 1.8 | 1.4 | 0.2 | 1.1 | 0.8 | 0.8 |
Bud Neck | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | -2.6 | -2.6 | -4.4 | -4.2 | – | -5.2 | -3.5 | -4.6 | -1.7 | -0.3 | -1.4 | 0.5 | -0.2 | -1.2 | -0.5 |
Cytoplasm | -0.8 | -0.3 | -0.7 | -0.7 | – | -1.1 | -1.9 | -2.4 | -1.1 | 0.4 | 1.0 | 1.0 | 0.5 | 1.4 | 1.6 |
Endoplasmic Reticulum | 0.4 | -0.4 | 0 | -0.6 | – | -1.9 | -1.9 | -0.1 | 0.2 | 0 | -1.2 | -0.6 | -1.3 | -1.8 | -0.8 |
Endosome | 0 | 0.1 | 1.0 | 2.7 | – | 0 | 1.3 | 0 | 0 | 0 | 0.8 | -0.4 | 2.1 | 1.4 | 0 |
Golgi | -4.5 | -3.8 | -4.3 | -7.0 | – | -6.0 | -7.6 | -8.0 | -6.3 | -1.0 | -1.0 | 0.7 | -5.7 | -4.5 | -3.7 |
Mitochondria | 3.3 | -1.0 | 5.7 | 7.0 | – | 11.6 | 8.9 | 13.3 | 11.2 | -0.9 | -2.5 | -2.9 | -1.1 | -2.6 | -2.2 |
Nucleus | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 1.8 | 3.0 | 2.6 | 1.0 | – | -1.3 | -1.0 | -3.0 | -2.6 | 0.1 | 2.7 | 0.1 | -0.2 | -1.8 | 0.3 |
SpindlePole | 0 | 2.6 | 1.0 | 1.7 | – | 0.2 | 2.3 | -0.5 | 0 | 0 | 0.5 | -0.4 | 5.4 | 3.7 | 5.1 |
Vacuole | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | -1.1 | -1.0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 2.9187 | 2.8324 | 2.8842 | 2.5431 | 2.4146 | 3.026 | 4.0785 | 3.2078 | 3.3662 | 3.2795 | 3.3239 | 3.5107 | 4.0564 | 3.0539 | 3.191 | 3.1134 | 3.1737 | 3.2548 |
Actin | 0.6503 | 0.531 | 0.7404 | 0.6937 | 0.839 | 0.7566 | 0.7123 | 0.631 | 0.7944 | 0.7222 | 0.8727 | 0.7606 | 0.7554 | 0.6827 | 0.7841 | 0.6553 | 0.6342 | 0.7467 |
Bud | 0.0015 | 0.0123 | 0.0321 | 0.0221 | 0.0055 | 0.0019 | 0.0022 | 0.011 | 0.0186 | 0.0254 | 0.0135 | 0.0019 | 0.0017 | 0.014 | 0.0395 | 0.0522 | 0.0094 | 0.0012 |
Bud Neck | 0.0046 | 0.0024 | 0.0017 | 0.0008 | 0.0064 | 0.0771 | 0.0037 | 0.0031 | 0.0056 | 0.0013 | 0.0152 | 0.085 | 0.0042 | 0.0061 | 0.0058 | 0.0023 | 0.0059 | 0.1184 |
Bud Periphery | 0.0017 | 0.0127 | 0.0531 | 0.0664 | 0.0128 | 0.003 | 0.0034 | 0.0142 | 0.0248 | 0.0539 | 0.0291 | 0.0029 | 0.0017 | 0.0091 | 0.0444 | 0.0705 | 0.0081 | 0.002 |
Bud Site | 0.0131 | 0.1238 | 0.0258 | 0.0058 | 0.0052 | 0.0026 | 0.0153 | 0.1173 | 0.0322 | 0.0079 | 0.0058 | 0.0038 | 0.0162 | 0.0931 | 0.0244 | 0.0489 | 0.0328 | 0.0035 |
Cell Periphery | 0.0003 | 0.0009 | 0.0011 | 0.0005 | 0.0008 | 0.0002 | 0.0003 | 0.0017 | 0.0004 | 0.0005 | 0.0004 | 0.0003 | 0.0004 | 0.0008 | 0.0005 | 0.0015 | 0.0008 | 0.0004 |
Cytoplasm | 0.0044 | 0.0612 | 0.0177 | 0.0068 | 0.0193 | 0.0036 | 0.0051 | 0.0377 | 0.0037 | 0.003 | 0.0013 | 0.0065 | 0.0125 | 0.0497 | 0.0205 | 0.0097 | 0.0367 | 0.0037 |
Cytoplasmic Foci | 0.0603 | 0.078 | 0.0241 | 0.0355 | 0.0104 | 0.0269 | 0.0421 | 0.0393 | 0.02 | 0.0152 | 0.0177 | 0.0291 | 0.0401 | 0.0529 | 0.021 | 0.0296 | 0.0666 | 0.0344 |
Eisosomes | 0.0006 | 0.0004 | 0.0006 | 0.0005 | 0.0005 | 0.0007 | 0.0007 | 0.0013 | 0.0004 | 0.0003 | 0.0004 | 0.0006 | 0.0018 | 0.0005 | 0.0003 | 0.001 | 0.0005 | 0.0006 |
Endoplasmic Reticulum | 0.0014 | 0.0008 | 0.0005 | 0.0022 | 0.0002 | 0.0002 | 0.0011 | 0.0011 | 0.0005 | 0.0004 | 0.0011 | 0.0002 | 0.0034 | 0.0007 | 0.0004 | 0.0025 | 0.0045 | 0.001 |
Endosome | 0.0362 | 0.02 | 0.0055 | 0.0299 | 0.0073 | 0.0145 | 0.0268 | 0.0074 | 0.0081 | 0.0222 | 0.0116 | 0.0052 | 0.0192 | 0.012 | 0.0034 | 0.0511 | 0.0584 | 0.0069 |
Golgi | 0.049 | 0.0345 | 0.0143 | 0.0412 | 0.0215 | 0.0342 | 0.0631 | 0.0334 | 0.0127 | 0.0326 | 0.0049 | 0.016 | 0.0247 | 0.0121 | 0.0101 | 0.0147 | 0.0552 | 0.0126 |
Lipid Particles | 0.0199 | 0.0027 | 0.0039 | 0.0124 | 0.01 | 0.0034 | 0.0067 | 0.0019 | 0.0021 | 0.0016 | 0.0039 | 0.004 | 0.0098 | 0.0013 | 0.0017 | 0.0029 | 0.004 | 0.005 |
Mitochondria | 0.0154 | 0.0104 | 0.0167 | 0.0219 | 0.0209 | 0.0109 | 0.0133 | 0.0051 | 0.0061 | 0.022 | 0.0039 | 0.0053 | 0.0172 | 0.0037 | 0.0086 | 0.0113 | 0.0094 | 0.0031 |
None | 0.0029 | 0.0176 | 0.0035 | 0.0096 | 0.0025 | 0.005 | 0.0279 | 0.0327 | 0.0106 | 0.0004 | 0.0006 | 0.006 | 0.0256 | 0.0043 | 0.0068 | 0.0024 | 0.0195 | 0.0121 |
Nuclear Periphery | 0.0011 | 0.0003 | 0.0002 | 0.0031 | 0.0005 | 0.0013 | 0.0028 | 0.0005 | 0.0004 | 0.0002 | 0.0039 | 0.0009 | 0.0174 | 0.0003 | 0.0002 | 0.0011 | 0.0024 | 0.0014 |
Nucleolus | 0.003 | 0.0002 | 0.0001 | 0.0003 | 0.0001 | 0.0001 | 0.0005 | 0.0005 | 0.0003 | 0.0001 | 0.0006 | 0.0002 | 0.0016 | 0.0003 | 0.0001 | 0.0006 | 0.0013 | 0.0001 |
Nucleus | 0.002 | 0.0005 | 0.0002 | 0.0004 | 0.0001 | 0.0002 | 0.0008 | 0.0011 | 0.0003 | 0.0001 | 0.0016 | 0.0003 | 0.0079 | 0.0012 | 0.0002 | 0.0005 | 0.0044 | 0.0002 |
Peroxisomes | 0.1228 | 0.0874 | 0.0567 | 0.0429 | 0.0341 | 0.056 | 0.0679 | 0.0558 | 0.0563 | 0.0891 | 0.0074 | 0.0656 | 0.0318 | 0.053 | 0.027 | 0.0355 | 0.0351 | 0.0449 |
Punctate Nuclear | 0.0071 | 0.0011 | 0.001 | 0.0016 | 0.0027 | 0.0008 | 0.0026 | 0.0028 | 0.0014 | 0.0002 | 0.0028 | 0.0053 | 0.0044 | 0.0005 | 0.0003 | 0.0007 | 0.0071 | 0.0004 |
Vacuole | 0.0019 | 0.0015 | 0.0005 | 0.0014 | 0.0002 | 0.0004 | 0.0008 | 0.0008 | 0.0007 | 0.0009 | 0.001 | 0.0001 | 0.0013 | 0.0015 | 0.0005 | 0.0047 | 0.0031 | 0.0007 |
Vacuole Periphery | 0.0005 | 0.0004 | 0.0002 | 0.0011 | 0.0002 | 0.0002 | 0.0007 | 0.0002 | 0.0002 | 0.0006 | 0.0006 | 0.0001 | 0.0017 | 0.0003 | 0.0002 | 0.0011 | 0.0008 | 0.0007 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 10.199 | 10.8268 | 20.2963 | 17.9249 | 19.0087 | 20.3867 | 19.5893 | 18.0823 | 17.4567 | 20.3336 |
Translational Efficiency | 1.7678 | 1.6791 | 0.8934 | 1.1317 | 1.0416 | 0.9628 | 1.0148 | 1.2834 | 1.1378 | 0.9514 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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